Wenzhou tombus-like virus 8

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KFP2|A0A1L3KFP2_9VIRU Uncharacterized protein OS=Wenzhou tombus-like virus 8 OX=1923678 PE=4 SV=1
MM1 pKa = 6.61TTAKK5 pKa = 9.92NHH7 pKa = 4.8KK8 pKa = 8.68TKK10 pKa = 10.41TKK12 pKa = 9.42NKK14 pKa = 9.79RR15 pKa = 11.84MNKK18 pKa = 6.68TSKK21 pKa = 8.73PARR24 pKa = 11.84RR25 pKa = 11.84PRR27 pKa = 11.84VAKK30 pKa = 10.45RR31 pKa = 11.84NALTPVEE38 pKa = 4.99RR39 pKa = 11.84MIADD43 pKa = 4.04PCHH46 pKa = 6.53GPIEE50 pKa = 4.57PIYY53 pKa = 9.62TSEE56 pKa = 4.17FGGGTVLKK64 pKa = 10.32VRR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84VPIHH72 pKa = 6.75DD73 pKa = 4.48LSSARR78 pKa = 11.84NGFLLWYY85 pKa = 9.06PDD87 pKa = 3.85YY88 pKa = 11.83ANGGQDD94 pKa = 4.59GLGTTQGKK102 pKa = 7.86SQPVRR107 pKa = 11.84AGGANLFLYY116 pKa = 9.97EE117 pKa = 5.18AEE119 pKa = 5.03DD120 pKa = 3.97PSIPPTNSSAAGGYY134 pKa = 10.04FGTGVNAQSPEE145 pKa = 4.3GVSLPDD151 pKa = 3.57PGFDD155 pKa = 3.55IGINNNVSGMRR166 pKa = 11.84TLGACMRR173 pKa = 11.84IIYY176 pKa = 8.35TGRR179 pKa = 11.84ADD181 pKa = 3.47FRR183 pKa = 11.84SGEE186 pKa = 4.08FVAVHH191 pKa = 6.78RR192 pKa = 11.84LPSSGLVNTEE202 pKa = 4.03GAVTSPADD210 pKa = 3.89LLTYY214 pKa = 10.38GYY216 pKa = 8.63DD217 pKa = 3.38TFRR220 pKa = 11.84TKK222 pKa = 10.7DD223 pKa = 3.27KK224 pKa = 10.86EE225 pKa = 4.56LIWRR229 pKa = 11.84PGSNSEE235 pKa = 4.19HH236 pKa = 6.24PRR238 pKa = 11.84KK239 pKa = 9.88PGTLAFGSVSQEE251 pKa = 3.64GVEE254 pKa = 5.2DD255 pKa = 3.99SFSDD259 pKa = 3.34SAFRR263 pKa = 11.84VGYY266 pKa = 7.9NTQPTKK272 pKa = 10.83VSNDD276 pKa = 3.49SQPGEE281 pKa = 3.87SSGFGFAWFNLPANASSDD299 pKa = 3.75VYY301 pKa = 11.08IEE303 pKa = 3.62ITKK306 pKa = 9.99IVQFQFSPINRR317 pKa = 11.84TMEE320 pKa = 3.92PVPYY324 pKa = 10.16QIRR327 pKa = 11.84DD328 pKa = 3.26KK329 pKa = 11.07AAQQTPDD336 pKa = 3.29EE337 pKa = 4.54AVAGVDD343 pKa = 3.92RR344 pKa = 11.84KK345 pKa = 10.65CGPGWSLRR353 pKa = 11.84DD354 pKa = 3.22IGSYY358 pKa = 9.86AQQASDD364 pKa = 4.34MIQDD368 pKa = 3.53VALGGLQLANTYY380 pKa = 10.83HH381 pKa = 6.11MVRR384 pKa = 11.84NRR386 pKa = 11.84SLRR389 pKa = 11.84GAGQQDD395 pKa = 4.21YY396 pKa = 11.69LMVV399 pKa = 3.88

Molecular weight:
43.31 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KFP2|A0A1L3KFP2_9VIRU Uncharacterized protein OS=Wenzhou tombus-like virus 8 OX=1923678 PE=4 SV=1
MM1 pKa = 7.44RR2 pKa = 11.84QLNRR6 pKa = 11.84LSLTMATTIRR16 pKa = 11.84SLRR19 pKa = 11.84SSAFTDD25 pKa = 3.65HH26 pKa = 7.43GDD28 pKa = 3.56LQVNFDD34 pKa = 3.75LRR36 pKa = 11.84KK37 pKa = 9.75AVLTLTSPVWAVPYY51 pKa = 10.8GLFYY55 pKa = 10.6CARR58 pKa = 11.84RR59 pKa = 11.84TAMCCFGSVIDD70 pKa = 5.22KK71 pKa = 10.69EE72 pKa = 4.37SDD74 pKa = 3.99DD75 pKa = 4.66IISDD79 pKa = 3.77LLEE82 pKa = 4.79GYY84 pKa = 10.16EE85 pKa = 3.94EE86 pKa = 4.7FINRR90 pKa = 11.84DD91 pKa = 2.99EE92 pKa = 5.26HH93 pKa = 7.96ADD95 pKa = 3.36AMQADD100 pKa = 4.84LEE102 pKa = 4.7RR103 pKa = 11.84INKK106 pKa = 9.02VNEE109 pKa = 3.85DD110 pKa = 3.21FRR112 pKa = 11.84KK113 pKa = 10.24EE114 pKa = 3.84KK115 pKa = 9.95EE116 pKa = 4.06AHH118 pKa = 6.39DD119 pKa = 4.09KK120 pKa = 11.1AVASQDD126 pKa = 3.22ATDD129 pKa = 3.51EE130 pKa = 4.06LTRR133 pKa = 11.84NILALGSRR141 pKa = 11.84PVMPPLPVGATDD153 pKa = 3.42KK154 pKa = 10.97DD155 pKa = 3.91VRR157 pKa = 11.84KK158 pKa = 8.75LTTLYY163 pKa = 10.34NYY165 pKa = 10.28RR166 pKa = 11.84RR167 pKa = 11.84DD168 pKa = 3.8RR169 pKa = 11.84YY170 pKa = 10.12DD171 pKa = 3.36SQVTALKK178 pKa = 10.03EE179 pKa = 3.84LSANTLALNHH189 pKa = 6.15NNAVDD194 pKa = 4.35LVAPRR199 pKa = 11.84PPEE202 pKa = 3.67IRR204 pKa = 11.84EE205 pKa = 3.83GAIQRR210 pKa = 11.84EE211 pKa = 4.11KK212 pKa = 10.34RR213 pKa = 11.84LAMEE217 pKa = 4.27EE218 pKa = 3.85EE219 pKa = 4.23TRR221 pKa = 11.84PNRR224 pKa = 11.84TRR226 pKa = 11.84NKK228 pKa = 8.69KK229 pKa = 8.81RR230 pKa = 11.84KK231 pKa = 8.7FRR233 pKa = 11.84RR234 pKa = 11.84THH236 pKa = 6.12EE237 pKa = 4.13GLFLIVSYY245 pKa = 10.79VKK247 pKa = 10.65AIVPVEE253 pKa = 4.12SDD255 pKa = 3.39RR256 pKa = 11.84KK257 pKa = 9.01SQAITEE263 pKa = 4.53SVRR266 pKa = 11.84EE267 pKa = 4.14LCRR270 pKa = 11.84KK271 pKa = 9.61AMQEE275 pKa = 4.12RR276 pKa = 11.84NWRR279 pKa = 11.84LTDD282 pKa = 3.23IAKK285 pKa = 8.93WLPIATEE292 pKa = 4.05MVRR295 pKa = 11.84TPTEE299 pKa = 3.49ADD301 pKa = 2.67IMAKK305 pKa = 10.14KK306 pKa = 9.9FAQSSQGAQQHH317 pKa = 5.66ARR319 pKa = 11.84LKK321 pKa = 10.99AFGGVPRR328 pKa = 11.84CXGCACKK335 pKa = 10.25ALSEE339 pKa = 4.38EE340 pKa = 4.34YY341 pKa = 10.02HH342 pKa = 6.18GKK344 pKa = 8.95EE345 pKa = 3.76LEE347 pKa = 4.39YY348 pKa = 10.99VGSDD352 pKa = 4.69FMMKK356 pKa = 10.3DD357 pKa = 3.21PKK359 pKa = 11.1LLGKK363 pKa = 10.01HH364 pKa = 5.01SLKK367 pKa = 10.45VVPCMRR373 pKa = 11.84VPKK376 pKa = 9.94PRR378 pKa = 11.84LLTRR382 pKa = 11.84FTEE385 pKa = 4.6AGSGARR391 pKa = 11.84KK392 pKa = 9.26VVYY395 pKa = 10.2HH396 pKa = 5.87NSVRR400 pKa = 11.84NLVASLVGRR409 pKa = 11.84VFLVKK414 pKa = 10.33DD415 pKa = 3.59GKK417 pKa = 10.93GGFMPPPGPRR427 pKa = 11.84NLNSLDD433 pKa = 3.35KK434 pKa = 10.49TLNKK438 pKa = 9.48LWRR441 pKa = 11.84YY442 pKa = 7.03VHH444 pKa = 6.42VGPPLSTSGFIGKK457 pKa = 8.13YY458 pKa = 8.41TGPKK462 pKa = 8.29KK463 pKa = 10.22AAYY466 pKa = 7.11EE467 pKa = 3.85QQGDD471 pKa = 3.44IFHH474 pKa = 6.36VVEE477 pKa = 4.05TTRR480 pKa = 11.84RR481 pKa = 11.84DD482 pKa = 3.14RR483 pKa = 11.84RR484 pKa = 11.84TEE486 pKa = 3.89SFIKK490 pKa = 10.66AEE492 pKa = 4.29LMDD495 pKa = 4.39SNYY498 pKa = 10.44DD499 pKa = 3.33RR500 pKa = 11.84SSGQWEE506 pKa = 5.01GKK508 pKa = 9.18CPRR511 pKa = 11.84NIRR514 pKa = 11.84PMSIRR519 pKa = 11.84VNLEE523 pKa = 3.69LGLLIQPLEE532 pKa = 3.98HH533 pKa = 6.06AVYY536 pKa = 10.45RR537 pKa = 11.84GIDD540 pKa = 3.09KK541 pKa = 10.39MMRR544 pKa = 11.84DD545 pKa = 4.35LGCVRR550 pKa = 11.84RR551 pKa = 11.84TVTKK555 pKa = 10.26GLNSFEE561 pKa = 4.18MGEE564 pKa = 4.57LIHH567 pKa = 6.28QKK569 pKa = 8.02WEE571 pKa = 4.0RR572 pKa = 11.84FVDD575 pKa = 4.52PVCVQLDD582 pKa = 3.79CEE584 pKa = 4.31RR585 pKa = 11.84FSQHH589 pKa = 5.37VSYY592 pKa = 10.78DD593 pKa = 3.08AHH595 pKa = 6.69KK596 pKa = 10.67KK597 pKa = 7.83LTRR600 pKa = 11.84FLSSCYY606 pKa = 10.1GGKK609 pKa = 8.22KK610 pKa = 5.89THH612 pKa = 6.62NGRR615 pKa = 11.84KK616 pKa = 9.56LYY618 pKa = 9.85TLLKK622 pKa = 8.5YY623 pKa = 10.04QWEE626 pKa = 4.69TEE628 pKa = 4.23LKK630 pKa = 9.23TVLPDD635 pKa = 4.67GIVTALIHH643 pKa = 5.81GTLSDD648 pKa = 3.52GVMNTSLYY656 pKa = 9.85GILMMFLMVYY666 pKa = 9.56EE667 pKa = 4.37AVRR670 pKa = 11.84TIRR673 pKa = 11.84LTNFEE678 pKa = 4.61IFDD681 pKa = 4.35GGDD684 pKa = 3.2DD685 pKa = 3.67TNIIMEE691 pKa = 4.9RR692 pKa = 11.84KK693 pKa = 10.27DD694 pKa = 2.67KK695 pKa = 11.23DD696 pKa = 3.59AFVKK700 pKa = 9.62TIQEE704 pKa = 4.16VGVDD708 pKa = 3.3AGFKK712 pKa = 10.31IKK714 pKa = 10.73FEE716 pKa = 4.21GLSEE720 pKa = 4.21SMEE723 pKa = 5.01DD724 pKa = 4.03IVFCRR729 pKa = 11.84MNPVFDD735 pKa = 3.68GARR738 pKa = 11.84WRR740 pKa = 11.84MVRR743 pKa = 11.84NVEE746 pKa = 4.0GALTRR751 pKa = 11.84DD752 pKa = 3.37VLTTKK757 pKa = 10.48AVPNEE762 pKa = 3.94EE763 pKa = 4.06MFDD766 pKa = 3.34ALRR769 pKa = 11.84RR770 pKa = 11.84AKK772 pKa = 10.21AQSGLSLCFGMPVMQNFYY790 pKa = 11.46EE791 pKa = 4.06MLQRR795 pKa = 11.84GTHH798 pKa = 5.59PGKK801 pKa = 10.55YY802 pKa = 9.29RR803 pKa = 11.84IVEE806 pKa = 4.22EE807 pKa = 4.05RR808 pKa = 11.84SGMAFLAARR817 pKa = 11.84LKK819 pKa = 10.78PKK821 pKa = 8.83TAPITEE827 pKa = 4.33AARR830 pKa = 11.84MSFWKK835 pKa = 10.6ASGILPHH842 pKa = 6.42KK843 pKa = 9.35QILMEE848 pKa = 4.22RR849 pKa = 11.84EE850 pKa = 3.79YY851 pKa = 11.77DD852 pKa = 3.58MMQPSYY858 pKa = 11.42ARR860 pKa = 11.84RR861 pKa = 11.84CEE863 pKa = 3.46ASFYY867 pKa = 9.92HH868 pKa = 6.37TFPEE872 pKa = 4.73TTTVHH877 pKa = 6.97HH878 pKa = 7.42DD879 pKa = 3.45YY880 pKa = 11.37CC881 pKa = 6.72

Molecular weight:
100.44 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1280

399

881

640.0

71.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.344 ± 0.324

1.563 ± 0.389

5.547 ± 0.016

5.938 ± 0.924

4.063 ± 0.025

7.5 ± 1.451

2.188 ± 0.328

4.219 ± 0.02

5.938 ± 0.804

7.969 ± 1.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.438 ± 0.567

4.063 ± 0.576

5.234 ± 0.855

3.438 ± 0.515

8.047 ± 0.614

6.328 ± 0.931

6.25 ± 0.128

6.641 ± 0.18

1.016 ± 0.006

3.203 ± 0.147

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski