Amino acid dipepetide frequency for Microbacterium phage OneinaGillian

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.119AlaAla: 15.119 ± 1.218
0.913AlaCys: 0.913 ± 0.233
7.204AlaAsp: 7.204 ± 0.89
7.965AlaGlu: 7.965 ± 0.817
2.588AlaPhe: 2.588 ± 0.44
8.727AlaGly: 8.727 ± 0.761
2.283AlaHis: 2.283 ± 0.335
5.023AlaIle: 5.023 ± 0.629
2.993AlaLys: 2.993 ± 0.415
9.792AlaLeu: 9.792 ± 0.866
3.095AlaMet: 3.095 ± 0.429
3.501AlaAsn: 3.501 ± 0.5
4.972AlaPro: 4.972 ± 0.625
4.11AlaGln: 4.11 ± 0.604
6.646AlaArg: 6.646 ± 0.598
7.204AlaSer: 7.204 ± 0.64
7.61AlaThr: 7.61 ± 0.992
8.067AlaVal: 8.067 ± 0.823
1.674AlaTrp: 1.674 ± 0.293
2.79AlaTyr: 2.79 ± 0.457
0.0AlaXaa: 0.0 ± 0.0
Cys
0.609CysAla: 0.609 ± 0.206
0.152CysCys: 0.152 ± 0.095
0.609CysAsp: 0.609 ± 0.167
0.71CysGlu: 0.71 ± 0.18
0.254CysPhe: 0.254 ± 0.136
1.116CysGly: 1.116 ± 0.251
0.355CysHis: 0.355 ± 0.157
0.355CysIle: 0.355 ± 0.152
0.203CysLys: 0.203 ± 0.101
0.406CysLeu: 0.406 ± 0.173
0.101CysMet: 0.101 ± 0.081
0.304CysAsn: 0.304 ± 0.113
0.761CysPro: 0.761 ± 0.204
0.203CysGln: 0.203 ± 0.112
0.71CysArg: 0.71 ± 0.199
0.457CysSer: 0.457 ± 0.217
0.457CysThr: 0.457 ± 0.159
0.507CysVal: 0.507 ± 0.164
0.304CysTrp: 0.304 ± 0.121
0.203CysTyr: 0.203 ± 0.114
0.0CysXaa: 0.0 ± 0.0
Asp
7.661AspAla: 7.661 ± 0.7
0.507AspCys: 0.507 ± 0.156
4.566AspAsp: 4.566 ± 0.594
4.668AspGlu: 4.668 ± 0.614
2.029AspPhe: 2.029 ± 0.304
6.494AspGly: 6.494 ± 0.657
1.116AspHis: 1.116 ± 0.294
3.196AspIle: 3.196 ± 0.432
1.421AspLys: 1.421 ± 0.322
4.921AspLeu: 4.921 ± 0.731
1.522AspMet: 1.522 ± 0.324
1.725AspAsn: 1.725 ± 0.292
3.45AspPro: 3.45 ± 0.414
2.182AspGln: 2.182 ± 0.406
3.856AspArg: 3.856 ± 0.455
3.044AspSer: 3.044 ± 0.413
2.486AspThr: 2.486 ± 0.342
3.805AspVal: 3.805 ± 0.421
1.624AspTrp: 1.624 ± 0.254
1.674AspTyr: 1.674 ± 0.416
0.0AspXaa: 0.0 ± 0.0
Glu
7.509GluAla: 7.509 ± 0.92
0.609GluCys: 0.609 ± 0.184
4.262GluAsp: 4.262 ± 0.578
6.19GluGlu: 6.19 ± 0.866
2.182GluPhe: 2.182 ± 0.355
4.668GluGly: 4.668 ± 0.621
1.826GluHis: 1.826 ± 0.351
4.11GluIle: 4.11 ± 0.48
2.537GluLys: 2.537 ± 0.494
6.748GluLeu: 6.748 ± 0.823
1.573GluMet: 1.573 ± 0.292
1.776GluAsn: 1.776 ± 0.316
3.805GluPro: 3.805 ± 0.724
3.501GluGln: 3.501 ± 0.478
4.972GluArg: 4.972 ± 0.593
3.805GluSer: 3.805 ± 0.468
3.45GluThr: 3.45 ± 0.502
5.327GluVal: 5.327 ± 0.478
1.37GluTrp: 1.37 ± 0.22
2.435GluTyr: 2.435 ± 0.334
0.0GluXaa: 0.0 ± 0.0
Phe
3.196PheAla: 3.196 ± 0.49
0.203PheCys: 0.203 ± 0.107
2.232PheAsp: 2.232 ± 0.417
1.979PheGlu: 1.979 ± 0.363
0.761PhePhe: 0.761 ± 0.159
3.856PheGly: 3.856 ± 0.56
0.355PheHis: 0.355 ± 0.134
0.812PheIle: 0.812 ± 0.188
0.812PheLys: 0.812 ± 0.244
1.776PheLeu: 1.776 ± 0.372
0.507PheMet: 0.507 ± 0.17
0.355PheAsn: 0.355 ± 0.156
1.218PhePro: 1.218 ± 0.209
0.558PheGln: 0.558 ± 0.167
1.928PheArg: 1.928 ± 0.249
2.029PheSer: 2.029 ± 0.282
2.334PheThr: 2.334 ± 0.391
1.624PheVal: 1.624 ± 0.273
0.406PheTrp: 0.406 ± 0.153
0.863PheTyr: 0.863 ± 0.198
0.0PheXaa: 0.0 ± 0.0
Gly
8.98GlyAla: 8.98 ± 0.91
0.863GlyCys: 0.863 ± 0.299
5.987GlyAsp: 5.987 ± 0.549
5.023GlyGlu: 5.023 ± 0.597
2.892GlyPhe: 2.892 ± 0.394
9.234GlyGly: 9.234 ± 0.782
1.37GlyHis: 1.37 ± 0.293
3.551GlyIle: 3.551 ± 0.362
3.653GlyLys: 3.653 ± 0.698
5.632GlyLeu: 5.632 ± 0.623
2.182GlyMet: 2.182 ± 0.298
2.841GlyAsn: 2.841 ± 0.393
2.993GlyPro: 2.993 ± 0.438
2.79GlyGln: 2.79 ± 0.491
5.733GlyArg: 5.733 ± 0.6
5.277GlySer: 5.277 ± 0.617
6.646GlyThr: 6.646 ± 0.908
6.596GlyVal: 6.596 ± 0.445
1.674GlyTrp: 1.674 ± 0.311
3.146GlyTyr: 3.146 ± 0.348
0.0GlyXaa: 0.0 ± 0.0
His
2.029HisAla: 2.029 ± 0.326
0.304HisCys: 0.304 ± 0.146
0.863HisAsp: 0.863 ± 0.23
1.776HisGlu: 1.776 ± 0.354
0.457HisPhe: 0.457 ± 0.134
1.116HisGly: 1.116 ± 0.225
0.457HisHis: 0.457 ± 0.154
0.71HisIle: 0.71 ± 0.216
0.355HisLys: 0.355 ± 0.127
1.319HisLeu: 1.319 ± 0.235
0.355HisMet: 0.355 ± 0.159
0.558HisAsn: 0.558 ± 0.151
1.573HisPro: 1.573 ± 0.368
0.609HisGln: 0.609 ± 0.172
1.116HisArg: 1.116 ± 0.287
0.761HisSer: 0.761 ± 0.191
1.065HisThr: 1.065 ± 0.215
1.674HisVal: 1.674 ± 0.32
0.406HisTrp: 0.406 ± 0.14
0.406HisTyr: 0.406 ± 0.151
0.0HisXaa: 0.0 ± 0.0
Ile
5.733IleAla: 5.733 ± 0.664
0.355IleCys: 0.355 ± 0.134
3.856IleAsp: 3.856 ± 0.378
4.465IleGlu: 4.465 ± 0.495
1.37IlePhe: 1.37 ± 0.255
3.907IleGly: 3.907 ± 0.528
0.609IleHis: 0.609 ± 0.184
2.334IleIle: 2.334 ± 0.383
1.065IleLys: 1.065 ± 0.241
3.298IleLeu: 3.298 ± 0.436
0.609IleMet: 0.609 ± 0.172
1.471IleAsn: 1.471 ± 0.273
3.754IlePro: 3.754 ± 0.603
1.573IleGln: 1.573 ± 0.287
3.298IleArg: 3.298 ± 0.377
2.79IleSer: 2.79 ± 0.395
3.602IleThr: 3.602 ± 0.373
3.957IleVal: 3.957 ± 0.443
0.609IleTrp: 0.609 ± 0.188
0.964IleTyr: 0.964 ± 0.226
0.0IleXaa: 0.0 ± 0.0
Lys
3.653LysAla: 3.653 ± 0.534
0.254LysCys: 0.254 ± 0.149
0.913LysAsp: 0.913 ± 0.224
2.283LysGlu: 2.283 ± 0.454
0.863LysPhe: 0.863 ± 0.212
2.486LysGly: 2.486 ± 0.419
0.863LysHis: 0.863 ± 0.218
1.826LysIle: 1.826 ± 0.254
1.725LysLys: 1.725 ± 0.283
2.74LysLeu: 2.74 ± 0.38
0.964LysMet: 0.964 ± 0.27
0.863LysAsn: 0.863 ± 0.205
1.471LysPro: 1.471 ± 0.337
0.863LysGln: 0.863 ± 0.212
2.08LysArg: 2.08 ± 0.372
2.131LysSer: 2.131 ± 0.427
1.979LysThr: 1.979 ± 0.314
2.232LysVal: 2.232 ± 0.411
0.558LysTrp: 0.558 ± 0.176
0.66LysTyr: 0.66 ± 0.183
0.0LysXaa: 0.0 ± 0.0
Leu
9.691LeuAla: 9.691 ± 0.949
0.457LeuCys: 0.457 ± 0.156
5.327LeuAsp: 5.327 ± 0.534
5.936LeuGlu: 5.936 ± 0.619
1.725LeuPhe: 1.725 ± 0.366
6.748LeuGly: 6.748 ± 0.646
1.268LeuHis: 1.268 ± 0.257
3.653LeuIle: 3.653 ± 0.53
2.435LeuLys: 2.435 ± 0.35
5.733LeuLeu: 5.733 ± 0.689
1.776LeuMet: 1.776 ± 0.327
2.638LeuAsn: 2.638 ± 0.417
3.45LeuPro: 3.45 ± 0.507
1.573LeuGln: 1.573 ± 0.338
6.19LeuArg: 6.19 ± 0.572
5.023LeuSer: 5.023 ± 0.601
6.545LeuThr: 6.545 ± 0.678
4.668LeuVal: 4.668 ± 0.546
0.863LeuTrp: 0.863 ± 0.233
1.674LeuTyr: 1.674 ± 0.261
0.0LeuXaa: 0.0 ± 0.0
Met
2.588MetAla: 2.588 ± 0.387
0.152MetCys: 0.152 ± 0.078
0.964MetAsp: 0.964 ± 0.258
0.863MetGlu: 0.863 ± 0.21
0.457MetPhe: 0.457 ± 0.165
1.674MetGly: 1.674 ± 0.323
0.507MetHis: 0.507 ± 0.176
1.319MetIle: 1.319 ± 0.299
0.812MetLys: 0.812 ± 0.198
2.182MetLeu: 2.182 ± 0.325
0.355MetMet: 0.355 ± 0.141
1.015MetAsn: 1.015 ± 0.25
1.471MetPro: 1.471 ± 0.265
0.507MetGln: 0.507 ± 0.122
2.029MetArg: 2.029 ± 0.337
2.334MetSer: 2.334 ± 0.387
1.776MetThr: 1.776 ± 0.374
1.573MetVal: 1.573 ± 0.304
0.254MetTrp: 0.254 ± 0.103
0.457MetTyr: 0.457 ± 0.18
0.0MetXaa: 0.0 ± 0.0
Asn
2.892AsnAla: 2.892 ± 0.376
0.152AsnCys: 0.152 ± 0.098
2.029AsnAsp: 2.029 ± 0.35
1.776AsnGlu: 1.776 ± 0.337
0.71AsnPhe: 0.71 ± 0.241
3.349AsnGly: 3.349 ± 0.486
0.203AsnHis: 0.203 ± 0.098
1.065AsnIle: 1.065 ± 0.211
0.558AsnLys: 0.558 ± 0.152
2.08AsnLeu: 2.08 ± 0.488
0.406AsnMet: 0.406 ± 0.123
0.609AsnAsn: 0.609 ± 0.161
2.486AsnPro: 2.486 ± 0.458
1.015AsnGln: 1.015 ± 0.213
1.776AsnArg: 1.776 ± 0.321
1.522AsnSer: 1.522 ± 0.293
2.029AsnThr: 2.029 ± 0.358
1.928AsnVal: 1.928 ± 0.304
0.406AsnTrp: 0.406 ± 0.154
1.065AsnTyr: 1.065 ± 0.213
0.0AsnXaa: 0.0 ± 0.0
Pro
6.799ProAla: 6.799 ± 1.718
0.457ProCys: 0.457 ± 0.141
3.399ProAsp: 3.399 ± 0.476
4.972ProGlu: 4.972 ± 0.698
0.964ProPhe: 0.964 ± 0.246
4.921ProGly: 4.921 ± 0.554
0.609ProHis: 0.609 ± 0.17
2.334ProIle: 2.334 ± 0.336
1.421ProLys: 1.421 ± 0.328
3.957ProLeu: 3.957 ± 0.451
1.015ProMet: 1.015 ± 0.21
1.674ProAsn: 1.674 ± 0.271
3.095ProPro: 3.095 ± 0.411
2.131ProGln: 2.131 ± 0.6
2.74ProArg: 2.74 ± 0.387
2.841ProSer: 2.841 ± 0.333
4.262ProThr: 4.262 ± 0.609
4.211ProVal: 4.211 ± 0.447
1.116ProTrp: 1.116 ± 0.226
1.37ProTyr: 1.37 ± 0.312
0.0ProXaa: 0.0 ± 0.0
Gln
4.059GlnAla: 4.059 ± 0.501
0.152GlnCys: 0.152 ± 0.099
0.812GlnAsp: 0.812 ± 0.208
1.776GlnGlu: 1.776 ± 0.422
0.964GlnPhe: 0.964 ± 0.272
2.74GlnGly: 2.74 ± 0.591
0.609GlnHis: 0.609 ± 0.182
1.928GlnIle: 1.928 ± 0.354
0.913GlnLys: 0.913 ± 0.231
2.486GlnLeu: 2.486 ± 0.374
1.167GlnMet: 1.167 ± 0.265
1.319GlnAsn: 1.319 ± 0.26
1.979GlnPro: 1.979 ± 0.553
2.74GlnGln: 2.74 ± 1.01
2.79GlnArg: 2.79 ± 0.381
1.725GlnSer: 1.725 ± 0.255
2.182GlnThr: 2.182 ± 0.34
2.131GlnVal: 2.131 ± 0.374
0.71GlnTrp: 0.71 ± 0.168
0.71GlnTyr: 0.71 ± 0.218
0.0GlnXaa: 0.0 ± 0.0
Arg
6.342ArgAla: 6.342 ± 0.529
0.863ArgCys: 0.863 ± 0.245
3.602ArgAsp: 3.602 ± 0.514
5.023ArgGlu: 5.023 ± 0.606
2.486ArgPhe: 2.486 ± 0.44
5.175ArgGly: 5.175 ± 0.547
1.167ArgHis: 1.167 ± 0.266
3.602ArgIle: 3.602 ± 0.479
2.841ArgLys: 2.841 ± 0.51
5.175ArgLeu: 5.175 ± 0.5
2.638ArgMet: 2.638 ± 0.367
1.776ArgAsn: 1.776 ± 0.338
3.247ArgPro: 3.247 ± 0.451
2.486ArgGln: 2.486 ± 0.352
5.733ArgArg: 5.733 ± 0.664
3.45ArgSer: 3.45 ± 0.427
4.414ArgThr: 4.414 ± 0.418
5.074ArgVal: 5.074 ± 0.518
1.471ArgTrp: 1.471 ± 0.308
1.573ArgTyr: 1.573 ± 0.242
0.0ArgXaa: 0.0 ± 0.0
Ser
6.24SerAla: 6.24 ± 0.919
0.863SerCys: 0.863 ± 0.267
3.501SerAsp: 3.501 ± 0.395
3.602SerGlu: 3.602 ± 0.38
1.624SerPhe: 1.624 ± 0.266
4.515SerGly: 4.515 ± 0.558
0.913SerHis: 0.913 ± 0.25
2.892SerIle: 2.892 ± 0.433
2.029SerLys: 2.029 ± 0.32
5.175SerLeu: 5.175 ± 0.504
1.218SerMet: 1.218 ± 0.28
1.471SerAsn: 1.471 ± 0.339
3.298SerPro: 3.298 ± 0.381
1.725SerGln: 1.725 ± 0.3
4.262SerArg: 4.262 ± 0.41
4.465SerSer: 4.465 ± 0.737
5.023SerThr: 5.023 ± 0.577
4.414SerVal: 4.414 ± 0.515
0.66SerTrp: 0.66 ± 0.184
1.218SerTyr: 1.218 ± 0.26
0.0SerXaa: 0.0 ± 0.0
Thr
6.596ThrAla: 6.596 ± 0.878
0.406ThrCys: 0.406 ± 0.148
3.957ThrAsp: 3.957 ± 0.532
5.277ThrGlu: 5.277 ± 0.58
2.385ThrPhe: 2.385 ± 0.43
6.443ThrGly: 6.443 ± 0.627
1.268ThrHis: 1.268 ± 0.233
4.668ThrIle: 4.668 ± 0.621
2.232ThrLys: 2.232 ± 0.305
5.53ThrLeu: 5.53 ± 0.675
1.471ThrMet: 1.471 ± 0.263
1.471ThrAsn: 1.471 ± 0.295
4.972ThrPro: 4.972 ± 0.914
1.979ThrGln: 1.979 ± 0.392
4.515ThrArg: 4.515 ± 0.593
4.059ThrSer: 4.059 ± 0.626
4.668ThrThr: 4.668 ± 0.694
5.175ThrVal: 5.175 ± 0.631
1.725ThrTrp: 1.725 ± 0.29
1.421ThrTyr: 1.421 ± 0.329
0.0ThrXaa: 0.0 ± 0.0
Val
7.813ValAla: 7.813 ± 0.744
0.71ValCys: 0.71 ± 0.161
4.617ValAsp: 4.617 ± 0.474
5.124ValGlu: 5.124 ± 0.565
1.877ValPhe: 1.877 ± 0.317
5.835ValGly: 5.835 ± 0.584
1.471ValHis: 1.471 ± 0.251
4.059ValIle: 4.059 ± 0.372
2.029ValLys: 2.029 ± 0.36
5.581ValLeu: 5.581 ± 0.619
1.471ValMet: 1.471 ± 0.251
2.029ValAsn: 2.029 ± 0.322
4.313ValPro: 4.313 ± 0.772
2.232ValGln: 2.232 ± 0.29
4.465ValArg: 4.465 ± 0.467
3.907ValSer: 3.907 ± 0.492
5.581ValThr: 5.581 ± 0.645
5.733ValVal: 5.733 ± 0.428
1.877ValTrp: 1.877 ± 0.316
1.522ValTyr: 1.522 ± 0.267
0.0ValXaa: 0.0 ± 0.0
Trp
1.979TrpAla: 1.979 ± 0.323
0.152TrpCys: 0.152 ± 0.087
1.116TrpAsp: 1.116 ± 0.179
1.218TrpGlu: 1.218 ± 0.279
0.66TrpPhe: 0.66 ± 0.174
1.167TrpGly: 1.167 ± 0.219
0.406TrpHis: 0.406 ± 0.139
1.065TrpIle: 1.065 ± 0.299
0.66TrpLys: 0.66 ± 0.164
1.522TrpLeu: 1.522 ± 0.3
0.355TrpMet: 0.355 ± 0.124
0.203TrpAsn: 0.203 ± 0.089
0.761TrpPro: 0.761 ± 0.183
0.457TrpGln: 0.457 ± 0.159
1.522TrpArg: 1.522 ± 0.325
1.218TrpSer: 1.218 ± 0.25
2.08TrpThr: 2.08 ± 0.342
1.421TrpVal: 1.421 ± 0.254
0.609TrpTrp: 0.609 ± 0.255
0.406TrpTyr: 0.406 ± 0.135
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.79TyrAla: 2.79 ± 0.414
0.254TyrCys: 0.254 ± 0.122
2.131TyrAsp: 2.131 ± 0.386
2.029TyrGlu: 2.029 ± 0.347
0.558TyrPhe: 0.558 ± 0.174
2.892TyrGly: 2.892 ± 0.375
0.254TyrHis: 0.254 ± 0.119
1.116TyrIle: 1.116 ± 0.263
0.71TyrLys: 0.71 ± 0.26
1.319TyrLeu: 1.319 ± 0.267
0.457TyrMet: 0.457 ± 0.152
0.457TyrAsn: 0.457 ± 0.127
1.218TyrPro: 1.218 ± 0.257
0.66TyrGln: 0.66 ± 0.157
1.826TyrArg: 1.826 ± 0.333
1.065TyrSer: 1.065 ± 0.275
2.029TyrThr: 2.029 ± 0.416
2.131TyrVal: 2.131 ± 0.493
0.66TyrTrp: 0.66 ± 0.219
0.406TyrTyr: 0.406 ± 0.161
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 103 proteins (19711 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski