Crocuta crocuta papillomavirus 1
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J7EZS5|J7EZS5_9PAPI Major capsid protein L1 OS=Crocuta crocuta papillomavirus 1 OX=1104917 GN=L1 PE=3 SV=1
MM1 pKa = 7.48 RR2 pKa = 11.84 GEE4 pKa = 4.3 RR5 pKa = 11.84 PTLQEE10 pKa = 3.59 IVLTEE15 pKa = 4.12 LPEE18 pKa = 5.85 HH19 pKa = 7.04 IDD21 pKa = 4.05 LEE23 pKa = 4.66 CHH25 pKa = 4.61 EE26 pKa = 4.4 QMPEE30 pKa = 3.87 EE31 pKa = 4.29 EE32 pKa = 4.68 EE33 pKa = 4.09 EE34 pKa = 4.22 EE35 pKa = 4.27 EE36 pKa = 4.94 PISRR40 pKa = 11.84 DD41 pKa = 3.27 CFRR44 pKa = 11.84 VATCCGYY51 pKa = 10.16 CRR53 pKa = 11.84 KK54 pKa = 9.42 EE55 pKa = 3.93 LRR57 pKa = 11.84 FLCLSSAAQVRR68 pKa = 11.84 SLEE71 pKa = 4.12 SLLLQDD77 pKa = 5.18 FSFICTSCVVKK88 pKa = 10.75 KK89 pKa = 10.51 KK90 pKa = 10.4 LNHH93 pKa = 6.32 GGG95 pKa = 3.38
Molecular weight: 10.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.824
IPC2_protein 4.978
IPC_protein 4.838
Toseland 4.749
ProMoST 4.927
Dawson 4.774
Bjellqvist 4.914
Wikipedia 4.584
Rodwell 4.711
Grimsley 4.66
Solomon 4.774
Lehninger 4.724
Nozaki 4.889
DTASelect 4.94
Thurlkill 4.736
EMBOSS 4.622
Sillero 4.965
Patrickios 3.478
IPC_peptide 4.787
IPC2_peptide 4.965
IPC2.peptide.svr19 4.944
Protein with the highest isoelectric point:
>tr|J7F282|J7F282_9PAPI Protein E6 OS=Crocuta crocuta papillomavirus 1 OX=1104917 GN=E6 PE=3 SV=1
MM1 pKa = 7.12 EE2 pKa = 4.97 TLSQLLEE9 pKa = 4.13 SLQNDD14 pKa = 3.62 MLTLYY19 pKa = 10.51 EE20 pKa = 4.64 KK21 pKa = 10.98 DD22 pKa = 3.32 SDD24 pKa = 4.03 RR25 pKa = 11.84 LTDD28 pKa = 5.5 QITHH32 pKa = 4.64 WHH34 pKa = 6.65 LNRR37 pKa = 11.84 KK38 pKa = 7.42 EE39 pKa = 3.61 QTLYY43 pKa = 10.55 YY44 pKa = 10.2 GARR47 pKa = 11.84 QRR49 pKa = 11.84 GITRR53 pKa = 11.84 IGYY56 pKa = 8.13 NHH58 pKa = 6.12 VPSLAASQQRR68 pKa = 11.84 AKK70 pKa = 10.5 QAIEE74 pKa = 3.67 QEE76 pKa = 4.49 LILRR80 pKa = 11.84 TLLDD84 pKa = 3.65 SEE86 pKa = 4.84 FGEE89 pKa = 4.61 EE90 pKa = 4.28 PWNLMQTSRR99 pKa = 11.84 EE100 pKa = 3.89 RR101 pKa = 11.84 LLAPPAYY108 pKa = 9.23 CFKK111 pKa = 10.91 KK112 pKa = 10.23 GGHH115 pKa = 6.32 NIDD118 pKa = 3.28 VKK120 pKa = 11.13 FNNDD124 pKa = 2.83 SQNIARR130 pKa = 11.84 YY131 pKa = 8.55 VLWDD135 pKa = 4.47 FIYY138 pKa = 11.14 YY139 pKa = 9.63 QDD141 pKa = 5.73 SEE143 pKa = 4.77 DD144 pKa = 3.04 KK145 pKa = 9.6 WRR147 pKa = 11.84 VSKK150 pKa = 11.04 GYY152 pKa = 10.45 VDD154 pKa = 3.56 EE155 pKa = 4.65 TGLYY159 pKa = 10.09 YY160 pKa = 9.91 EE161 pKa = 4.71 TEE163 pKa = 4.29 LGIKK167 pKa = 9.32 IYY169 pKa = 10.62 YY170 pKa = 10.12 VNFHH174 pKa = 7.38 DD175 pKa = 5.04 EE176 pKa = 3.7 AAKK179 pKa = 10.69 YY180 pKa = 10.31 GNSDD184 pKa = 3.02 VYY186 pKa = 11.04 EE187 pKa = 4.26 VVKK190 pKa = 10.14 TPLTTPVSTSTSGLNTASPGPHH212 pKa = 6.56 SDD214 pKa = 2.59 SGTPRR219 pKa = 11.84 RR220 pKa = 11.84 KK221 pKa = 8.24 TSTPVKK227 pKa = 9.95 SRR229 pKa = 11.84 KK230 pKa = 9.26 KK231 pKa = 9.82 SGRR234 pKa = 11.84 HH235 pKa = 5.54 ALPKK239 pKa = 9.37 TPVGRR244 pKa = 11.84 RR245 pKa = 11.84 FGRR248 pKa = 11.84 RR249 pKa = 11.84 KK250 pKa = 10.09 GEE252 pKa = 4.09 RR253 pKa = 11.84 VSTATPSSKK262 pKa = 10.3 PPSPEE267 pKa = 3.78 QIGSSHH273 pKa = 5.86 QSVPVRR279 pKa = 11.84 GGGGRR284 pKa = 11.84 LGRR287 pKa = 11.84 LLAEE291 pKa = 4.8 ARR293 pKa = 11.84 DD294 pKa = 3.98 PPCIVIRR301 pKa = 11.84 GDD303 pKa = 3.65 PNTVKK308 pKa = 10.68 CLRR311 pKa = 11.84 YY312 pKa = 9.59 RR313 pKa = 11.84 FKK315 pKa = 10.78 QQYY318 pKa = 9.82 QNLFWGVSTTWSWTWKK334 pKa = 10.29 GSEE337 pKa = 3.66 QSKK340 pKa = 9.41 RR341 pKa = 11.84 SRR343 pKa = 11.84 IIFLFTDD350 pKa = 3.48 DD351 pKa = 4.01 DD352 pKa = 3.83 QRR354 pKa = 11.84 EE355 pKa = 4.23 KK356 pKa = 11.02 FLNTVKK362 pKa = 10.53 LPKK365 pKa = 9.94 SVSYY369 pKa = 10.2 FLGSFNDD376 pKa = 3.93 YY377 pKa = 11.27
Molecular weight: 43.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.844
IPC2_protein 8.96
IPC_protein 8.916
Toseland 9.458
ProMoST 9.326
Dawson 9.765
Bjellqvist 9.502
Wikipedia 9.97
Rodwell 9.97
Grimsley 9.867
Solomon 9.794
Lehninger 9.75
Nozaki 9.516
DTASelect 9.487
Thurlkill 9.589
EMBOSS 9.897
Sillero 9.692
Patrickios 4.825
IPC_peptide 9.78
IPC2_peptide 8.112
IPC2.peptide.svr19 7.913
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2351
95
590
335.9
37.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.572 ± 0.593
2.467 ± 0.94
5.87 ± 0.395
6.423 ± 0.789
4.764 ± 0.352
5.827 ± 0.716
2.212 ± 0.267
5.359 ± 0.614
6.21 ± 0.561
9.485 ± 1.23
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.276 ± 0.231
4.679 ± 0.577
5.955 ± 0.819
3.913 ± 0.414
5.317 ± 0.616
7.571 ± 0.735
6.423 ± 0.515
5.827 ± 0.59
1.446 ± 0.347
3.403 ± 0.477
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here