Escherichia phage ECBP2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Kuravirus; Escherichia virus ECB2

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 120 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J9SN50|J9SN50_9CAUD Cell adhesion domain protein OS=Escherichia phage ECBP2 OX=1604355 GN=ECBP2_0017 PE=4 SV=1
MM1 pKa = 7.31FLDD4 pKa = 4.83FLSEE8 pKa = 4.58LIPYY12 pKa = 9.35FRR14 pKa = 11.84TEE16 pKa = 3.37TCAFKK21 pKa = 11.13VEE23 pKa = 4.05EE24 pKa = 4.99DD25 pKa = 3.53GDD27 pKa = 4.38FEE29 pKa = 6.14VITCLGRR36 pKa = 11.84LVDD39 pKa = 5.64LDD41 pKa = 4.35DD42 pKa = 6.77DD43 pKa = 4.36EE44 pKa = 7.05DD45 pKa = 5.54FDD47 pKa = 4.27VTIDD51 pKa = 3.97LSSLGWLYY59 pKa = 10.59MRR61 pKa = 11.84FCITYY66 pKa = 10.01KK67 pKa = 10.35PEE69 pKa = 3.79TYY71 pKa = 10.19KK72 pKa = 10.78DD73 pKa = 3.45YY74 pKa = 11.03RR75 pKa = 11.84YY76 pKa = 6.83TTEE79 pKa = 4.24GEE81 pKa = 3.75NDD83 pKa = 3.24EE84 pKa = 4.41

Molecular weight: 10.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J9SGH3|J9SGH3_9CAUD Uncharacterized protein OS=Escherichia phage ECBP2 OX=1604355 GN=ECBP2_0078 PE=4 SV=1
MM1 pKa = 7.15KK2 pKa = 10.18RR3 pKa = 11.84RR4 pKa = 11.84WVVRR8 pKa = 11.84IHH10 pKa = 6.35WSSGTEE16 pKa = 3.72SMGIRR21 pKa = 11.84NTRR24 pKa = 11.84QEE26 pKa = 3.74ARR28 pKa = 11.84AHH30 pKa = 6.27KK31 pKa = 10.26KK32 pKa = 10.08FLLSCEE38 pKa = 4.04STINKK43 pKa = 7.69ITIHH47 pKa = 6.65KK48 pKa = 10.08AQDD51 pKa = 3.49FKK53 pKa = 11.44SGRR56 pKa = 11.84FYY58 pKa = 10.79ISGKK62 pKa = 7.3VHH64 pKa = 5.79YY65 pKa = 10.08

Molecular weight: 7.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

120

0

120

22737

38

1472

189.5

21.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.088 ± 0.531

1.126 ± 0.147

6.219 ± 0.19

6.94 ± 0.33

3.853 ± 0.197

7.107 ± 0.313

1.83 ± 0.147

5.656 ± 0.166

6.157 ± 0.286

7.362 ± 0.174

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.709 ± 0.116

5.467 ± 0.198

3.73 ± 0.16

4.077 ± 0.32

5.304 ± 0.244

6.848 ± 0.228

5.849 ± 0.314

6.65 ± 0.251

1.456 ± 0.109

3.571 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski