Wenzhou tombus-like virus 8
Average proteome isoelectric point is 8.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KFP2|A0A1L3KFP2_9VIRU Uncharacterized protein OS=Wenzhou tombus-like virus 8 OX=1923678 PE=4 SV=1
MM1 pKa = 6.61 TTAKK5 pKa = 9.92 NHH7 pKa = 4.8 KK8 pKa = 8.68 TKK10 pKa = 10.41 TKK12 pKa = 9.42 NKK14 pKa = 9.79 RR15 pKa = 11.84 MNKK18 pKa = 6.68 TSKK21 pKa = 8.73 PARR24 pKa = 11.84 RR25 pKa = 11.84 PRR27 pKa = 11.84 VAKK30 pKa = 10.45 RR31 pKa = 11.84 NALTPVEE38 pKa = 4.99 RR39 pKa = 11.84 MIADD43 pKa = 4.04 PCHH46 pKa = 6.53 GPIEE50 pKa = 4.57 PIYY53 pKa = 9.62 TSEE56 pKa = 4.17 FGGGTVLKK64 pKa = 10.32 VRR66 pKa = 11.84 RR67 pKa = 11.84 RR68 pKa = 11.84 VPIHH72 pKa = 6.75 DD73 pKa = 4.48 LSSARR78 pKa = 11.84 NGFLLWYY85 pKa = 9.06 PDD87 pKa = 3.85 YY88 pKa = 11.83 ANGGQDD94 pKa = 4.59 GLGTTQGKK102 pKa = 7.86 SQPVRR107 pKa = 11.84 AGGANLFLYY116 pKa = 9.97 EE117 pKa = 5.18 AEE119 pKa = 5.03 DD120 pKa = 3.97 PSIPPTNSSAAGGYY134 pKa = 10.04 FGTGVNAQSPEE145 pKa = 4.3 GVSLPDD151 pKa = 3.57 PGFDD155 pKa = 3.55 IGINNNVSGMRR166 pKa = 11.84 TLGACMRR173 pKa = 11.84 IIYY176 pKa = 8.35 TGRR179 pKa = 11.84 ADD181 pKa = 3.47 FRR183 pKa = 11.84 SGEE186 pKa = 4.08 FVAVHH191 pKa = 6.78 RR192 pKa = 11.84 LPSSGLVNTEE202 pKa = 4.03 GAVTSPADD210 pKa = 3.89 LLTYY214 pKa = 10.38 GYY216 pKa = 8.63 DD217 pKa = 3.38 TFRR220 pKa = 11.84 TKK222 pKa = 10.7 DD223 pKa = 3.27 KK224 pKa = 10.86 EE225 pKa = 4.56 LIWRR229 pKa = 11.84 PGSNSEE235 pKa = 4.19 HH236 pKa = 6.24 PRR238 pKa = 11.84 KK239 pKa = 9.88 PGTLAFGSVSQEE251 pKa = 3.64 GVEE254 pKa = 5.2 DD255 pKa = 3.99 SFSDD259 pKa = 3.34 SAFRR263 pKa = 11.84 VGYY266 pKa = 7.9 NTQPTKK272 pKa = 10.83 VSNDD276 pKa = 3.49 SQPGEE281 pKa = 3.87 SSGFGFAWFNLPANASSDD299 pKa = 3.75 VYY301 pKa = 11.08 IEE303 pKa = 3.62 ITKK306 pKa = 9.99 IVQFQFSPINRR317 pKa = 11.84 TMEE320 pKa = 3.92 PVPYY324 pKa = 10.16 QIRR327 pKa = 11.84 DD328 pKa = 3.26 KK329 pKa = 11.07 AAQQTPDD336 pKa = 3.29 EE337 pKa = 4.54 AVAGVDD343 pKa = 3.92 RR344 pKa = 11.84 KK345 pKa = 10.65 CGPGWSLRR353 pKa = 11.84 DD354 pKa = 3.22 IGSYY358 pKa = 9.86 AQQASDD364 pKa = 4.34 MIQDD368 pKa = 3.53 VALGGLQLANTYY380 pKa = 10.83 HH381 pKa = 6.11 MVRR384 pKa = 11.84 NRR386 pKa = 11.84 SLRR389 pKa = 11.84 GAGQQDD395 pKa = 4.21 YY396 pKa = 11.69 LMVV399 pKa = 3.88
Molecular weight: 43.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.472
IPC2_protein 8.434
IPC_protein 8.404
Toseland 8.843
ProMoST 8.799
Dawson 9.209
Bjellqvist 9.077
Wikipedia 9.385
Rodwell 9.311
Grimsley 9.238
Solomon 9.297
Lehninger 9.253
Nozaki 9.092
DTASelect 9.004
Thurlkill 9.063
EMBOSS 9.326
Sillero 9.238
Patrickios 4.609
IPC_peptide 9.282
IPC2_peptide 7.805
IPC2.peptide.svr19 7.688
Protein with the highest isoelectric point:
>tr|A0A1L3KFP2|A0A1L3KFP2_9VIRU Uncharacterized protein OS=Wenzhou tombus-like virus 8 OX=1923678 PE=4 SV=1
MM1 pKa = 7.44 RR2 pKa = 11.84 QLNRR6 pKa = 11.84 LSLTMATTIRR16 pKa = 11.84 SLRR19 pKa = 11.84 SSAFTDD25 pKa = 3.65 HH26 pKa = 7.43 GDD28 pKa = 3.56 LQVNFDD34 pKa = 3.75 LRR36 pKa = 11.84 KK37 pKa = 9.75 AVLTLTSPVWAVPYY51 pKa = 10.8 GLFYY55 pKa = 10.6 CARR58 pKa = 11.84 RR59 pKa = 11.84 TAMCCFGSVIDD70 pKa = 5.22 KK71 pKa = 10.69 EE72 pKa = 4.37 SDD74 pKa = 3.99 DD75 pKa = 4.66 IISDD79 pKa = 3.77 LLEE82 pKa = 4.79 GYY84 pKa = 10.16 EE85 pKa = 3.94 EE86 pKa = 4.7 FINRR90 pKa = 11.84 DD91 pKa = 2.99 EE92 pKa = 5.26 HH93 pKa = 7.96 ADD95 pKa = 3.36 AMQADD100 pKa = 4.84 LEE102 pKa = 4.7 RR103 pKa = 11.84 INKK106 pKa = 9.02 VNEE109 pKa = 3.85 DD110 pKa = 3.21 FRR112 pKa = 11.84 KK113 pKa = 10.24 EE114 pKa = 3.84 KK115 pKa = 9.95 EE116 pKa = 4.06 AHH118 pKa = 6.39 DD119 pKa = 4.09 KK120 pKa = 11.1 AVASQDD126 pKa = 3.22 ATDD129 pKa = 3.51 EE130 pKa = 4.06 LTRR133 pKa = 11.84 NILALGSRR141 pKa = 11.84 PVMPPLPVGATDD153 pKa = 3.42 KK154 pKa = 10.97 DD155 pKa = 3.91 VRR157 pKa = 11.84 KK158 pKa = 8.75 LTTLYY163 pKa = 10.34 NYY165 pKa = 10.28 RR166 pKa = 11.84 RR167 pKa = 11.84 DD168 pKa = 3.8 RR169 pKa = 11.84 YY170 pKa = 10.12 DD171 pKa = 3.36 SQVTALKK178 pKa = 10.03 EE179 pKa = 3.84 LSANTLALNHH189 pKa = 6.15 NNAVDD194 pKa = 4.35 LVAPRR199 pKa = 11.84 PPEE202 pKa = 3.67 IRR204 pKa = 11.84 EE205 pKa = 3.83 GAIQRR210 pKa = 11.84 EE211 pKa = 4.11 KK212 pKa = 10.34 RR213 pKa = 11.84 LAMEE217 pKa = 4.27 EE218 pKa = 3.85 EE219 pKa = 4.23 TRR221 pKa = 11.84 PNRR224 pKa = 11.84 TRR226 pKa = 11.84 NKK228 pKa = 8.69 KK229 pKa = 8.81 RR230 pKa = 11.84 KK231 pKa = 8.7 FRR233 pKa = 11.84 RR234 pKa = 11.84 THH236 pKa = 6.12 EE237 pKa = 4.13 GLFLIVSYY245 pKa = 10.79 VKK247 pKa = 10.65 AIVPVEE253 pKa = 4.12 SDD255 pKa = 3.39 RR256 pKa = 11.84 KK257 pKa = 9.01 SQAITEE263 pKa = 4.53 SVRR266 pKa = 11.84 EE267 pKa = 4.14 LCRR270 pKa = 11.84 KK271 pKa = 9.61 AMQEE275 pKa = 4.12 RR276 pKa = 11.84 NWRR279 pKa = 11.84 LTDD282 pKa = 3.23 IAKK285 pKa = 8.93 WLPIATEE292 pKa = 4.05 MVRR295 pKa = 11.84 TPTEE299 pKa = 3.49 ADD301 pKa = 2.67 IMAKK305 pKa = 10.14 KK306 pKa = 9.9 FAQSSQGAQQHH317 pKa = 5.66 ARR319 pKa = 11.84 LKK321 pKa = 10.99 AFGGVPRR328 pKa = 11.84 CXGCACKK335 pKa = 10.25 ALSEE339 pKa = 4.38 EE340 pKa = 4.34 YY341 pKa = 10.02 HH342 pKa = 6.18 GKK344 pKa = 8.95 EE345 pKa = 3.76 LEE347 pKa = 4.39 YY348 pKa = 10.99 VGSDD352 pKa = 4.69 FMMKK356 pKa = 10.3 DD357 pKa = 3.21 PKK359 pKa = 11.1 LLGKK363 pKa = 10.01 HH364 pKa = 5.01 SLKK367 pKa = 10.45 VVPCMRR373 pKa = 11.84 VPKK376 pKa = 9.94 PRR378 pKa = 11.84 LLTRR382 pKa = 11.84 FTEE385 pKa = 4.6 AGSGARR391 pKa = 11.84 KK392 pKa = 9.26 VVYY395 pKa = 10.2 HH396 pKa = 5.87 NSVRR400 pKa = 11.84 NLVASLVGRR409 pKa = 11.84 VFLVKK414 pKa = 10.33 DD415 pKa = 3.59 GKK417 pKa = 10.93 GGFMPPPGPRR427 pKa = 11.84 NLNSLDD433 pKa = 3.35 KK434 pKa = 10.49 TLNKK438 pKa = 9.48 LWRR441 pKa = 11.84 YY442 pKa = 7.03 VHH444 pKa = 6.42 VGPPLSTSGFIGKK457 pKa = 8.13 YY458 pKa = 8.41 TGPKK462 pKa = 8.29 KK463 pKa = 10.22 AAYY466 pKa = 7.11 EE467 pKa = 3.85 QQGDD471 pKa = 3.44 IFHH474 pKa = 6.36 VVEE477 pKa = 4.05 TTRR480 pKa = 11.84 RR481 pKa = 11.84 DD482 pKa = 3.14 RR483 pKa = 11.84 RR484 pKa = 11.84 TEE486 pKa = 3.89 SFIKK490 pKa = 10.66 AEE492 pKa = 4.29 LMDD495 pKa = 4.39 SNYY498 pKa = 10.44 DD499 pKa = 3.33 RR500 pKa = 11.84 SSGQWEE506 pKa = 5.01 GKK508 pKa = 9.18 CPRR511 pKa = 11.84 NIRR514 pKa = 11.84 PMSIRR519 pKa = 11.84 VNLEE523 pKa = 3.69 LGLLIQPLEE532 pKa = 3.98 HH533 pKa = 6.06 AVYY536 pKa = 10.45 RR537 pKa = 11.84 GIDD540 pKa = 3.09 KK541 pKa = 10.39 MMRR544 pKa = 11.84 DD545 pKa = 4.35 LGCVRR550 pKa = 11.84 RR551 pKa = 11.84 TVTKK555 pKa = 10.26 GLNSFEE561 pKa = 4.18 MGEE564 pKa = 4.57 LIHH567 pKa = 6.28 QKK569 pKa = 8.02 WEE571 pKa = 4.0 RR572 pKa = 11.84 FVDD575 pKa = 4.52 PVCVQLDD582 pKa = 3.79 CEE584 pKa = 4.31 RR585 pKa = 11.84 FSQHH589 pKa = 5.37 VSYY592 pKa = 10.78 DD593 pKa = 3.08 AHH595 pKa = 6.69 KK596 pKa = 10.67 KK597 pKa = 7.83 LTRR600 pKa = 11.84 FLSSCYY606 pKa = 10.1 GGKK609 pKa = 8.22 KK610 pKa = 5.89 THH612 pKa = 6.62 NGRR615 pKa = 11.84 KK616 pKa = 9.56 LYY618 pKa = 9.85 TLLKK622 pKa = 8.5 YY623 pKa = 10.04 QWEE626 pKa = 4.69 TEE628 pKa = 4.23 LKK630 pKa = 9.23 TVLPDD635 pKa = 4.67 GIVTALIHH643 pKa = 5.81 GTLSDD648 pKa = 3.52 GVMNTSLYY656 pKa = 9.85 GILMMFLMVYY666 pKa = 9.56 EE667 pKa = 4.37 AVRR670 pKa = 11.84 TIRR673 pKa = 11.84 LTNFEE678 pKa = 4.61 IFDD681 pKa = 4.35 GGDD684 pKa = 3.2 DD685 pKa = 3.67 TNIIMEE691 pKa = 4.9 RR692 pKa = 11.84 KK693 pKa = 10.27 DD694 pKa = 2.67 KK695 pKa = 11.23 DD696 pKa = 3.59 AFVKK700 pKa = 9.62 TIQEE704 pKa = 4.16 VGVDD708 pKa = 3.3 AGFKK712 pKa = 10.31 IKK714 pKa = 10.73 FEE716 pKa = 4.21 GLSEE720 pKa = 4.21 SMEE723 pKa = 5.01 DD724 pKa = 4.03 IVFCRR729 pKa = 11.84 MNPVFDD735 pKa = 3.68 GARR738 pKa = 11.84 WRR740 pKa = 11.84 MVRR743 pKa = 11.84 NVEE746 pKa = 4.0 GALTRR751 pKa = 11.84 DD752 pKa = 3.37 VLTTKK757 pKa = 10.48 AVPNEE762 pKa = 3.94 EE763 pKa = 4.06 MFDD766 pKa = 3.34 ALRR769 pKa = 11.84 RR770 pKa = 11.84 AKK772 pKa = 10.21 AQSGLSLCFGMPVMQNFYY790 pKa = 11.46 EE791 pKa = 4.06 MLQRR795 pKa = 11.84 GTHH798 pKa = 5.59 PGKK801 pKa = 10.55 YY802 pKa = 9.29 RR803 pKa = 11.84 IVEE806 pKa = 4.22 EE807 pKa = 4.05 RR808 pKa = 11.84 SGMAFLAARR817 pKa = 11.84 LKK819 pKa = 10.78 PKK821 pKa = 8.83 TAPITEE827 pKa = 4.33 AARR830 pKa = 11.84 MSFWKK835 pKa = 10.6 ASGILPHH842 pKa = 6.42 KK843 pKa = 9.35 QILMEE848 pKa = 4.22 RR849 pKa = 11.84 EE850 pKa = 3.79 YY851 pKa = 11.77 DD852 pKa = 3.58 MMQPSYY858 pKa = 11.42 ARR860 pKa = 11.84 RR861 pKa = 11.84 CEE863 pKa = 3.46 ASFYY867 pKa = 9.92 HH868 pKa = 6.37 TFPEE872 pKa = 4.73 TTTVHH877 pKa = 6.97 HH878 pKa = 7.42 DD879 pKa = 3.45 YY880 pKa = 11.37 CC881 pKa = 6.72
Molecular weight: 100.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.691
IPC2_protein 8.536
IPC_protein 8.404
Toseland 9.063
ProMoST 8.975
Dawson 9.414
Bjellqvist 9.282
Wikipedia 9.56
Rodwell 9.619
Grimsley 9.428
Solomon 9.443
Lehninger 9.414
Nozaki 9.443
DTASelect 9.165
Thurlkill 9.311
EMBOSS 9.56
Sillero 9.458
Patrickios 4.914
IPC_peptide 9.443
IPC2_peptide 7.995
IPC2.peptide.svr19 7.729
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1280
399
881
640.0
71.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.344 ± 0.324
1.563 ± 0.389
5.547 ± 0.016
5.938 ± 0.924
4.063 ± 0.025
7.5 ± 1.451
2.188 ± 0.328
4.219 ± 0.02
5.938 ± 0.804
7.969 ± 1.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.438 ± 0.567
4.063 ± 0.576
5.234 ± 0.855
3.438 ± 0.515
8.047 ± 0.614
6.328 ± 0.931
6.25 ± 0.128
6.641 ± 0.18
1.016 ± 0.006
3.203 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here