Halorubrum saccharovorum

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Halorubraceae; Halorubrum

Average proteome isoelectric point is 4.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2334 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A081EX71|A0A081EX71_9EURY Hydrolase OS=Halorubrum saccharovorum OX=2248 GN=FK85_11285 PE=4 SV=1
MM1 pKa = 7.7EE2 pKa = 5.18SPRR5 pKa = 11.84KK6 pKa = 8.47LTTLIIIAGMVALAGCTGVIPGDD29 pKa = 3.74PAPADD34 pKa = 3.65PEE36 pKa = 4.22PANGSSTVTTNGSVEE51 pKa = 3.71VHH53 pKa = 6.43YY54 pKa = 10.97INVGQSVSTLITSPNGDD71 pKa = 3.28TMLVDD76 pKa = 3.67TGHH79 pKa = 6.22YY80 pKa = 10.12NDD82 pKa = 5.41DD83 pKa = 4.08GEE85 pKa = 4.98HH86 pKa = 5.36VLAYY90 pKa = 9.61LQRR93 pKa = 11.84HH94 pKa = 6.22DD95 pKa = 3.99ISRR98 pKa = 11.84IDD100 pKa = 3.62HH101 pKa = 6.62LVTSHH106 pKa = 6.88SDD108 pKa = 3.1ADD110 pKa = 4.29HH111 pKa = 6.8IGGNAAIIKK120 pKa = 9.64YY121 pKa = 10.25YY122 pKa = 8.54EE123 pKa = 4.14TEE125 pKa = 3.79ADD127 pKa = 4.24GIGAVYY133 pKa = 10.42DD134 pKa = 3.92PGIASSTQTYY144 pKa = 10.78AEE146 pKa = 4.13YY147 pKa = 10.98LDD149 pKa = 5.56AIEE152 pKa = 4.53AHH154 pKa = 7.09DD155 pKa = 3.69VTLYY159 pKa = 7.32EE160 pKa = 4.17TRR162 pKa = 11.84EE163 pKa = 3.69GDD165 pKa = 3.64TIDD168 pKa = 4.74FGDD171 pKa = 3.6VDD173 pKa = 4.51VDD175 pKa = 3.79VLGPPDD181 pKa = 4.8PYY183 pKa = 11.22LEE185 pKa = 4.36SEE187 pKa = 4.43DD188 pKa = 4.4RR189 pKa = 11.84NEE191 pKa = 3.74NSIVLKK197 pKa = 9.16LTHH200 pKa = 6.83GEE202 pKa = 4.16TSFLLSGDD210 pKa = 4.15AEE212 pKa = 4.88DD213 pKa = 4.69DD214 pKa = 3.69QEE216 pKa = 6.29AYY218 pKa = 10.68LVDD221 pKa = 4.11EE222 pKa = 4.89YY223 pKa = 11.57GSEE226 pKa = 4.27LEE228 pKa = 4.18STILKK233 pKa = 10.17AGHH236 pKa = 6.74HH237 pKa = 6.51GSASSSSGAFLDD249 pKa = 4.76AVDD252 pKa = 4.16PQAVVISSAYY262 pKa = 9.72DD263 pKa = 3.14SQYY266 pKa = 10.55GHH268 pKa = 6.39PHH270 pKa = 6.01EE271 pKa = 4.92EE272 pKa = 3.94VLEE275 pKa = 4.11RR276 pKa = 11.84LAEE279 pKa = 4.25RR280 pKa = 11.84SHH282 pKa = 6.11PAYY285 pKa = 7.58WTATHH290 pKa = 6.39GNIVLTSDD298 pKa = 3.46GQTVTVQTQRR308 pKa = 11.84DD309 pKa = 4.15SPTDD313 pKa = 3.56PSTLRR318 pKa = 11.84EE319 pKa = 4.07GDD321 pKa = 4.28PIDD324 pKa = 4.01VGVPDD329 pKa = 4.34AVSDD333 pKa = 3.76RR334 pKa = 11.84ARR336 pKa = 11.84IEE338 pKa = 4.11SDD340 pKa = 2.8GTTSIDD346 pKa = 3.26TGSNEE351 pKa = 4.27TPSDD355 pKa = 3.67ADD357 pKa = 3.59SEE359 pKa = 4.49TDD361 pKa = 3.15EE362 pKa = 4.36TAAEE366 pKa = 4.26AGLTVAEE373 pKa = 4.35IHH375 pKa = 6.93ADD377 pKa = 3.3AAGDD381 pKa = 4.69DD382 pKa = 3.82RR383 pKa = 11.84DD384 pKa = 4.07NLNDD388 pKa = 3.53EE389 pKa = 4.46YY390 pKa = 11.73VVFEE394 pKa = 4.04NTGNEE399 pKa = 4.09TLDD402 pKa = 3.49LSGWTIEE409 pKa = 4.66DD410 pKa = 3.47EE411 pKa = 4.14VGQRR415 pKa = 11.84YY416 pKa = 6.41TVPEE420 pKa = 4.38GFEE423 pKa = 4.05LAPGQTVTLHH433 pKa = 6.02TGSGTSTTTEE443 pKa = 4.65LYY445 pKa = 8.1WASGSPIWNNDD456 pKa = 2.31GDD458 pKa = 4.18IVIVSNANGEE468 pKa = 4.33RR469 pKa = 11.84VLEE472 pKa = 4.13EE473 pKa = 4.32TYY475 pKa = 11.25SS476 pKa = 3.34

Molecular weight: 50.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F8BHS3|A0A0F8BHS3_9EURY NUDIX hydrolase OS=Halorubrum saccharovorum OX=2248 GN=FK85_25910 PE=4 SV=1
RR1 pKa = 7.3GRR3 pKa = 11.84HH4 pKa = 4.35QAGASATAPATARR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84GAGIARR26 pKa = 11.84RR27 pKa = 11.84APRR30 pKa = 11.84DD31 pKa = 2.9RR32 pKa = 11.84RR33 pKa = 11.84ARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84GRR39 pKa = 11.84VRR41 pKa = 11.84ASGRR45 pKa = 11.84PSPARR50 pKa = 11.84PRR52 pKa = 11.84RR53 pKa = 11.84PGRR56 pKa = 11.84PRR58 pKa = 11.84EE59 pKa = 3.87RR60 pKa = 11.84RR61 pKa = 3.39

Molecular weight: 6.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2334

0

2334

584998

29

1869

250.6

27.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.04 ± 0.081

0.66 ± 0.017

8.879 ± 0.067

8.719 ± 0.076

3.323 ± 0.034

8.811 ± 0.058

1.908 ± 0.027

4.151 ± 0.038

1.855 ± 0.037

8.641 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.745 ± 0.025

2.318 ± 0.031

4.628 ± 0.033

2.245 ± 0.035

6.68 ± 0.048

5.538 ± 0.039

6.118 ± 0.038

9.063 ± 0.057

1.083 ± 0.017

2.595 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski