Streptomyces sp. Z022

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6390 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G2JG24|A0A3G2JG24_9ACTN Uncharacterized protein OS=Streptomyces sp. Z022 OX=1442032 GN=D9753_22705 PE=4 SV=1
MM1 pKa = 7.97RR2 pKa = 11.84KK3 pKa = 8.92LQTVVIAAVAAAGGLSAIGAGTAVADD29 pKa = 4.25TSDD32 pKa = 3.52GGQQVSNLYY41 pKa = 10.15RR42 pKa = 11.84PYY44 pKa = 10.43QEE46 pKa = 5.76CSPQTAFEE54 pKa = 3.99NDD56 pKa = 2.68IPIAVLGLAGNLDD69 pKa = 3.92TTCGQFNDD77 pKa = 4.16AFSGG81 pKa = 3.66

Molecular weight: 8.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G2JDT3|A0A3G2JDT3_9ACTN Prephenate dehydratase OS=Streptomyces sp. Z022 OX=1442032 GN=pheA PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.89GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6390

0

6390

2106575

18

12001

329.7

35.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.676 ± 0.047

0.803 ± 0.011

5.974 ± 0.026

5.555 ± 0.031

2.679 ± 0.018

9.606 ± 0.038

2.436 ± 0.016

2.942 ± 0.024

1.976 ± 0.023

10.389 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.666 ± 0.014

1.679 ± 0.017

6.254 ± 0.032

2.707 ± 0.018

8.488 ± 0.039

4.835 ± 0.024

6.247 ± 0.025

8.499 ± 0.029

1.502 ± 0.012

2.084 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski