Vibrio diazotrophicus

Taxonomy: cellular organisms; Bacteria; Proteobacteria;

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4064 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2J8GUC2|A0A2J8GUC2_VIBDI Methyl-accepting chemotaxis protein OS=Vibrio diazotrophicus OX=685 GN=C1M56_06225 PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 9.08KK3 pKa = 8.28TLLALAVLAAAGSAQAGIEE22 pKa = 3.97IYY24 pKa = 10.69NNEE27 pKa = 3.99GVTVNLKK34 pKa = 10.5GDD36 pKa = 3.61IEE38 pKa = 4.05ITYY41 pKa = 10.62QNSTDD46 pKa = 3.8SASMSQQIEE55 pKa = 4.04DD56 pKa = 3.71ADD58 pKa = 3.96FGFDD62 pKa = 3.78VRR64 pKa = 11.84YY65 pKa = 10.73AMNDD69 pKa = 3.21QVSIGAYY76 pKa = 8.96WEE78 pKa = 4.53FNGSDD83 pKa = 3.64TNNATVTKK91 pKa = 10.91NGDD94 pKa = 3.48NYY96 pKa = 10.21VALYY100 pKa = 8.89TADD103 pKa = 3.45FGSIKK108 pKa = 10.38FGRR111 pKa = 11.84LCTAIDD117 pKa = 4.97DD118 pKa = 3.91IGIGNDD124 pKa = 3.29YY125 pKa = 11.01QFGINSFFDD134 pKa = 3.51KK135 pKa = 10.27KK136 pKa = 9.4TFEE139 pKa = 4.54CQDD142 pKa = 2.94EE143 pKa = 4.35AVRR146 pKa = 11.84YY147 pKa = 10.0DD148 pKa = 3.68YY149 pKa = 11.67DD150 pKa = 3.32NGMFYY155 pKa = 10.31ATLGYY160 pKa = 9.45VQNKK164 pKa = 9.67LNDD167 pKa = 3.15GSTDD171 pKa = 3.52YY172 pKa = 10.36TAGGAEE178 pKa = 5.16DD179 pKa = 3.27IAANQGSDD187 pKa = 2.93ADD189 pKa = 4.37YY190 pKa = 10.65IDD192 pKa = 5.03GYY194 pKa = 10.8VGARR198 pKa = 11.84FAGFDD203 pKa = 3.63AKK205 pKa = 10.96VILADD210 pKa = 3.74YY211 pKa = 11.11DD212 pKa = 3.95SDD214 pKa = 4.1NDD216 pKa = 3.9AAPLSSSLLGLEE228 pKa = 4.09VAYY231 pKa = 10.53GGVEE235 pKa = 3.93NLNLSVGYY243 pKa = 9.96YY244 pKa = 10.33SADD247 pKa = 3.31KK248 pKa = 11.34DD249 pKa = 3.72EE250 pKa = 5.63AGTNKK255 pKa = 10.69DD256 pKa = 2.98NDD258 pKa = 3.75TWALAADD265 pKa = 3.74YY266 pKa = 9.27TIGKK270 pKa = 8.73VVVGAGYY277 pKa = 9.79SQTEE281 pKa = 3.95FDD283 pKa = 5.33ASDD286 pKa = 3.61KK287 pKa = 10.63EE288 pKa = 4.25QTNSFVNVAYY298 pKa = 9.73KK299 pKa = 10.24VLPAVKK305 pKa = 10.23VYY307 pKa = 11.38GEE309 pKa = 4.01VAKK312 pKa = 10.52RR313 pKa = 11.84DD314 pKa = 3.61LSGTGVTAFEE324 pKa = 4.44NEE326 pKa = 3.42NLAAVGVTASFF337 pKa = 3.6

Molecular weight: 36.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2J8I7P1|A0A2J8I7P1_VIBDI Nitrogen fixation protein NifT OS=Vibrio diazotrophicus OX=685 GN=nifT PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 8.99RR4 pKa = 11.84TFQPTVLKK12 pKa = 10.46RR13 pKa = 11.84KK14 pKa = 7.65RR15 pKa = 11.84THH17 pKa = 5.89GFRR20 pKa = 11.84ARR22 pKa = 11.84MATANGRR29 pKa = 11.84KK30 pKa = 9.34VINARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.74GRR40 pKa = 11.84KK41 pKa = 8.91RR42 pKa = 11.84LSKK45 pKa = 10.84

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4064

0

4064

1322842

24

3331

325.5

36.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.478 ± 0.039

1.039 ± 0.014

5.366 ± 0.031

6.456 ± 0.041

4.112 ± 0.028

6.726 ± 0.033

2.249 ± 0.018

6.446 ± 0.031

5.432 ± 0.031

10.327 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.713 ± 0.019

4.285 ± 0.027

3.821 ± 0.027

4.523 ± 0.031

4.478 ± 0.033

6.753 ± 0.038

5.278 ± 0.032

7.176 ± 0.034

1.264 ± 0.018

3.075 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski