Adelaide River virus (ARV)
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K4HH67|K4HH67_ARV Beta protein OS=Adelaide River virus OX=31612 GN=beta PE=4 SV=1
MM1 pKa = 7.6 EE2 pKa = 5.49 KK3 pKa = 10.51 FNPSKK8 pKa = 11.13 VLGEE12 pKa = 4.11 YY13 pKa = 9.21 DD14 pKa = 3.57 TDD16 pKa = 4.18 KK17 pKa = 11.38 LIASINEE24 pKa = 4.43 TNWNLDD30 pKa = 3.41 DD31 pKa = 5.58 DD32 pKa = 5.12 PEE34 pKa = 4.47 FSAPQEE40 pKa = 4.31 SIEE43 pKa = 4.19 PSNSIDD49 pKa = 3.37 NYY51 pKa = 9.63 RR52 pKa = 11.84 DD53 pKa = 3.5 EE54 pKa = 4.06 IVKK57 pKa = 10.36 IGVCLSSNNQDD68 pKa = 3.35 YY69 pKa = 11.6 SFLDD73 pKa = 3.43 NQKK76 pKa = 9.89 EE77 pKa = 4.06 KK78 pKa = 10.96 EE79 pKa = 4.04 IFLEE83 pKa = 4.67 EE84 pKa = 4.0 EE85 pKa = 3.92 NDD87 pKa = 3.54 WEE89 pKa = 4.85 VEE91 pKa = 4.29 FTKK94 pKa = 10.74 KK95 pKa = 10.84 LEE97 pKa = 4.07 DD98 pKa = 3.46 HH99 pKa = 6.44 NVPNEE104 pKa = 4.13 SEE106 pKa = 4.89 LEE108 pKa = 4.06 VQINIEE114 pKa = 4.06 DD115 pKa = 5.14 LIALLHH121 pKa = 5.76 FLNVEE126 pKa = 3.71 IDD128 pKa = 3.43 QYY130 pKa = 11.91 NILPLSLQNKK140 pKa = 9.52 VILQRR145 pKa = 11.84 KK146 pKa = 9.12 KK147 pKa = 10.45 EE148 pKa = 4.06 GKK150 pKa = 9.84 SDD152 pKa = 3.49 DD153 pKa = 4.06 KK154 pKa = 11.25 KK155 pKa = 10.39 RR156 pKa = 11.84 QNGKK160 pKa = 9.83 GDD162 pKa = 3.66 KK163 pKa = 10.24 KK164 pKa = 10.94 SCPDD168 pKa = 4.58 SKK170 pKa = 11.17 NQSNNCQTMNAEE182 pKa = 4.69 TNGSTNLGADD192 pKa = 3.65 RR193 pKa = 11.84 GPNQTEE199 pKa = 4.57 DD200 pKa = 3.44 NSSMLEE206 pKa = 3.96 NDD208 pKa = 3.4 SEE210 pKa = 5.74 AILDD214 pKa = 3.76 SDD216 pKa = 4.4 GGEE219 pKa = 3.79 LFQFIIKK226 pKa = 9.65 TMDD229 pKa = 2.66 QGIRR233 pKa = 11.84 VKK235 pKa = 10.8 KK236 pKa = 9.93 KK237 pKa = 9.66 FSGKK241 pKa = 8.99 KK242 pKa = 9.31 VKK244 pKa = 8.69 ITRR247 pKa = 11.84 EE248 pKa = 4.08 NIGLDD253 pKa = 3.08 YY254 pKa = 11.02 HH255 pKa = 6.95 VINGHH260 pKa = 4.91 IHH262 pKa = 6.03 GKK264 pKa = 7.33 SAKK267 pKa = 10.29 DD268 pKa = 3.52 SIDD271 pKa = 3.28 LKK273 pKa = 11.19 KK274 pKa = 10.92 LMRR277 pKa = 11.84 EE278 pKa = 4.37 LIKK281 pKa = 10.61 KK282 pKa = 7.77 GRR284 pKa = 11.84 KK285 pKa = 8.52 YY286 pKa = 10.86 KK287 pKa = 10.57 DD288 pKa = 3.21 YY289 pKa = 11.42 SNQLDD294 pKa = 3.51 LSEE297 pKa = 5.02 IEE299 pKa = 4.29 FF300 pKa = 4.22
Molecular weight: 34.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.879
IPC2_protein 4.813
IPC_protein 4.762
Toseland 4.622
ProMoST 4.863
Dawson 4.711
Bjellqvist 4.851
Wikipedia 4.571
Rodwell 4.609
Grimsley 4.533
Solomon 4.711
Lehninger 4.66
Nozaki 4.813
DTASelect 4.978
Thurlkill 4.609
EMBOSS 4.596
Sillero 4.876
Patrickios 4.279
IPC_peptide 4.711
IPC2_peptide 4.876
IPC2.peptide.svr19 4.829
Protein with the highest isoelectric point:
>tr|K4HJF7|K4HJF7_ARV Isoform of K4HKI5 Alpha 2 protein OS=Adelaide River virus OX=31612 GN=alpha PE=4 SV=1
MM1 pKa = 7.9 ADD3 pKa = 3.31 HH4 pKa = 7.08 ARR6 pKa = 11.84 LISLPSSVQDD16 pKa = 3.38 FFNQISSIFKK26 pKa = 10.25 SIEE29 pKa = 3.77 NFYY32 pKa = 11.17 LLYY35 pKa = 9.07 KK36 pKa = 9.9 TRR38 pKa = 11.84 LKK40 pKa = 10.62 IFLICTIALIVIVLVAKK57 pKa = 10.32 IIKK60 pKa = 8.21 YY61 pKa = 7.96 TLAFHH66 pKa = 6.95 ACIKK70 pKa = 10.08 IYY72 pKa = 10.27 CKK74 pKa = 10.31 PLIKK78 pKa = 8.49 TTNCLLKK85 pKa = 10.45 LGKK88 pKa = 9.8 RR89 pKa = 11.84 KK90 pKa = 9.46 RR91 pKa = 11.84 RR92 pKa = 11.84 KK93 pKa = 9.4 KK94 pKa = 9.47 KK95 pKa = 10.32 RR96 pKa = 11.84 KK97 pKa = 8.98 GKK99 pKa = 9.7 SIRR102 pKa = 11.84 LKK104 pKa = 10.41 EE105 pKa = 3.98 LSSS108 pKa = 3.53
Molecular weight: 12.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.252
IPC2_protein 9.78
IPC_protein 9.867
Toseland 10.716
ProMoST 10.204
Dawson 10.789
Bjellqvist 10.394
Wikipedia 10.906
Rodwell 11.433
Grimsley 10.818
Solomon 10.833
Lehninger 10.818
Nozaki 10.687
DTASelect 10.394
Thurlkill 10.687
EMBOSS 11.082
Sillero 10.701
Patrickios 11.169
IPC_peptide 10.833
IPC2_peptide 9.194
IPC2.peptide.svr19 8.576
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
1
9
4719
100
2144
524.3
60.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.437 ± 0.265
1.759 ± 0.303
5.912 ± 0.421
6.887 ± 0.655
4.153 ± 0.203
5.043 ± 0.226
2.352 ± 0.252
9.176 ± 0.441
8.646 ± 0.604
9.833 ± 0.651
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.67 ± 0.472
7.438 ± 0.482
3.285 ± 0.166
2.882 ± 0.256
4.471 ± 0.269
7.819 ± 0.544
4.641 ± 0.574
4.62 ± 0.45
1.441 ± 0.257
4.535 ± 0.333
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here