Conservatibacter flavescens
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2416 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2M8S431|A0A2M8S431_9PAST Glutamine--tRNA ligase OS=Conservatibacter flavescens OX=28161 GN=glnS PE=3 SV=1
MM1 pKa = 7.65 AIVGLFYY8 pKa = 11.01 GSDD11 pKa = 3.2 TGNTEE16 pKa = 4.47 NIAKK20 pKa = 9.24 MIQKK24 pKa = 9.56 QLGSDD29 pKa = 3.38 LVDD32 pKa = 3.09 IRR34 pKa = 11.84 DD35 pKa = 3.42 IAKK38 pKa = 10.28 SSKK41 pKa = 10.35 EE42 pKa = 3.95 DD43 pKa = 3.02 IEE45 pKa = 5.73 AYY47 pKa = 10.19 DD48 pKa = 4.65 FIMIGIPTWYY58 pKa = 10.03 YY59 pKa = 11.56 GEE61 pKa = 4.97 AQADD65 pKa = 3.42 WDD67 pKa = 4.41 DD68 pKa = 4.32 FFPTLEE74 pKa = 5.65 DD75 pKa = 3.64 IDD77 pKa = 4.18 FTDD80 pKa = 3.25 KK81 pKa = 10.9 LVAIFGCGDD90 pKa = 3.25 QEE92 pKa = 5.04 DD93 pKa = 4.3 YY94 pKa = 11.79 AEE96 pKa = 4.32 YY97 pKa = 10.5 FCDD100 pKa = 4.34 AMGTVRR106 pKa = 11.84 NIIEE110 pKa = 4.05 PHH112 pKa = 5.95 GATIVGYY119 pKa = 9.34 WPTEE123 pKa = 3.87 GYY125 pKa = 10.48 NFEE128 pKa = 4.12 VSQALVDD135 pKa = 3.81 EE136 pKa = 4.45 NTFVGLCIDD145 pKa = 4.19 EE146 pKa = 4.82 DD147 pKa = 3.95 RR148 pKa = 11.84 QPEE151 pKa = 4.37 LTSQRR156 pKa = 11.84 VEE158 pKa = 3.78 TWVKK162 pKa = 9.56 QVYY165 pKa = 10.2 EE166 pKa = 4.27 EE167 pKa = 4.11 MCLAEE172 pKa = 5.08 LADD175 pKa = 3.75
Molecular weight: 19.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.73
IPC2_protein 3.757
IPC_protein 3.745
Toseland 3.528
ProMoST 3.859
Dawson 3.732
Bjellqvist 3.91
Wikipedia 3.643
Rodwell 3.567
Grimsley 3.439
Solomon 3.719
Lehninger 3.668
Nozaki 3.846
DTASelect 4.05
Thurlkill 3.579
EMBOSS 3.656
Sillero 3.859
Patrickios 0.769
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.799
Protein with the highest isoelectric point:
>tr|A0A2M8S3G7|A0A2M8S3G7_9PAST Phosphate import ATP-binding protein PstB OS=Conservatibacter flavescens OX=28161 GN=pstB PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 SRR14 pKa = 11.84 THH16 pKa = 6.4 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.15 GRR39 pKa = 11.84 KK40 pKa = 8.87 SLSAA44 pKa = 3.86
Molecular weight: 5.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2416
0
2416
780385
30
4148
323.0
36.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.472 ± 0.056
1.022 ± 0.017
4.954 ± 0.038
6.073 ± 0.051
4.364 ± 0.04
6.786 ± 0.06
2.284 ± 0.025
7.17 ± 0.048
5.749 ± 0.047
10.582 ± 0.066
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.448 ± 0.028
4.739 ± 0.052
3.774 ± 0.032
4.981 ± 0.05
4.363 ± 0.039
5.826 ± 0.04
5.345 ± 0.05
6.601 ± 0.044
1.2 ± 0.018
3.268 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here