Polymorphobacter fuscus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingosinicellaceae; Polymorphobacter

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3101 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7C9KKI3|A0A7C9KKI3_9SPHN TonB-dependent receptor OS=Polymorphobacter fuscus OX=1439888 GN=F3168_04425 PE=3 SV=1
MM1 pKa = 7.64ALFNFGGQTVDD12 pKa = 4.65SSDD15 pKa = 3.79ADD17 pKa = 3.68ALAQAAQDD25 pKa = 3.42AGATFGGSIVAGNTYY40 pKa = 9.85GVSGGVVTGTVHH52 pKa = 7.71GDD54 pKa = 3.57EE55 pKa = 4.22QHH57 pKa = 7.16DD58 pKa = 3.63AA59 pKa = 3.87

Molecular weight: 5.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7C9KWI1|A0A7C9KWI1_9SPHN Tryptophan-rich sensory protein OS=Polymorphobacter fuscus OX=1439888 GN=F3168_07005 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.43LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.38GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84NVIRR32 pKa = 11.84NRR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.26KK41 pKa = 10.65LSAA44 pKa = 3.91

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3101

0

3101

1023288

29

2830

330.0

35.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.897 ± 0.066

0.721 ± 0.012

6.021 ± 0.033

4.334 ± 0.04

3.496 ± 0.024

9.234 ± 0.062

1.896 ± 0.023

4.835 ± 0.025

2.514 ± 0.038

9.876 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.329 ± 0.023

2.456 ± 0.031

5.628 ± 0.039

2.842 ± 0.023

7.341 ± 0.043

4.816 ± 0.036

5.619 ± 0.036

7.652 ± 0.03

1.439 ± 0.019

2.054 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski