Streptococcus satellite phage Javan416

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZML9|A0A4D5ZML9_9VIRU DNA-damage-inducible protein D OS=Streptococcus satellite phage Javan416 OX=2558691 GN=JavanS416_0003 PE=4 SV=1
MM1 pKa = 7.6TNTNKK6 pKa = 9.01STAPMTLAEE15 pKa = 4.04LKK17 pKa = 10.37AWVEE21 pKa = 4.41EE22 pKa = 4.25TCEE25 pKa = 4.49LSEE28 pKa = 4.68LLQTKK33 pKa = 9.63DD34 pKa = 3.47YY35 pKa = 11.13KK36 pKa = 11.15SYY38 pKa = 10.88LPKK41 pKa = 10.32EE42 pKa = 4.08KK43 pKa = 10.35KK44 pKa = 10.29EE45 pKa = 3.9EE46 pKa = 3.95FEE48 pKa = 5.12AISFSVFNCLEE59 pKa = 4.21SFSMMLEE66 pKa = 4.18DD67 pKa = 6.05NEE69 pKa = 4.97LHH71 pKa = 5.74YY72 pKa = 10.58QPKK75 pKa = 9.84PIEE78 pKa = 4.32EE79 pKa = 4.24EE80 pKa = 3.95SDD82 pKa = 3.39LDD84 pKa = 3.84NQADD88 pKa = 3.67EE89 pKa = 4.13MAQYY93 pKa = 10.71QEE95 pKa = 4.03LAKK98 pKa = 10.21EE99 pKa = 4.34VEE101 pKa = 4.39GEE103 pKa = 4.07EE104 pKa = 3.83QGARR108 pKa = 11.84GLAYY112 pKa = 10.24DD113 pKa = 4.22DD114 pKa = 5.18TIHH117 pKa = 7.15EE118 pKa = 4.79YY119 pKa = 9.73IDD121 pKa = 3.8KK122 pKa = 9.55RR123 pKa = 11.84TEE125 pKa = 3.8QLKK128 pKa = 9.5EE129 pKa = 3.5QATFEE134 pKa = 4.06EE135 pKa = 5.51LISKK139 pKa = 8.92VALYY143 pKa = 10.35EE144 pKa = 4.59SEE146 pKa = 4.11LLDD149 pKa = 3.57YY150 pKa = 11.28AEE152 pKa = 5.23RR153 pKa = 11.84LLSDD157 pKa = 3.74EE158 pKa = 4.79PLNADD163 pKa = 3.49SEE165 pKa = 4.84TAIGTLDD172 pKa = 3.63MLDD175 pKa = 4.84DD176 pKa = 3.92EE177 pKa = 6.32AIDD180 pKa = 3.89LFKK183 pKa = 11.26SVDD186 pKa = 3.58VNDD189 pKa = 4.21EE190 pKa = 4.28YY191 pKa = 11.53QGLEE195 pKa = 4.1YY196 pKa = 11.25YY197 pKa = 8.2NTSLNKK203 pKa = 10.18

Molecular weight: 23.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZNB0|A0A4D5ZNB0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan416 OX=2558691 GN=JavanS416_0011 PE=4 SV=1
MM1 pKa = 7.51NIKK4 pKa = 10.04EE5 pKa = 4.27VIKK8 pKa = 11.1KK9 pKa = 10.3NGTKK13 pKa = 9.93VYY15 pKa = 10.29RR16 pKa = 11.84SNVYY20 pKa = 10.53LGVDD24 pKa = 4.04SITGKK29 pKa = 10.2KK30 pKa = 10.02VKK32 pKa = 8.29TTITGRR38 pKa = 11.84TKK40 pKa = 10.86KK41 pKa = 9.59EE42 pKa = 3.86VKK44 pKa = 10.34AKK46 pKa = 10.27AQQAQSDD53 pKa = 4.36FKK55 pKa = 11.76ANGATVFKK63 pKa = 9.87ATNIRR68 pKa = 11.84NYY70 pKa = 10.74KK71 pKa = 9.59EE72 pKa = 5.24LADD75 pKa = 4.06LWLEE79 pKa = 4.09NYY81 pKa = 10.24QMTIKK86 pKa = 9.9PQTVDD91 pKa = 3.23VVKK94 pKa = 10.59SYY96 pKa = 9.64LTIHH100 pKa = 7.02ILPSFGAMPLEE111 pKa = 4.7NISIVVIQDD120 pKa = 3.28WVNKK124 pKa = 9.18LAKK127 pKa = 10.29KK128 pKa = 9.95LVNFSAVVSVHH139 pKa = 6.55KK140 pKa = 10.61RR141 pKa = 11.84ILQYY145 pKa = 11.21AVTMQLIDD153 pKa = 3.77YY154 pKa = 9.29NPARR158 pKa = 11.84EE159 pKa = 4.23VILPRR164 pKa = 11.84KK165 pKa = 8.96PKK167 pKa = 10.64AKK169 pKa = 9.66TKK171 pKa = 10.54VKK173 pKa = 10.54YY174 pKa = 10.3IPNDD178 pKa = 3.17NLKK181 pKa = 10.36IFMDD185 pKa = 4.16YY186 pKa = 9.59MDD188 pKa = 5.92KK189 pKa = 10.57IAPYY193 pKa = 9.82QYY195 pKa = 10.83KK196 pKa = 10.46DD197 pKa = 3.44YY198 pKa = 10.81YY199 pKa = 11.57DD200 pKa = 4.56NILYY204 pKa = 10.16HH205 pKa = 7.0LLLATGCRR213 pKa = 11.84FGEE216 pKa = 4.58VVALEE221 pKa = 4.21WSDD224 pKa = 3.89IDD226 pKa = 4.37LKK228 pKa = 11.39NGTVSINKK236 pKa = 9.6NYY238 pKa = 10.59SHH240 pKa = 7.47ISNAVSTVKK249 pKa = 10.63SKK251 pKa = 11.09AGNRR255 pKa = 11.84IISIDD260 pKa = 3.44NKK262 pKa = 9.44TILMLKK268 pKa = 9.54QYY270 pKa = 10.69RR271 pKa = 11.84NRR273 pKa = 11.84QRR275 pKa = 11.84QIFVEE280 pKa = 4.43VGTTAPSVVFATPTQTYY297 pKa = 7.11MQIGNRR303 pKa = 11.84QHH305 pKa = 6.86ALKK308 pKa = 8.95THH310 pKa = 6.91LKK312 pKa = 9.32ACNIPRR318 pKa = 11.84FTFHH322 pKa = 7.7AFRR325 pKa = 11.84HH326 pKa = 4.69THH328 pKa = 6.92ASLLLNAGISYY339 pKa = 10.65KK340 pKa = 10.3EE341 pKa = 3.81LQHH344 pKa = 7.05RR345 pKa = 11.84LGHH348 pKa = 6.42ANITMTLDD356 pKa = 3.31IYY358 pKa = 11.44SHH360 pKa = 6.78LSKK363 pKa = 10.68EE364 pKa = 4.26KK365 pKa = 9.36EE366 pKa = 4.04KK367 pKa = 11.02EE368 pKa = 3.79AVFYY372 pKa = 10.34YY373 pKa = 10.61EE374 pKa = 5.59KK375 pKa = 10.93ALQNLL380 pKa = 3.78

Molecular weight: 43.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17

0

17

3256

52

536

191.5

22.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.651 ± 0.429

0.43 ± 0.074

5.897 ± 0.454

9.029 ± 1.001

4.208 ± 0.405

4.33 ± 0.363

1.812 ± 0.286

7.924 ± 0.606

9.306 ± 0.435

9.92 ± 0.743

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.119 ± 0.264

7.371 ± 0.516

2.733 ± 0.562

4.023 ± 0.302

4.668 ± 0.396

4.853 ± 0.346

5.283 ± 0.377

5.068 ± 0.516

0.768 ± 0.087

4.607 ± 0.349
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski