Pilibacter termitis
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2569 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1T4L156|A0A1T4L156_9ENTE Permease IIC component OS=Pilibacter termitis OX=263852 GN=SAMN02745116_00470 PE=4 SV=1
MM1 pKa = 7.16 TLAKK5 pKa = 9.51 IVYY8 pKa = 10.46 ASMTGNTEE16 pKa = 3.89 EE17 pKa = 4.35 IADD20 pKa = 3.64 IVAEE24 pKa = 4.06 AFEE27 pKa = 4.36 NLDD30 pKa = 3.7 LEE32 pKa = 4.55 VEE34 pKa = 3.89 IDD36 pKa = 3.33 EE37 pKa = 4.77 CSAVEE42 pKa = 4.92 ASDD45 pKa = 4.45 FEE47 pKa = 4.29 NADD50 pKa = 3.2 ICVVATYY57 pKa = 9.24 TYY59 pKa = 11.39 GDD61 pKa = 3.83 GEE63 pKa = 4.56 LPDD66 pKa = 4.96 EE67 pKa = 4.49 IVDD70 pKa = 4.52 FYY72 pKa = 11.78 EE73 pKa = 4.92 DD74 pKa = 3.85 LADD77 pKa = 3.66 VDD79 pKa = 4.64 LTGKK83 pKa = 10.21 IYY85 pKa = 10.03 GVCGSGDD92 pKa = 3.21 TFYY95 pKa = 11.45 DD96 pKa = 4.02 EE97 pKa = 4.62 FCKK100 pKa = 10.96 SVDD103 pKa = 3.98 DD104 pKa = 5.5 FDD106 pKa = 4.88 AQFAKK111 pKa = 10.16 TGATKK116 pKa = 9.1 GTEE119 pKa = 4.13 VVKK122 pKa = 10.96 VDD124 pKa = 5.19 LAAEE128 pKa = 4.53 DD129 pKa = 4.09 DD130 pKa = 5.01 DD131 pKa = 4.19 IVKK134 pKa = 10.77 LEE136 pKa = 4.03 EE137 pKa = 3.93 FAKK140 pKa = 10.89 NLAEE144 pKa = 4.23 KK145 pKa = 11.15 VNGG148 pKa = 3.72
Molecular weight: 16.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.791
IPC2_protein 3.592
IPC_protein 3.592
Toseland 3.376
ProMoST 3.745
Dawson 3.579
Bjellqvist 3.732
Wikipedia 3.503
Rodwell 3.414
Grimsley 3.287
Solomon 3.567
Lehninger 3.516
Nozaki 3.694
DTASelect 3.897
Thurlkill 3.427
EMBOSS 3.516
Sillero 3.706
Patrickios 1.799
IPC_peptide 3.554
IPC2_peptide 3.681
IPC2.peptide.svr19 3.691
Protein with the highest isoelectric point:
>tr|A0A1T4KHN4|A0A1T4KHN4_9ENTE Fe-S cluster assembly ATP-binding protein OS=Pilibacter termitis OX=263852 GN=SAMN02745116_00263 PE=3 SV=1
MM1 pKa = 7.92 PKK3 pKa = 10.14 QKK5 pKa = 7.59 THH7 pKa = 6.46 RR8 pKa = 11.84 ASAKK12 pKa = 9.04 RR13 pKa = 11.84 FKK15 pKa = 10.22 RR16 pKa = 11.84 TGKK19 pKa = 10.53 GGLKK23 pKa = 9.97 RR24 pKa = 11.84 FRR26 pKa = 11.84 AFTSHH31 pKa = 6.88 RR32 pKa = 11.84 FHH34 pKa = 7.22 GKK36 pKa = 5.85 TVKK39 pKa = 9.59 QRR41 pKa = 11.84 RR42 pKa = 11.84 QLRR45 pKa = 11.84 KK46 pKa = 9.6 ARR48 pKa = 11.84 MVHH51 pKa = 6.2 SGDD54 pKa = 3.53 FKK56 pKa = 11.05 RR57 pKa = 11.84 IRR59 pKa = 11.84 QMLSQMRR66 pKa = 3.7
Molecular weight: 7.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.461
IPC2_protein 11.008
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.232
Rodwell 12.515
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.237
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.078
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2569
0
2569
790524
39
3707
307.7
34.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.775 ± 0.051
0.635 ± 0.014
4.808 ± 0.038
8.278 ± 0.066
4.912 ± 0.045
6.408 ± 0.045
1.759 ± 0.023
7.331 ± 0.047
7.713 ± 0.049
9.753 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.615 ± 0.027
4.925 ± 0.05
3.115 ± 0.025
3.667 ± 0.029
3.914 ± 0.042
6.09 ± 0.042
5.838 ± 0.068
7.024 ± 0.038
0.898 ± 0.016
3.541 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here