Lactobacillus perolens DSM 12744
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3052 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R1MUT1|A0A0R1MUT1_9LACO Uncharacterized protein OS=Lactobacillus perolens DSM 12744 OX=1423792 GN=FD09_GL000552 PE=4 SV=1
MM1 pKa = 7.65 NDD3 pKa = 2.77 QVTLVNLDD11 pKa = 3.83 DD12 pKa = 4.3 FQLHH16 pKa = 5.54 YY17 pKa = 11.04 EE18 pKa = 4.78 RR19 pKa = 11.84 IADD22 pKa = 4.01 FDD24 pKa = 4.08 NGASPTGDD32 pKa = 3.47 VIINLIDD39 pKa = 3.89 AANHH43 pKa = 6.92 AIQAGMNACDD53 pKa = 5.33 LILASEE59 pKa = 4.26 QCAKK63 pKa = 10.63 PEE65 pKa = 4.14 AYY67 pKa = 10.45 LKK69 pKa = 10.56 GARR72 pKa = 11.84 FSFNVSSSPLGLVIGYY88 pKa = 9.95 DD89 pKa = 3.49 GVNIDD94 pKa = 3.79 EE95 pKa = 4.42
Molecular weight: 10.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.761
IPC2_protein 3.948
IPC_protein 3.897
Toseland 3.681
ProMoST 4.024
Dawson 3.897
Bjellqvist 4.139
Wikipedia 3.872
Rodwell 3.719
Grimsley 3.592
Solomon 3.884
Lehninger 3.846
Nozaki 4.024
DTASelect 4.279
Thurlkill 3.757
EMBOSS 3.872
Sillero 4.024
Patrickios 1.939
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.942
Protein with the highest isoelectric point:
>tr|A0A0R1MXB5|A0A0R1MXB5_9LACO HMA domain-containing protein OS=Lactobacillus perolens DSM 12744 OX=1423792 GN=FD09_GL002417 PE=4 SV=1
MM1 pKa = 6.51 STKK4 pKa = 9.41 RR5 pKa = 11.84 TYY7 pKa = 9.75 QPKK10 pKa = 9.21 KK11 pKa = 7.35 IHH13 pKa = 6.61 RR14 pKa = 11.84 ARR16 pKa = 11.84 VHH18 pKa = 6.16 GFMKK22 pKa = 10.5 RR23 pKa = 11.84 MSSKK27 pKa = 10.59 NGRR30 pKa = 11.84 KK31 pKa = 8.97 VLARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 AKK39 pKa = 9.94 GRR41 pKa = 11.84 KK42 pKa = 8.67 VLSAA46 pKa = 4.05
Molecular weight: 5.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.391
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.384
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.106
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 8.993
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3052
0
3052
963946
44
3066
315.8
34.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.486 ± 0.045
0.375 ± 0.008
5.9 ± 0.046
4.484 ± 0.041
4.131 ± 0.037
7.012 ± 0.038
2.379 ± 0.023
6.46 ± 0.043
5.016 ± 0.041
9.656 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.629 ± 0.022
4.31 ± 0.032
4.275 ± 0.029
5.044 ± 0.042
4.368 ± 0.038
5.5 ± 0.034
6.908 ± 0.055
7.266 ± 0.032
1.315 ± 0.021
3.487 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here