Lactococcus phage CHPC1183
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 40 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A650ERQ6|A0A650ERQ6_9CAUD Uncharacterized protein OS=Lactococcus phage CHPC1183 OX=2675243 GN=CHPC1183_000334 PE=4 SV=1
MM1 pKa = 7.66 AEE3 pKa = 3.91 KK4 pKa = 10.59 NIYY7 pKa = 9.19 FVNDD11 pKa = 3.28 EE12 pKa = 4.44 VEE14 pKa = 4.24 LKK16 pKa = 10.42 QVLEE20 pKa = 5.24 FIDD23 pKa = 3.55 KK24 pKa = 10.01 TDD26 pKa = 3.39 YY27 pKa = 11.08 GVNIDD32 pKa = 3.61 KK33 pKa = 10.05 TRR35 pKa = 11.84 EE36 pKa = 3.6 DD37 pKa = 3.69 VYY39 pKa = 11.64 AVVTSYY45 pKa = 11.55 SLPII49 pKa = 4.27
Molecular weight: 5.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.322
IPC2_protein 4.329
IPC_protein 4.151
Toseland 3.973
ProMoST 4.291
Dawson 4.126
Bjellqvist 4.279
Wikipedia 4.037
Rodwell 3.986
Grimsley 3.897
Solomon 4.101
Lehninger 4.062
Nozaki 4.253
DTASelect 4.406
Thurlkill 4.024
EMBOSS 4.05
Sillero 4.266
Patrickios 3.541
IPC_peptide 4.101
IPC2_peptide 4.24
IPC2.peptide.svr19 4.184
Protein with the highest isoelectric point:
>tr|A0A650ERQ7|A0A650ERQ7_9CAUD Uncharacterized protein OS=Lactococcus phage CHPC1183 OX=2675243 GN=CHPC1183_000323 PE=4 SV=1
MM1 pKa = 7.41 ATTNKK6 pKa = 8.11 VTGLEE11 pKa = 4.14 KK12 pKa = 10.14 FTEE15 pKa = 4.18 KK16 pKa = 10.32 QLKK19 pKa = 9.46 KK20 pKa = 10.55 VWLEE24 pKa = 3.65 MVDD27 pKa = 4.24 AFNSNQNTVKK37 pKa = 10.51 RR38 pKa = 11.84 SYY40 pKa = 10.61 KK41 pKa = 10.29 SSLGGDD47 pKa = 4.01 FSRR50 pKa = 11.84 YY51 pKa = 7.98 PVKK54 pKa = 10.63 FDD56 pKa = 3.19 TKK58 pKa = 10.65 KK59 pKa = 8.73 ITKK62 pKa = 9.39 QVTRR66 pKa = 11.84 SYY68 pKa = 11.08 GSQRR72 pKa = 11.84 YY73 pKa = 8.86 GRR75 pKa = 11.84 TLVKK79 pKa = 9.65 ATHH82 pKa = 6.06 EE83 pKa = 4.28 MDD85 pKa = 5.68 DD86 pKa = 3.25 KK87 pKa = 11.12 TKK89 pKa = 10.59 NIKK92 pKa = 10.07 RR93 pKa = 11.84 KK94 pKa = 9.53 LRR96 pKa = 11.84 SITNNGG102 pKa = 2.99
Molecular weight: 11.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.16
IPC2_protein 9.648
IPC_protein 9.677
Toseland 10.57
ProMoST 10.072
Dawson 10.672
Bjellqvist 10.262
Wikipedia 10.774
Rodwell 11.33
Grimsley 10.701
Solomon 10.701
Lehninger 10.687
Nozaki 10.526
DTASelect 10.262
Thurlkill 10.555
EMBOSS 10.95
Sillero 10.57
Patrickios 11.052
IPC_peptide 10.701
IPC2_peptide 8.668
IPC2.peptide.svr19 8.664
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
40
0
40
6099
40
712
152.5
17.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.198 ± 0.644
0.984 ± 0.205
6.165 ± 0.249
7.378 ± 0.576
4.624 ± 0.244
7.001 ± 0.495
1.213 ± 0.145
6.099 ± 0.477
9.149 ± 0.46
8.28 ± 0.45
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.0 ± 0.285
6.247 ± 0.377
2.64 ± 0.335
3.804 ± 0.366
3.558 ± 0.27
5.132 ± 0.44
6.673 ± 0.452
5.755 ± 0.303
1.476 ± 0.207
4.624 ± 0.448
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here