Beihai hepe-like virus 10

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KJQ5|A0A1L3KJQ5_9VIRU Putative structural protein OS=Beihai hepe-like virus 10 OX=1922381 PE=4 SV=1
MM1 pKa = 7.15YY2 pKa = 10.56LILFYY7 pKa = 11.2AFFTLSSAQQLVEE20 pKa = 3.87LADD23 pKa = 3.39WQFNQIIARR32 pKa = 11.84EE33 pKa = 4.04NEE35 pKa = 3.6PNARR39 pKa = 11.84NFTCSDD45 pKa = 3.69FPNLVNWKK53 pKa = 10.14CGDD56 pKa = 3.73EE57 pKa = 4.45SPGRR61 pKa = 11.84AVARR65 pKa = 11.84VLPVGTVIPTFVPGPDD81 pKa = 3.25RR82 pKa = 11.84NSDD85 pKa = 3.26KK86 pKa = 11.13LGYY89 pKa = 8.45CTGSTTMGPYY99 pKa = 10.16NLSTIGKK106 pKa = 8.71FALEE110 pKa = 4.01LAQLEE115 pKa = 4.58VHH117 pKa = 6.51FNSHH121 pKa = 7.46FILSFDD127 pKa = 3.97IPPDD131 pKa = 3.41FDD133 pKa = 4.89FYY135 pKa = 11.74NDD137 pKa = 3.67PPYY140 pKa = 10.91LQAHH144 pKa = 5.1YY145 pKa = 9.25TGKK148 pKa = 9.66PLKK151 pKa = 9.07TQVSLKK157 pKa = 10.5QVDD160 pKa = 3.62NMLFALSQLSFEE172 pKa = 4.41QTSFDD177 pKa = 4.75DD178 pKa = 4.5KK179 pKa = 10.78EE180 pKa = 3.9QTAIDD185 pKa = 3.92VSALGMATGSVAYY198 pKa = 9.98DD199 pKa = 3.01QTTNVSSIFKK209 pKa = 8.82WFQYY213 pKa = 8.63EE214 pKa = 4.41FKK216 pKa = 11.02APNDD220 pKa = 3.7KK221 pKa = 10.15IKK223 pKa = 10.71RR224 pKa = 11.84IISPDD229 pKa = 2.51IYY231 pKa = 9.82YY232 pKa = 10.82TMFFKK237 pKa = 10.51DD238 pKa = 3.77DD239 pKa = 3.76KK240 pKa = 11.58ADD242 pKa = 3.74GGVSYY247 pKa = 9.15ITLPNPGLQLFADD260 pKa = 3.51AVEE263 pKa = 4.27RR264 pKa = 11.84TEE266 pKa = 4.49VALNYY271 pKa = 8.79QLRR274 pKa = 11.84SGITVSHH281 pKa = 6.87IDD283 pKa = 3.18IFSNIPQASYY293 pKa = 11.29NSPLTPLFSFFHH305 pKa = 6.04SDD307 pKa = 3.71KK308 pKa = 11.01YY309 pKa = 11.07ILSSWLRR316 pKa = 11.84FQNYY320 pKa = 5.74YY321 pKa = 8.73TYY323 pKa = 10.86KK324 pKa = 10.27GRR326 pKa = 11.84NITAQEE332 pKa = 3.99LNYY335 pKa = 10.78GITEE339 pKa = 4.04DD340 pKa = 3.82MRR342 pKa = 11.84NLVYY346 pKa = 9.71VQCHH350 pKa = 5.68EE351 pKa = 4.12RR352 pKa = 11.84PEE354 pKa = 4.02MCKK357 pKa = 10.58CEE359 pKa = 4.18GNYY362 pKa = 10.24SSQALAIGAFGVDD375 pKa = 3.09STYY378 pKa = 11.25TKK380 pKa = 10.8VPNTAIYY387 pKa = 9.93EE388 pKa = 4.16NQTFQVHH395 pKa = 5.44SPILHH400 pKa = 6.2HH401 pKa = 6.6VGSVPLEE408 pKa = 4.07GPYY411 pKa = 10.3TLFSSALPDD420 pKa = 3.78LPDD423 pKa = 3.38IKK425 pKa = 10.88ASNLPAWLPEE435 pKa = 4.24SVGEE439 pKa = 4.14EE440 pKa = 3.78IEE442 pKa = 4.42GVLNAVDD449 pKa = 3.92NFYY452 pKa = 11.47KK453 pKa = 10.31HH454 pKa = 6.75VKK456 pKa = 10.16DD457 pKa = 4.12FWEE460 pKa = 4.1ILQHH464 pKa = 7.25IEE466 pKa = 4.64EE467 pKa = 4.37IVEE470 pKa = 4.1VLKK473 pKa = 11.03DD474 pKa = 3.45IASLNPFGGSTPDD487 pKa = 3.27AVPVV491 pKa = 3.51

Molecular weight: 55.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KJW3|A0A1L3KJW3_9VIRU Uncharacterized protein OS=Beihai hepe-like virus 10 OX=1922381 PE=4 SV=1
MM1 pKa = 7.57SFTNFAGVPSEE12 pKa = 4.35EE13 pKa = 4.54KK14 pKa = 10.42LSEE17 pKa = 4.82LIFPTCPHH25 pKa = 6.58GEE27 pKa = 3.85ADD29 pKa = 4.08KK30 pKa = 11.51YY31 pKa = 10.7HH32 pKa = 6.78ALFNKK37 pKa = 8.55FQQINGGVSPIKK49 pKa = 10.06IDD51 pKa = 3.72AFCLGSAPKK60 pKa = 10.46QMWIAEE66 pKa = 4.16AYY68 pKa = 9.93YY69 pKa = 10.83NGLNFQGYY77 pKa = 9.33GSSKK81 pKa = 10.12SSAILEE87 pKa = 4.29VKK89 pKa = 9.64FQFMRR94 pKa = 11.84SVGQVDD100 pKa = 3.07SWANVGAAVSLDD112 pKa = 3.25SGTLKK117 pKa = 10.24TGTRR121 pKa = 11.84LVLTPFLNLRR131 pKa = 11.84VEE133 pKa = 4.52VFHH136 pKa = 6.61ATYY139 pKa = 10.06IYY141 pKa = 10.74LRR143 pKa = 11.84SNKK146 pKa = 9.86LYY148 pKa = 10.04YY149 pKa = 9.47LTAAGPSKK157 pKa = 10.46EE158 pKa = 3.92LAILFLRR165 pKa = 11.84SLLAA169 pKa = 4.53

Molecular weight: 18.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3549

146

2368

709.8

79.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.481 ± 0.587

1.662 ± 0.349

5.861 ± 0.617

7.411 ± 1.297

4.227 ± 0.825

5.889 ± 0.368

3.212 ± 0.59

5.917 ± 0.365

5.917 ± 0.876

7.608 ± 0.998

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.113 ± 0.275

4.367 ± 0.407

5.156 ± 0.426

4.142 ± 0.498

4.339 ± 0.716

6.114 ± 1.462

7.157 ± 0.773

5.466 ± 0.307

1.071 ± 0.172

3.888 ± 0.551
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski