Amino acid dipepetide frequency for Pusillimonas sp. JR1/69-2-13

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.66AlaAla: 13.66 ± 0.139	1.19AlaCys: 1.19 ± 0.031	5.909AlaAsp: 5.909 ± 0.07	5.428AlaGlu: 5.428 ± 0.07	3.774AlaPhe: 3.774 ± 0.063	9.551AlaGly: 9.551 ± 0.094	2.341AlaHis: 2.341 ± 0.041	6.271AlaIle: 6.271 ± 0.069	3.659AlaLys: 3.659 ± 0.073	12.809AlaLeu: 12.809 ± 0.131	3.345AlaMet: 3.345 ± 0.06	2.96AlaAsn: 2.96 ± 0.046	4.641AlaPro: 4.641 ± 0.078	4.959AlaGln: 4.959 ± 0.078	7.375AlaArg: 7.375 ± 0.079	6.726AlaSer: 6.726 ± 0.083	5.676AlaThr: 5.676 ± 0.068	8.052AlaVal: 8.052 ± 0.091	1.61AlaTrp: 1.61 ± 0.036	2.658AlaTyr: 2.658 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	1.077CysAla: 1.077 ± 0.031	0.138CysCys: 0.138 ± 0.011	0.505CysAsp: 0.505 ± 0.024	0.454CysGlu: 0.454 ± 0.018	0.311CysPhe: 0.311 ± 0.015	0.98CysGly: 0.98 ± 0.027	0.264CysHis: 0.264 ± 0.016	0.479CysIle: 0.479 ± 0.018	0.261CysLys: 0.261 ± 0.012	0.89CysLeu: 0.89 ± 0.027	0.248CysMet: 0.248 ± 0.013	0.276CysAsn: 0.276 ± 0.015	0.507CysPro: 0.507 ± 0.022	0.299CysGln: 0.299 ± 0.015	0.57CysArg: 0.57 ± 0.022	0.577CysSer: 0.577 ± 0.022	0.5CysThr: 0.5 ± 0.02	0.746CysVal: 0.746 ± 0.025	0.121CysTrp: 0.121 ± 0.01	0.222CysTyr: 0.222 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	6.001AspAla: 6.001 ± 0.069	0.489AspCys: 0.489 ± 0.021	2.827AspAsp: 2.827 ± 0.053	3.119AspGlu: 3.119 ± 0.052	2.094AspPhe: 2.094 ± 0.04	4.216AspGly: 4.216 ± 0.06	1.154AspHis: 1.154 ± 0.03	3.226AspIle: 3.226 ± 0.048	1.856AspLys: 1.856 ± 0.039	5.388AspLeu: 5.388 ± 0.069	1.389AspMet: 1.389 ± 0.033	1.357AspAsn: 1.357 ± 0.034	3.025AspPro: 3.025 ± 0.051	1.933AspGln: 1.933 ± 0.037	3.226AspArg: 3.226 ± 0.053	2.649AspSer: 2.649 ± 0.048	2.893AspThr: 2.893 ± 0.055	4.173AspVal: 4.173 ± 0.051	0.912AspTrp: 0.912 ± 0.028	1.592AspTyr: 1.592 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	5.539GluAla: 5.539 ± 0.073	0.44GluCys: 0.44 ± 0.02	2.237GluAsp: 2.237 ± 0.049	2.506GluGlu: 2.506 ± 0.052	1.897GluPhe: 1.897 ± 0.042	3.488GluGly: 3.488 ± 0.055	1.431GluHis: 1.431 ± 0.037	3.129GluIle: 3.129 ± 0.048	2.211GluLys: 2.211 ± 0.053	5.717GluLeu: 5.717 ± 0.073	1.348GluMet: 1.348 ± 0.037	1.752GluAsn: 1.752 ± 0.034	2.451GluPro: 2.451 ± 0.046	2.582GluGln: 2.582 ± 0.049	4.131GluArg: 4.131 ± 0.063	3.011GluSer: 3.011 ± 0.052	2.71GluThr: 2.71 ± 0.049	3.675GluVal: 3.675 ± 0.057	0.708GluTrp: 0.708 ± 0.024	1.225GluTyr: 1.225 ± 0.031	0.0GluXaa: 0.0 ± 0.0
Phe	3.74PheAla: 3.74 ± 0.062	0.411PheCys: 0.411 ± 0.018	2.403PheAsp: 2.403 ± 0.041	1.957PheGlu: 1.957 ± 0.038	1.412PhePhe: 1.412 ± 0.036	3.302PheGly: 3.302 ± 0.05	0.749PheHis: 0.749 ± 0.025	1.912PheIle: 1.912 ± 0.043	1.215PheLys: 1.215 ± 0.028	3.286PheLeu: 3.286 ± 0.057	0.936PheMet: 0.936 ± 0.026	1.258PheAsn: 1.258 ± 0.036	1.634PhePro: 1.634 ± 0.041	1.188PheGln: 1.188 ± 0.031	1.812PheArg: 1.812 ± 0.042	2.43PheSer: 2.43 ± 0.051	2.037PheThr: 2.037 ± 0.039	2.699PheVal: 2.699 ± 0.051	0.495PheTrp: 0.495 ± 0.025	0.943PheTyr: 0.943 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	7.922GlyAla: 7.922 ± 0.084	0.838GlyCys: 0.838 ± 0.028	3.716GlyAsp: 3.716 ± 0.062	3.982GlyGlu: 3.982 ± 0.054	3.123GlyPhe: 3.123 ± 0.052	6.302GlyGly: 6.302 ± 0.082	1.97GlyHis: 1.97 ± 0.041	4.831GlyIle: 4.831 ± 0.064	3.576GlyLys: 3.576 ± 0.054	8.781GlyLeu: 8.781 ± 0.085	2.578GlyMet: 2.578 ± 0.048	2.35GlyAsn: 2.35 ± 0.046	3.032GlyPro: 3.032 ± 0.048	3.157GlyGln: 3.157 ± 0.051	4.988GlyArg: 4.988 ± 0.069	4.82GlySer: 4.82 ± 0.066	4.499GlyThr: 4.499 ± 0.053	6.222GlyVal: 6.222 ± 0.073	1.261GlyTrp: 1.261 ± 0.036	2.516GlyTyr: 2.516 ± 0.049	0.0GlyXaa: 0.0 ± 0.0
His	2.684HisAla: 2.684 ± 0.051	0.263HisCys: 0.263 ± 0.014	1.249HisAsp: 1.249 ± 0.033	1.296HisGlu: 1.296 ± 0.033	0.837HisPhe: 0.837 ± 0.027	2.054HisGly: 2.054 ± 0.039	0.637HisHis: 0.637 ± 0.025	1.269HisIle: 1.269 ± 0.03	0.642HisLys: 0.642 ± 0.022	2.159HisLeu: 2.159 ± 0.04	0.563HisMet: 0.563 ± 0.021	0.639HisAsn: 0.639 ± 0.021	1.432HisPro: 1.432 ± 0.035	0.772HisGln: 0.772 ± 0.025	1.348HisArg: 1.348 ± 0.035	1.2HisSer: 1.2 ± 0.032	1.139HisThr: 1.139 ± 0.029	1.662HisVal: 1.662 ± 0.035	0.37HisTrp: 0.37 ± 0.017	0.671HisTyr: 0.671 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	6.667IleAla: 6.667 ± 0.074	0.555IleCys: 0.555 ± 0.018	3.492IleAsp: 3.492 ± 0.053	3.473IleGlu: 3.473 ± 0.058	1.76IlePhe: 1.76 ± 0.036	4.695IleGly: 4.695 ± 0.071	1.048IleHis: 1.048 ± 0.027	2.586IleIle: 2.586 ± 0.048	2.089IleLys: 2.089 ± 0.042	4.725IleLeu: 4.725 ± 0.077	1.242IleMet: 1.242 ± 0.035	1.904IleAsn: 1.904 ± 0.039	2.726IlePro: 2.726 ± 0.048	1.88IleGln: 1.88 ± 0.047	3.053IleArg: 3.053 ± 0.054	3.473IleSer: 3.473 ± 0.055	3.071IleThr: 3.071 ± 0.042	4.403IleVal: 4.403 ± 0.058	0.583IleTrp: 0.583 ± 0.019	1.139IleTyr: 1.139 ± 0.033	0.0IleXaa: 0.0 ± 0.0
Lys	4.023LysAla: 4.023 ± 0.062	0.18LysCys: 0.18 ± 0.013	1.821LysAsp: 1.821 ± 0.039	1.828LysGlu: 1.828 ± 0.041	1.012LysPhe: 1.012 ± 0.029	2.677LysGly: 2.677 ± 0.048	0.805LysHis: 0.805 ± 0.028	1.786LysIle: 1.786 ± 0.04	1.604LysLys: 1.604 ± 0.049	3.621LysLeu: 3.621 ± 0.065	0.923LysMet: 0.923 ± 0.025	1.185LysAsn: 1.185 ± 0.03	2.11LysPro: 2.11 ± 0.046	1.467LysGln: 1.467 ± 0.038	2.37LysArg: 2.37 ± 0.043	2.131LysSer: 2.131 ± 0.039	2.138LysThr: 2.138 ± 0.049	2.728LysVal: 2.728 ± 0.05	0.421LysTrp: 0.421 ± 0.02	0.779LysTyr: 0.779 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	13.288LeuAla: 13.288 ± 0.105	1.054LeuCys: 1.054 ± 0.029	5.919LeuAsp: 5.919 ± 0.069	5.307LeuGlu: 5.307 ± 0.063	3.664LeuPhe: 3.664 ± 0.068	8.421LeuGly: 8.421 ± 0.096	2.333LeuHis: 2.333 ± 0.05	5.445LeuIle: 5.445 ± 0.07	3.759LeuLys: 3.759 ± 0.063	10.789LeuLeu: 10.789 ± 0.143	2.566LeuMet: 2.566 ± 0.046	3.18LeuAsn: 3.18 ± 0.056	5.69LeuPro: 5.69 ± 0.072	3.827LeuGln: 3.827 ± 0.06	6.916LeuArg: 6.916 ± 0.083	6.773LeuSer: 6.773 ± 0.075	5.584LeuThr: 5.584 ± 0.062	7.586LeuVal: 7.586 ± 0.083	1.179LeuTrp: 1.179 ± 0.037	2.28LeuTyr: 2.28 ± 0.048	0.0LeuXaa: 0.0 ± 0.0
Met	3.1MetAla: 3.1 ± 0.052	0.188MetCys: 0.188 ± 0.011	1.226MetAsp: 1.226 ± 0.03	1.153MetGlu: 1.153 ± 0.028	0.815MetPhe: 0.815 ± 0.028	2.012MetGly: 2.012 ± 0.042	0.61MetHis: 0.61 ± 0.019	1.275MetIle: 1.275 ± 0.033	1.093MetLys: 1.093 ± 0.03	3.039MetLeu: 3.039 ± 0.053	0.685MetMet: 0.685 ± 0.025	0.992MetAsn: 0.992 ± 0.03	1.531MetPro: 1.531 ± 0.038	1.212MetGln: 1.212 ± 0.031	1.745MetArg: 1.745 ± 0.041	1.844MetSer: 1.844 ± 0.034	1.66MetThr: 1.66 ± 0.038	1.911MetVal: 1.911 ± 0.04	0.208MetTrp: 0.208 ± 0.015	0.454MetTyr: 0.454 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	3.364AsnAla: 3.364 ± 0.059	0.275AsnCys: 0.275 ± 0.015	1.593AsnAsp: 1.593 ± 0.041	1.543AsnGlu: 1.543 ± 0.033	1.098AsnPhe: 1.098 ± 0.026	2.436AsnGly: 2.436 ± 0.044	0.614AsnHis: 0.614 ± 0.02	1.72AsnIle: 1.72 ± 0.043	1.114AsnLys: 1.114 ± 0.03	2.919AsnLeu: 2.919 ± 0.05	0.773AsnMet: 0.773 ± 0.026	0.907AsnAsn: 0.907 ± 0.024	1.956AsnPro: 1.956 ± 0.041	1.086AsnGln: 1.086 ± 0.029	1.863AsnArg: 1.863 ± 0.038	1.536AsnSer: 1.536 ± 0.037	1.714AsnThr: 1.714 ± 0.038	2.195AsnVal: 2.195 ± 0.046	0.484AsnTrp: 0.484 ± 0.021	0.776AsnTyr: 0.776 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	5.667ProAla: 5.667 ± 0.074	0.388ProCys: 0.388 ± 0.018	3.432ProAsp: 3.432 ± 0.057	3.343ProGlu: 3.343 ± 0.052	1.821ProPhe: 1.821 ± 0.032	4.336ProGly: 4.336 ± 0.058	1.1ProHis: 1.1 ± 0.028	2.439ProIle: 2.439 ± 0.044	1.55ProLys: 1.55 ± 0.036	4.786ProLeu: 4.786 ± 0.07	1.288ProMet: 1.288 ± 0.033	1.397ProAsn: 1.397 ± 0.037	2.145ProPro: 2.145 ± 0.048	1.826ProGln: 1.826 ± 0.042	2.6ProArg: 2.6 ± 0.049	2.942ProSer: 2.942 ± 0.054	2.504ProThr: 2.504 ± 0.05	4.192ProVal: 4.192 ± 0.064	0.753ProTrp: 0.753 ± 0.027	1.405ProTyr: 1.405 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	5.196GlnAla: 5.196 ± 0.082	0.295GlnCys: 0.295 ± 0.016	1.783GlnAsp: 1.783 ± 0.042	2.023GlnGlu: 2.023 ± 0.045	1.256GlnPhe: 1.256 ± 0.029	2.999GlnGly: 2.999 ± 0.05	1.053GlnHis: 1.053 ± 0.027	2.116GlnIle: 2.116 ± 0.043	1.331GlnLys: 1.331 ± 0.033	4.086GlnLeu: 4.086 ± 0.062	0.985GlnMet: 0.985 ± 0.025	1.056GlnAsn: 1.056 ± 0.029	1.883GlnPro: 1.883 ± 0.036	1.953GlnGln: 1.953 ± 0.048	2.967GlnArg: 2.967 ± 0.057	2.152GlnSer: 2.152 ± 0.04	2.052GlnThr: 2.052 ± 0.038	2.879GlnVal: 2.879 ± 0.05	0.623GlnTrp: 0.623 ± 0.023	0.934GlnTyr: 0.934 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	6.173ArgAla: 6.173 ± 0.077	0.57ArgCys: 0.57 ± 0.022	3.401ArgAsp: 3.401 ± 0.052	3.552ArgGlu: 3.552 ± 0.058	2.423ArgPhe: 2.423 ± 0.045	4.11ArgGly: 4.11 ± 0.06	1.742ArgHis: 1.742 ± 0.038	3.846ArgIle: 3.846 ± 0.059	2.344ArgLys: 2.344 ± 0.044	7.155ArgLeu: 7.155 ± 0.081	1.841ArgMet: 1.841 ± 0.032	2.054ArgAsn: 2.054 ± 0.04	2.947ArgPro: 2.947 ± 0.053	2.934ArgGln: 2.934 ± 0.054	4.473ArgArg: 4.473 ± 0.068	3.742ArgSer: 3.742 ± 0.062	3.018ArgThr: 3.018 ± 0.049	4.562ArgVal: 4.562 ± 0.061	0.915ArgTrp: 0.915 ± 0.026	2.045ArgTyr: 2.045 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	6.301SerAla: 6.301 ± 0.071	0.535SerCys: 0.535 ± 0.021	3.057SerAsp: 3.057 ± 0.051	2.9SerGlu: 2.9 ± 0.051	2.353SerPhe: 2.353 ± 0.043	5.481SerGly: 5.481 ± 0.072	1.348SerHis: 1.348 ± 0.032	3.338SerIle: 3.338 ± 0.055	1.936SerLys: 1.936 ± 0.037	6.299SerLeu: 6.299 ± 0.075	1.753SerMet: 1.753 ± 0.038	1.797SerAsn: 1.797 ± 0.037	2.941SerPro: 2.941 ± 0.054	2.127SerGln: 2.127 ± 0.044	3.803SerArg: 3.803 ± 0.053	3.867SerSer: 3.867 ± 0.064	3.294SerThr: 3.294 ± 0.049	4.45SerVal: 4.45 ± 0.064	0.865SerTrp: 0.865 ± 0.028	1.486SerTyr: 1.486 ± 0.034	0.0SerXaa: 0.0 ± 0.0
Thr	5.522ThrAla: 5.522 ± 0.063	0.437ThrCys: 0.437 ± 0.021	2.791ThrAsp: 2.791 ± 0.046	2.605ThrGlu: 2.605 ± 0.037	1.737ThrPhe: 1.737 ± 0.036	4.796ThrGly: 4.796 ± 0.062	1.222ThrHis: 1.222 ± 0.032	2.926ThrIle: 2.926 ± 0.047	1.422ThrLys: 1.422 ± 0.039	6.298ThrLeu: 6.298 ± 0.08	1.4ThrMet: 1.4 ± 0.032	1.405ThrAsn: 1.405 ± 0.033	3.383ThrPro: 3.383 ± 0.059	2.016ThrGln: 2.016 ± 0.045	3.303ThrArg: 3.303 ± 0.05	3.07ThrSer: 3.07 ± 0.051	2.916ThrThr: 2.916 ± 0.047	4.251ThrVal: 4.251 ± 0.053	0.685ThrTrp: 0.685 ± 0.021	1.167ThrTyr: 1.167 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	8.76ValAla: 8.76 ± 0.082	0.736ValCys: 0.736 ± 0.026	4.108ValAsp: 4.108 ± 0.064	3.884ValGlu: 3.884 ± 0.06	2.886ValPhe: 2.886 ± 0.057	5.514ValGly: 5.514 ± 0.08	1.579ValHis: 1.579 ± 0.031	4.106ValIle: 4.106 ± 0.051	2.626ValLys: 2.626 ± 0.051	8.286ValLeu: 8.286 ± 0.099	1.937ValMet: 1.937 ± 0.038	2.391ValAsn: 2.391 ± 0.046	3.898ValPro: 3.898 ± 0.061	2.779ValGln: 2.779 ± 0.051	4.435ValArg: 4.435 ± 0.066	4.706ValSer: 4.706 ± 0.062	3.87ValThr: 3.87 ± 0.058	6.252ValVal: 6.252 ± 0.093	0.911ValTrp: 0.911 ± 0.028	1.672ValTyr: 1.672 ± 0.04	0.0ValXaa: 0.0 ± 0.0
Trp	1.212TrpAla: 1.212 ± 0.039	0.153TrpCys: 0.153 ± 0.011	0.598TrpAsp: 0.598 ± 0.022	0.546TrpGlu: 0.546 ± 0.022	0.562TrpPhe: 0.562 ± 0.024	0.878TrpGly: 0.878 ± 0.023	0.388TrpHis: 0.388 ± 0.018	0.752TrpIle: 0.752 ± 0.027	0.464TrpLys: 0.464 ± 0.022	1.948TrpLeu: 1.948 ± 0.041	0.392TrpMet: 0.392 ± 0.016	0.482TrpAsn: 0.482 ± 0.022	0.665TrpPro: 0.665 ± 0.027	0.671TrpGln: 0.671 ± 0.025	1.099TrpArg: 1.099 ± 0.033	0.826TrpSer: 0.826 ± 0.026	0.672TrpThr: 0.672 ± 0.026	0.892TrpVal: 0.892 ± 0.027	0.227TrpTrp: 0.227 ± 0.015	0.313TrpTyr: 0.313 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.697TyrAla: 2.697 ± 0.049	0.291TyrCys: 0.291 ± 0.016	1.452TyrAsp: 1.452 ± 0.037	1.288TyrGlu: 1.288 ± 0.033	0.986TyrPhe: 0.986 ± 0.026	2.216TyrGly: 2.216 ± 0.041	0.499TyrHis: 0.499 ± 0.018	1.112TyrIle: 1.112 ± 0.029	0.856TyrLys: 0.856 ± 0.029	2.578TyrLeu: 2.578 ± 0.052	0.56TyrMet: 0.56 ± 0.02	0.624TyrAsn: 0.624 ± 0.022	1.32TyrPro: 1.32 ± 0.037	0.954TyrGln: 0.954 ± 0.028	1.771TyrArg: 1.771 ± 0.039	1.458TyrSer: 1.458 ± 0.036	1.402TyrThr: 1.402 ± 0.036	1.807TyrVal: 1.807 ± 0.037	0.406TyrTrp: 0.406 ± 0.018	0.635TyrTyr: 0.635 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3981 proteins (1287452 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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