Thermosyntropha lipolytica DSM 11003
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2141 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M5MVR3|A0A1M5MVR3_9FIRM Uncharacterized protein OS=Thermosyntropha lipolytica DSM 11003 OX=1123382 GN=SAMN02745221_01037 PE=4 SV=1
MM1 pKa = 7.45 RR2 pKa = 11.84 VEE4 pKa = 4.62 KK5 pKa = 10.81 GLLYY9 pKa = 10.45 TEE11 pKa = 3.72 NHH13 pKa = 5.51 EE14 pKa = 4.22 WLKK17 pKa = 11.14 VEE19 pKa = 4.38 GDD21 pKa = 3.2 KK22 pKa = 11.12 AYY24 pKa = 9.91 IGITDD29 pKa = 3.69 YY30 pKa = 11.44 AQEE33 pKa = 4.1 HH34 pKa = 6.63 LGDD37 pKa = 3.53 IVFVEE42 pKa = 4.5 LPEE45 pKa = 4.43 VDD47 pKa = 4.49 AEE49 pKa = 4.04 FDD51 pKa = 3.57 ADD53 pKa = 3.56 EE54 pKa = 5.23 GIAVIEE60 pKa = 4.26 SVKK63 pKa = 10.61 AVSSVYY69 pKa = 10.4 TPVACTILEE78 pKa = 4.25 VNEE81 pKa = 4.14 EE82 pKa = 4.24 LEE84 pKa = 4.48 GAPEE88 pKa = 4.56 LLNEE92 pKa = 4.29 DD93 pKa = 5.43 CYY95 pKa = 11.88 ANWIALISIKK105 pKa = 11.08 DD106 pKa = 3.41 MDD108 pKa = 4.67 GLNKK112 pKa = 10.5 LMDD115 pKa = 4.22 AEE117 pKa = 4.53 AYY119 pKa = 8.49 EE120 pKa = 4.32 AFCAEE125 pKa = 4.15 QEE127 pKa = 4.32 EE128 pKa = 4.64
Molecular weight: 14.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.897
IPC_protein 3.821
Toseland 3.656
ProMoST 3.923
Dawson 3.783
Bjellqvist 3.973
Wikipedia 3.656
Rodwell 3.668
Grimsley 3.567
Solomon 3.77
Lehninger 3.719
Nozaki 3.897
DTASelect 4.024
Thurlkill 3.681
EMBOSS 3.668
Sillero 3.935
Patrickios 0.985
IPC_peptide 3.77
IPC2_peptide 3.923
IPC2.peptide.svr19 3.856
Protein with the highest isoelectric point:
>tr|A0A1M5S4X8|A0A1M5S4X8_9FIRM Transposase OS=Thermosyntropha lipolytica DSM 11003 OX=1123382 GN=SAMN02745221_02168 PE=4 SV=1
MM1 pKa = 7.65 DD2 pKa = 3.59 QYY4 pKa = 11.78 HH5 pKa = 7.71 LIRR8 pKa = 11.84 HH9 pKa = 6.43 LYY11 pKa = 9.15 LVEE14 pKa = 4.33 GMSQRR19 pKa = 11.84 AIAKK23 pKa = 9.45 QLGISRR29 pKa = 11.84 NTVRR33 pKa = 11.84 RR34 pKa = 11.84 YY35 pKa = 10.26 CKK37 pKa = 10.44 GEE39 pKa = 3.72 QVPWEE44 pKa = 4.1 RR45 pKa = 11.84 KK46 pKa = 6.67 PVKK49 pKa = 9.81 RR50 pKa = 11.84 APSVITPGEE59 pKa = 4.1 ALSLLPTWSSRR70 pKa = 11.84 VGQKK74 pKa = 8.85 SFRR77 pKa = 11.84 ILCC80 pKa = 4.12
Molecular weight: 9.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.339
IPC2_protein 9.736
IPC_protein 10.467
Toseland 10.599
ProMoST 10.277
Dawson 10.73
Bjellqvist 10.438
Wikipedia 10.921
Rodwell 10.994
Grimsley 10.774
Solomon 10.818
Lehninger 10.789
Nozaki 10.599
DTASelect 10.423
Thurlkill 10.613
EMBOSS 10.994
Sillero 10.657
Patrickios 10.76
IPC_peptide 10.818
IPC2_peptide 9.516
IPC2.peptide.svr19 8.465
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2141
0
2141
645175
39
2074
301.3
33.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.818 ± 0.057
1.095 ± 0.024
5.008 ± 0.04
8.191 ± 0.068
4.048 ± 0.039
7.315 ± 0.045
1.628 ± 0.023
8.163 ± 0.04
7.277 ± 0.05
10.01 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.692 ± 0.023
3.939 ± 0.034
3.839 ± 0.033
2.802 ± 0.027
5.159 ± 0.043
4.846 ± 0.034
4.31 ± 0.032
7.118 ± 0.043
0.945 ± 0.018
3.797 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here