Bacteroidetes bacterium B1(2017)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; unclassified Bacteroidetes

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4556 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A257L9F5|A0A257L9F5_9BACT Uncharacterized protein OS=Bacteroidetes bacterium B1(2017) OX=2015582 GN=CFE21_10775 PE=4 SV=1
MM1 pKa = 7.73AIIITDD7 pKa = 3.35EE8 pKa = 4.75CINCGACEE16 pKa = 4.03PEE18 pKa = 4.51CPNNAIYY25 pKa = 10.14EE26 pKa = 4.44AGAEE30 pKa = 4.15WAWADD35 pKa = 3.6GNNLSGTISVDD46 pKa = 2.91GEE48 pKa = 4.41TIDD51 pKa = 3.35VSKK54 pKa = 11.04RR55 pKa = 11.84FAPKK59 pKa = 10.32SEE61 pKa = 3.67DD62 pKa = 3.4TYY64 pKa = 11.21FIVSSKK70 pKa = 9.67CTEE73 pKa = 3.6CVGFHH78 pKa = 6.74EE79 pKa = 5.25EE80 pKa = 4.07PQCAAVCPVDD90 pKa = 4.27CCVPDD95 pKa = 4.07PDD97 pKa = 4.49VVEE100 pKa = 4.27TQEE103 pKa = 4.78ALLEE107 pKa = 4.48KK108 pKa = 9.9KK109 pKa = 10.44NRR111 pKa = 11.84MHH113 pKa = 7.4II114 pKa = 3.81

Molecular weight: 12.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A257L0Q5|A0A257L0Q5_9BACT 2-amino-3-ketobutyrate coenzyme A ligase OS=Bacteroidetes bacterium B1(2017) OX=2015582 GN=kbl PE=3 SV=1
MM1 pKa = 7.85DD2 pKa = 4.17RR3 pKa = 11.84QATTEE8 pKa = 4.0RR9 pKa = 11.84TPAPNSGLAKK19 pKa = 10.45VAVQCSADD27 pKa = 3.58TFVVNQSLVLRR38 pKa = 11.84INICDD43 pKa = 3.67KK44 pKa = 10.8NRR46 pKa = 11.84HH47 pKa = 5.19LRR49 pKa = 11.84QAAKK53 pKa = 10.13RR54 pKa = 11.84YY55 pKa = 8.96RR56 pKa = 11.84QAIWKK61 pKa = 9.16YY62 pKa = 6.68KK63 pKa = 7.99TKK65 pKa = 10.66YY66 pKa = 9.03VSLRR70 pKa = 11.84KK71 pKa = 9.83KK72 pKa = 10.32YY73 pKa = 10.77VSS75 pKa = 3.22

Molecular weight: 8.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4556

0

4556

1641265

38

7871

360.2

40.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.666 ± 0.036

0.967 ± 0.013

4.851 ± 0.031

5.708 ± 0.063

5.314 ± 0.033

6.801 ± 0.047

1.685 ± 0.018

7.494 ± 0.032

7.295 ± 0.06

9.536 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.249 ± 0.019

6.312 ± 0.05

3.7 ± 0.022

3.534 ± 0.024

3.269 ± 0.026

7.158 ± 0.056

6.081 ± 0.084

6.029 ± 0.027

1.15 ± 0.012

4.2 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski