Pseudooceanicola flagellatus
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4189 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W2EB30|A0A1W2EB30_9RHOB 2-keto-myo-inositol isomerase OS=Pseudooceanicola flagellatus OX=1387277 GN=SAMN06295998_12520 PE=4 SV=1
MM1 pKa = 7.1 SASRR5 pKa = 11.84 LTLAAALAASTAIAAPAFAGSLDD28 pKa = 3.78 NTLSEE33 pKa = 4.89 PEE35 pKa = 3.77 PAAPVAVVAPAPLYY49 pKa = 10.7 DD50 pKa = 3.34 WTGPSIGAQLGFGDD64 pKa = 5.01 IEE66 pKa = 4.36 TEE68 pKa = 3.86 NAGGVEE74 pKa = 4.26 GDD76 pKa = 3.18 GGLYY80 pKa = 10.49 GLRR83 pKa = 11.84 AYY85 pKa = 10.93 YY86 pKa = 10.44 DD87 pKa = 3.79 YY88 pKa = 11.75 DD89 pKa = 3.38 FGNYY93 pKa = 7.29 VVGGGIQYY101 pKa = 10.51 DD102 pKa = 3.88 EE103 pKa = 4.92 SDD105 pKa = 3.23 IDD107 pKa = 3.48 IDD109 pKa = 4.83 GAATLDD115 pKa = 3.39 KK116 pKa = 10.94 VFRR119 pKa = 11.84 AGLRR123 pKa = 11.84 AGVDD127 pKa = 3.58 LNRR130 pKa = 11.84 TFLYY134 pKa = 9.07 GTGGYY139 pKa = 10.42 AKK141 pKa = 10.69 AFTEE145 pKa = 4.5 DD146 pKa = 3.85 DD147 pKa = 3.84 AVSLGDD153 pKa = 3.56 SNGYY157 pKa = 9.28 FVGLGAEE164 pKa = 4.21 HH165 pKa = 7.15 FLTEE169 pKa = 4.0 KK170 pKa = 9.43 VTVGGEE176 pKa = 3.82 ILYY179 pKa = 9.64 HH180 pKa = 6.06 QFDD183 pKa = 4.85 DD184 pKa = 4.64 FDD186 pKa = 4.96 ISDD189 pKa = 3.89 VEE191 pKa = 4.64 ANATTANVSVNYY203 pKa = 10.29 RR204 pKa = 11.84 FF205 pKa = 3.5
Molecular weight: 21.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.821
IPC_protein 3.808
Toseland 3.592
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.745
Rodwell 3.643
Grimsley 3.503
Solomon 3.795
Lehninger 3.757
Nozaki 3.923
DTASelect 4.164
Thurlkill 3.656
EMBOSS 3.757
Sillero 3.935
Patrickios 0.769
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.82
Protein with the highest isoelectric point:
>tr|A0A1W1ZNB3|A0A1W1ZNB3_9RHOB Acetylornithine aminotransferase OS=Pseudooceanicola flagellatus OX=1387277 GN=argD PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 AQGRR39 pKa = 11.84 KK40 pKa = 9.34 SLSAA44 pKa = 3.83
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4189
0
4189
1299626
29
2149
310.2
33.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.888 ± 0.048
0.93 ± 0.012
6.237 ± 0.031
5.802 ± 0.039
3.74 ± 0.024
8.517 ± 0.038
2.087 ± 0.018
5.257 ± 0.029
3.297 ± 0.032
9.982 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.817 ± 0.018
2.733 ± 0.021
4.967 ± 0.024
3.291 ± 0.022
6.711 ± 0.038
5.347 ± 0.028
5.565 ± 0.026
7.193 ± 0.034
1.389 ± 0.016
2.249 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here