Mumps virus (strain Miyahara vaccine) (MuV)
Average proteome isoelectric point is 7.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P22112|SH_MUMPM Small hydrophobic protein OS=Mumps virus (strain Miyahara vaccine) OX=11171 GN=SH PE=1 SV=1
MM1 pKa = 7.49 SSVLKK6 pKa = 10.65 AFEE9 pKa = 4.31 RR10 pKa = 11.84 FTIEE14 pKa = 5.13 QEE16 pKa = 3.95 LQDD19 pKa = 3.9 RR20 pKa = 11.84 GEE22 pKa = 4.45 EE23 pKa = 4.26 GSIPPEE29 pKa = 4.03 TLKK32 pKa = 11.08 SAVKK36 pKa = 10.23 VFVINTPNPTTRR48 pKa = 11.84 YY49 pKa = 10.18 HH50 pKa = 6.21 MLNFCLRR57 pKa = 11.84 IICSQNARR65 pKa = 11.84 ASHH68 pKa = 6.07 RR69 pKa = 11.84 VGALITLFSLPSAGMQNHH87 pKa = 6.05 IRR89 pKa = 11.84 LADD92 pKa = 4.27 RR93 pKa = 11.84 SPEE96 pKa = 3.9 AQIEE100 pKa = 4.18 RR101 pKa = 11.84 CEE103 pKa = 4.02 IDD105 pKa = 3.34 GFEE108 pKa = 4.47 PGTYY112 pKa = 10.03 RR113 pKa = 11.84 LIPNARR119 pKa = 11.84 ANLTANEE126 pKa = 3.72 IAAYY130 pKa = 10.25 ALLADD135 pKa = 5.13 DD136 pKa = 6.14 LPPTINNGTPYY147 pKa = 10.34 VHH149 pKa = 7.43 ADD151 pKa = 3.72 VEE153 pKa = 4.47 GQPCDD158 pKa = 3.97 EE159 pKa = 4.8 IEE161 pKa = 4.09 QFLDD165 pKa = 2.71 RR166 pKa = 11.84 CYY168 pKa = 11.21 SVLIQAWVMVCKK180 pKa = 10.67 CMTAYY185 pKa = 9.79 DD186 pKa = 4.06 QPAGSADD193 pKa = 3.45 RR194 pKa = 11.84 RR195 pKa = 11.84 FAKK198 pKa = 10.22 YY199 pKa = 8.47 QQQGRR204 pKa = 11.84 LEE206 pKa = 4.21 ARR208 pKa = 11.84 YY209 pKa = 8.28 MLQPEE214 pKa = 4.46 AQRR217 pKa = 11.84 LIQTAIRR224 pKa = 11.84 KK225 pKa = 9.17 SLVVRR230 pKa = 11.84 QYY232 pKa = 10.98 LTFEE236 pKa = 4.12 LQLARR241 pKa = 11.84 RR242 pKa = 11.84 QGLLSNRR249 pKa = 11.84 YY250 pKa = 8.0 YY251 pKa = 11.82 AMVGDD256 pKa = 3.41 IGKK259 pKa = 10.26 YY260 pKa = 9.45 IEE262 pKa = 4.47 NSGLTAFFLTLKK274 pKa = 10.27 YY275 pKa = 10.86 ALGTKK280 pKa = 9.03 WSPLSLAAFTGEE292 pKa = 4.05 LTKK295 pKa = 10.79 LRR297 pKa = 11.84 SLMMLYY303 pKa = 10.24 RR304 pKa = 11.84 DD305 pKa = 3.69 LGEE308 pKa = 3.91 QARR311 pKa = 11.84 YY312 pKa = 8.75 LALLEE317 pKa = 4.89 APQIMDD323 pKa = 4.55 FAPGGYY329 pKa = 9.29 PLIFSYY335 pKa = 11.38 AMGVGTVLDD344 pKa = 3.74 VQMRR348 pKa = 11.84 NYY350 pKa = 9.87 TYY352 pKa = 11.25 ARR354 pKa = 11.84 PFLNGYY360 pKa = 7.57 YY361 pKa = 9.8 FQIGVEE367 pKa = 4.14 TARR370 pKa = 11.84 RR371 pKa = 11.84 QQGTVDD377 pKa = 3.5 NRR379 pKa = 11.84 VADD382 pKa = 4.32 DD383 pKa = 4.52 LGLTPEE389 pKa = 3.96 QRR391 pKa = 11.84 TEE393 pKa = 3.78 VTQLIDD399 pKa = 2.97 RR400 pKa = 11.84 LARR403 pKa = 11.84 GRR405 pKa = 11.84 GAGIPGGPVNPFVPPVQQQQPAAAYY430 pKa = 9.13 EE431 pKa = 4.58 DD432 pKa = 3.88 IPALEE437 pKa = 4.64 EE438 pKa = 4.19 SDD440 pKa = 5.43 DD441 pKa = 5.79 DD442 pKa = 4.71 GDD444 pKa = 3.87 EE445 pKa = 4.51 DD446 pKa = 5.2 GGAGFQNGAQAPAARR461 pKa = 11.84 QGGQNDD467 pKa = 3.7 FRR469 pKa = 11.84 VQPLQDD475 pKa = 4.84 PIQAQLFMPLYY486 pKa = 8.72 PQVSNIPNHH495 pKa = 5.8 QNHH498 pKa = 6.27 QINRR502 pKa = 11.84 IGGMEE507 pKa = 4.03 HH508 pKa = 7.16 QDD510 pKa = 3.26 LLRR513 pKa = 11.84 YY514 pKa = 9.57 NEE516 pKa = 4.83 NGDD519 pKa = 3.68 SQQDD523 pKa = 3.16 ARR525 pKa = 11.84 GEE527 pKa = 4.14 HH528 pKa = 6.79 GNTFPNNPNQNAQSQVGDD546 pKa = 3.37 WDD548 pKa = 3.74 EE549 pKa = 3.83
Molecular weight: 61.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.014
IPC2_protein 5.105
IPC_protein 5.067
Toseland 5.003
ProMoST 5.143
Dawson 5.054
Bjellqvist 5.194
Wikipedia 4.927
Rodwell 4.965
Grimsley 4.927
Solomon 5.054
Lehninger 5.016
Nozaki 5.181
DTASelect 5.334
Thurlkill 5.003
EMBOSS 4.978
Sillero 5.245
Patrickios 3.821
IPC_peptide 5.067
IPC2_peptide 5.245
IPC2.peptide.svr19 5.192
Protein with the highest isoelectric point:
>sp|P30928|V_MUMPM Non-structural protein V OS=Mumps virus (strain Miyahara vaccine) OX=11171 GN=P/V PE=1 SV=1
MM1 pKa = 7.73 PAIQPPLYY9 pKa = 8.82 PTFLLLILLSLIITLYY25 pKa = 9.9 VWIISTITYY34 pKa = 6.96 KK35 pKa = 10.16 TAVRR39 pKa = 11.84 HH40 pKa = 5.43 AALHH44 pKa = 5.06 QRR46 pKa = 11.84 SFSRR50 pKa = 11.84 WSLDD54 pKa = 2.96 HH55 pKa = 6.98 SLL57 pKa = 4.1
Molecular weight: 6.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.043
IPC2_protein 9.97
IPC_protein 10.511
Toseland 9.823
ProMoST 9.823
Dawson 10.189
Bjellqvist 9.984
Wikipedia 10.467
Rodwell 10.248
Grimsley 10.321
Solomon 10.262
Lehninger 10.218
Nozaki 9.78
DTASelect 9.999
Thurlkill 9.97
EMBOSS 10.262
Sillero 10.087
Patrickios 7.497
IPC_peptide 10.248
IPC2_peptide 8.99
IPC2.peptide.svr19 8.391
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
4977
57
2261
622.1
69.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.631 ± 0.789
1.929 ± 0.292
4.561 ± 0.451
4.842 ± 0.571
3.355 ± 0.243
5.646 ± 1.046
1.869 ± 0.206
7.836 ± 0.78
4.26 ± 0.567
10.589 ± 1.373
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.311 ± 0.147
5.204 ± 0.532
5.365 ± 0.618
5.083 ± 0.815
5.083 ± 0.423
8.62 ± 0.792
6.409 ± 0.449
5.767 ± 0.353
1.125 ± 0.245
3.516 ± 0.387
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here