Leptolyngbyaceae cyanobacterium JSC-12

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Leptolyngbyaceae; unclassified Leptolyngbyaceae

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4673 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K8GKS3|K8GKS3_9CYAN Uncharacterized protein OS=Leptolyngbyaceae cyanobacterium JSC-12 OX=864702 GN=OsccyDRAFT_2914 PE=4 SV=1
MM1 pKa = 7.55TSTSVEE7 pKa = 3.74QSAFAGLPSLLDD19 pKa = 3.46QSPLDD24 pKa = 3.6SSIPSLHH31 pKa = 6.08STNSAANLTPLQLPSFSGSSAILSVGNSIFTAVNIGALNGTVLGTGLVNSSDD83 pKa = 3.26PSDD86 pKa = 3.53YY87 pKa = 10.93FRR89 pKa = 11.84FTTSAPGSLSMSLTGMGTGDD109 pKa = 3.5ADD111 pKa = 3.28IYY113 pKa = 10.79LIQDD117 pKa = 3.36INNNGVLDD125 pKa = 4.0VNLGEE130 pKa = 4.24IIEE133 pKa = 4.35RR134 pKa = 11.84SRR136 pKa = 11.84SSSNTEE142 pKa = 3.68FLSVNLLAPGTYY154 pKa = 9.36YY155 pKa = 11.51ALVNHH160 pKa = 6.56FSGGTTNYY168 pKa = 10.34VLTLSADD175 pKa = 3.66TAGSNFLTARR185 pKa = 11.84NLGTLTASGSVSDD198 pKa = 4.43FVGNADD204 pKa = 3.64PSDD207 pKa = 3.94LYY209 pKa = 11.17RR210 pKa = 11.84FNLSNTSDD218 pKa = 3.44VTLTLSGLNNDD229 pKa = 3.52ADD231 pKa = 4.25LYY233 pKa = 11.09LIQDD237 pKa = 4.49LNQNGILDD245 pKa = 3.89NADD248 pKa = 3.42VLEE251 pKa = 3.88QSINFGSAAEE261 pKa = 4.51FIFAQGLAAGSYY273 pKa = 8.25FVQVAQYY280 pKa = 11.0SGDD283 pKa = 3.42TNYY286 pKa = 10.19TLTYY290 pKa = 10.7SVIPSDD296 pKa = 4.74AGNTLATATNLGTLAGRR313 pKa = 11.84RR314 pKa = 11.84AVNGSVSSTDD324 pKa = 3.16LVDD327 pKa = 3.83IYY329 pKa = 11.0RR330 pKa = 11.84FSTDD334 pKa = 3.21AISDD338 pKa = 3.49VRR340 pKa = 11.84LTLTGLSSDD349 pKa = 3.31ADD351 pKa = 3.72LYY353 pKa = 11.24LIQDD357 pKa = 3.87RR358 pKa = 11.84NNNGLIDD365 pKa = 4.05AGEE368 pKa = 4.23TIEE371 pKa = 4.5YY372 pKa = 10.03SILSGASSEE381 pKa = 4.75SINLSKK387 pKa = 10.8LAIGTYY393 pKa = 9.44FVAVEE398 pKa = 4.63HH399 pKa = 6.22YY400 pKa = 10.47SGSTNYY406 pKa = 9.86TLTLEE411 pKa = 4.53ADD413 pKa = 3.69SAGKK417 pKa = 10.18SLATARR423 pKa = 11.84NIGTLTSTRR432 pKa = 11.84TFNDD436 pKa = 3.7SISEE440 pKa = 3.88QDD442 pKa = 3.26SVDD445 pKa = 4.26FYY447 pKa = 11.05RR448 pKa = 11.84FNIIAPGNLTVRR460 pKa = 11.84LDD462 pKa = 3.26GMTADD467 pKa = 3.83GDD469 pKa = 4.03LYY471 pKa = 11.1LIRR474 pKa = 11.84DD475 pKa = 3.71INNNGLVEE483 pKa = 4.25INEE486 pKa = 4.4IIAVSATDD494 pKa = 5.04GISSEE499 pKa = 4.45TINFYY504 pKa = 11.29DD505 pKa = 4.23LAAGTYY511 pKa = 7.11YY512 pKa = 10.85VRR514 pKa = 11.84VNQYY518 pKa = 10.7SGNTNYY524 pKa = 10.68KK525 pKa = 10.25LSLTMEE531 pKa = 4.39PTRR534 pKa = 11.84PEE536 pKa = 4.29PGSTLTTATNLGTLSGRR553 pKa = 11.84RR554 pKa = 11.84SFSDD558 pKa = 3.4SLSLNDD564 pKa = 3.32VDD566 pKa = 5.0DD567 pKa = 5.29FYY569 pKa = 11.64RR570 pKa = 11.84FSLSTTSDD578 pKa = 3.34LQVALTGLTSNADD591 pKa = 3.37LFLIRR596 pKa = 11.84DD597 pKa = 3.68MNGNGIVDD605 pKa = 3.82SMDD608 pKa = 3.21VLQSSTSLGAVSEE621 pKa = 4.89FISLSGLAAGTYY633 pKa = 8.96FVGIRR638 pKa = 11.84GYY640 pKa = 11.8GNFTNYY646 pKa = 9.13TLSLTADD653 pKa = 3.5SAGEE657 pKa = 3.99TLATARR663 pKa = 11.84NIGVLTGSRR672 pKa = 11.84SFTDD676 pKa = 3.95FLSFQDD682 pKa = 4.34DD683 pKa = 3.28SDD685 pKa = 3.56IYY687 pKa = 10.84RR688 pKa = 11.84FSLNIMSNVSINLPGLTADD707 pKa = 3.71FDD709 pKa = 4.79LYY711 pKa = 11.06LGRR714 pKa = 11.84DD715 pKa = 3.55LNGNGLIDD723 pKa = 4.28PGEE726 pKa = 4.14TLAISATDD734 pKa = 3.82GSNSPEE740 pKa = 4.3SINISNLASGVYY752 pKa = 9.2YY753 pKa = 10.5VLVRR757 pKa = 11.84QFSGEE762 pKa = 3.83ANYY765 pKa = 9.43TLNLTATPVSS775 pKa = 3.7

Molecular weight: 81.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K8GLJ1|K8GLJ1_9CYAN Light-independent protochlorophyllide reductase subunit B OS=Leptolyngbyaceae cyanobacterium JSC-12 OX=864702 GN=chlB PE=3 SV=1
MM1 pKa = 6.61TQRR4 pKa = 11.84TLGGTNRR11 pKa = 11.84KK12 pKa = 9.1RR13 pKa = 11.84KK14 pKa = 7.65RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TKK26 pKa = 10.18NGRR29 pKa = 11.84RR30 pKa = 11.84VLKK33 pKa = 10.17ARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.47GRR40 pKa = 11.84ARR42 pKa = 11.84LAVTT46 pKa = 4.38

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4673

0

4673

1516644

23

5284

324.6

35.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.923 ± 0.038

0.989 ± 0.013

4.923 ± 0.034

5.834 ± 0.04

3.885 ± 0.025

6.832 ± 0.046

1.966 ± 0.022

6.051 ± 0.03

3.818 ± 0.033

11.153 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.961 ± 0.016

3.757 ± 0.031

5.178 ± 0.029

5.45 ± 0.036

5.716 ± 0.03

6.397 ± 0.036

5.887 ± 0.034

6.974 ± 0.028

1.456 ± 0.018

2.851 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski