Amino acid dipepetide frequency for Rhodohalobacter sp. SW132

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.567AlaAla: 5.567 ± 0.091
0.511AlaCys: 0.511 ± 0.021
4.607AlaAsp: 4.607 ± 0.063
5.32AlaGlu: 5.32 ± 0.071
2.961AlaPhe: 2.961 ± 0.056
5.915AlaGly: 5.915 ± 0.08
1.237AlaHis: 1.237 ± 0.039
4.763AlaIle: 4.763 ± 0.073
2.768AlaLys: 2.768 ± 0.055
6.08AlaLeu: 6.08 ± 0.078
1.643AlaMet: 1.643 ± 0.039
2.658AlaAsn: 2.658 ± 0.05
2.123AlaPro: 2.123 ± 0.044
2.204AlaGln: 2.204 ± 0.042
3.142AlaArg: 3.142 ± 0.046
4.315AlaSer: 4.315 ± 0.062
3.389AlaThr: 3.389 ± 0.044
4.65AlaVal: 4.65 ± 0.062
0.807AlaTrp: 0.807 ± 0.026
2.236AlaTyr: 2.236 ± 0.039
0.0AlaXaa: 0.0 ± 0.0
Cys
0.379CysAla: 0.379 ± 0.018
0.077CysCys: 0.077 ± 0.008
0.383CysAsp: 0.383 ± 0.015
0.362CysGlu: 0.362 ± 0.018
0.275CysPhe: 0.275 ± 0.014
0.557CysGly: 0.557 ± 0.022
0.172CysHis: 0.172 ± 0.013
0.385CysIle: 0.385 ± 0.015
0.302CysLys: 0.302 ± 0.016
0.497CysLeu: 0.497 ± 0.023
0.136CysMet: 0.136 ± 0.009
0.254CysAsn: 0.254 ± 0.015
0.244CysPro: 0.244 ± 0.017
0.151CysGln: 0.151 ± 0.011
0.344CysArg: 0.344 ± 0.015
0.527CysSer: 0.527 ± 0.019
0.379CysThr: 0.379 ± 0.015
0.333CysVal: 0.333 ± 0.016
0.062CysTrp: 0.062 ± 0.006
0.206CysTyr: 0.206 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
3.95AspAla: 3.95 ± 0.051
0.298AspCys: 0.298 ± 0.014
3.993AspAsp: 3.993 ± 0.068
5.439AspGlu: 5.439 ± 0.066
3.117AspPhe: 3.117 ± 0.053
4.995AspGly: 4.995 ± 0.074
1.549AspHis: 1.549 ± 0.033
4.233AspIle: 4.233 ± 0.053
2.616AspLys: 2.616 ± 0.053
6.199AspLeu: 6.199 ± 0.069
1.359AspMet: 1.359 ± 0.031
2.408AspAsn: 2.408 ± 0.043
3.064AspPro: 3.064 ± 0.051
2.582AspGln: 2.582 ± 0.041
3.627AspArg: 3.627 ± 0.053
3.788AspSer: 3.788 ± 0.051
2.933AspThr: 2.933 ± 0.049
3.882AspVal: 3.882 ± 0.06
0.848AspTrp: 0.848 ± 0.025
2.243AspTyr: 2.243 ± 0.037
0.0AspXaa: 0.0 ± 0.0
Glu
4.887GluAla: 4.887 ± 0.067
0.327GluCys: 0.327 ± 0.019
4.121GluAsp: 4.121 ± 0.067
6.111GluGlu: 6.111 ± 0.091
3.293GluPhe: 3.293 ± 0.049
4.372GluGly: 4.372 ± 0.058
1.515GluHis: 1.515 ± 0.034
5.815GluIle: 5.815 ± 0.082
4.732GluLys: 4.732 ± 0.078
7.387GluLeu: 7.387 ± 0.072
2.041GluMet: 2.041 ± 0.044
4.411GluAsn: 4.411 ± 0.057
2.667GluPro: 2.667 ± 0.048
3.105GluGln: 3.105 ± 0.05
3.952GluArg: 3.952 ± 0.063
4.68GluSer: 4.68 ± 0.06
4.094GluThr: 4.094 ± 0.056
4.318GluVal: 4.318 ± 0.061
1.038GluTrp: 1.038 ± 0.03
2.589GluTyr: 2.589 ± 0.04
0.0GluXaa: 0.0 ± 0.0
Phe
2.99PheAla: 2.99 ± 0.053
0.345PheCys: 0.345 ± 0.016
3.391PheAsp: 3.391 ± 0.05
3.517PheGlu: 3.517 ± 0.052
2.281PhePhe: 2.281 ± 0.044
3.549PheGly: 3.549 ± 0.057
0.93PheHis: 0.93 ± 0.026
3.099PheIle: 3.099 ± 0.054
1.955PheLys: 1.955 ± 0.042
4.211PheLeu: 4.211 ± 0.064
1.108PheMet: 1.108 ± 0.03
2.434PheAsn: 2.434 ± 0.047
1.74PhePro: 1.74 ± 0.038
1.518PheGln: 1.518 ± 0.035
2.516PheArg: 2.516 ± 0.049
3.898PheSer: 3.898 ± 0.064
3.091PheThr: 3.091 ± 0.052
2.674PheVal: 2.674 ± 0.043
0.654PheTrp: 0.654 ± 0.025
1.69PheTyr: 1.69 ± 0.036
0.0PheXaa: 0.0 ± 0.0
Gly
4.554GlyAla: 4.554 ± 0.069
0.54GlyCys: 0.54 ± 0.021
4.517GlyAsp: 4.517 ± 0.071
5.228GlyGlu: 5.228 ± 0.067
3.742GlyPhe: 3.742 ± 0.062
5.324GlyGly: 5.324 ± 0.085
1.441GlyHis: 1.441 ± 0.038
5.399GlyIle: 5.399 ± 0.069
3.458GlyLys: 3.458 ± 0.059
6.338GlyLeu: 6.338 ± 0.082
1.99GlyMet: 1.99 ± 0.04
3.491GlyAsn: 3.491 ± 0.069
1.898GlyPro: 1.898 ± 0.037
2.231GlyGln: 2.231 ± 0.049
3.495GlyArg: 3.495 ± 0.05
5.132GlySer: 5.132 ± 0.076
4.362GlyThr: 4.362 ± 0.07
4.819GlyVal: 4.819 ± 0.062
1.16GlyTrp: 1.16 ± 0.034
2.822GlyTyr: 2.822 ± 0.053
0.0GlyXaa: 0.0 ± 0.0
His
1.332HisAla: 1.332 ± 0.037
0.167HisCys: 0.167 ± 0.012
1.106HisAsp: 1.106 ± 0.028
1.371HisGlu: 1.371 ± 0.031
1.169HisPhe: 1.169 ± 0.027
1.432HisGly: 1.432 ± 0.035
0.707HisHis: 0.707 ± 0.022
1.401HisIle: 1.401 ± 0.037
0.878HisLys: 0.878 ± 0.025
2.115HisLeu: 2.115 ± 0.043
0.408HisMet: 0.408 ± 0.017
0.945HisAsn: 0.945 ± 0.025
1.262HisPro: 1.262 ± 0.031
0.872HisGln: 0.872 ± 0.026
1.121HisArg: 1.121 ± 0.029
1.511HisSer: 1.511 ± 0.035
1.209HisThr: 1.209 ± 0.031
1.122HisVal: 1.122 ± 0.031
0.295HisTrp: 0.295 ± 0.015
0.91HisTyr: 0.91 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
4.992IleAla: 4.992 ± 0.074
0.538IleCys: 0.538 ± 0.022
4.46IleAsp: 4.46 ± 0.059
5.439IleGlu: 5.439 ± 0.076
3.079IlePhe: 3.079 ± 0.049
4.889IleGly: 4.889 ± 0.073
1.667IleHis: 1.667 ± 0.037
4.366IleIle: 4.366 ± 0.07
3.132IleLys: 3.132 ± 0.056
6.365IleLeu: 6.365 ± 0.078
1.27IleMet: 1.27 ± 0.031
3.289IleAsn: 3.289 ± 0.05
3.231IlePro: 3.231 ± 0.043
2.568IleGln: 2.568 ± 0.044
3.935IleArg: 3.935 ± 0.05
5.373IleSer: 5.373 ± 0.067
4.435IleThr: 4.435 ± 0.068
4.016IleVal: 4.016 ± 0.07
0.835IleTrp: 0.835 ± 0.029
2.411IleTyr: 2.411 ± 0.046
0.0IleXaa: 0.0 ± 0.0
Lys
3.13LysAla: 3.13 ± 0.068
0.206LysCys: 0.206 ± 0.012
2.476LysAsp: 2.476 ± 0.048
4.083LysGlu: 4.083 ± 0.078
1.686LysPhe: 1.686 ± 0.038
2.748LysGly: 2.748 ± 0.055
1.04LysHis: 1.04 ± 0.025
3.656LysIle: 3.656 ± 0.063
4.065LysLys: 4.065 ± 0.091
4.518LysLeu: 4.518 ± 0.072
1.428LysMet: 1.428 ± 0.032
2.833LysAsn: 2.833 ± 0.053
2.025LysPro: 2.025 ± 0.042
1.831LysGln: 1.831 ± 0.041
2.492LysArg: 2.492 ± 0.051
3.332LysSer: 3.332 ± 0.056
2.87LysThr: 2.87 ± 0.052
2.737LysVal: 2.737 ± 0.049
0.588LysTrp: 0.588 ± 0.022
1.538LysTyr: 1.538 ± 0.035
0.0LysXaa: 0.0 ± 0.0
Leu
6.073LeuAla: 6.073 ± 0.075
0.541LeuCys: 0.541 ± 0.021
5.586LeuAsp: 5.586 ± 0.068
6.402LeuGlu: 6.402 ± 0.064
4.942LeuPhe: 4.942 ± 0.071
5.682LeuGly: 5.682 ± 0.066
1.926LeuHis: 1.926 ± 0.033
6.669LeuIle: 6.669 ± 0.083
5.206LeuLys: 5.206 ± 0.075
8.693LeuLeu: 8.693 ± 0.104
2.271LeuMet: 2.271 ± 0.044
4.913LeuAsn: 4.913 ± 0.065
4.031LeuPro: 4.031 ± 0.056
3.448LeuGln: 3.448 ± 0.049
4.453LeuArg: 4.453 ± 0.058
6.807LeuSer: 6.807 ± 0.076
5.327LeuThr: 5.327 ± 0.071
5.133LeuVal: 5.133 ± 0.066
0.969LeuTrp: 0.969 ± 0.029
2.957LeuTyr: 2.957 ± 0.055
0.0LeuXaa: 0.0 ± 0.0
Met
1.812MetAla: 1.812 ± 0.035
0.113MetCys: 0.113 ± 0.008
1.515MetAsp: 1.515 ± 0.031
1.742MetGlu: 1.742 ± 0.034
0.891MetPhe: 0.891 ± 0.031
1.668MetGly: 1.668 ± 0.035
0.457MetHis: 0.457 ± 0.017
1.805MetIle: 1.805 ± 0.037
1.734MetLys: 1.734 ± 0.037
2.05MetLeu: 2.05 ± 0.04
0.76MetMet: 0.76 ± 0.027
1.387MetAsn: 1.387 ± 0.031
1.058MetPro: 1.058 ± 0.025
0.916MetGln: 0.916 ± 0.025
1.144MetArg: 1.144 ± 0.029
1.578MetSer: 1.578 ± 0.032
1.21MetThr: 1.21 ± 0.029
1.513MetVal: 1.513 ± 0.034
0.211MetTrp: 0.211 ± 0.013
0.639MetTyr: 0.639 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
2.911AsnAla: 2.911 ± 0.05
0.264AsnCys: 0.264 ± 0.014
2.542AsnAsp: 2.542 ± 0.048
3.319AsnGlu: 3.319 ± 0.053
2.072AsnPhe: 2.072 ± 0.04
3.73AsnGly: 3.73 ± 0.078
1.132AsnHis: 1.132 ± 0.028
3.323AsnIle: 3.323 ± 0.056
1.917AsnLys: 1.917 ± 0.042
4.609AsnLeu: 4.609 ± 0.06
1.091AsnMet: 1.091 ± 0.03
2.35AsnAsn: 2.35 ± 0.061
2.872AsnPro: 2.872 ± 0.049
2.026AsnGln: 2.026 ± 0.037
3.275AsnArg: 3.275 ± 0.056
3.29AsnSer: 3.29 ± 0.063
2.512AsnThr: 2.512 ± 0.048
2.879AsnVal: 2.879 ± 0.052
0.689AsnTrp: 0.689 ± 0.025
1.879AsnTyr: 1.879 ± 0.04
0.0AsnXaa: 0.0 ± 0.0
Pro
2.873ProAla: 2.873 ± 0.045
0.19ProCys: 0.19 ± 0.01
3.79ProAsp: 3.79 ± 0.052
4.011ProGlu: 4.011 ± 0.055
2.09ProPhe: 2.09 ± 0.038
3.454ProGly: 3.454 ± 0.053
0.802ProHis: 0.802 ± 0.03
2.421ProIle: 2.421 ± 0.043
1.591ProLys: 1.591 ± 0.038
3.305ProLeu: 3.305 ± 0.049
0.83ProMet: 0.83 ± 0.022
1.782ProAsn: 1.782 ± 0.039
1.326ProPro: 1.326 ± 0.032
1.208ProGln: 1.208 ± 0.031
1.526ProArg: 1.526 ± 0.034
2.451ProSer: 2.451 ± 0.038
1.758ProThr: 1.758 ± 0.033
3.319ProVal: 3.319 ± 0.049
0.482ProTrp: 0.482 ± 0.017
1.385ProTyr: 1.385 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
2.385GlnAla: 2.385 ± 0.044
0.158GlnCys: 0.158 ± 0.01
1.816GlnAsp: 1.816 ± 0.038
2.222GlnGlu: 2.222 ± 0.043
1.847GlnPhe: 1.847 ± 0.035
1.94GlnGly: 1.94 ± 0.04
0.742GlnHis: 0.742 ± 0.022
2.778GlnIle: 2.778 ± 0.044
2.106GlnLys: 2.106 ± 0.045
3.505GlnLeu: 3.505 ± 0.048
0.981GlnMet: 0.981 ± 0.031
2.167GlnAsn: 2.167 ± 0.043
1.52GlnPro: 1.52 ± 0.03
1.758GlnGln: 1.758 ± 0.045
1.949GlnArg: 1.949 ± 0.045
2.456GlnSer: 2.456 ± 0.048
2.2GlnThr: 2.2 ± 0.043
2.109GlnVal: 2.109 ± 0.035
0.475GlnTrp: 0.475 ± 0.019
1.174GlnTyr: 1.174 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
3.144ArgAla: 3.144 ± 0.046
0.26ArgCys: 0.26 ± 0.014
3.181ArgAsp: 3.181 ± 0.053
4.008ArgGlu: 4.008 ± 0.053
2.809ArgPhe: 2.809 ± 0.048
3.103ArgGly: 3.103 ± 0.045
1.005ArgHis: 1.005 ± 0.028
3.78ArgIle: 3.78 ± 0.057
2.73ArgLys: 2.73 ± 0.052
4.7ArgLeu: 4.7 ± 0.066
1.428ArgMet: 1.428 ± 0.033
2.62ArgAsn: 2.62 ± 0.043
1.805ArgPro: 1.805 ± 0.039
1.888ArgGln: 1.888 ± 0.036
2.451ArgArg: 2.451 ± 0.048
3.44ArgSer: 3.44 ± 0.06
2.718ArgThr: 2.718 ± 0.045
3.342ArgVal: 3.342 ± 0.055
0.772ArgTrp: 0.772 ± 0.028
2.202ArgTyr: 2.202 ± 0.046
0.0ArgXaa: 0.0 ± 0.0
Ser
4.773SerAla: 4.773 ± 0.065
0.457SerCys: 0.457 ± 0.021
4.745SerAsp: 4.745 ± 0.062
5.154SerGlu: 5.154 ± 0.071
3.396SerPhe: 3.396 ± 0.058
6.131SerGly: 6.131 ± 0.086
1.33SerHis: 1.33 ± 0.031
4.724SerIle: 4.724 ± 0.064
2.919SerLys: 2.919 ± 0.051
6.044SerLeu: 6.044 ± 0.069
1.55SerMet: 1.55 ± 0.035
3.056SerAsn: 3.056 ± 0.054
2.735SerPro: 2.735 ± 0.041
2.11SerGln: 2.11 ± 0.043
3.349SerArg: 3.349 ± 0.053
5.002SerSer: 5.002 ± 0.081
3.435SerThr: 3.435 ± 0.055
4.825SerVal: 4.825 ± 0.066
0.893SerTrp: 0.893 ± 0.029
2.407SerTyr: 2.407 ± 0.041
0.0SerXaa: 0.0 ± 0.0
Thr
3.956ThrAla: 3.956 ± 0.07
0.275ThrCys: 0.275 ± 0.015
3.783ThrAsp: 3.783 ± 0.049
3.923ThrGlu: 3.923 ± 0.05
2.771ThrPhe: 2.771 ± 0.05
5.123ThrGly: 5.123 ± 0.065
1.135ThrHis: 1.135 ± 0.03
4.006ThrIle: 4.006 ± 0.056
2.05ThrLys: 2.05 ± 0.044
5.244ThrLeu: 5.244 ± 0.062
1.1ThrMet: 1.1 ± 0.028
2.284ThrAsn: 2.284 ± 0.047
2.515ThrPro: 2.515 ± 0.05
1.686ThrGln: 1.686 ± 0.039
2.517ThrArg: 2.517 ± 0.044
3.434ThrSer: 3.434 ± 0.045
3.013ThrThr: 3.013 ± 0.053
3.992ThrVal: 3.992 ± 0.064
0.653ThrTrp: 0.653 ± 0.023
1.906ThrTyr: 1.906 ± 0.039
0.0ThrXaa: 0.0 ± 0.0
Val
4.29ValAla: 4.29 ± 0.059
0.442ValCys: 0.442 ± 0.018
4.043ValAsp: 4.043 ± 0.058
4.618ValGlu: 4.618 ± 0.069
2.781ValPhe: 2.781 ± 0.044
3.893ValGly: 3.893 ± 0.058
1.363ValHis: 1.363 ± 0.036
4.553ValIle: 4.553 ± 0.065
2.957ValLys: 2.957 ± 0.055
5.61ValLeu: 5.61 ± 0.07
1.641ValMet: 1.641 ± 0.036
3.009ValAsn: 3.009 ± 0.048
2.507ValPro: 2.507 ± 0.043
2.27ValGln: 2.27 ± 0.041
3.175ValArg: 3.175 ± 0.052
4.534ValSer: 4.534 ± 0.064
3.73ValThr: 3.73 ± 0.063
4.175ValVal: 4.175 ± 0.061
0.722ValTrp: 0.722 ± 0.023
2.11ValTyr: 2.11 ± 0.043
0.0ValXaa: 0.0 ± 0.0
Trp
0.774TrpAla: 0.774 ± 0.028
0.08TrpCys: 0.08 ± 0.008
0.825TrpAsp: 0.825 ± 0.028
0.839TrpGlu: 0.839 ± 0.031
0.641TrpPhe: 0.641 ± 0.023
0.899TrpGly: 0.899 ± 0.025
0.309TrpHis: 0.309 ± 0.015
0.982TrpIle: 0.982 ± 0.033
0.635TrpLys: 0.635 ± 0.021
1.232TrpLeu: 1.232 ± 0.036
0.429TrpMet: 0.429 ± 0.016
0.699TrpAsn: 0.699 ± 0.027
0.435TrpPro: 0.435 ± 0.018
0.533TrpGln: 0.533 ± 0.021
0.618TrpArg: 0.618 ± 0.02
0.852TrpSer: 0.852 ± 0.031
0.652TrpThr: 0.652 ± 0.021
0.759TrpVal: 0.759 ± 0.025
0.228TrpTrp: 0.228 ± 0.014
0.46TrpTyr: 0.46 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.124TyrAla: 2.124 ± 0.04
0.232TyrCys: 0.232 ± 0.013
2.247TyrAsp: 2.247 ± 0.044
2.349TyrGlu: 2.349 ± 0.045
1.746TyrPhe: 1.746 ± 0.04
2.537TyrGly: 2.537 ± 0.048
0.873TyrHis: 0.873 ± 0.026
2.133TyrIle: 2.133 ± 0.039
1.535TyrLys: 1.535 ± 0.041
3.327TyrLeu: 3.327 ± 0.052
0.737TyrMet: 0.737 ± 0.022
1.695TyrAsn: 1.695 ± 0.036
1.537TyrPro: 1.537 ± 0.039
1.385TyrGln: 1.385 ± 0.031
2.304TyrArg: 2.304 ± 0.043
2.636TyrSer: 2.636 ± 0.047
2.047TyrThr: 2.047 ± 0.038
1.824TyrVal: 1.824 ± 0.04
0.499TyrTrp: 0.499 ± 0.019
1.405TyrTyr: 1.405 ± 0.039
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4092 proteins (1445183 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski