Xanthomonas phage PBR31

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G8R4V1|A0A6G8R4V1_9CAUD Uncharacterized protein OS=Xanthomonas phage PBR31 OX=2718947 GN=PBR31_00056 PE=4 SV=1
MM1 pKa = 7.53EE2 pKa = 6.64PDD4 pKa = 5.39FEE6 pKa = 6.52DD7 pKa = 5.32CDD9 pKa = 4.24VDD11 pKa = 6.03DD12 pKa = 6.5FPDD15 pKa = 4.22DD16 pKa = 3.75QPDD19 pKa = 3.3EE20 pKa = 4.25PFYY23 pKa = 11.14YY24 pKa = 10.51EE25 pKa = 5.09CDD27 pKa = 3.64DD28 pKa = 6.0DD29 pKa = 5.7EE30 pKa = 7.87DD31 pKa = 5.29IDD33 pKa = 4.55VDD35 pKa = 3.79TCLGCGRR42 pKa = 11.84ATGVHH47 pKa = 6.43PGVLAWDD54 pKa = 3.79QRR56 pKa = 11.84RR57 pKa = 11.84EE58 pKa = 4.06GCIAPQLCPRR68 pKa = 11.84CWIDD72 pKa = 5.12QGHH75 pKa = 7.2PWPSAPP81 pKa = 4.71

Molecular weight: 9.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G8R4U3|A0A6G8R4U3_9CAUD Uncharacterized protein OS=Xanthomonas phage PBR31 OX=2718947 GN=PBR31_00057 PE=4 SV=1
MM1 pKa = 7.08QFSRR5 pKa = 11.84DD6 pKa = 3.29VRR8 pKa = 11.84HH9 pKa = 5.63YY10 pKa = 10.54RR11 pKa = 11.84STPRR15 pKa = 11.84SLSSSKK21 pKa = 10.23FGPYY25 pKa = 10.02ARR27 pKa = 11.84LSVEE31 pKa = 3.78RR32 pKa = 11.84RR33 pKa = 11.84KK34 pKa = 10.57SLASKK39 pKa = 10.83AMDD42 pKa = 3.11WAYY45 pKa = 11.27VIGLGFWIGVMWYY58 pKa = 8.52LTVGLRR64 pKa = 11.84AA65 pKa = 3.78

Molecular weight: 7.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

12361

31

711

179.1

19.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.881 ± 0.439

1.213 ± 0.157

5.663 ± 0.248

5.776 ± 0.487

3.244 ± 0.237

7.872 ± 0.342

1.934 ± 0.218

5.0 ± 0.228

4.773 ± 0.273

7.952 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.896 ± 0.179

4.15 ± 0.36

5.016 ± 0.232

4.967 ± 0.376

5.639 ± 0.394

5.881 ± 0.364

6.027 ± 0.408

6.464 ± 0.286

1.861 ± 0.164

2.791 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski