Newcastle disease virus (strain Chicken/United States/B1/48) (NDV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Paramyxoviridae; Avulavirinae; Orthoavulavirus; Avian orthoavulavirus 1

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q91UL0|HN_NDVB1 Hemagglutinin-neuraminidase OS=Newcastle disease virus (strain Chicken/United States/B1/48) OX=652953 GN=HN PE=1 SV=1
MM1 pKa = 7.45SSVFDD6 pKa = 4.24EE7 pKa = 4.58YY8 pKa = 11.32EE9 pKa = 3.81QLLAAQTRR17 pKa = 11.84PNGAHH22 pKa = 6.5GGGEE26 pKa = 3.98KK27 pKa = 10.82GSTLKK32 pKa = 10.74VDD34 pKa = 3.72VPVFTLNSDD43 pKa = 3.68DD44 pKa = 5.54PEE46 pKa = 4.53DD47 pKa = 3.22RR48 pKa = 11.84WSFVVFCLRR57 pKa = 11.84IAVSEE62 pKa = 4.28DD63 pKa = 3.01ANKK66 pKa = 10.05PLRR69 pKa = 11.84QGALISLLCSHH80 pKa = 6.17SQVMRR85 pKa = 11.84NHH87 pKa = 5.78VALAGKK93 pKa = 9.58QNEE96 pKa = 4.04ATLAVLEE103 pKa = 4.36IDD105 pKa = 4.13GFANGTPQFNNRR117 pKa = 11.84SGVSEE122 pKa = 3.88EE123 pKa = 3.83RR124 pKa = 11.84AQRR127 pKa = 11.84FAMIAGSLPRR137 pKa = 11.84ACSNGTPFVTAGAEE151 pKa = 3.83DD152 pKa = 4.74DD153 pKa = 4.08APEE156 pKa = 5.68DD157 pKa = 3.31ITDD160 pKa = 3.59TLEE163 pKa = 4.86RR164 pKa = 11.84ILSIQAQVWVTVAKK178 pKa = 10.9AMTAYY183 pKa = 7.35EE184 pKa = 4.12TADD187 pKa = 3.26EE188 pKa = 4.57SEE190 pKa = 3.98TRR192 pKa = 11.84RR193 pKa = 11.84INKK196 pKa = 9.01YY197 pKa = 6.52MQQGRR202 pKa = 11.84VQKK205 pKa = 10.55KK206 pKa = 7.89YY207 pKa = 10.65ILYY210 pKa = 7.91PVCRR214 pKa = 11.84STIQLTIRR222 pKa = 11.84QSLAVRR228 pKa = 11.84IFLVSEE234 pKa = 4.38LKK236 pKa = 10.46RR237 pKa = 11.84GRR239 pKa = 11.84NTAGGTSTYY248 pKa = 10.11YY249 pKa = 11.25NLVGDD254 pKa = 3.76VDD256 pKa = 4.37SYY258 pKa = 11.6IRR260 pKa = 11.84NTGLTAFFLTLKK272 pKa = 10.69YY273 pKa = 10.63GINTKK278 pKa = 9.69TSALALSSLSGDD290 pKa = 3.33IQKK293 pKa = 9.34MKK295 pKa = 10.85QLMRR299 pKa = 11.84LYY301 pKa = 10.82RR302 pKa = 11.84MKK304 pKa = 10.88GDD306 pKa = 3.4NAPYY310 pKa = 7.51MTLLGDD316 pKa = 3.71SDD318 pKa = 3.87QMSFAPAEE326 pKa = 4.05YY327 pKa = 9.98AQLYY331 pKa = 8.71SFAMGMASVLDD342 pKa = 4.0KK343 pKa = 11.15GTGKK347 pKa = 10.26YY348 pKa = 8.36QFAKK352 pKa = 10.8DD353 pKa = 3.85FMSTSFWRR361 pKa = 11.84LGVEE365 pKa = 4.05YY366 pKa = 10.28AQAQGSSINEE376 pKa = 3.69DD377 pKa = 2.85MAAEE381 pKa = 4.45LKK383 pKa = 8.96LTPAARR389 pKa = 11.84RR390 pKa = 11.84GLAAAAQRR398 pKa = 11.84VSEE401 pKa = 4.41VTSSIDD407 pKa = 3.23MPTQQVGVLTGLSEE421 pKa = 4.57GGSQALQGGSNRR433 pKa = 11.84SQGQPEE439 pKa = 4.24AGDD442 pKa = 4.17GEE444 pKa = 4.82TQFLDD449 pKa = 4.29LMRR452 pKa = 11.84AVANSMRR459 pKa = 11.84EE460 pKa = 4.25APNSAQGTPQSGPPPTPGPSQDD482 pKa = 4.47NDD484 pKa = 3.98TDD486 pKa = 2.94WGYY489 pKa = 11.97

Molecular weight: 53.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9DLD6|PHOSP_NDVB1 Phosphoprotein OS=Newcastle disease virus (strain Chicken/United States/B1/48) OX=652953 GN=P PE=3 SV=1
MM1 pKa = 7.99DD2 pKa = 4.33SSRR5 pKa = 11.84TIGLYY10 pKa = 10.09FDD12 pKa = 4.63SAHH15 pKa = 6.53SSSNLLAFPIVLQDD29 pKa = 3.17TGDD32 pKa = 3.69GKK34 pKa = 11.01KK35 pKa = 10.16QIAPQYY41 pKa = 9.71RR42 pKa = 11.84IQRR45 pKa = 11.84LDD47 pKa = 2.92LWTDD51 pKa = 3.2SKK53 pKa = 11.18EE54 pKa = 3.97DD55 pKa = 3.45SVFITTYY62 pKa = 10.97GFIFQVGNEE71 pKa = 3.98EE72 pKa = 4.1ATVGIIDD79 pKa = 4.73DD80 pKa = 3.78KK81 pKa = 11.11PKK83 pKa = 10.89RR84 pKa = 11.84EE85 pKa = 4.12LLSAAMLCLGSVPNTGDD102 pKa = 3.72LIEE105 pKa = 4.64LARR108 pKa = 11.84ACLTMMVTCKK118 pKa = 10.27KK119 pKa = 10.43SATNTEE125 pKa = 3.74RR126 pKa = 11.84MVFSVVQAPQVLQSCRR142 pKa = 11.84VVANKK147 pKa = 10.08YY148 pKa = 10.08SSVNAVKK155 pKa = 10.22HH156 pKa = 4.84VKK158 pKa = 10.26APEE161 pKa = 4.53KK162 pKa = 10.52IPGSGTLEE170 pKa = 3.93YY171 pKa = 10.56KK172 pKa = 10.96VNFVSLTVVPKK183 pKa = 10.37KK184 pKa = 10.45DD185 pKa = 3.43VYY187 pKa = 10.74KK188 pKa = 10.75IPAAVLKK195 pKa = 10.62ISGSSLYY202 pKa = 10.87NLALNVTINVEE213 pKa = 3.83VDD215 pKa = 3.04PRR217 pKa = 11.84SPLVKK222 pKa = 10.22SLSKK226 pKa = 10.64SDD228 pKa = 3.09SGYY231 pKa = 9.64YY232 pKa = 10.76ANLFLHH238 pKa = 6.98IGLMTTVDD246 pKa = 3.37RR247 pKa = 11.84KK248 pKa = 10.11GKK250 pKa = 10.33KK251 pKa = 7.01VTFDD255 pKa = 3.32KK256 pKa = 11.21LEE258 pKa = 4.05KK259 pKa = 10.22KK260 pKa = 9.79IRR262 pKa = 11.84SLDD265 pKa = 3.67LSVGLSDD272 pKa = 3.83VLGPSVLVKK281 pKa = 10.85ARR283 pKa = 11.84GARR286 pKa = 11.84TKK288 pKa = 10.73LLAPFFSSSGTACYY302 pKa = 9.57PIANASPQVAKK313 pKa = 10.63ILWSQTACLRR323 pKa = 11.84SVKK326 pKa = 10.15IIIQAGTQRR335 pKa = 11.84AVAVTADD342 pKa = 3.79HH343 pKa = 6.67EE344 pKa = 4.77VTSTKK349 pKa = 10.49LEE351 pKa = 4.33KK352 pKa = 10.74GHH354 pKa = 6.49TLAKK358 pKa = 10.37YY359 pKa = 10.7NPFKK363 pKa = 10.84KK364 pKa = 10.49

Molecular weight: 39.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

5000

179

2204

625.0

68.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.46 ± 0.67

1.68 ± 0.218

5.16 ± 0.276

4.82 ± 0.402

3.2 ± 0.483

6.04 ± 0.755

2.02 ± 0.488

6.0 ± 0.571

5.14 ± 0.489

10.04 ± 1.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.5 ± 0.215

4.64 ± 0.344

5.16 ± 0.663

4.34 ± 0.759

5.04 ± 0.466

9.2 ± 0.476

7.36 ± 0.687

6.28 ± 0.392

0.84 ± 0.133

3.08 ± 0.383
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski