Thalassomonas sp. M1454

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Colwelliaceae; Thalassomonas; unclassified Thalassomonas

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3137 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A553FLB4|A0A553FLB4_9GAMM DUF416 family protein OS=Thalassomonas sp. M1454 OX=2594477 GN=FNN08_01170 PE=4 SV=1
MM1 pKa = 7.72NSLLKK6 pKa = 9.84KK7 pKa = 7.03TTILAALSVSLLGCDD22 pKa = 5.09DD23 pKa = 3.56VDD25 pKa = 3.78EE26 pKa = 5.87GYY28 pKa = 11.68ANNFKK33 pKa = 10.79NDD35 pKa = 3.43NPVGFSMEE43 pKa = 4.06NIAAEE48 pKa = 4.13FEE50 pKa = 4.47EE51 pKa = 4.72VSGIQQVNLLEE62 pKa = 4.34GAMVDD67 pKa = 4.26GEE69 pKa = 4.47PLTLEE74 pKa = 4.45SSSSPIYY81 pKa = 10.3IRR83 pKa = 11.84DD84 pKa = 3.73FNFEE88 pKa = 4.15TLTDD92 pKa = 3.93GFTTPQAPGFANNQGVSPFTIGDD115 pKa = 3.63DD116 pKa = 3.84GVTLEE121 pKa = 4.52IDD123 pKa = 2.99TDD125 pKa = 3.8AFAEE129 pKa = 4.18ALRR132 pKa = 11.84MCDD135 pKa = 3.27PTDD138 pKa = 3.11VRR140 pKa = 11.84GAVDD144 pKa = 3.79EE145 pKa = 4.32NNNPIGNGVRR155 pKa = 11.84DD156 pKa = 4.29FPTLVEE162 pKa = 3.82YY163 pKa = 10.87SITYY167 pKa = 9.89VVDD170 pKa = 3.03NGYY173 pKa = 10.68EE174 pKa = 3.88YY175 pKa = 11.26APGEE179 pKa = 4.07EE180 pKa = 4.34HH181 pKa = 7.3PVRR184 pKa = 11.84TLEE187 pKa = 3.97LTITAGSDD195 pKa = 3.12PVANVQAFDD204 pKa = 3.76INVASGDD211 pKa = 4.27SVPMLSATAPAYY223 pKa = 10.59SCNSNLTYY231 pKa = 10.83DD232 pKa = 3.68VADD235 pKa = 3.69TSIASVDD242 pKa = 3.61EE243 pKa = 4.45NGVITGNNKK252 pKa = 9.36GQTTITVASVEE263 pKa = 4.24NPEE266 pKa = 4.1LTATATINVTPGFNLLITNQEE287 pKa = 4.18FNDD290 pKa = 3.6LGAPLGTKK298 pKa = 9.83AVPTCSAIGVNVEE311 pKa = 3.78PTIVADD317 pKa = 4.13EE318 pKa = 4.31LSGEE322 pKa = 4.12YY323 pKa = 9.78TYY325 pKa = 11.59AWSSDD330 pKa = 3.4SLVLAKK336 pKa = 10.72EE337 pKa = 4.0EE338 pKa = 4.32TTGGFSATGRR348 pKa = 11.84FANTLAVGEE357 pKa = 4.53SADD360 pKa = 3.28ITVGYY365 pKa = 10.26GSGYY369 pKa = 10.11SGSTAAGDD377 pKa = 3.78VQPQMVTVTAEE388 pKa = 3.95RR389 pKa = 11.84NLACDD394 pKa = 3.93PGLNADD400 pKa = 3.77GTDD403 pKa = 4.39FFASDD408 pKa = 4.28LKK410 pKa = 11.04LDD412 pKa = 3.83SGNWGASATHH422 pKa = 5.58VAEE425 pKa = 4.55GLDD428 pKa = 3.75GTSLQITSTNGGKK441 pKa = 10.31FYY443 pKa = 11.16GGVVQQQWNQVFNYY457 pKa = 9.66HH458 pKa = 6.49AATYY462 pKa = 9.87GRR464 pKa = 11.84GGASIGRR471 pKa = 11.84TFKK474 pKa = 10.86FKK476 pKa = 10.1VWAKK480 pKa = 10.35LGQLPTEE487 pKa = 4.23EE488 pKa = 4.44VKK490 pKa = 10.98LEE492 pKa = 3.89HH493 pKa = 7.39SIIPWNCDD501 pKa = 2.68GCDD504 pKa = 3.49GLANYY509 pKa = 7.89PARR512 pKa = 11.84IEE514 pKa = 4.18AGVAGTVTAVLKK526 pKa = 8.41PTTDD530 pKa = 2.87WQLVEE535 pKa = 5.78FINPLTGTDD544 pKa = 3.29EE545 pKa = 4.33WSVPAHH551 pKa = 5.88FNVSTAAFLSWDD563 pKa = 3.08IYY565 pKa = 11.26GFADD569 pKa = 4.59GEE571 pKa = 4.63TILLDD576 pKa = 3.4NYY578 pKa = 10.96SIVEE582 pKa = 4.05VEE584 pKa = 3.87

Molecular weight: 61.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A553FLL8|A0A553FLL8_9GAMM Probable chromosome-partitioning protein ParB OS=Thalassomonas sp. M1454 OX=2594477 GN=FNN08_01690 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.16RR12 pKa = 11.84KK13 pKa = 9.08RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.33NGRR28 pKa = 11.84AVIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.45GRR39 pKa = 11.84AKK41 pKa = 9.64LTAA44 pKa = 4.21

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3137

0

3137

1074329

37

3053

342.5

38.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.278 ± 0.044

0.992 ± 0.015

5.805 ± 0.041

6.155 ± 0.037

4.419 ± 0.031

6.556 ± 0.041

2.045 ± 0.019

7.028 ± 0.034

6.067 ± 0.045

9.933 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.402 ± 0.022

5.17 ± 0.038

3.672 ± 0.025

4.399 ± 0.035

3.7 ± 0.03

6.781 ± 0.035

5.453 ± 0.03

6.778 ± 0.037

1.152 ± 0.015

3.216 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski