Thiovulum sp. ES

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Thiovulaceae; Thiovulum; unclassified Thiovulum

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2104 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J0L8H2|J0L8H2_9PROT 3-oxoacyl-[acyl-carrier-protein] synthase 2 OS=Thiovulum sp. ES OX=1177931 GN=ThvES_00001340 PE=3 SV=1
MM1 pKa = 7.52KK2 pKa = 10.44NISLVVATLLVSSSTLFAEE21 pKa = 4.93DD22 pKa = 4.82NKK24 pKa = 10.98NDD26 pKa = 3.51WEE28 pKa = 4.31FSGNLRR34 pKa = 11.84TGYY37 pKa = 8.08TAEE40 pKa = 4.45TVDD43 pKa = 4.7APNTDD48 pKa = 3.07SVTNTGAATGGWLTLQTPKK67 pKa = 10.74VNTFSVTASLFTSQILMGQKK87 pKa = 10.07SDD89 pKa = 3.01WWLHH93 pKa = 5.27SGGAGEE99 pKa = 4.77TNGKK103 pKa = 9.89SYY105 pKa = 10.35TYY107 pKa = 10.32FGEE110 pKa = 4.48AYY112 pKa = 9.64ISGEE116 pKa = 4.18LFSSDD121 pKa = 4.09SIIGKK126 pKa = 8.01TDD128 pKa = 2.77ITIGRR133 pKa = 11.84KK134 pKa = 9.61VIEE137 pKa = 4.35TPFANSDD144 pKa = 3.85DD145 pKa = 4.15MGMSPDD151 pKa = 3.63SFEE154 pKa = 5.58LAMLQANPIEE164 pKa = 4.35NLTVIAARR172 pKa = 11.84ATKK175 pKa = 10.13IGGYY179 pKa = 9.16DD180 pKa = 3.32APLAGAKK187 pKa = 10.28SKK189 pKa = 11.56DD190 pKa = 3.45EE191 pKa = 4.19FVEE194 pKa = 4.19LTQGDD199 pKa = 4.42GVSIVAGLYY208 pKa = 9.72SDD210 pKa = 4.44EE211 pKa = 4.28EE212 pKa = 4.81AGISAQAWNYY222 pKa = 10.46YY223 pKa = 10.46LDD225 pKa = 4.83DD226 pKa = 3.8VDD228 pKa = 4.99GAGLNMSLTYY238 pKa = 10.95ADD240 pKa = 4.56FVYY243 pKa = 10.42TMSLSEE249 pKa = 4.42DD250 pKa = 3.27MSVSVGAEE258 pKa = 3.41YY259 pKa = 11.26GLFQEE264 pKa = 5.61LNFDD268 pKa = 4.02EE269 pKa = 5.64KK270 pKa = 11.16DD271 pKa = 3.37GSLVGGKK278 pKa = 9.12IEE280 pKa = 5.06ASISDD285 pKa = 4.19LSLGVAMDD293 pKa = 4.01TASGDD298 pKa = 3.37IAPQGGFSFAPYY310 pKa = 8.41YY311 pKa = 10.31TIANIYY317 pKa = 8.31LTIAAPGVTDD327 pKa = 3.56ITAFSGKK334 pKa = 9.95ADD336 pKa = 3.68YY337 pKa = 10.66QVTEE341 pKa = 4.2EE342 pKa = 4.64LSLSALYY349 pKa = 8.04MTMSSDD355 pKa = 4.49NLDD358 pKa = 4.43DD359 pKa = 4.66DD360 pKa = 3.72WSEE363 pKa = 4.12VDD365 pKa = 4.76LSASYY370 pKa = 10.77SVSDD374 pKa = 5.26DD375 pKa = 3.81LALDD379 pKa = 4.46LYY381 pKa = 10.09MATWNEE387 pKa = 3.95GEE389 pKa = 4.29KK390 pKa = 11.0DD391 pKa = 3.46FTRR394 pKa = 11.84YY395 pKa = 8.43TIFANYY401 pKa = 10.17SFF403 pKa = 4.57

Molecular weight: 43.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J0UXR4|J0UXR4_9PROT Histidine kinase OS=Thiovulum sp. ES OX=1177931 GN=ThvES_00013220 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.04QPHH8 pKa = 4.86NTPRR12 pKa = 11.84KK13 pKa = 7.34RR14 pKa = 11.84THH16 pKa = 6.22GFRR19 pKa = 11.84TRR21 pKa = 11.84MKK23 pKa = 8.5TKK25 pKa = 9.48NGRR28 pKa = 11.84RR29 pKa = 11.84VISARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.87GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2104

0

2104

632618

22

4349

300.7

34.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.005 ± 0.071

0.543 ± 0.016

5.792 ± 0.05

8.525 ± 0.063

5.7 ± 0.056

6.097 ± 0.071

1.557 ± 0.025

8.504 ± 0.055

8.701 ± 0.084

9.235 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.003 ± 0.027

5.813 ± 0.049

2.78 ± 0.03

2.772 ± 0.026

3.815 ± 0.042

7.634 ± 0.064

5.166 ± 0.071

6.059 ± 0.04

0.794 ± 0.018

3.504 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski