Amino acid dipepetide frequency for Mycobacterium phage LastHope

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.801AlaAla: 20.801 ± 1.93
1.153AlaCys: 1.153 ± 0.266
9.221AlaAsp: 9.221 ± 0.79
8.802AlaGlu: 8.802 ± 0.909
2.986AlaPhe: 2.986 ± 0.657
8.959AlaGly: 8.959 ± 0.768
3.51AlaHis: 3.51 ± 0.473
5.501AlaIle: 5.501 ± 0.67
2.672AlaLys: 2.672 ± 0.389
10.426AlaLeu: 10.426 ± 0.796
3.301AlaMet: 3.301 ± 0.365
2.829AlaAsn: 2.829 ± 0.448
6.916AlaPro: 6.916 ± 0.666
4.768AlaGln: 4.768 ± 0.566
9.117AlaArg: 9.117 ± 0.72
5.03AlaSer: 5.03 ± 0.658
7.44AlaThr: 7.44 ± 0.807
10.741AlaVal: 10.741 ± 0.725
2.253AlaTrp: 2.253 ± 0.277
2.567AlaTyr: 2.567 ± 0.483
0.0AlaXaa: 0.0 ± 0.0
Cys
0.838CysAla: 0.838 ± 0.207
0.105CysCys: 0.105 ± 0.07
0.472CysAsp: 0.472 ± 0.255
0.891CysGlu: 0.891 ± 0.28
0.262CysPhe: 0.262 ± 0.118
1.834CysGly: 1.834 ± 0.427
0.21CysHis: 0.21 ± 0.108
0.419CysIle: 0.419 ± 0.157
0.262CysLys: 0.262 ± 0.117
0.629CysLeu: 0.629 ± 0.178
0.21CysMet: 0.21 ± 0.105
0.157CysAsn: 0.157 ± 0.089
0.838CysPro: 0.838 ± 0.266
0.262CysGln: 0.262 ± 0.129
1.153CysArg: 1.153 ± 0.295
0.734CysSer: 0.734 ± 0.222
0.681CysThr: 0.681 ± 0.194
0.576CysVal: 0.576 ± 0.227
0.576CysTrp: 0.576 ± 0.155
0.157CysTyr: 0.157 ± 0.092
0.0CysXaa: 0.0 ± 0.0
Asp
7.597AspAla: 7.597 ± 0.82
0.838AspCys: 0.838 ± 0.254
5.921AspAsp: 5.921 ± 0.724
5.501AspGlu: 5.501 ± 0.528
1.257AspPhe: 1.257 ± 0.21
7.23AspGly: 7.23 ± 0.61
1.048AspHis: 1.048 ± 0.272
1.572AspIle: 1.572 ± 0.33
2.515AspLys: 2.515 ± 0.408
6.235AspLeu: 6.235 ± 0.665
1.939AspMet: 1.939 ± 0.381
1.834AspAsn: 1.834 ± 0.29
4.401AspPro: 4.401 ± 0.449
1.991AspGln: 1.991 ± 0.272
4.715AspArg: 4.715 ± 0.524
2.043AspSer: 2.043 ± 0.306
2.882AspThr: 2.882 ± 0.397
3.772AspVal: 3.772 ± 0.386
1.048AspTrp: 1.048 ± 0.24
1.572AspTyr: 1.572 ± 0.307
0.0AspXaa: 0.0 ± 0.0
Glu
8.697GluAla: 8.697 ± 0.813
0.943GluCys: 0.943 ± 0.272
2.201GluAsp: 2.201 ± 0.405
0.995GluGlu: 0.995 ± 0.252
2.253GluPhe: 2.253 ± 0.332
3.93GluGly: 3.93 ± 0.454
1.677GluHis: 1.677 ± 0.284
2.463GluIle: 2.463 ± 0.341
1.362GluLys: 1.362 ± 0.319
6.497GluLeu: 6.497 ± 0.661
1.205GluMet: 1.205 ± 0.265
1.1GluAsn: 1.1 ± 0.241
2.882GluPro: 2.882 ± 0.49
2.777GluGln: 2.777 ± 0.353
5.187GluArg: 5.187 ± 0.613
2.253GluSer: 2.253 ± 0.367
3.039GluThr: 3.039 ± 0.32
5.187GluVal: 5.187 ± 0.713
0.891GluTrp: 0.891 ± 0.253
1.781GluTyr: 1.781 ± 0.304
0.0GluXaa: 0.0 ± 0.0
Phe
3.144PheAla: 3.144 ± 0.529
0.314PheCys: 0.314 ± 0.124
3.091PheAsp: 3.091 ± 0.401
1.729PheGlu: 1.729 ± 0.302
0.419PhePhe: 0.419 ± 0.165
2.463PheGly: 2.463 ± 0.324
0.262PheHis: 0.262 ± 0.153
1.048PheIle: 1.048 ± 0.225
1.048PheLys: 1.048 ± 0.214
1.781PheLeu: 1.781 ± 0.366
0.576PheMet: 0.576 ± 0.147
1.1PheAsn: 1.1 ± 0.264
1.048PhePro: 1.048 ± 0.208
0.629PheGln: 0.629 ± 0.209
1.729PheArg: 1.729 ± 0.327
0.995PheSer: 0.995 ± 0.317
1.519PheThr: 1.519 ± 0.3
1.939PheVal: 1.939 ± 0.329
0.419PheTrp: 0.419 ± 0.141
0.472PheTyr: 0.472 ± 0.212
0.0PheXaa: 0.0 ± 0.0
Gly
10.426GlyAla: 10.426 ± 0.87
1.205GlyCys: 1.205 ± 0.339
4.977GlyAsp: 4.977 ± 0.582
5.554GlyGlu: 5.554 ± 0.519
1.939GlyPhe: 1.939 ± 0.317
9.221GlyGly: 9.221 ± 1.222
1.781GlyHis: 1.781 ± 0.354
2.672GlyIle: 2.672 ± 0.477
3.877GlyLys: 3.877 ± 0.381
7.44GlyLeu: 7.44 ± 0.721
1.991GlyMet: 1.991 ± 0.339
3.248GlyAsn: 3.248 ± 0.442
3.668GlyPro: 3.668 ± 0.572
2.725GlyGln: 2.725 ± 0.646
5.606GlyArg: 5.606 ± 0.568
5.03GlySer: 5.03 ± 0.648
6.287GlyThr: 6.287 ± 0.607
7.702GlyVal: 7.702 ± 0.612
2.305GlyTrp: 2.305 ± 0.345
2.62GlyTyr: 2.62 ± 0.524
0.0GlyXaa: 0.0 ± 0.0
His
2.358HisAla: 2.358 ± 0.401
0.314HisCys: 0.314 ± 0.14
1.31HisAsp: 1.31 ± 0.34
1.415HisGlu: 1.415 ± 0.289
0.367HisPhe: 0.367 ± 0.157
2.672HisGly: 2.672 ± 0.427
0.419HisHis: 0.419 ± 0.149
0.786HisIle: 0.786 ± 0.182
0.367HisLys: 0.367 ± 0.125
2.201HisLeu: 2.201 ± 0.362
0.314HisMet: 0.314 ± 0.138
0.576HisAsn: 0.576 ± 0.187
1.31HisPro: 1.31 ± 0.233
0.314HisGln: 0.314 ± 0.175
1.572HisArg: 1.572 ± 0.293
0.995HisSer: 0.995 ± 0.213
1.939HisThr: 1.939 ± 0.355
2.253HisVal: 2.253 ± 0.352
0.419HisTrp: 0.419 ± 0.175
0.629HisTyr: 0.629 ± 0.169
0.0HisXaa: 0.0 ± 0.0
Ile
6.025IleAla: 6.025 ± 0.569
0.105IleCys: 0.105 ± 0.08
3.668IleAsp: 3.668 ± 0.478
2.986IleGlu: 2.986 ± 0.449
0.786IlePhe: 0.786 ± 0.219
4.296IleGly: 4.296 ± 0.815
0.472IleHis: 0.472 ± 0.161
0.891IleIle: 0.891 ± 0.23
1.467IleLys: 1.467 ± 0.278
2.829IleLeu: 2.829 ± 0.391
0.419IleMet: 0.419 ± 0.156
1.257IleAsn: 1.257 ± 0.248
1.624IlePro: 1.624 ± 0.355
0.734IleGln: 0.734 ± 0.243
2.358IleArg: 2.358 ± 0.327
1.572IleSer: 1.572 ± 0.243
2.41IleThr: 2.41 ± 0.286
3.72IleVal: 3.72 ± 0.513
0.734IleTrp: 0.734 ± 0.194
0.524IleTyr: 0.524 ± 0.174
0.0IleXaa: 0.0 ± 0.0
Lys
3.668LysAla: 3.668 ± 0.449
0.472LysCys: 0.472 ± 0.144
1.048LysAsp: 1.048 ± 0.24
0.524LysGlu: 0.524 ± 0.153
1.257LysPhe: 1.257 ± 0.27
2.934LysGly: 2.934 ± 0.464
0.681LysHis: 0.681 ± 0.166
1.991LysIle: 1.991 ± 0.42
0.681LysLys: 0.681 ± 0.226
3.144LysLeu: 3.144 ± 0.357
0.891LysMet: 0.891 ± 0.209
0.524LysAsn: 0.524 ± 0.169
2.41LysPro: 2.41 ± 0.4
0.629LysGln: 0.629 ± 0.146
2.829LysArg: 2.829 ± 0.376
1.1LysSer: 1.1 ± 0.228
1.519LysThr: 1.519 ± 0.263
2.725LysVal: 2.725 ± 0.406
0.472LysTrp: 0.472 ± 0.129
1.153LysTyr: 1.153 ± 0.291
0.0LysXaa: 0.0 ± 0.0
Leu
12.784LeuAla: 12.784 ± 0.809
0.943LeuCys: 0.943 ± 0.238
7.492LeuAsp: 7.492 ± 0.632
2.305LeuGlu: 2.305 ± 0.361
1.939LeuPhe: 1.939 ± 0.307
6.706LeuGly: 6.706 ± 0.632
2.096LeuHis: 2.096 ± 0.317
3.458LeuIle: 3.458 ± 0.395
2.515LeuLys: 2.515 ± 0.317
5.659LeuLeu: 5.659 ± 0.668
1.624LeuMet: 1.624 ± 0.244
1.677LeuAsn: 1.677 ± 0.262
4.139LeuPro: 4.139 ± 0.541
2.41LeuGln: 2.41 ± 0.377
6.864LeuArg: 6.864 ± 0.583
5.239LeuSer: 5.239 ± 0.389
4.82LeuThr: 4.82 ± 0.485
6.864LeuVal: 6.864 ± 0.614
1.415LeuTrp: 1.415 ± 0.257
1.1LeuTyr: 1.1 ± 0.301
0.0LeuXaa: 0.0 ± 0.0
Met
2.62MetAla: 2.62 ± 0.419
0.052MetCys: 0.052 ± 0.049
0.786MetAsp: 0.786 ± 0.177
0.734MetGlu: 0.734 ± 0.197
0.786MetPhe: 0.786 ± 0.228
1.624MetGly: 1.624 ± 0.276
0.734MetHis: 0.734 ± 0.209
1.257MetIle: 1.257 ± 0.275
0.524MetLys: 0.524 ± 0.174
1.572MetLeu: 1.572 ± 0.265
0.681MetMet: 0.681 ± 0.179
0.681MetAsn: 0.681 ± 0.198
1.257MetPro: 1.257 ± 0.259
0.734MetGln: 0.734 ± 0.195
1.467MetArg: 1.467 ± 0.261
2.829MetSer: 2.829 ± 0.362
1.939MetThr: 1.939 ± 0.382
1.467MetVal: 1.467 ± 0.295
0.419MetTrp: 0.419 ± 0.137
0.472MetTyr: 0.472 ± 0.167
0.0MetXaa: 0.0 ± 0.0
Asn
3.406AsnAla: 3.406 ± 0.479
0.314AsnCys: 0.314 ± 0.117
1.153AsnAsp: 1.153 ± 0.22
1.31AsnGlu: 1.31 ± 0.258
0.786AsnPhe: 0.786 ± 0.29
4.192AsnGly: 4.192 ± 0.557
0.524AsnHis: 0.524 ± 0.147
0.891AsnIle: 0.891 ± 0.244
0.576AsnLys: 0.576 ± 0.176
2.463AsnLeu: 2.463 ± 0.313
0.262AsnMet: 0.262 ± 0.097
0.943AsnAsn: 0.943 ± 0.282
2.253AsnPro: 2.253 ± 0.363
0.524AsnGln: 0.524 ± 0.147
1.572AsnArg: 1.572 ± 0.316
1.048AsnSer: 1.048 ± 0.237
1.677AsnThr: 1.677 ± 0.377
2.096AsnVal: 2.096 ± 0.361
0.419AsnTrp: 0.419 ± 0.15
0.472AsnTyr: 0.472 ± 0.154
0.0AsnXaa: 0.0 ± 0.0
Pro
7.23ProAla: 7.23 ± 0.506
0.524ProCys: 0.524 ± 0.184
3.248ProAsp: 3.248 ± 0.433
3.982ProGlu: 3.982 ± 0.454
1.624ProPhe: 1.624 ± 0.309
5.659ProGly: 5.659 ± 0.571
1.1ProHis: 1.1 ± 0.256
1.939ProIle: 1.939 ± 0.277
1.991ProLys: 1.991 ± 0.492
3.72ProLeu: 3.72 ± 0.494
1.257ProMet: 1.257 ± 0.278
1.415ProAsn: 1.415 ± 0.408
3.039ProPro: 3.039 ± 0.465
1.572ProGln: 1.572 ± 0.29
3.091ProArg: 3.091 ± 0.458
3.196ProSer: 3.196 ± 0.475
3.458ProThr: 3.458 ± 0.452
4.611ProVal: 4.611 ± 0.59
1.153ProTrp: 1.153 ± 0.242
1.519ProTyr: 1.519 ± 0.301
0.0ProXaa: 0.0 ± 0.0
Gln
4.087GlnAla: 4.087 ± 0.492
0.472GlnCys: 0.472 ± 0.15
1.205GlnAsp: 1.205 ± 0.273
0.891GlnGlu: 0.891 ± 0.233
1.153GlnPhe: 1.153 ± 0.246
2.358GlnGly: 2.358 ± 0.346
0.838GlnHis: 0.838 ± 0.206
1.624GlnIle: 1.624 ± 0.269
1.048GlnLys: 1.048 ± 0.218
2.148GlnLeu: 2.148 ± 0.367
1.467GlnMet: 1.467 ± 0.291
0.838GlnAsn: 0.838 ± 0.187
1.991GlnPro: 1.991 ± 0.323
1.362GlnGln: 1.362 ± 0.292
3.301GlnArg: 3.301 ± 0.43
1.31GlnSer: 1.31 ± 0.285
1.572GlnThr: 1.572 ± 0.278
3.353GlnVal: 3.353 ± 0.347
0.681GlnTrp: 0.681 ± 0.223
1.153GlnTyr: 1.153 ± 0.201
0.0GlnXaa: 0.0 ± 0.0
Arg
7.335ArgAla: 7.335 ± 0.676
0.891ArgCys: 0.891 ± 0.25
4.296ArgAsp: 4.296 ± 0.482
5.344ArgGlu: 5.344 ± 0.454
2.096ArgPhe: 2.096 ± 0.373
5.292ArgGly: 5.292 ± 0.588
1.939ArgHis: 1.939 ± 0.323
3.091ArgIle: 3.091 ± 0.369
3.091ArgLys: 3.091 ± 0.4
5.921ArgLeu: 5.921 ± 0.751
2.043ArgMet: 2.043 ± 0.305
2.358ArgAsn: 2.358 ± 0.445
3.668ArgPro: 3.668 ± 0.45
3.458ArgGln: 3.458 ± 0.44
6.287ArgArg: 6.287 ± 0.679
2.934ArgSer: 2.934 ± 0.358
3.825ArgThr: 3.825 ± 0.572
5.292ArgVal: 5.292 ± 0.521
1.939ArgTrp: 1.939 ± 0.303
2.096ArgTyr: 2.096 ± 0.399
0.0ArgXaa: 0.0 ± 0.0
Ser
6.549SerAla: 6.549 ± 0.752
0.262SerCys: 0.262 ± 0.105
2.62SerAsp: 2.62 ± 0.329
2.567SerGlu: 2.567 ± 0.352
1.205SerPhe: 1.205 ± 0.236
4.873SerGly: 4.873 ± 0.576
1.205SerHis: 1.205 ± 0.25
2.043SerIle: 2.043 ± 0.281
1.257SerLys: 1.257 ± 0.279
3.982SerLeu: 3.982 ± 0.468
1.31SerMet: 1.31 ± 0.25
1.153SerAsn: 1.153 ± 0.21
2.934SerPro: 2.934 ± 0.529
1.781SerGln: 1.781 ± 0.267
2.986SerArg: 2.986 ± 0.374
2.62SerSer: 2.62 ± 0.354
3.458SerThr: 3.458 ± 0.411
3.301SerVal: 3.301 ± 0.446
0.995SerTrp: 0.995 ± 0.244
1.257SerTyr: 1.257 ± 0.238
0.0SerXaa: 0.0 ± 0.0
Thr
7.021ThrAla: 7.021 ± 0.911
0.576ThrCys: 0.576 ± 0.199
3.825ThrAsp: 3.825 ± 0.511
3.877ThrGlu: 3.877 ± 0.54
1.939ThrPhe: 1.939 ± 0.267
5.187ThrGly: 5.187 ± 0.553
1.415ThrHis: 1.415 ± 0.299
2.672ThrIle: 2.672 ± 0.369
1.677ThrLys: 1.677 ± 0.259
4.401ThrLeu: 4.401 ± 0.435
0.734ThrMet: 0.734 ± 0.194
1.624ThrAsn: 1.624 ± 0.318
4.296ThrPro: 4.296 ± 0.473
1.677ThrGln: 1.677 ± 0.295
3.877ThrArg: 3.877 ± 0.519
3.039ThrSer: 3.039 ± 0.41
3.772ThrThr: 3.772 ± 0.502
5.711ThrVal: 5.711 ± 0.657
1.467ThrTrp: 1.467 ± 0.234
1.362ThrTyr: 1.362 ± 0.245
0.0ThrXaa: 0.0 ± 0.0
Val
10.112ValAla: 10.112 ± 0.947
1.257ValCys: 1.257 ± 0.262
6.654ValAsp: 6.654 ± 0.775
5.554ValGlu: 5.554 ± 0.706
1.624ValPhe: 1.624 ± 0.281
6.811ValGly: 6.811 ± 1.056
1.834ValHis: 1.834 ± 0.38
3.039ValIle: 3.039 ± 0.383
2.725ValLys: 2.725 ± 0.375
6.602ValLeu: 6.602 ± 0.571
1.519ValMet: 1.519 ± 0.244
1.939ValAsn: 1.939 ± 0.319
4.977ValPro: 4.977 ± 0.572
2.567ValGln: 2.567 ± 0.404
5.292ValArg: 5.292 ± 0.603
3.982ValSer: 3.982 ± 0.481
5.187ValThr: 5.187 ± 0.61
6.497ValVal: 6.497 ± 0.784
2.148ValTrp: 2.148 ± 0.327
1.834ValTyr: 1.834 ± 0.357
0.0ValXaa: 0.0 ± 0.0
Trp
1.991TrpAla: 1.991 ± 0.372
0.21TrpCys: 0.21 ± 0.102
0.786TrpAsp: 0.786 ± 0.235
0.786TrpGlu: 0.786 ± 0.177
0.367TrpPhe: 0.367 ± 0.127
1.467TrpGly: 1.467 ± 0.291
0.524TrpHis: 0.524 ± 0.176
0.995TrpIle: 0.995 ± 0.182
0.314TrpLys: 0.314 ± 0.131
2.829TrpLeu: 2.829 ± 0.318
0.21TrpMet: 0.21 ± 0.1
0.838TrpAsn: 0.838 ± 0.211
0.995TrpPro: 0.995 ± 0.229
0.995TrpGln: 0.995 ± 0.228
2.201TrpArg: 2.201 ± 0.381
1.048TrpSer: 1.048 ± 0.255
1.257TrpThr: 1.257 ± 0.258
1.939TrpVal: 1.939 ± 0.279
0.524TrpTrp: 0.524 ± 0.157
0.629TrpTyr: 0.629 ± 0.186
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.305TyrAla: 2.305 ± 0.252
0.314TyrCys: 0.314 ± 0.116
1.939TyrAsp: 1.939 ± 0.347
1.572TyrGlu: 1.572 ± 0.29
0.681TyrPhe: 0.681 ± 0.169
2.567TyrGly: 2.567 ± 0.415
0.262TyrHis: 0.262 ± 0.116
0.524TyrIle: 0.524 ± 0.166
0.681TyrLys: 0.681 ± 0.223
1.572TyrLeu: 1.572 ± 0.325
0.629TyrMet: 0.629 ± 0.159
0.786TyrAsn: 0.786 ± 0.172
0.681TyrPro: 0.681 ± 0.195
0.943TyrGln: 0.943 ± 0.256
1.991TyrArg: 1.991 ± 0.396
1.415TyrSer: 1.415 ± 0.293
1.467TyrThr: 1.467 ± 0.288
2.41TyrVal: 2.41 ± 0.419
0.629TyrTrp: 0.629 ± 0.224
0.576TyrTyr: 0.576 ± 0.132
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 102 proteins (19087 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski