Mengla dianlovirus
Average proteome isoelectric point is 7.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3Q8U6Q5|A0A3Q8U6Q5_9MONO Matrix protein VP40 OS=Mengla dianlovirus OX=2496529 GN=VP40 PE=3 SV=1
MM1 pKa = 8.02 DD2 pKa = 4.37 LHH4 pKa = 6.96 GLLEE8 pKa = 4.89 LGTRR12 pKa = 11.84 PTAPHH17 pKa = 4.84 VRR19 pKa = 11.84 SRR21 pKa = 11.84 KK22 pKa = 8.5 IVIYY26 pKa = 8.3 EE27 pKa = 3.97 TGNQIIICNQIIDD40 pKa = 4.07 AVSAGIDD47 pKa = 3.76 LGDD50 pKa = 3.98 LLEE53 pKa = 4.96 GCLLTLCLEE62 pKa = 4.65 HH63 pKa = 7.61 YY64 pKa = 10.19 YY65 pKa = 11.2 GSDD68 pKa = 3.04 KK69 pKa = 11.6 DD70 pKa = 4.13 KK71 pKa = 11.3 FNSSQMAAYY80 pKa = 10.18 LRR82 pKa = 11.84 DD83 pKa = 3.25 AGYY86 pKa = 10.78 DD87 pKa = 3.44 FEE89 pKa = 5.48 VIRR92 pKa = 11.84 AQDD95 pKa = 3.4 AKK97 pKa = 10.87 KK98 pKa = 10.39 LADD101 pKa = 5.04 LIPRR105 pKa = 11.84 EE106 pKa = 4.14 SHH108 pKa = 6.13 LLNVISALEE117 pKa = 3.95 NLDD120 pKa = 3.5 GSEE123 pKa = 4.15 KK124 pKa = 10.26 NKK126 pKa = 9.95 QRR128 pKa = 11.84 VGLFLSFCSLFLPKK142 pKa = 10.43 LVVGDD147 pKa = 3.63 KK148 pKa = 11.22 ASIEE152 pKa = 3.94 KK153 pKa = 10.52 ALRR156 pKa = 11.84 QVAIHH161 pKa = 5.78 QEE163 pKa = 3.96 QGMVVYY169 pKa = 10.01 PPTWLTTGFMKK180 pKa = 11.06 LIFSIVRR187 pKa = 11.84 ASFIVKK193 pKa = 9.59 FVLIHH198 pKa = 5.68 QGINLVTGHH207 pKa = 7.28 DD208 pKa = 3.75 AYY210 pKa = 11.29 DD211 pKa = 3.64 NVISNSINQTRR222 pKa = 11.84 FSGLLIVKK230 pKa = 8.18 TVLEE234 pKa = 5.12 HH235 pKa = 6.31 ILQRR239 pKa = 11.84 TEE241 pKa = 3.61 NGVILHH247 pKa = 6.07 PLVRR251 pKa = 11.84 TSKK254 pKa = 10.25 VKK256 pKa = 10.96 SEE258 pKa = 4.06 VEE260 pKa = 4.11 SFKK263 pKa = 11.08 VALRR267 pKa = 11.84 GLARR271 pKa = 11.84 HH272 pKa = 6.19 KK273 pKa = 10.44 EE274 pKa = 4.0 YY275 pKa = 11.22 APFARR280 pKa = 11.84 VLNLSGVNNLEE291 pKa = 4.48 HH292 pKa = 7.06 GLFPQLSAIAIGVATAHH309 pKa = 6.61 GSTLAGVSVGEE320 pKa = 4.2 QYY322 pKa = 10.54 QQLRR326 pKa = 11.84 EE327 pKa = 4.08 AAHH330 pKa = 6.28 DD331 pKa = 4.19 AEE333 pKa = 5.43 LKK335 pKa = 9.0 LQRR338 pKa = 11.84 RR339 pKa = 11.84 RR340 pKa = 11.84 EE341 pKa = 3.91 QMEE344 pKa = 3.96 ISSLEE349 pKa = 3.97 LDD351 pKa = 4.17 LEE353 pKa = 4.28 EE354 pKa = 5.19 QKK356 pKa = 10.75 ILEE359 pKa = 4.18 QFHH362 pKa = 4.9 QQKK365 pKa = 8.7 TEE367 pKa = 3.68 ITHH370 pKa = 5.13 TQTLAVLTQKK380 pKa = 10.61 KK381 pKa = 9.65 EE382 pKa = 4.07 KK383 pKa = 9.72 LAKK386 pKa = 10.29 LAGEE390 pKa = 4.58 LGADD394 pKa = 3.52 KK395 pKa = 10.74 LFQPVIPQPNDD406 pKa = 3.02 KK407 pKa = 10.33 PQMTTVIDD415 pKa = 3.95 PSRR418 pKa = 11.84 TSVKK422 pKa = 9.82 IQTSFLPPPSTKK434 pKa = 10.96 DD435 pKa = 3.24 NMEE438 pKa = 3.92 GQIPEE443 pKa = 4.35 SPEE446 pKa = 3.64 SSTISSSSSCIDD458 pKa = 3.45 PNDD461 pKa = 3.82 PFALLIDD468 pKa = 4.79 DD469 pKa = 5.5 DD470 pKa = 4.22 EE471 pKa = 5.13 QEE473 pKa = 4.99 GDD475 pKa = 3.76 FQSADD480 pKa = 3.49 EE481 pKa = 5.35 GDD483 pKa = 4.22 SQDD486 pKa = 3.66 QSAEE490 pKa = 3.66 AARR493 pKa = 11.84 QEE495 pKa = 4.5 EE496 pKa = 4.35 IRR498 pKa = 11.84 KK499 pKa = 9.83 AEE501 pKa = 4.1 QKK503 pKa = 10.77 FKK505 pKa = 10.85 GLPRR509 pKa = 11.84 TIPTKK514 pKa = 10.51 DD515 pKa = 3.21 QNPPIKK521 pKa = 9.89 PQRR524 pKa = 11.84 TNLAPVQEE532 pKa = 4.52 EE533 pKa = 4.58 SEE535 pKa = 4.43 SEE537 pKa = 4.17 YY538 pKa = 8.68 TTTSDD543 pKa = 3.12 EE544 pKa = 4.43 SGSEE548 pKa = 4.32 DD549 pKa = 3.58 EE550 pKa = 6.64 SSDD553 pKa = 3.27 QRR555 pKa = 11.84 NIEE558 pKa = 4.21 VPPPPLYY565 pKa = 9.69 DD566 pKa = 3.26 TSTSEE571 pKa = 4.06 IGDD574 pKa = 3.43 MGAAEE579 pKa = 4.29 HH580 pKa = 6.2 TLDD583 pKa = 4.65 PFGDD587 pKa = 4.02 MPPLEE592 pKa = 4.59 TDD594 pKa = 3.27 VLTPQDD600 pKa = 3.59 TATVPSAPPSSPTPSTNRR618 pKa = 11.84 QGEE621 pKa = 4.08 AQTQNGEE628 pKa = 4.54 DD629 pKa = 4.33 SSQDD633 pKa = 2.43 WPRR636 pKa = 11.84 RR637 pKa = 11.84 VKK639 pKa = 9.54 TNKK642 pKa = 9.17 GRR644 pKa = 11.84 EE645 pKa = 4.03 FMFPTDD651 pKa = 3.98 LLHH654 pKa = 6.23 RR655 pKa = 11.84 TPPQVLLDD663 pKa = 3.79 ALVNEE668 pKa = 4.66 YY669 pKa = 10.29 EE670 pKa = 4.56 SPLSATEE677 pKa = 5.73 LSDD680 pKa = 4.09 DD681 pKa = 3.6 WPEE684 pKa = 3.75 MTFEE688 pKa = 3.98 EE689 pKa = 4.57 RR690 pKa = 11.84 KK691 pKa = 10.11 NVAFNLL697 pKa = 3.9
Molecular weight: 77.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.903
IPC2_protein 4.851
IPC_protein 4.813
Toseland 4.711
ProMoST 4.927
Dawson 4.774
Bjellqvist 4.914
Wikipedia 4.635
Rodwell 4.685
Grimsley 4.622
Solomon 4.774
Lehninger 4.724
Nozaki 4.889
DTASelect 5.029
Thurlkill 4.698
EMBOSS 4.66
Sillero 4.952
Patrickios 4.19
IPC_peptide 4.787
IPC2_peptide 4.952
IPC2.peptide.svr19 4.901
Protein with the highest isoelectric point:
>tr|A0A3S8UVN5|A0A3S8UVN5_9MONO Minor nucleoprotein VP30 OS=Mengla dianlovirus OX=2496529 GN=VP30 PE=1 SV=1
MM1 pKa = 7.78 SEE3 pKa = 3.85 RR4 pKa = 11.84 QSRR7 pKa = 11.84 GRR9 pKa = 11.84 SRR11 pKa = 11.84 TRR13 pKa = 11.84 RR14 pKa = 11.84 QPDD17 pKa = 3.27 EE18 pKa = 4.29 VITRR22 pKa = 11.84 QEE24 pKa = 3.91 VFNPDD29 pKa = 3.17 QGPHH33 pKa = 5.53 GFHH36 pKa = 6.33 RR37 pKa = 11.84 RR38 pKa = 11.84 SRR40 pKa = 11.84 STSNIRR46 pKa = 11.84 SNYY49 pKa = 9.0 PGGNQYY55 pKa = 10.1 TNQIGGGRR63 pKa = 11.84 SNQFQPFLRR72 pKa = 11.84 PPPPPKK78 pKa = 10.27 DD79 pKa = 3.17 LCKK82 pKa = 10.33 SFKK85 pKa = 10.84 VGLPCNDD92 pKa = 3.51 KK93 pKa = 11.35 GCTKK97 pKa = 10.93 DD98 pKa = 4.02 HH99 pKa = 7.15 DD100 pKa = 4.81 LSNLTNRR107 pKa = 11.84 EE108 pKa = 4.01 LLILIAKK115 pKa = 7.63 RR116 pKa = 11.84 TFPEE120 pKa = 4.08 YY121 pKa = 10.79 QKK123 pKa = 10.75 IPDD126 pKa = 3.58 NTGSKK131 pKa = 10.8 GSADD135 pKa = 3.84 LSKK138 pKa = 11.13 GIPDD142 pKa = 3.69 TSNAQNRR149 pKa = 11.84 DD150 pKa = 2.88 ILTLEE155 pKa = 3.96 NLGDD159 pKa = 3.31 ILKK162 pKa = 9.95 YY163 pKa = 10.84 LNSADD168 pKa = 3.94 LTTLDD173 pKa = 4.11 EE174 pKa = 4.49 VSMRR178 pKa = 11.84 AALSLTCAGIRR189 pKa = 11.84 KK190 pKa = 7.57 TSRR193 pKa = 11.84 SMINTLTEE201 pKa = 3.78 QHH203 pKa = 6.01 VSAEE207 pKa = 4.13 NLSPDD212 pKa = 2.96 QTQIIKK218 pKa = 8.09 QTYY221 pKa = 8.21 TGIHH225 pKa = 6.66 LDD227 pKa = 3.03 KK228 pKa = 11.2 GGNFEE233 pKa = 4.4 AALWKK238 pKa = 10.51 NWDD241 pKa = 3.57 RR242 pKa = 11.84 RR243 pKa = 11.84 SISLFLQAAISVLNTTPCEE262 pKa = 4.04 SSKK265 pKa = 11.37 SVISAYY271 pKa = 10.2 NHH273 pKa = 6.44 FLQKK277 pKa = 10.58 PGDD280 pKa = 3.72 IKK282 pKa = 10.7 RR283 pKa = 11.84 TTLGSINSSVV293 pKa = 2.86
Molecular weight: 32.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.09
IPC2_protein 8.829
IPC_protein 8.77
Toseland 9.589
ProMoST 9.297
Dawson 9.823
Bjellqvist 9.545
Wikipedia 9.97
Rodwell 10.175
Grimsley 9.867
Solomon 9.867
Lehninger 9.838
Nozaki 9.75
DTASelect 9.487
Thurlkill 9.692
EMBOSS 10.014
Sillero 9.78
Patrickios 7.629
IPC_peptide 9.867
IPC2_peptide 8.258
IPC2.peptide.svr19 7.86
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
4814
253
2276
687.7
77.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.629 ± 0.501
1.329 ± 0.231
4.965 ± 0.504
5.526 ± 0.646
4.3 ± 0.416
5.318 ± 0.473
2.327 ± 0.173
7.208 ± 0.372
6.024 ± 0.351
10.656 ± 0.457
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.87 ± 0.213
5.526 ± 0.377
5.193 ± 0.593
5.006 ± 0.479
4.882 ± 0.245
8.288 ± 0.329
6.398 ± 0.479
4.965 ± 0.479
1.122 ± 0.18
3.469 ± 0.672
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here