Klugiella xanthotipulae
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2573 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A543I682|A0A543I682_9MICO NadR type nicotinamide-nucleotide adenylyltransferase OS=Klugiella xanthotipulae OX=244735 GN=FB466_0909 PE=4 SV=1
MM1 pKa = 7.15 LQVSGDD7 pKa = 3.49 TSACRR12 pKa = 11.84 PCNSGTDD19 pKa = 3.77 LAQGGLTVHH28 pKa = 6.13 NRR30 pKa = 11.84 THH32 pKa = 7.34 LAQHH36 pKa = 6.93 HH37 pKa = 5.77 GRR39 pKa = 11.84 APATADD45 pKa = 3.27 TPHH48 pKa = 7.43 RR49 pKa = 11.84 SRR51 pKa = 11.84 SGSRR55 pKa = 11.84 RR56 pKa = 11.84 LTYY59 pKa = 9.55 PALVAVLALGCGSLSLPAYY78 pKa = 8.72 ATDD81 pKa = 3.83 GDD83 pKa = 4.44 VIDD86 pKa = 4.65 GQSVAEE92 pKa = 4.38 EE93 pKa = 4.22 VQAAPPAADD102 pKa = 3.77 TAPEE106 pKa = 4.09 ALPVGEE112 pKa = 5.15 AVPEE116 pKa = 4.37 DD117 pKa = 3.84 SAPAAVEE124 pKa = 4.25 TPPVADD130 pKa = 3.84 PVAVEE135 pKa = 4.81 SPAEE139 pKa = 4.08 APVAGPPVPVAAAAAAPSGITPFAGDD165 pKa = 4.08 TILGSVSPATGDD177 pKa = 3.47 EE178 pKa = 4.29 AGGTTVILTGEE189 pKa = 4.45 CFTDD193 pKa = 3.63 AEE195 pKa = 4.25 AVIFEE200 pKa = 4.53 LTSAASFVVDD210 pKa = 4.18 SDD212 pKa = 4.02 TQITAVTPAGSGTVPVQVVGGPGCTFATLPNAFTYY247 pKa = 7.49 EE248 pKa = 4.0 APVPVVDD255 pKa = 4.4 PPVVTSLTPDD265 pKa = 3.72 RR266 pKa = 11.84 GPVGGGSDD274 pKa = 3.49 VTITGEE280 pKa = 3.9 NLADD284 pKa = 3.66 VTGVTVDD291 pKa = 4.72 GIAATGLTGVTDD303 pKa = 3.87 TSLTVTTPSHH313 pKa = 6.09 AAGPVDD319 pKa = 3.77 VVVTTTHH326 pKa = 5.48 GTSNAGLFTYY336 pKa = 10.31 HH337 pKa = 6.91 PVASVTGVSPGSGSDD352 pKa = 3.24 AGGTTVTITGTCFVGATGVTFGGTPAVSYY381 pKa = 11.08 SVLTDD386 pKa = 3.27 STLTAEE392 pKa = 4.68 TPAGTGTVDD401 pKa = 3.32 VSVATTGEE409 pKa = 4.37 CGSGATLAGSFTYY422 pKa = 9.67 TATVPAVPTITGISPTEE439 pKa = 4.26 GPDD442 pKa = 3.2 TGGTTVTVTGTNLDD456 pKa = 4.41 GIQEE460 pKa = 4.2 VTVDD464 pKa = 3.79 GTAVAQFSLLTDD476 pKa = 3.79 TSLTFVTEE484 pKa = 3.74 AHH486 pKa = 6.51 APGSVVVRR494 pKa = 11.84 VRR496 pKa = 11.84 DD497 pKa = 3.65 ASDD500 pKa = 3.4 EE501 pKa = 4.27 SATSSFTFVAAPVVEE516 pKa = 4.83 APAITSLSPTRR527 pKa = 11.84 GTIDD531 pKa = 3.19 GGTLVTITGEE541 pKa = 4.05 RR542 pKa = 11.84 LGAATAVAVGGTAVAAFTTVSDD564 pKa = 3.86 TEE566 pKa = 4.27 ITFEE570 pKa = 4.25 TAAHH574 pKa = 6.35 AAGTVDD580 pKa = 4.21 VTVTDD585 pKa = 4.4 ANGEE589 pKa = 4.19 SAPAEE594 pKa = 4.04 FTFFAPLAVTGILPATGSEE613 pKa = 4.14 AGGEE617 pKa = 4.43 TVTITGTCFTGAVDD631 pKa = 3.69 VLFGSEE637 pKa = 4.0 PATSFVVVTDD647 pKa = 3.57 TSISAVVPAGSAGLVNVTVTGSVEE671 pKa = 4.12 CGNSVLTGGYY681 pKa = 9.83 EE682 pKa = 4.2 YY683 pKa = 10.43 VTVVQPVLGSITPDD697 pKa = 2.93 SGPATGGTVVTATGSGFEE715 pKa = 4.29 TAVSVTFDD723 pKa = 3.8 GLPATNVTVVSDD735 pKa = 3.59 TEE737 pKa = 4.21 ITVVTPAHH745 pKa = 6.08 GAGVVDD751 pKa = 3.87 VVVNGPTDD759 pKa = 3.29 SALQLSPFAVAFAGITPAASNAVSFTYY786 pKa = 10.33 EE787 pKa = 3.67 AVTTPGVGEE796 pKa = 4.19 SGGAAGNVTGGAAGAPAPAPTAVGLAVTGLGGVTALLSAALLALVAGRR844 pKa = 11.84 FLLVRR849 pKa = 11.84 ARR851 pKa = 11.84 RR852 pKa = 11.84 GEE854 pKa = 3.91 LSS856 pKa = 3.05
Molecular weight: 82.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.699
IPC2_protein 3.808
IPC_protein 3.834
Toseland 3.617
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.719
Rodwell 3.656
Grimsley 3.528
Solomon 3.808
Lehninger 3.757
Nozaki 3.91
DTASelect 4.139
Thurlkill 3.656
EMBOSS 3.732
Sillero 3.948
Patrickios 1.392
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.83
Protein with the highest isoelectric point:
>tr|A0A543I6V2|A0A543I6V2_9MICO DNA-binding response OmpR family regulator OS=Klugiella xanthotipulae OX=244735 GN=FB466_1118 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 9.45 VHH17 pKa = 5.31 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 SILSARR35 pKa = 11.84 RR36 pKa = 11.84 TKK38 pKa = 10.82 GRR40 pKa = 11.84 TKK42 pKa = 10.84 LSAA45 pKa = 3.61
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2573
0
2573
880817
29
5270
342.3
36.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.104 ± 0.064
0.607 ± 0.014
5.61 ± 0.038
5.586 ± 0.052
3.104 ± 0.03
8.773 ± 0.068
2.0 ± 0.026
4.786 ± 0.038
2.098 ± 0.037
10.025 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.749 ± 0.023
2.587 ± 0.034
5.395 ± 0.04
2.9 ± 0.028
6.741 ± 0.07
6.435 ± 0.041
7.107 ± 0.091
8.82 ± 0.061
1.397 ± 0.024
2.175 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here