Amino acid dipepetide frequency for Prevotella sp. AM34-19LB

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.844AlaAla: 5.844 ± 0.115	0.98AlaCys: 0.98 ± 0.036	4.378AlaAsp: 4.378 ± 0.079	4.872AlaGlu: 4.872 ± 0.092	3.153AlaPhe: 3.153 ± 0.068	4.783AlaGly: 4.783 ± 0.092	1.376AlaHis: 1.376 ± 0.036	4.902AlaIle: 4.902 ± 0.09	5.261AlaLys: 5.261 ± 0.082	6.507AlaLeu: 6.507 ± 0.098	2.303AlaMet: 2.303 ± 0.055	3.418AlaAsn: 3.418 ± 0.071	2.189AlaPro: 2.189 ± 0.056	3.062AlaGln: 3.062 ± 0.068	2.977AlaArg: 2.977 ± 0.063	4.435AlaSer: 4.435 ± 0.074	3.827AlaThr: 3.827 ± 0.077	4.39AlaVal: 4.39 ± 0.084	0.887AlaTrp: 0.887 ± 0.033	2.908AlaTyr: 2.908 ± 0.062	0.0AlaXaa: 0.0 ± 0.0
Cys	0.864CysAla: 0.864 ± 0.029	0.218CysCys: 0.218 ± 0.018	0.781CysAsp: 0.781 ± 0.03	0.725CysGlu: 0.725 ± 0.03	0.655CysPhe: 0.655 ± 0.029	1.084CysGly: 1.084 ± 0.04	0.295CysHis: 0.295 ± 0.02	0.942CysIle: 0.942 ± 0.036	0.876CysLys: 0.876 ± 0.032	1.141CysLeu: 1.141 ± 0.04	0.373CysMet: 0.373 ± 0.021	0.643CysAsn: 0.643 ± 0.026	0.502CysPro: 0.502 ± 0.026	0.435CysGln: 0.435 ± 0.025	0.589CysArg: 0.589 ± 0.033	0.8CysSer: 0.8 ± 0.031	0.643CysThr: 0.643 ± 0.031	0.797CysVal: 0.797 ± 0.034	0.183CysTrp: 0.183 ± 0.016	0.609CysTyr: 0.609 ± 0.026	0.0CysXaa: 0.0 ± 0.0
Asp	4.396AspAla: 4.396 ± 0.07	0.729AspCys: 0.729 ± 0.03	3.249AspAsp: 3.249 ± 0.07	4.423AspGlu: 4.423 ± 0.087	3.169AspPhe: 3.169 ± 0.066	4.185AspGly: 4.185 ± 0.091	1.101AspHis: 1.101 ± 0.037	4.173AspIle: 4.173 ± 0.076	4.392AspLys: 4.392 ± 0.069	4.577AspLeu: 4.577 ± 0.075	1.701AspMet: 1.701 ± 0.054	2.854AspAsn: 2.854 ± 0.07	1.773AspPro: 1.773 ± 0.045	1.535AspGln: 1.535 ± 0.039	2.408AspArg: 2.408 ± 0.054	3.226AspSer: 3.226 ± 0.072	2.786AspThr: 2.786 ± 0.073	3.772AspVal: 3.772 ± 0.076	0.9AspTrp: 0.9 ± 0.032	3.008AspTyr: 3.008 ± 0.053	0.0AspXaa: 0.0 ± 0.0
Glu	4.798GluAla: 4.798 ± 0.086	0.702GluCys: 0.702 ± 0.03	3.702GluAsp: 3.702 ± 0.077	5.208GluGlu: 5.208 ± 0.098	2.499GluPhe: 2.499 ± 0.055	4.177GluGly: 4.177 ± 0.067	1.423GluHis: 1.423 ± 0.045	4.31GluIle: 4.31 ± 0.076	5.552GluLys: 5.552 ± 0.105	5.641GluLeu: 5.641 ± 0.099	2.254GluMet: 2.254 ± 0.053	3.62GluAsn: 3.62 ± 0.071	1.714GluPro: 1.714 ± 0.041	2.771GluGln: 2.771 ± 0.07	3.044GluArg: 3.044 ± 0.073	3.036GluSer: 3.036 ± 0.063	3.09GluThr: 3.09 ± 0.067	4.125GluVal: 4.125 ± 0.072	0.827GluTrp: 0.827 ± 0.031	2.766GluTyr: 2.766 ± 0.051	0.0GluXaa: 0.0 ± 0.0
Phe	3.2PheAla: 3.2 ± 0.064	0.796PheCys: 0.796 ± 0.031	2.985PheAsp: 2.985 ± 0.061	2.543PheGlu: 2.543 ± 0.049	2.075PhePhe: 2.075 ± 0.062	3.228PheGly: 3.228 ± 0.071	0.889PheHis: 0.889 ± 0.034	2.792PheIle: 2.792 ± 0.066	2.62PheLys: 2.62 ± 0.057	3.834PheLeu: 3.834 ± 0.079	1.255PheMet: 1.255 ± 0.044	2.186PheAsn: 2.186 ± 0.059	1.529PhePro: 1.529 ± 0.045	1.291PheGln: 1.291 ± 0.04	1.956PheArg: 1.956 ± 0.048	3.181PheSer: 3.181 ± 0.071	2.679PheThr: 2.679 ± 0.055	2.751PheVal: 2.751 ± 0.058	0.538PheTrp: 0.538 ± 0.029	2.044PheTyr: 2.044 ± 0.056	0.0PheXaa: 0.0 ± 0.0
Gly	4.361GlyAla: 4.361 ± 0.09	1.042GlyCys: 1.042 ± 0.039	3.634GlyAsp: 3.634 ± 0.064	4.144GlyGlu: 4.144 ± 0.068	3.161GlyPhe: 3.161 ± 0.062	4.644GlyGly: 4.644 ± 0.106	1.243GlyHis: 1.243 ± 0.035	5.148GlyIle: 5.148 ± 0.085	6.091GlyLys: 6.091 ± 0.083	5.497GlyLeu: 5.497 ± 0.093	2.258GlyMet: 2.258 ± 0.059	3.5GlyAsn: 3.5 ± 0.085	1.21GlyPro: 1.21 ± 0.044	1.976GlyGln: 1.976 ± 0.048	2.772GlyArg: 2.772 ± 0.069	3.853GlySer: 3.853 ± 0.076	3.738GlyThr: 3.738 ± 0.081	4.616GlyVal: 4.616 ± 0.08	1.083GlyTrp: 1.083 ± 0.04	3.267GlyTyr: 3.267 ± 0.073	0.0GlyXaa: 0.0 ± 0.0
His	1.257HisAla: 1.257 ± 0.039	0.353HisCys: 0.353 ± 0.02	1.155HisAsp: 1.155 ± 0.038	1.119HisGlu: 1.119 ± 0.035	1.103HisPhe: 1.103 ± 0.032	1.346HisGly: 1.346 ± 0.045	0.638HisHis: 0.638 ± 0.03	1.613HisIle: 1.613 ± 0.045	1.148HisLys: 1.148 ± 0.035	2.002HisLeu: 2.002 ± 0.056	0.382HisMet: 0.382 ± 0.021	1.046HisAsn: 1.046 ± 0.035	1.058HisPro: 1.058 ± 0.038	0.873HisGln: 0.873 ± 0.031	1.025HisArg: 1.025 ± 0.036	1.192HisSer: 1.192 ± 0.037	1.012HisThr: 1.012 ± 0.035	1.216HisVal: 1.216 ± 0.037	0.277HisTrp: 0.277 ± 0.02	0.954HisTyr: 0.954 ± 0.04	0.0HisXaa: 0.0 ± 0.0
Ile	5.074IleAla: 5.074 ± 0.079	1.018IleCys: 1.018 ± 0.035	4.418IleAsp: 4.418 ± 0.074	4.309IleGlu: 4.309 ± 0.074	2.832IlePhe: 2.832 ± 0.072	4.542IleGly: 4.542 ± 0.096	1.377IleHis: 1.377 ± 0.038	4.506IleIle: 4.506 ± 0.097	4.347IleLys: 4.347 ± 0.078	5.581IleLeu: 5.581 ± 0.1	1.651IleMet: 1.651 ± 0.048	3.289IleAsn: 3.289 ± 0.057	2.849IlePro: 2.849 ± 0.058	2.26IleGln: 2.26 ± 0.056	3.17IleArg: 3.17 ± 0.065	4.47IleSer: 4.47 ± 0.085	3.846IleThr: 3.846 ± 0.066	4.276IleVal: 4.276 ± 0.077	0.661IleTrp: 0.661 ± 0.026	2.637IleTyr: 2.637 ± 0.054	0.0IleXaa: 0.0 ± 0.0
Lys	5.538LysAla: 5.538 ± 0.079	0.652LysCys: 0.652 ± 0.028	4.623LysAsp: 4.623 ± 0.083	5.84LysGlu: 5.84 ± 0.103	2.52LysPhe: 2.52 ± 0.059	4.891LysGly: 4.891 ± 0.081	1.441LysHis: 1.441 ± 0.044	4.307LysIle: 4.307 ± 0.077	6.123LysLys: 6.123 ± 0.102	5.773LysLeu: 5.773 ± 0.087	2.729LysMet: 2.729 ± 0.058	3.915LysAsn: 3.915 ± 0.077	2.283LysPro: 2.283 ± 0.057	3.038LysGln: 3.038 ± 0.071	3.261LysArg: 3.261 ± 0.07	3.791LysSer: 3.791 ± 0.065	3.843LysThr: 3.843 ± 0.072	4.855LysVal: 4.855 ± 0.09	0.893LysTrp: 0.893 ± 0.037	3.224LysTyr: 3.224 ± 0.061	0.0LysXaa: 0.0 ± 0.0
Leu	6.601LeuAla: 6.601 ± 0.102	1.352LeuCys: 1.352 ± 0.041	4.852LeuAsp: 4.852 ± 0.082	4.864LeuGlu: 4.864 ± 0.097	3.931LeuPhe: 3.931 ± 0.072	5.793LeuGly: 5.793 ± 0.1	1.867LeuHis: 1.867 ± 0.049	5.058LeuIle: 5.058 ± 0.1	6.563LeuLys: 6.563 ± 0.105	8.047LeuLeu: 8.047 ± 0.132	2.81LeuMet: 2.81 ± 0.06	4.504LeuAsn: 4.504 ± 0.073	3.716LeuPro: 3.716 ± 0.072	3.528LeuGln: 3.528 ± 0.067	3.997LeuArg: 3.997 ± 0.077	6.14LeuSer: 6.14 ± 0.086	4.718LeuThr: 4.718 ± 0.084	5.273LeuVal: 5.273 ± 0.099	0.959LeuTrp: 0.959 ± 0.039	3.568LeuTyr: 3.568 ± 0.067	0.0LeuXaa: 0.0 ± 0.0
Met	2.553MetAla: 2.553 ± 0.06	0.262MetCys: 0.262 ± 0.017	1.655MetAsp: 1.655 ± 0.046	2.039MetGlu: 2.039 ± 0.048	1.058MetPhe: 1.058 ± 0.035	2.117MetGly: 2.117 ± 0.051	0.54MetHis: 0.54 ± 0.022	1.831MetIle: 1.831 ± 0.045	2.797MetLys: 2.797 ± 0.056	2.77MetLeu: 2.77 ± 0.066	1.224MetMet: 1.224 ± 0.042	1.673MetAsn: 1.673 ± 0.045	1.275MetPro: 1.275 ± 0.045	1.277MetGln: 1.277 ± 0.045	1.371MetArg: 1.371 ± 0.038	1.718MetSer: 1.718 ± 0.053	1.654MetThr: 1.654 ± 0.043	1.944MetVal: 1.944 ± 0.045	0.235MetTrp: 0.235 ± 0.017	0.846MetTyr: 0.846 ± 0.032	0.0MetXaa: 0.0 ± 0.0
Asn	3.575AsnAla: 3.575 ± 0.068	0.538AsnCys: 0.538 ± 0.027	2.733AsnAsp: 2.733 ± 0.059	3.009AsnGlu: 3.009 ± 0.065	2.284AsnPhe: 2.284 ± 0.051	3.76AsnGly: 3.76 ± 0.08	1.145AsnHis: 1.145 ± 0.04	3.762AsnIle: 3.762 ± 0.075	3.363AsnLys: 3.363 ± 0.071	4.512AsnLeu: 4.512 ± 0.071	1.372AsnMet: 1.372 ± 0.038	2.608AsnAsn: 2.608 ± 0.064	2.343AsnPro: 2.343 ± 0.055	1.844AsnGln: 1.844 ± 0.053	2.258AsnArg: 2.258 ± 0.053	2.788AsnSer: 2.788 ± 0.07	2.531AsnThr: 2.531 ± 0.048	3.308AsnVal: 3.308 ± 0.063	0.693AsnTrp: 0.693 ± 0.032	2.322AsnTyr: 2.322 ± 0.059	0.0AsnXaa: 0.0 ± 0.0
Pro	2.496ProAla: 2.496 ± 0.065	0.412ProCys: 0.412 ± 0.023	2.229ProAsp: 2.229 ± 0.057	2.936ProGlu: 2.936 ± 0.069	1.703ProPhe: 1.703 ± 0.04	2.195ProGly: 2.195 ± 0.049	0.759ProHis: 0.759 ± 0.033	2.17ProIle: 2.17 ± 0.046	2.224ProLys: 2.224 ± 0.058	2.822ProLeu: 2.822 ± 0.061	1.062ProMet: 1.062 ± 0.034	1.608ProAsn: 1.608 ± 0.048	0.658ProPro: 0.658 ± 0.029	1.376ProGln: 1.376 ± 0.039	1.272ProArg: 1.272 ± 0.04	2.147ProSer: 2.147 ± 0.058	1.991ProThr: 1.991 ± 0.047	2.493ProVal: 2.493 ± 0.052	0.49ProTrp: 0.49 ± 0.024	1.635ProTyr: 1.635 ± 0.043	0.0ProXaa: 0.0 ± 0.0
Gln	2.721GlnAla: 2.721 ± 0.062	0.343GlnCys: 0.343 ± 0.019	1.808GlnAsp: 1.808 ± 0.041	2.504GlnGlu: 2.504 ± 0.057	1.364GlnPhe: 1.364 ± 0.05	2.313GlnGly: 2.313 ± 0.053	0.788GlnHis: 0.788 ± 0.031	2.491GlnIle: 2.491 ± 0.059	3.017GlnLys: 3.017 ± 0.071	3.73GlnLeu: 3.73 ± 0.077	1.306GlnMet: 1.306 ± 0.046	1.857GlnAsn: 1.857 ± 0.05	1.31GlnPro: 1.31 ± 0.04	1.992GlnGln: 1.992 ± 0.056	1.66GlnArg: 1.66 ± 0.048	1.945GlnSer: 1.945 ± 0.048	1.962GlnThr: 1.962 ± 0.046	2.342GlnVal: 2.342 ± 0.048	0.437GlnTrp: 0.437 ± 0.022	1.55GlnTyr: 1.55 ± 0.044	0.0GlnXaa: 0.0 ± 0.0
Arg	2.534ArgAla: 2.534 ± 0.062	0.55ArgCys: 0.55 ± 0.026	2.195ArgAsp: 2.195 ± 0.05	2.848ArgGlu: 2.848 ± 0.064	2.091ArgPhe: 2.091 ± 0.057	2.445ArgGly: 2.445 ± 0.058	1.017ArgHis: 1.017 ± 0.036	3.306ArgIle: 3.306 ± 0.065	3.661ArgLys: 3.661 ± 0.078	4.173ArgLeu: 4.173 ± 0.071	1.614ArgMet: 1.614 ± 0.04	2.542ArgAsn: 2.542 ± 0.054	1.489ArgPro: 1.489 ± 0.046	1.976ArgGln: 1.976 ± 0.051	2.188ArgArg: 2.188 ± 0.064	2.239ArgSer: 2.239 ± 0.061	2.079ArgThr: 2.079 ± 0.058	2.476ArgVal: 2.476 ± 0.063	0.581ArgTrp: 0.581 ± 0.024	2.065ArgTyr: 2.065 ± 0.053	0.0ArgXaa: 0.0 ± 0.0
Ser	4.16SerAla: 4.16 ± 0.072	0.888SerCys: 0.888 ± 0.031	3.516SerAsp: 3.516 ± 0.068	3.472SerGlu: 3.472 ± 0.073	3.064SerPhe: 3.064 ± 0.076	4.037SerGly: 4.037 ± 0.076	1.276SerHis: 1.276 ± 0.04	4.201SerIle: 4.201 ± 0.076	3.927SerLys: 3.927 ± 0.078	5.75SerLeu: 5.75 ± 0.092	1.62SerMet: 1.62 ± 0.051	2.765SerAsn: 2.765 ± 0.053	2.046SerPro: 2.046 ± 0.047	2.121SerGln: 2.121 ± 0.055	2.496SerArg: 2.496 ± 0.059	3.873SerSer: 3.873 ± 0.101	3.034SerThr: 3.034 ± 0.061	3.847SerVal: 3.847 ± 0.057	0.784SerTrp: 0.784 ± 0.035	2.721SerTyr: 2.721 ± 0.059	0.0SerXaa: 0.0 ± 0.0
Thr	3.827ThrAla: 3.827 ± 0.067	0.592ThrCys: 0.592 ± 0.025	3.092ThrAsp: 3.092 ± 0.067	3.055ThrGlu: 3.055 ± 0.063	2.454ThrPhe: 2.454 ± 0.064	3.795ThrGly: 3.795 ± 0.066	1.145ThrHis: 1.145 ± 0.039	3.832ThrIle: 3.832 ± 0.078	3.046ThrLys: 3.046 ± 0.067	5.085ThrLeu: 5.085 ± 0.076	1.427ThrMet: 1.427 ± 0.04	2.304ThrAsn: 2.304 ± 0.054	2.534ThrPro: 2.534 ± 0.057	1.792ThrGln: 1.792 ± 0.045	2.102ThrArg: 2.102 ± 0.05	3.275ThrSer: 3.275 ± 0.069	2.989ThrThr: 2.989 ± 0.069	3.403ThrVal: 3.403 ± 0.075	0.637ThrTrp: 0.637 ± 0.029	2.283ThrTyr: 2.283 ± 0.051	0.0ThrXaa: 0.0 ± 0.0
Val	4.883ValAla: 4.883 ± 0.089	0.953ValCys: 0.953 ± 0.033	3.966ValAsp: 3.966 ± 0.07	4.229ValGlu: 4.229 ± 0.071	2.674ValPhe: 2.674 ± 0.061	4.084ValGly: 4.084 ± 0.083	1.089ValHis: 1.089 ± 0.037	4.196ValIle: 4.196 ± 0.08	4.792ValLys: 4.792 ± 0.081	5.541ValLeu: 5.541 ± 0.102	1.886ValMet: 1.886 ± 0.048	3.323ValAsn: 3.323 ± 0.061	2.246ValPro: 2.246 ± 0.056	1.887ValGln: 1.887 ± 0.043	2.702ValArg: 2.702 ± 0.056	4.264ValSer: 4.264 ± 0.075	3.3ValThr: 3.3 ± 0.069	4.526ValVal: 4.526 ± 0.077	0.699ValTrp: 0.699 ± 0.029	2.364ValTyr: 2.364 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	0.833TrpAla: 0.833 ± 0.033	0.187TrpCys: 0.187 ± 0.013	0.685TrpAsp: 0.685 ± 0.033	0.769TrpGlu: 0.769 ± 0.033	0.575TrpPhe: 0.575 ± 0.029	0.909TrpGly: 0.909 ± 0.037	0.306TrpHis: 0.306 ± 0.021	0.769TrpIle: 0.769 ± 0.028	1.006TrpLys: 1.006 ± 0.037	1.199TrpLeu: 1.199 ± 0.038	0.469TrpMet: 0.469 ± 0.022	0.759TrpAsn: 0.759 ± 0.033	0.265TrpPro: 0.265 ± 0.02	0.564TrpGln: 0.564 ± 0.026	0.574TrpArg: 0.574 ± 0.031	0.68TrpSer: 0.68 ± 0.03	0.603TrpThr: 0.603 ± 0.028	0.685TrpVal: 0.685 ± 0.033	0.195TrpTrp: 0.195 ± 0.017	0.493TrpTyr: 0.493 ± 0.026	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.938TyrAla: 2.938 ± 0.055	0.587TyrCys: 0.587 ± 0.027	2.701TyrAsp: 2.701 ± 0.057	2.349TyrGlu: 2.349 ± 0.053	2.008TyrPhe: 2.008 ± 0.046	2.909TyrGly: 2.909 ± 0.064	1.052TyrHis: 1.052 ± 0.036	2.807TyrIle: 2.807 ± 0.067	2.731TyrLys: 2.731 ± 0.058	3.957TyrLeu: 3.957 ± 0.07	1.14TyrMet: 1.14 ± 0.039	2.334TyrAsn: 2.334 ± 0.056	1.691TyrPro: 1.691 ± 0.05	1.843TyrGln: 1.843 ± 0.051	2.247TyrArg: 2.247 ± 0.059	2.546TyrSer: 2.546 ± 0.063	2.343TyrThr: 2.343 ± 0.057	2.513TyrVal: 2.513 ± 0.05	0.567TyrTrp: 0.567 ± 0.027	2.167TyrTyr: 2.167 ± 0.061	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2572 proteins (830833 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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