Ocimum basilicum RNA virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Howeltoviricetes; Cryppavirales; Mitoviridae; Mitovirus; unclassified Mitovirus

Average proteome isoelectric point is 9.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A221C6B1|A0A221C6B1_9VIRU RNA-dependent RNA polymerase OS=Ocimum basilicum RNA virus 2 OX=2020287 PE=4 SV=1
MM1 pKa = 6.83TTLFTINRR9 pKa = 11.84ILRR12 pKa = 11.84DD13 pKa = 3.18RR14 pKa = 11.84SARR17 pKa = 11.84EE18 pKa = 3.46WRR20 pKa = 11.84RR21 pKa = 11.84LLEE24 pKa = 3.73QSRR27 pKa = 11.84RR28 pKa = 11.84LVGLFNKK35 pKa = 9.88AQLVVFGRR43 pKa = 11.84LAKK46 pKa = 9.52GHH48 pKa = 6.42CLGTLHH54 pKa = 6.77MSRR57 pKa = 11.84RR58 pKa = 11.84ILALYY63 pKa = 9.11RR64 pKa = 11.84KK65 pKa = 9.79NGALFTAKK73 pKa = 10.23YY74 pKa = 10.3LKK76 pKa = 9.88QCSHH80 pKa = 6.48AVLVYY85 pKa = 10.44VGSQGEE91 pKa = 4.57LLTNLSVPVSLTRR104 pKa = 11.84KK105 pKa = 8.33GLPRR109 pKa = 11.84IIPSMFRR116 pKa = 11.84KK117 pKa = 10.09RR118 pKa = 11.84IMQGDD123 pKa = 4.01RR124 pKa = 11.84EE125 pKa = 4.34VLRR128 pKa = 11.84LVLTVLSLYY137 pKa = 10.89RR138 pKa = 11.84LMKK141 pKa = 10.31VGPKK145 pKa = 7.79GWRR148 pKa = 11.84RR149 pKa = 11.84IAHH152 pKa = 5.31KK153 pKa = 9.12TIHH156 pKa = 6.14IPSYY160 pKa = 10.01RR161 pKa = 11.84PSEE164 pKa = 4.07EE165 pKa = 3.73TMEE168 pKa = 4.05WSKK171 pKa = 11.78KK172 pKa = 10.2LLTSGASMLAAYY184 pKa = 9.15VPKK187 pKa = 10.61YY188 pKa = 10.41RR189 pKa = 11.84EE190 pKa = 3.78LPINFGFSWYY200 pKa = 9.56PVFTSGPNTYY210 pKa = 10.03KK211 pKa = 10.82KK212 pKa = 9.97PDD214 pKa = 3.66EE215 pKa = 4.79ASLSARR221 pKa = 11.84ARR223 pKa = 11.84KK224 pKa = 9.23ALSAYY229 pKa = 9.61LAQRR233 pKa = 11.84RR234 pKa = 11.84RR235 pKa = 11.84KK236 pKa = 8.02GQKK239 pKa = 8.54ASKK242 pKa = 9.65YY243 pKa = 8.96SLTVFHH249 pKa = 6.82TLPVDD254 pKa = 3.68ATAAITLLKK263 pKa = 10.15PPQLSSLAAMFAHH276 pKa = 6.15SRR278 pKa = 11.84VFYY281 pKa = 10.45PLDD284 pKa = 4.22GYY286 pKa = 10.92DD287 pKa = 3.66RR288 pKa = 11.84PVEE291 pKa = 4.02KK292 pKa = 10.55SRR294 pKa = 11.84EE295 pKa = 4.18GVDD298 pKa = 3.59VIHH301 pKa = 6.77WFINDD306 pKa = 3.38LMVEE310 pKa = 4.31VAEE313 pKa = 4.34KK314 pKa = 10.44LWWPSMTTRR323 pKa = 11.84PEE325 pKa = 3.73SGRR328 pKa = 11.84FGLKK332 pKa = 10.42LEE334 pKa = 4.49GAGKK338 pKa = 10.26VRR340 pKa = 11.84VFAIPNPIFQRR351 pKa = 11.84FLKK354 pKa = 10.02PLHH357 pKa = 6.3DD358 pKa = 3.93WEE360 pKa = 4.43MSVLKK365 pKa = 10.65QLDD368 pKa = 3.38TDD370 pKa = 3.75GTYY373 pKa = 10.86DD374 pKa = 3.41QLRR377 pKa = 11.84PLHH380 pKa = 5.97RR381 pKa = 11.84LKK383 pKa = 10.83GKK385 pKa = 9.97RR386 pKa = 11.84VLYY389 pKa = 10.94SFDD392 pKa = 3.78LSAATDD398 pKa = 3.66MFPKK402 pKa = 10.4VLSASMLSGLFGDD415 pKa = 4.32EE416 pKa = 5.9FGAAWYY422 pKa = 10.65DD423 pKa = 3.59MMSNTAFRR431 pKa = 11.84SPEE434 pKa = 3.98RR435 pKa = 11.84LSSPLKK441 pKa = 8.98ARR443 pKa = 11.84VYY445 pKa = 10.37RR446 pKa = 11.84FTRR449 pKa = 11.84GQPLGFYY456 pKa = 10.41SSWPTFSLTHH466 pKa = 6.55HH467 pKa = 5.57MVVWLAAWRR476 pKa = 11.84VYY478 pKa = 9.93PGKK481 pKa = 10.61KK482 pKa = 9.28FWDD485 pKa = 3.81YY486 pKa = 12.0ALLGDD491 pKa = 5.51DD492 pKa = 4.89IVIADD497 pKa = 3.73EE498 pKa = 4.57AVALEE503 pKa = 4.36YY504 pKa = 10.54RR505 pKa = 11.84DD506 pKa = 4.32IMQQMGGVINMTKK519 pKa = 10.5SLISHH524 pKa = 6.82NGCCEE529 pKa = 3.52FAKK532 pKa = 10.32RR533 pKa = 11.84FMVNYY538 pKa = 9.54HH539 pKa = 6.37IGGGTDD545 pKa = 3.96CSPSSLPNILLAHH558 pKa = 6.67SSLAATTLKK567 pKa = 10.01TLGAEE572 pKa = 4.09YY573 pKa = 10.62SVTFRR578 pKa = 11.84LRR580 pKa = 11.84GAGYY584 pKa = 10.17RR585 pKa = 11.84VLSKK589 pKa = 9.9IDD591 pKa = 3.11RR592 pKa = 11.84GSPVRR597 pKa = 11.84TFQGLSRR604 pKa = 11.84RR605 pKa = 11.84WKK607 pKa = 9.79RR608 pKa = 11.84HH609 pKa = 3.53WLSLFSRR616 pKa = 11.84SGVQPLPLKK625 pKa = 10.41LWLVLPEE632 pKa = 4.58GGVLDD637 pKa = 4.8PYY639 pKa = 11.24LEE641 pKa = 4.61GVARR645 pKa = 11.84SYY647 pKa = 11.32LLEE650 pKa = 4.07RR651 pKa = 11.84AKK653 pKa = 10.81PKK655 pKa = 10.7DD656 pKa = 3.21IDD658 pKa = 3.73QQSVDD663 pKa = 3.53MVRR666 pKa = 11.84LFWEE670 pKa = 4.86GFEE673 pKa = 4.45DD674 pKa = 3.58TFEE677 pKa = 4.41RR678 pKa = 11.84LLITFVQQHH687 pKa = 6.08CAHH690 pKa = 5.8VAWWCTMVRR699 pKa = 11.84NFDD702 pKa = 3.91APLEE706 pKa = 4.33DD707 pKa = 5.59LLTIPQSAKK716 pKa = 10.54SLDD719 pKa = 3.54RR720 pKa = 11.84KK721 pKa = 9.19STEE724 pKa = 4.72EE725 pKa = 3.6IPEE728 pKa = 4.3TYY730 pKa = 10.57GPALRR735 pKa = 11.84LWDD738 pKa = 4.12VIRR741 pKa = 11.84ARR743 pKa = 11.84PKK745 pKa = 9.95PRR747 pKa = 11.84ALAARR752 pKa = 11.84DD753 pKa = 4.08FWVTWVGG760 pKa = 3.23

Molecular weight: 86.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A221C6B1|A0A221C6B1_9VIRU RNA-dependent RNA polymerase OS=Ocimum basilicum RNA virus 2 OX=2020287 PE=4 SV=1
MM1 pKa = 6.83TTLFTINRR9 pKa = 11.84ILRR12 pKa = 11.84DD13 pKa = 3.18RR14 pKa = 11.84SARR17 pKa = 11.84EE18 pKa = 3.46WRR20 pKa = 11.84RR21 pKa = 11.84LLEE24 pKa = 3.73QSRR27 pKa = 11.84RR28 pKa = 11.84LVGLFNKK35 pKa = 9.88AQLVVFGRR43 pKa = 11.84LAKK46 pKa = 9.52GHH48 pKa = 6.42CLGTLHH54 pKa = 6.77MSRR57 pKa = 11.84RR58 pKa = 11.84ILALYY63 pKa = 9.11RR64 pKa = 11.84KK65 pKa = 9.79NGALFTAKK73 pKa = 10.23YY74 pKa = 10.3LKK76 pKa = 9.88QCSHH80 pKa = 6.48AVLVYY85 pKa = 10.44VGSQGEE91 pKa = 4.57LLTNLSVPVSLTRR104 pKa = 11.84KK105 pKa = 8.33GLPRR109 pKa = 11.84IIPSMFRR116 pKa = 11.84KK117 pKa = 10.09RR118 pKa = 11.84IMQGDD123 pKa = 4.01RR124 pKa = 11.84EE125 pKa = 4.34VLRR128 pKa = 11.84LVLTVLSLYY137 pKa = 10.89RR138 pKa = 11.84LMKK141 pKa = 10.31VGPKK145 pKa = 7.79GWRR148 pKa = 11.84RR149 pKa = 11.84IAHH152 pKa = 5.31KK153 pKa = 9.12TIHH156 pKa = 6.14IPSYY160 pKa = 10.01RR161 pKa = 11.84PSEE164 pKa = 4.07EE165 pKa = 3.73TMEE168 pKa = 4.05WSKK171 pKa = 11.78KK172 pKa = 10.2LLTSGASMLAAYY184 pKa = 9.15VPKK187 pKa = 10.61YY188 pKa = 10.41RR189 pKa = 11.84EE190 pKa = 3.78LPINFGFSWYY200 pKa = 9.56PVFTSGPNTYY210 pKa = 10.03KK211 pKa = 10.82KK212 pKa = 9.97PDD214 pKa = 3.66EE215 pKa = 4.79ASLSARR221 pKa = 11.84ARR223 pKa = 11.84KK224 pKa = 9.23ALSAYY229 pKa = 9.61LAQRR233 pKa = 11.84RR234 pKa = 11.84RR235 pKa = 11.84KK236 pKa = 8.02GQKK239 pKa = 8.54ASKK242 pKa = 9.65YY243 pKa = 8.96SLTVFHH249 pKa = 6.82TLPVDD254 pKa = 3.68ATAAITLLKK263 pKa = 10.15PPQLSSLAAMFAHH276 pKa = 6.15SRR278 pKa = 11.84VFYY281 pKa = 10.45PLDD284 pKa = 4.22GYY286 pKa = 10.92DD287 pKa = 3.66RR288 pKa = 11.84PVEE291 pKa = 4.02KK292 pKa = 10.55SRR294 pKa = 11.84EE295 pKa = 4.18GVDD298 pKa = 3.59VIHH301 pKa = 6.77WFINDD306 pKa = 3.38LMVEE310 pKa = 4.31VAEE313 pKa = 4.34KK314 pKa = 10.44LWWPSMTTRR323 pKa = 11.84PEE325 pKa = 3.73SGRR328 pKa = 11.84FGLKK332 pKa = 10.42LEE334 pKa = 4.49GAGKK338 pKa = 10.26VRR340 pKa = 11.84VFAIPNPIFQRR351 pKa = 11.84FLKK354 pKa = 10.02PLHH357 pKa = 6.3DD358 pKa = 3.93WEE360 pKa = 4.43MSVLKK365 pKa = 10.65QLDD368 pKa = 3.38TDD370 pKa = 3.75GTYY373 pKa = 10.86DD374 pKa = 3.41QLRR377 pKa = 11.84PLHH380 pKa = 5.97RR381 pKa = 11.84LKK383 pKa = 10.83GKK385 pKa = 9.97RR386 pKa = 11.84VLYY389 pKa = 10.94SFDD392 pKa = 3.78LSAATDD398 pKa = 3.66MFPKK402 pKa = 10.4VLSASMLSGLFGDD415 pKa = 4.32EE416 pKa = 5.9FGAAWYY422 pKa = 10.65DD423 pKa = 3.59MMSNTAFRR431 pKa = 11.84SPEE434 pKa = 3.98RR435 pKa = 11.84LSSPLKK441 pKa = 8.98ARR443 pKa = 11.84VYY445 pKa = 10.37RR446 pKa = 11.84FTRR449 pKa = 11.84GQPLGFYY456 pKa = 10.41SSWPTFSLTHH466 pKa = 6.55HH467 pKa = 5.57MVVWLAAWRR476 pKa = 11.84VYY478 pKa = 9.93PGKK481 pKa = 10.61KK482 pKa = 9.28FWDD485 pKa = 3.81YY486 pKa = 12.0ALLGDD491 pKa = 5.51DD492 pKa = 4.89IVIADD497 pKa = 3.73EE498 pKa = 4.57AVALEE503 pKa = 4.36YY504 pKa = 10.54RR505 pKa = 11.84DD506 pKa = 4.32IMQQMGGVINMTKK519 pKa = 10.5SLISHH524 pKa = 6.82NGCCEE529 pKa = 3.52FAKK532 pKa = 10.32RR533 pKa = 11.84FMVNYY538 pKa = 9.54HH539 pKa = 6.37IGGGTDD545 pKa = 3.96CSPSSLPNILLAHH558 pKa = 6.67SSLAATTLKK567 pKa = 10.01TLGAEE572 pKa = 4.09YY573 pKa = 10.62SVTFRR578 pKa = 11.84LRR580 pKa = 11.84GAGYY584 pKa = 10.17RR585 pKa = 11.84VLSKK589 pKa = 9.9IDD591 pKa = 3.11RR592 pKa = 11.84GSPVRR597 pKa = 11.84TFQGLSRR604 pKa = 11.84RR605 pKa = 11.84WKK607 pKa = 9.79RR608 pKa = 11.84HH609 pKa = 3.53WLSLFSRR616 pKa = 11.84SGVQPLPLKK625 pKa = 10.41LWLVLPEE632 pKa = 4.58GGVLDD637 pKa = 4.8PYY639 pKa = 11.24LEE641 pKa = 4.61GVARR645 pKa = 11.84SYY647 pKa = 11.32LLEE650 pKa = 4.07RR651 pKa = 11.84AKK653 pKa = 10.81PKK655 pKa = 10.7DD656 pKa = 3.21IDD658 pKa = 3.73QQSVDD663 pKa = 3.53MVRR666 pKa = 11.84LFWEE670 pKa = 4.86GFEE673 pKa = 4.45DD674 pKa = 3.58TFEE677 pKa = 4.41RR678 pKa = 11.84LLITFVQQHH687 pKa = 6.08CAHH690 pKa = 5.8VAWWCTMVRR699 pKa = 11.84NFDD702 pKa = 3.91APLEE706 pKa = 4.33DD707 pKa = 5.59LLTIPQSAKK716 pKa = 10.54SLDD719 pKa = 3.54RR720 pKa = 11.84KK721 pKa = 9.19STEE724 pKa = 4.72EE725 pKa = 3.6IPEE728 pKa = 4.3TYY730 pKa = 10.57GPALRR735 pKa = 11.84LWDD738 pKa = 4.12VIRR741 pKa = 11.84ARR743 pKa = 11.84PKK745 pKa = 9.95PRR747 pKa = 11.84ALAARR752 pKa = 11.84DD753 pKa = 4.08FWVTWVGG760 pKa = 3.23

Molecular weight: 86.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

760

760

760

760.0

86.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.5 ± 0.0

0.921 ± 0.0

4.342 ± 0.0

4.211 ± 0.0

4.737 ± 0.0

6.316 ± 0.0

2.368 ± 0.0

3.684 ± 0.0

5.658 ± 0.0

12.5 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.895 ± 0.0

1.842 ± 0.0

5.526 ± 0.0

2.763 ± 0.0

8.684 ± 0.0

7.763 ± 0.0

5.263 ± 0.0

6.579 ± 0.0

2.895 ± 0.0

3.553 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski