Pedobacter sp. BS3

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Pedobacter; unclassified Pedobacter

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3935 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5D8YRS3|A0A5D8YRS3_9SPHI Sugar phosphate isomerase/epimerase OS=Pedobacter sp. BS3 OX=2567937 GN=FW774_07940 PE=4 SV=1
MM1 pKa = 7.25IQQINTSEE9 pKa = 3.9ASVYY13 pKa = 10.13VGTYY17 pKa = 9.71GKK19 pKa = 10.68YY20 pKa = 9.48NDD22 pKa = 3.85GSIFGEE28 pKa = 3.98WLKK31 pKa = 11.3LSDD34 pKa = 3.87YY35 pKa = 11.18ADD37 pKa = 3.26KK38 pKa = 11.44KK39 pKa = 10.83EE40 pKa = 4.41FYY42 pKa = 8.34TACKK46 pKa = 9.65EE47 pKa = 4.07LHH49 pKa = 6.45NDD51 pKa = 3.23EE52 pKa = 5.29ADD54 pKa = 3.6PEE56 pKa = 4.99FMFQDD61 pKa = 4.05YY62 pKa = 11.31EE63 pKa = 4.3NIPNGLIGEE72 pKa = 4.58CWLSDD77 pKa = 3.74NIFDD81 pKa = 4.45VLEE84 pKa = 4.29ALEE87 pKa = 5.54DD88 pKa = 3.65MDD90 pKa = 5.27EE91 pKa = 4.2SRR93 pKa = 11.84QEE95 pKa = 5.65AFLLWCDD102 pKa = 3.71NKK104 pKa = 10.45SHH106 pKa = 7.0KK107 pKa = 10.54LSEE110 pKa = 4.65DD111 pKa = 4.35DD112 pKa = 4.64INDD115 pKa = 5.03LISDD119 pKa = 4.52FDD121 pKa = 4.7DD122 pKa = 5.79DD123 pKa = 5.69YY124 pKa = 11.54IGEE127 pKa = 4.34YY128 pKa = 10.1KK129 pKa = 10.71DD130 pKa = 4.44EE131 pKa = 4.16EE132 pKa = 4.36DD133 pKa = 3.61FAYY136 pKa = 10.11EE137 pKa = 4.45QIEE140 pKa = 4.33QMDD143 pKa = 4.03LPEE146 pKa = 3.89FAKK149 pKa = 10.44RR150 pKa = 11.84YY151 pKa = 9.12FDD153 pKa = 3.85YY154 pKa = 11.11EE155 pKa = 3.78AFARR159 pKa = 11.84DD160 pKa = 4.99LFCCDD165 pKa = 2.87YY166 pKa = 10.6WSDD169 pKa = 3.77NGHH172 pKa = 5.71VFYY175 pKa = 11.3NSS177 pKa = 2.81

Molecular weight: 20.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5D8YRU3|A0A5D8YRU3_9SPHI Arylsulfatase OS=Pedobacter sp. BS3 OX=2567937 GN=FW774_00040 PE=3 SV=1
MM1 pKa = 7.54GKK3 pKa = 9.96AEE5 pKa = 4.56KK6 pKa = 10.66NGFSPFRR13 pKa = 11.84PQLPYY18 pKa = 11.33ANDD21 pKa = 3.56FSIYY25 pKa = 9.4PLHH28 pKa = 6.64LSPYY32 pKa = 7.94KK33 pKa = 10.84VRR35 pKa = 11.84VAFPNDD41 pKa = 3.3CPVAHH46 pKa = 6.43NQTLPTNAPNYY57 pKa = 8.88RR58 pKa = 11.84IKK60 pKa = 10.56RR61 pKa = 11.84VHH63 pKa = 6.7RR64 pKa = 11.84NNLLLCAISHH74 pKa = 6.88ASLFAGIRR82 pKa = 11.84SRR84 pKa = 11.84WQLGKK89 pKa = 9.59PEE91 pKa = 3.93RR92 pKa = 11.84RR93 pKa = 11.84IAGTPEE99 pKa = 3.76RR100 pKa = 11.84MTAFHH105 pKa = 6.28TAGRR109 pKa = 11.84NNGQHH114 pKa = 5.99EE115 pKa = 5.21SYY117 pKa = 10.39PVAIPSGRR125 pKa = 11.84TIGKK129 pKa = 9.39PSVLPAA135 pKa = 4.35

Molecular weight: 15.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3935

0

3935

1391832

31

5456

353.7

39.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.581 ± 0.035

0.817 ± 0.013

5.398 ± 0.029

5.475 ± 0.042

4.648 ± 0.028

6.911 ± 0.039

1.888 ± 0.018

7.128 ± 0.035

6.739 ± 0.037

9.277 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.109 ± 0.019

5.714 ± 0.038

3.945 ± 0.027

3.844 ± 0.025

4.211 ± 0.025

6.31 ± 0.032

6.029 ± 0.047

6.334 ± 0.027

1.256 ± 0.018

4.388 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski