Escherichia phage Fraca

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A653FWP6|A0A653FWP6_9CAUD Phage protein OS=Escherichia phage Fraca OX=2562643 PE=4 SV=1
MM1 pKa = 7.01ITLSGKK7 pKa = 10.17DD8 pKa = 3.6LMEE11 pKa = 4.69LLDD14 pKa = 4.5YY15 pKa = 10.82ISPDD19 pKa = 3.34RR20 pKa = 11.84EE21 pKa = 4.0SDD23 pKa = 3.68PEE25 pKa = 3.92QLEE28 pKa = 4.64TEE30 pKa = 4.31VSVIKK35 pKa = 9.83MDD37 pKa = 3.39KK38 pKa = 10.13TFASSEE44 pKa = 3.99GDD46 pKa = 3.01IRR48 pKa = 11.84PAGVYY53 pKa = 10.03AYY55 pKa = 8.85LTEE58 pKa = 4.22YY59 pKa = 10.28PEE61 pKa = 4.29EE62 pKa = 4.23GLYY65 pKa = 10.96GPIGSVEE72 pKa = 3.81

Molecular weight: 7.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A653FWL9|A0A653FWL9_9CAUD Uncharacterized protein OS=Escherichia phage Fraca OX=2562643 PE=4 SV=1
MM1 pKa = 7.36NDD3 pKa = 2.91KK4 pKa = 10.84RR5 pKa = 11.84ITTTSIDD12 pKa = 3.35RR13 pKa = 11.84AFTTEE18 pKa = 4.06LQPVYY23 pKa = 10.31VVSRR27 pKa = 11.84HH28 pKa = 4.9GYY30 pKa = 7.33SRR32 pKa = 11.84RR33 pKa = 11.84FLSRR37 pKa = 11.84SAAISNLAHH46 pKa = 6.14YY47 pKa = 7.81MVTKK51 pKa = 8.28TFRR54 pKa = 11.84RR55 pKa = 11.84AGLNTNEE62 pKa = 4.02PDD64 pKa = 3.32EE65 pKa = 4.6PVFSNGVLVNRR76 pKa = 11.84MGQHH80 pKa = 4.42TQQYY84 pKa = 9.07LFAHH88 pKa = 5.91NRR90 pKa = 11.84CMRR93 pKa = 11.84RR94 pKa = 11.84IRR96 pKa = 11.84RR97 pKa = 11.84ILEE100 pKa = 3.89RR101 pKa = 11.84KK102 pKa = 9.24RR103 pKa = 11.84EE104 pKa = 4.05ARR106 pKa = 11.84KK107 pKa = 8.62WLAKK111 pKa = 8.53WDD113 pKa = 4.02SMHH116 pKa = 7.1DD117 pKa = 3.42RR118 pKa = 11.84YY119 pKa = 11.17VKK121 pKa = 10.31EE122 pKa = 3.92QAEE125 pKa = 4.31LQASKK130 pKa = 11.08PEE132 pKa = 4.73GINN135 pKa = 3.16

Molecular weight: 15.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

13158

37

1148

202.4

22.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.971 ± 0.584

1.148 ± 0.176

5.624 ± 0.244

6.422 ± 0.363

3.321 ± 0.237

7.668 ± 0.346

1.809 ± 0.232

5.601 ± 0.234

5.738 ± 0.3

7.668 ± 0.276

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.728 ± 0.174

4.4 ± 0.228

3.701 ± 0.246

4.309 ± 0.327

5.662 ± 0.299

6.627 ± 0.422

6.088 ± 0.329

6.802 ± 0.26

1.756 ± 0.137

2.956 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski