Culex rhabdo-like virus
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Z2RTB8|A0A1Z2RTB8_9RHAB Glycoprotein OS=Culex rhabdo-like virus OX=2010277 PE=4 SV=1
MM1 pKa = 7.2 IHH3 pKa = 6.8 NSEE6 pKa = 4.25 LLANAFLMIALISWTTCEE24 pKa = 4.16 DD25 pKa = 3.01 QSHH28 pKa = 5.33 NMKK31 pKa = 10.21 ILFPTSSKK39 pKa = 10.69 IEE41 pKa = 3.84 WHH43 pKa = 6.77 DD44 pKa = 3.61 VSPSNLICSTARR56 pKa = 11.84 IHH58 pKa = 6.72 QNIAHH63 pKa = 6.64 GLSFVVNRR71 pKa = 11.84 PVIGKK76 pKa = 9.18 DD77 pKa = 3.16 PKK79 pKa = 10.65 ISGTLCVAIEE89 pKa = 4.03 LTTTCSKK96 pKa = 11.22 GFFGGVDD103 pKa = 3.6 VTLSTSPADD112 pKa = 3.93 LSEE115 pKa = 4.17 EE116 pKa = 4.04 NCRR119 pKa = 11.84 KK120 pKa = 10.12 EE121 pKa = 4.47 IASQNQGEE129 pKa = 4.54 FSSAEE134 pKa = 3.97 HH135 pKa = 6.02 PQPVCSWMKK144 pKa = 9.63 TSSTSRR150 pKa = 11.84 TVIHH154 pKa = 6.73 LSKK157 pKa = 10.1 MDD159 pKa = 3.47 AHH161 pKa = 6.94 YY162 pKa = 11.0 DD163 pKa = 3.73 PYY165 pKa = 11.47 SDD167 pKa = 4.16 TLFSSIFLSGKK178 pKa = 9.55 CRR180 pKa = 11.84 ASFCQTVFEE189 pKa = 4.14 NRR191 pKa = 11.84 LWIPQEE197 pKa = 4.11 SLTEE201 pKa = 4.12 YY202 pKa = 10.62 CSTEE206 pKa = 3.88 HH207 pKa = 6.71 LVEE210 pKa = 4.32 SSMMIYY216 pKa = 10.38 NSSSNGSSLWSPDD229 pKa = 2.86 FDD231 pKa = 3.36 ISEE234 pKa = 4.24 EE235 pKa = 4.01 EE236 pKa = 4.72 KK237 pKa = 9.93 PCIMNFCGQKK247 pKa = 10.27 GLRR250 pKa = 11.84 FASGEE255 pKa = 3.98 WVALSRR261 pKa = 11.84 QSIPKK266 pKa = 9.18 APWVGEE272 pKa = 4.09 HH273 pKa = 6.92 FYY275 pKa = 11.0 HH276 pKa = 7.51 LKK278 pKa = 10.87 DD279 pKa = 3.91 CDD281 pKa = 3.28 GHH283 pKa = 4.85 TTVNLVEE290 pKa = 3.91 AHH292 pKa = 5.8 QDD294 pKa = 3.23 LRR296 pKa = 11.84 HH297 pKa = 4.75 MTKK300 pKa = 10.01 VVLEE304 pKa = 4.11 EE305 pKa = 5.56 FIDD308 pKa = 4.17 EE309 pKa = 4.2 QCEE312 pKa = 4.01 MTVNKK317 pKa = 10.21 LKK319 pKa = 10.04 EE320 pKa = 3.98 GNMISRR326 pKa = 11.84 IEE328 pKa = 4.07 LQTLYY333 pKa = 10.36 PRR335 pKa = 11.84 TPGFHH340 pKa = 7.03 PIYY343 pKa = 10.02 RR344 pKa = 11.84 YY345 pKa = 7.48 TQGKK349 pKa = 8.81 FLMGMAHH356 pKa = 6.02 YY357 pKa = 9.48 SWVSIEE363 pKa = 3.96 SSEE366 pKa = 3.85 IFPYY370 pKa = 10.37 ISIRR374 pKa = 11.84 SSLNKK379 pKa = 10.21 SIEE382 pKa = 3.96 YY383 pKa = 10.46 SYY385 pKa = 8.35 WTMDD389 pKa = 2.86 NRR391 pKa = 11.84 TQIIDD396 pKa = 4.1 GPNGLYY402 pKa = 10.16 ILDD405 pKa = 3.83 HH406 pKa = 5.28 QLIYY410 pKa = 10.87 GLEE413 pKa = 4.03 EE414 pKa = 3.64 EE415 pKa = 4.46 QKK417 pKa = 10.43 FRR419 pKa = 11.84 RR420 pKa = 11.84 ILSKK424 pKa = 10.43 SSKK427 pKa = 10.12 HH428 pKa = 5.23 VFPILPEE435 pKa = 3.91 QKK437 pKa = 9.37 TNGQSFVRR445 pKa = 11.84 LLSHH449 pKa = 6.53 TSYY452 pKa = 11.2 SFSDD456 pKa = 3.8 DD457 pKa = 3.28 ASLVDD462 pKa = 4.92 VIWHH466 pKa = 6.32 PVLTCLIITAILLPSMIGIIWCTKK490 pKa = 8.19 NQVFIKK496 pKa = 9.94 KK497 pKa = 9.61 LKK499 pKa = 10.57 SFAQRR504 pKa = 11.84 DD505 pKa = 3.89 VEE507 pKa = 4.0 PSEE510 pKa = 5.42 DD511 pKa = 3.21 ISEE514 pKa = 4.54 AEE516 pKa = 3.88 GEE518 pKa = 4.47 YY519 pKa = 10.78 FNPP522 pKa = 4.24
Molecular weight: 59.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.404
IPC2_protein 5.41
IPC_protein 5.436
Toseland 5.766
ProMoST 5.741
Dawson 5.639
Bjellqvist 5.677
Wikipedia 5.614
Rodwell 5.614
Grimsley 5.881
Solomon 5.639
Lehninger 5.626
Nozaki 5.881
DTASelect 6.046
Thurlkill 6.02
EMBOSS 5.982
Sillero 5.97
Patrickios 3.668
IPC_peptide 5.652
IPC2_peptide 5.982
IPC2.peptide.svr19 5.92
Protein with the highest isoelectric point:
>tr|A0A1Z2RT82|A0A1Z2RT82_9RHAB Nucleocapsid protein OS=Culex rhabdo-like virus OX=2010277 PE=4 SV=1
MM1 pKa = 7.39 NNSTQIKK8 pKa = 9.75 KK9 pKa = 8.53 KK10 pKa = 8.03 TFNMCVSKK18 pKa = 11.04 VLLVNATIEE27 pKa = 3.98 IKK29 pKa = 10.6 YY30 pKa = 10.13 RR31 pKa = 11.84 NPPKK35 pKa = 9.49 TCKK38 pKa = 10.07 EE39 pKa = 4.32 LIHH42 pKa = 7.48 SLEE45 pKa = 4.52 LIKK48 pKa = 11.12 DD49 pKa = 3.53 GGNWYY54 pKa = 10.43 QSDD57 pKa = 3.68 QEE59 pKa = 4.69 FIFFFYY65 pKa = 10.42 ILSGYY70 pKa = 6.08 TCKK73 pKa = 10.21 HH74 pKa = 4.2 VHH76 pKa = 6.1 HH77 pKa = 7.16 LGGFHH82 pKa = 7.89 LYY84 pKa = 10.0 RR85 pKa = 11.84 GCISGAFEE93 pKa = 4.34 IKK95 pKa = 10.35 FKK97 pKa = 10.85 GISPRR102 pKa = 11.84 FPLTYY107 pKa = 9.44 EE108 pKa = 4.0 YY109 pKa = 10.49 QGEE112 pKa = 4.5 GLGMIPDD119 pKa = 4.08 TLISFSLVAEE129 pKa = 4.41 EE130 pKa = 4.83 SLVSPISLWGYY141 pKa = 9.04 IIKK144 pKa = 10.61 GSPSKK149 pKa = 10.5 YY150 pKa = 9.25 RR151 pKa = 11.84 KK152 pKa = 9.81 SNEE155 pKa = 3.93 IKK157 pKa = 10.05 WGIFDD162 pKa = 4.55 NFPLSVKK169 pKa = 10.41 GKK171 pKa = 10.35 DD172 pKa = 3.41 GIFTIRR178 pKa = 11.84 RR179 pKa = 11.84 KK180 pKa = 7.73 TT181 pKa = 3.26
Molecular weight: 20.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.786
IPC2_protein 8.741
IPC_protein 8.639
Toseland 9.311
ProMoST 9.121
Dawson 9.604
Bjellqvist 9.37
Wikipedia 9.765
Rodwell 9.882
Grimsley 9.663
Solomon 9.663
Lehninger 9.619
Nozaki 9.502
DTASelect 9.311
Thurlkill 9.458
EMBOSS 9.75
Sillero 9.575
Patrickios 4.673
IPC_peptide 9.648
IPC2_peptide 8.039
IPC2.peptide.svr19 7.802
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3665
181
2119
733.0
83.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.73 ± 0.798
1.583 ± 0.348
5.293 ± 0.533
6.276 ± 0.516
4.475 ± 0.41
5.812 ± 0.458
2.456 ± 0.26
6.603 ± 0.633
6.439 ± 0.749
9.168 ± 0.811
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.647 ± 0.221
4.529 ± 0.29
4.748 ± 0.465
3.356 ± 0.294
5.184 ± 0.609
8.759 ± 0.733
5.703 ± 0.274
5.921 ± 0.34
1.692 ± 0.201
3.629 ± 0.314
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here