Streptococcus phage Javan570
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6BAD1|A0A4D6BAD1_9CAUD Capsid and scaffold protein OS=Streptococcus phage Javan570 OX=2548265 GN=Javan570_0039 PE=4 SV=1
MM1 pKa = 7.34 QSTGLLDD8 pKa = 3.77 VNGKK12 pKa = 9.82 EE13 pKa = 3.88 IFEE16 pKa = 4.42 GDD18 pKa = 3.71 VVFVRR23 pKa = 11.84 DD24 pKa = 3.6 EE25 pKa = 4.64 LIEE28 pKa = 4.38 FGPGVYY34 pKa = 9.57 EE35 pKa = 3.86 VVYY38 pKa = 10.85 SQDD41 pKa = 2.87 HH42 pKa = 6.44 LGWMFYY48 pKa = 11.0 DD49 pKa = 4.05 KK50 pKa = 10.7 EE51 pKa = 4.15 NHH53 pKa = 6.38 DD54 pKa = 3.92 FCFISTCTWDD64 pKa = 3.41 EE65 pKa = 4.11 LEE67 pKa = 5.01 IIGNAHH73 pKa = 5.73 QNPEE77 pKa = 4.14 LVEE80 pKa = 4.33 DD81 pKa = 4.7 LEE83 pKa = 4.46
Molecular weight: 9.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.76
IPC2_protein 3.91
IPC_protein 3.821
Toseland 3.643
ProMoST 3.935
Dawson 3.795
Bjellqvist 4.012
Wikipedia 3.706
Rodwell 3.668
Grimsley 3.567
Solomon 3.783
Lehninger 3.732
Nozaki 3.923
DTASelect 4.075
Thurlkill 3.694
EMBOSS 3.719
Sillero 3.948
Patrickios 0.846
IPC_peptide 3.783
IPC2_peptide 3.923
IPC2.peptide.svr19 3.866
Protein with the highest isoelectric point:
>tr|A0A4D6BC03|A0A4D6BC03_9CAUD Uncharacterized protein OS=Streptococcus phage Javan570 OX=2548265 GN=Javan570_0012 PE=4 SV=1
MM1 pKa = 7.88 NYY3 pKa = 10.14 RR4 pKa = 11.84 KK5 pKa = 9.82 LDD7 pKa = 3.86 EE8 pKa = 4.24 QEE10 pKa = 4.5 HH11 pKa = 5.66 LAEE14 pKa = 4.39 LALNLRR20 pKa = 11.84 YY21 pKa = 8.01 TLNAKK26 pKa = 9.62 KK27 pKa = 10.52 VSPNKK32 pKa = 10.09 LSKK35 pKa = 10.41 KK36 pKa = 8.11 KK37 pKa = 10.28 QKK39 pKa = 10.64 EE40 pKa = 4.07 KK41 pKa = 10.41 VRR43 pKa = 11.84 KK44 pKa = 8.94 AFNVSMQLKK53 pKa = 9.07 QNKK56 pKa = 6.67 PTDD59 pKa = 3.3 MLARR63 pKa = 11.84 IEE65 pKa = 4.13 KK66 pKa = 10.47 LNAHH70 pKa = 6.2 FRR72 pKa = 11.84 NRR74 pKa = 3.26
Molecular weight: 8.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.179
IPC2_protein 9.692
IPC_protein 9.78
Toseland 10.774
ProMoST 10.379
Dawson 10.833
Bjellqvist 10.423
Wikipedia 10.95
Rodwell 11.462
Grimsley 10.862
Solomon 10.877
Lehninger 10.877
Nozaki 10.73
DTASelect 10.423
Thurlkill 10.745
EMBOSS 11.14
Sillero 10.745
Patrickios 11.199
IPC_peptide 10.891
IPC2_peptide 8.785
IPC2.peptide.svr19 8.733
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
56
0
56
11397
38
2017
203.5
22.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.563 ± 0.847
0.483 ± 0.118
5.923 ± 0.433
7.73 ± 0.728
3.703 ± 0.162
6.107 ± 0.491
1.228 ± 0.166
6.396 ± 0.248
7.748 ± 0.566
8.265 ± 0.355
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.343 ± 0.361
5.896 ± 0.374
2.457 ± 0.212
4.721 ± 0.242
4.606 ± 0.565
6.756 ± 0.716
6.528 ± 0.661
6.809 ± 0.255
1.035 ± 0.116
3.703 ± 0.419
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here