Wickerhamomyces ciferrii (strain F-60-10 / ATCC 14091 / CBS 111 / JCM 3599 / NBRC 0793 / NRRL Y-1031) (Yeast) (Pichia ciferrii)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Phaffomycetaceae; Wickerhamomyces; Wickerhamomyces ciferrii

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6698 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K0KLB2|K0KLB2_WICCF Protein SEY1 OS=Wickerhamomyces ciferrii (strain F-60-10 / ATCC 14091 / CBS 111 / JCM 3599 / NBRC 0793 / NRRL Y-1031) OX=1206466 GN=SEY1 PE=3 SV=1
MM1 pKa = 7.71LPTLIALYY9 pKa = 10.38CSILTLPLVCGRR21 pKa = 11.84PPIKK25 pKa = 10.52LQTTTQEE32 pKa = 4.24PLTFTLNLTQFIHH45 pKa = 6.13CTNITRR51 pKa = 11.84DD52 pKa = 3.6QVSVWKK58 pKa = 10.45DD59 pKa = 2.98ADD61 pKa = 3.82EE62 pKa = 5.03SPLITPNNEE71 pKa = 3.35TSNYY75 pKa = 10.68DD76 pKa = 3.04EE77 pKa = 6.41DD78 pKa = 3.39IWLDD82 pKa = 3.73CVAEE86 pKa = 4.01QDD88 pKa = 3.48VGALVSVSDD97 pKa = 3.43GTYY100 pKa = 10.45FEE102 pKa = 6.24DD103 pKa = 4.25SDD105 pKa = 4.39TNVDD109 pKa = 3.86QEE111 pKa = 4.7SKK113 pKa = 11.06DD114 pKa = 3.2ILRR117 pKa = 11.84WKK119 pKa = 9.23GFRR122 pKa = 11.84YY123 pKa = 9.44FVANPDD129 pKa = 3.6EE130 pKa = 4.62LLLDD134 pKa = 3.88RR135 pKa = 11.84DD136 pKa = 4.05VPLVPGIFGNVFDD149 pKa = 4.6TACAVCCEE157 pKa = 4.48GEE159 pKa = 4.09QGQAA163 pKa = 3.24

Molecular weight: 18.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K0KMF4|K0KMF4_WICCF Uncharacterized protein OS=Wickerhamomyces ciferrii (strain F-60-10 / ATCC 14091 / CBS 111 / JCM 3599 / NBRC 0793 / NRRL Y-1031) OX=1206466 GN=BN7_2930 PE=4 SV=1
MM1 pKa = 7.55GNFRR5 pKa = 11.84FPVKK9 pKa = 10.61FKK11 pKa = 11.13LPSSYY16 pKa = 10.03INHH19 pKa = 6.82MILRR23 pKa = 11.84DD24 pKa = 3.64HH25 pKa = 7.7FKK27 pKa = 10.06RR28 pKa = 11.84LQFAEE33 pKa = 4.36HH34 pKa = 6.49EE35 pKa = 4.66VTSKK39 pKa = 10.26ALKK42 pKa = 9.96YY43 pKa = 9.89IARR46 pKa = 11.84NTQLPPRR53 pKa = 11.84ARR55 pKa = 11.84LEE57 pKa = 4.17AQLQLATMPNYY68 pKa = 9.37TRR70 pKa = 11.84YY71 pKa = 7.21TQVRR75 pKa = 11.84DD76 pKa = 3.17RR77 pKa = 11.84CVNSGTARR85 pKa = 11.84SVLKK89 pKa = 10.52KK90 pKa = 10.24FRR92 pKa = 11.84LNRR95 pKa = 11.84VSFDD99 pKa = 3.35PCC101 pKa = 4.89

Molecular weight: 11.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6698

0

6698

3252096

66

4944

485.5

54.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.915 ± 0.028

0.885 ± 0.011

6.135 ± 0.027

6.745 ± 0.036

4.69 ± 0.022

5.149 ± 0.027

2.025 ± 0.011

7.413 ± 0.032

7.645 ± 0.03

9.484 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.507 ± 0.011

6.567 ± 0.03

4.402 ± 0.024

4.42 ± 0.031

3.773 ± 0.019

9.042 ± 0.045

5.605 ± 0.021

5.162 ± 0.02

0.991 ± 0.01

3.445 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski