Sinimarinibacterium flocculans
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4018 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A318EDB5|A0A318EDB5_9GAMM Cytochrome P450 OS=Sinimarinibacterium flocculans OX=985250 GN=C8D93_102340 PE=3 SV=1
MM1 pKa = 7.35 RR2 pKa = 11.84 TLANLGVVLALAAPCSAFAGHH23 pKa = 6.13 QSNLDD28 pKa = 3.29 GYY30 pKa = 9.56 YY31 pKa = 9.69 IPQSEE36 pKa = 4.5 FSFEE40 pKa = 4.28 PEE42 pKa = 3.4 TALPFDD48 pKa = 4.38 FDD50 pKa = 4.71 EE51 pKa = 5.28 NGDD54 pKa = 3.73 GYY56 pKa = 10.88 GIKK59 pKa = 10.42 AQFQVSSVFFIHH71 pKa = 7.33 GEE73 pKa = 4.04 YY74 pKa = 10.05 QAIDD78 pKa = 3.69 YY79 pKa = 11.41 GEE81 pKa = 5.21 DD82 pKa = 3.74 DD83 pKa = 3.86 DD84 pKa = 5.4 TGVLKK89 pKa = 10.74 DD90 pKa = 4.23 DD91 pKa = 4.58 LNTYY95 pKa = 10.27 RR96 pKa = 11.84 IGGGFFFSDD105 pKa = 3.46 SQPFFVKK112 pKa = 10.7 GEE114 pKa = 4.18 YY115 pKa = 9.74 IGVDD119 pKa = 3.73 LGDD122 pKa = 4.13 SDD124 pKa = 6.29 DD125 pKa = 4.17 GDD127 pKa = 3.91 EE128 pKa = 5.91 EE129 pKa = 4.85 IDD131 pKa = 3.57 EE132 pKa = 5.1 DD133 pKa = 4.7 YY134 pKa = 11.37 NGYY137 pKa = 9.51 GVHH140 pKa = 6.55 IGALGHH146 pKa = 6.9 VGDD149 pKa = 5.0 ALNLTASIGYY159 pKa = 9.71 VDD161 pKa = 5.14 LDD163 pKa = 3.55 GTAGIEE169 pKa = 4.02 FLVGVGFQLTPLIGVFADD187 pKa = 3.58 YY188 pKa = 11.1 RR189 pKa = 11.84 NTDD192 pKa = 3.39 LEE194 pKa = 5.13 LDD196 pKa = 4.03 DD197 pKa = 5.46 GGGDD201 pKa = 3.76 FKK203 pKa = 11.21 LTDD206 pKa = 3.01 VRR208 pKa = 11.84 TGVRR212 pKa = 11.84 FVFF215 pKa = 4.26
Molecular weight: 23.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.704
IPC2_protein 3.694
IPC_protein 3.719
Toseland 3.49
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.668
Rodwell 3.541
Grimsley 3.389
Solomon 3.706
Lehninger 3.668
Nozaki 3.834
DTASelect 4.101
Thurlkill 3.541
EMBOSS 3.681
Sillero 3.834
Patrickios 0.846
IPC_peptide 3.706
IPC2_peptide 3.808
IPC2.peptide.svr19 3.756
Protein with the highest isoelectric point:
>tr|A0A318E3N4|A0A318E3N4_9GAMM RadC-like JAB domain-containing protein (Fragment) OS=Sinimarinibacterium flocculans OX=985250 GN=C8D93_1231 PE=4 SV=1
MM1 pKa = 7.74 SKK3 pKa = 8.96 RR4 pKa = 11.84 TYY6 pKa = 9.58 QPKK9 pKa = 9.34 KK10 pKa = 9.24 LRR12 pKa = 11.84 RR13 pKa = 11.84 KK14 pKa = 7.83 RR15 pKa = 11.84 THH17 pKa = 5.96 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.34 GGRR29 pKa = 11.84 LVLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.93 GRR40 pKa = 11.84 KK41 pKa = 8.79 RR42 pKa = 11.84 LAPP45 pKa = 3.99
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.45
IPC2_protein 11.169
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.427
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.149
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.074
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4018
0
4018
1348336
27
2842
335.6
36.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.784 ± 0.052
0.972 ± 0.013
6.004 ± 0.031
5.607 ± 0.031
3.446 ± 0.02
8.503 ± 0.035
2.186 ± 0.02
4.304 ± 0.024
2.505 ± 0.031
10.759 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.138 ± 0.019
2.338 ± 0.023
5.449 ± 0.034
3.605 ± 0.02
8.09 ± 0.049
4.978 ± 0.027
4.99 ± 0.029
7.437 ± 0.033
1.484 ± 0.017
2.419 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here