Acinetobacter virus 133
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 257 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D9I693|D9I693_9CAUD Gp8 baseplate wedge subunit OS=Acinetobacter virus 133 OX=279006 GN=8 PE=4 SV=1
MM1 pKa = 7.34 TLDD4 pKa = 3.49 PQGPLLEE11 pKa = 4.55 PVLEE15 pKa = 4.56 GEE17 pKa = 4.16 QFDD20 pKa = 3.88 IEE22 pKa = 4.71 FIGDD26 pKa = 3.52 YY27 pKa = 7.4 TTIDD31 pKa = 2.99 IVLLDD36 pKa = 4.36 FPDD39 pKa = 3.92 TVTVTGNKK47 pKa = 8.28 IHH49 pKa = 6.74 GVFNLVFDD57 pKa = 5.52 LPNQGLKK64 pKa = 10.47 YY65 pKa = 9.94 RR66 pKa = 11.84 DD67 pKa = 3.31 GMDD70 pKa = 3.34 YY71 pKa = 11.39 GSTSQFKK78 pKa = 10.24 DD79 pKa = 3.44 VPEE82 pKa = 4.63 DD83 pKa = 3.51 ADD85 pKa = 3.52 IYY87 pKa = 10.97 SYY89 pKa = 11.15 SPPVGSIRR97 pKa = 11.84 NYY99 pKa = 9.12 NLRR102 pKa = 11.84 VNITYY107 pKa = 10.17 TDD109 pKa = 3.24 IATGGGEE116 pKa = 3.76 QSEE119 pKa = 4.79 TIEE122 pKa = 4.39 YY123 pKa = 9.99 ILPVDD128 pKa = 3.85 INYY131 pKa = 10.56 SLYY134 pKa = 10.96 AQALRR139 pKa = 11.84 DD140 pKa = 4.03 ALWQQQ145 pKa = 3.17
Molecular weight: 16.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.783
IPC_protein 3.77
Toseland 3.554
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.719
Rodwell 3.592
Grimsley 3.465
Solomon 3.757
Lehninger 3.706
Nozaki 3.884
DTASelect 4.139
Thurlkill 3.617
EMBOSS 3.732
Sillero 3.897
Patrickios 0.655
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.776
Protein with the highest isoelectric point:
>tr|D9I5Z4|D9I5Z4_9CAUD Uncharacterized protein OS=Acinetobacter virus 133 OX=279006 GN=Acj133p028 PE=4 SV=1
MM1 pKa = 7.35 IKK3 pKa = 10.16 EE4 pKa = 4.36 SPLMLSIRR12 pKa = 11.84 TKK14 pKa = 11.01 LDD16 pKa = 2.96 WSINAVNSAQVEE28 pKa = 4.18 INRR31 pKa = 11.84 AGKK34 pKa = 9.13 VKK36 pKa = 10.34 RR37 pKa = 11.84 RR38 pKa = 11.84 LQHH41 pKa = 5.94 RR42 pKa = 11.84 QLSYY46 pKa = 9.36 GNSRR50 pKa = 11.84 VRR52 pKa = 11.84 EE53 pKa = 3.93 QAIKK57 pKa = 10.74 EE58 pKa = 3.73 MDD60 pKa = 3.16 RR61 pKa = 11.84 RR62 pKa = 11.84 ILKK65 pKa = 9.4 FARR68 pKa = 11.84 LQQKK72 pKa = 10.24 SLLDD76 pKa = 3.91 RR77 pKa = 11.84 NALSALYY84 pKa = 10.01 VQLRR88 pKa = 11.84 EE89 pKa = 4.23 VKK91 pKa = 10.07 NGHH94 pKa = 5.68 SKK96 pKa = 10.44 RR97 pKa = 11.84 DD98 pKa = 3.29 QAYY101 pKa = 9.38 YY102 pKa = 9.81 LHH104 pKa = 7.2 QYY106 pKa = 7.16 MLKK109 pKa = 10.48 SGVLL113 pKa = 3.48
Molecular weight: 13.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.31
IPC2_protein 9.955
IPC_protein 10.76
Toseland 10.891
ProMoST 10.584
Dawson 10.979
Bjellqvist 10.687
Wikipedia 11.184
Rodwell 11.257
Grimsley 11.023
Solomon 11.096
Lehninger 11.067
Nozaki 10.847
DTASelect 10.687
Thurlkill 10.877
EMBOSS 11.286
Sillero 10.906
Patrickios 10.979
IPC_peptide 11.111
IPC2_peptide 9.472
IPC2.peptide.svr19 8.4
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
257
0
257
50748
35
1277
197.5
22.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.678 ± 0.212
1.188 ± 0.075
6.138 ± 0.118
6.215 ± 0.167
4.111 ± 0.111
6.111 ± 0.211
2.191 ± 0.113
6.95 ± 0.124
6.897 ± 0.193
8.247 ± 0.163
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.905 ± 0.101
5.547 ± 0.108
3.825 ± 0.093
3.943 ± 0.109
4.465 ± 0.099
6.142 ± 0.137
6.25 ± 0.24
6.779 ± 0.116
1.328 ± 0.059
4.091 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here