Gordonia phage Gibbles

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Orchidvirus; unclassified Orchidvirus

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 117 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514TZE8|A0A514TZE8_9CAUD Uncharacterized protein OS=Gordonia phage Gibbles OX=2593334 GN=71 PE=4 SV=1
MM1 pKa = 7.12YY2 pKa = 10.65ALTVYY7 pKa = 10.74CDD9 pKa = 3.95DD10 pKa = 4.38GNGKK14 pKa = 9.67YY15 pKa = 9.9VDD17 pKa = 4.35PMSGTYY23 pKa = 9.45SVEE26 pKa = 4.35RR27 pKa = 11.84IEE29 pKa = 5.53SMITEE34 pKa = 4.44LLISVATDD42 pKa = 3.0NVVSFEE48 pKa = 3.88ISKK51 pKa = 10.8SGEE54 pKa = 3.74

Molecular weight: 5.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514TZ93|A0A514TZ93_9CAUD Uncharacterized protein OS=Gordonia phage Gibbles OX=2593334 GN=50 PE=4 SV=1
MM1 pKa = 7.58QDD3 pKa = 2.87GTVHH7 pKa = 5.68MRR9 pKa = 11.84CRR11 pKa = 11.84DD12 pKa = 3.48CFRR15 pKa = 11.84FTKK18 pKa = 10.31ISFVNRR24 pKa = 11.84DD25 pKa = 3.69NIKK28 pKa = 8.61ATHH31 pKa = 5.99GVRR34 pKa = 11.84PPTALAGG41 pKa = 3.75

Molecular weight: 4.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

117

0

117

24484

41

2914

209.3

23.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.683 ± 0.749

0.972 ± 0.144

6.461 ± 0.25

6.245 ± 0.303

3.823 ± 0.117

7.319 ± 0.351

1.773 ± 0.158

6.175 ± 0.152

5.992 ± 0.298

7.086 ± 0.211

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.745 ± 0.086

5.248 ± 0.108

5.011 ± 0.298

3.496 ± 0.152

4.791 ± 0.201

6.253 ± 0.181

6.421 ± 0.275

6.539 ± 0.25

1.548 ± 0.118

3.419 ± 0.239
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski