ANME-2 cluster archaeon

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanosarcinales; unclassified Methanosarcinales; ANME-2 cluster; unclassified ANME-2 cluster

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3377 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2V3JK96|A0A2V3JK96_9EURY FmdE domain-containing protein OS=ANME-2 cluster archaeon OX=2056317 GN=C4B59_07205 PE=4 SV=1
MM1 pKa = 7.22TRR3 pKa = 11.84LPLVAAAVVILAFAGVGAASMPPVPYY29 pKa = 10.42FSQCDD34 pKa = 3.8PRR36 pKa = 11.84WGSDD40 pKa = 2.9KK41 pKa = 11.28LGGDD45 pKa = 3.44GQTIYY50 pKa = 11.09SQGCTLTSAAMVMAYY65 pKa = 10.3YY66 pKa = 10.62GVDD69 pKa = 3.48TDD71 pKa = 4.42PKK73 pKa = 10.19RR74 pKa = 11.84LNDD77 pKa = 4.35AIGRR81 pKa = 11.84AGYY84 pKa = 10.02DD85 pKa = 3.23EE86 pKa = 5.4NYY88 pKa = 8.33WIHH91 pKa = 6.42WSAVSDD97 pKa = 3.67ACHH100 pKa = 7.33DD101 pKa = 3.54EE102 pKa = 4.38TNQIEE107 pKa = 4.45YY108 pKa = 10.91SPGTVKK114 pKa = 10.68PFDD117 pKa = 3.61TTVLNTHH124 pKa = 7.2LDD126 pKa = 3.35AGHH129 pKa = 6.42PVIVNVGGHH138 pKa = 5.18FVVVTGRR145 pKa = 11.84SDD147 pKa = 2.94GTYY150 pKa = 10.24YY151 pKa = 10.36INDD154 pKa = 5.12PISSAKK160 pKa = 8.97STLDD164 pKa = 3.21AYY166 pKa = 9.58PNRR169 pKa = 11.84VGMHH173 pKa = 6.74IYY175 pKa = 10.32SGNPPDD181 pKa = 3.54INKK184 pKa = 9.58PLVGDD189 pKa = 3.64WNGNGTDD196 pKa = 3.15TTGIYY201 pKa = 10.54NYY203 pKa = 7.5TTANFSLDD211 pKa = 3.22SGLNISFSISGDD223 pKa = 3.27IPITGDD229 pKa = 2.97WNGNGYY235 pKa = 8.17DD236 pKa = 3.69TIGVFRR242 pKa = 11.84PSTAQFFLDD251 pKa = 3.74YY252 pKa = 11.22DD253 pKa = 3.85NDD255 pKa = 4.18GVSDD259 pKa = 3.6MNATFGVIGDD269 pKa = 3.92IPVVGDD275 pKa = 3.27WDD277 pKa = 3.98GDD279 pKa = 3.67GGDD282 pKa = 3.74NIGVFRR288 pKa = 11.84QNHH291 pKa = 5.82SDD293 pKa = 3.16SGLTMFFLDD302 pKa = 4.74LDD304 pKa = 4.07NSGGSADD311 pKa = 3.45QSVAFGEE318 pKa = 4.37PDD320 pKa = 3.95DD321 pKa = 5.42LPVIGDD327 pKa = 3.49WDD329 pKa = 3.94GDD331 pKa = 3.63GDD333 pKa = 5.03DD334 pKa = 5.29NIGLYY339 pKa = 10.36RR340 pKa = 11.84PGSTTGVFYY349 pKa = 11.25LDD351 pKa = 3.69IDD353 pKa = 4.13NDD355 pKa = 3.63GGAADD360 pKa = 4.22IVTPEE365 pKa = 4.1YY366 pKa = 11.15GDD368 pKa = 4.67LGDD371 pKa = 5.1IPIAGDD377 pKa = 3.29WDD379 pKa = 3.9GDD381 pKa = 3.71SDD383 pKa = 5.38DD384 pKa = 5.04NIGVYY389 pKa = 10.07RR390 pKa = 11.84PGTGEE395 pKa = 3.92FFLNASMPSVPKK407 pKa = 10.06TIYY410 pKa = 10.76VDD412 pKa = 5.16DD413 pKa = 5.42DD414 pKa = 4.56FSDD417 pKa = 4.47DD418 pKa = 3.9PSEE421 pKa = 4.72HH422 pKa = 6.54KK423 pKa = 10.02WDD425 pKa = 4.46TIQEE429 pKa = 4.41GVDD432 pKa = 3.51DD433 pKa = 4.58ATDD436 pKa = 3.69GDD438 pKa = 4.52TVIVYY443 pKa = 10.28AGEE446 pKa = 4.04YY447 pKa = 10.07NEE449 pKa = 4.37TVGVGVEE456 pKa = 4.02NSITLWGAGVDD467 pKa = 3.94VVTVRR472 pKa = 11.84SKK474 pKa = 9.8CTWGRR479 pKa = 11.84VFDD482 pKa = 3.79VTAGYY487 pKa = 7.6TRR489 pKa = 11.84HH490 pKa = 4.94RR491 pKa = 11.84HH492 pKa = 4.83KK493 pKa = 11.12LRR495 pKa = 11.84FFYY498 pKa = 10.63RR499 pKa = 3.45

Molecular weight: 53.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2V3JI79|A0A2V3JI79_9EURY Uncharacterized protein OS=ANME-2 cluster archaeon OX=2056317 GN=C4B59_11875 PE=4 SV=1
MM1 pKa = 7.21GQIEE5 pKa = 4.73VQNLPQPEE13 pKa = 4.09MVLTYY18 pKa = 10.35RR19 pKa = 11.84AVFHH23 pKa = 6.63CMHH26 pKa = 7.03GGKK29 pKa = 9.5SVNVNSSNTPLKK41 pKa = 11.14GEE43 pKa = 3.87ILTNIRR49 pKa = 11.84PKK51 pKa = 11.11LNWTDD56 pKa = 3.5INWVKK61 pKa = 10.76VEE63 pKa = 3.95EE64 pKa = 4.27HH65 pKa = 6.61ANRR68 pKa = 11.84LQIRR72 pKa = 11.84ITMAVKK78 pKa = 9.83TEE80 pKa = 4.01INSCPGTEE88 pKa = 3.51RR89 pKa = 11.84VTRR92 pKa = 11.84CLTAKK97 pKa = 9.04QRR99 pKa = 11.84RR100 pKa = 11.84RR101 pKa = 11.84QRR103 pKa = 11.84YY104 pKa = 7.65EE105 pKa = 3.71GKK107 pKa = 10.46LSCTVLRR114 pKa = 11.84RR115 pKa = 11.84GEE117 pKa = 4.01AGNRR121 pKa = 11.84LVLSRR126 pKa = 11.84RR127 pKa = 11.84LKK129 pKa = 10.02MLL131 pKa = 3.86

Molecular weight: 15.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3377

0

3377

955486

22

3892

282.9

31.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.584 ± 0.058

1.423 ± 0.023

6.705 ± 0.045

6.985 ± 0.043

3.672 ± 0.033

7.716 ± 0.047

2.06 ± 0.021

7.707 ± 0.045

5.069 ± 0.046

8.621 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.632 ± 0.025

3.988 ± 0.066

4.044 ± 0.028

2.342 ± 0.023

5.331 ± 0.045

6.232 ± 0.036

5.875 ± 0.047

7.384 ± 0.041

1.144 ± 0.02

3.485 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski