Bombyx mori densovirus Zhenjiang
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B2ZA91|B2ZA91_9VIRU Nonstructural protein OS=Bombyx mori densovirus Zhenjiang OX=530042 PE=4 SV=1
MM1 pKa = 7.68 LRR3 pKa = 11.84 LTDD6 pKa = 3.46 LHH8 pKa = 7.07 KK9 pKa = 10.59 PSCEE13 pKa = 3.81 PKK15 pKa = 10.08 GLSFYY20 pKa = 10.52 AYY22 pKa = 9.68 KK23 pKa = 10.92 AFIPYY28 pKa = 9.49 RR29 pKa = 11.84 EE30 pKa = 4.2 KK31 pKa = 11.0 LSKK34 pKa = 9.85 EE35 pKa = 3.93 WNKK38 pKa = 10.6 LPVCIKK44 pKa = 10.47 DD45 pKa = 3.33 QEE47 pKa = 4.08 VDD49 pKa = 2.71 IKK51 pKa = 11.27 QEE53 pKa = 3.96 VKK55 pKa = 10.36 EE56 pKa = 4.15 DD57 pKa = 3.26 QLIRR61 pKa = 11.84 KK62 pKa = 8.42 IYY64 pKa = 9.82 KK65 pKa = 7.97 WDD67 pKa = 3.57 SVFDD71 pKa = 3.34 SWNWSKK77 pKa = 11.09 GYY79 pKa = 10.4 KK80 pKa = 9.44 LPKK83 pKa = 9.34 PLYY86 pKa = 8.65 EE87 pKa = 4.17 LLEE90 pKa = 5.14 IYY92 pKa = 9.31 MKK94 pKa = 10.49 LWKK97 pKa = 10.02 LDD99 pKa = 3.26 NRR101 pKa = 11.84 TTYY104 pKa = 10.33 PDD106 pKa = 3.06 WGEE109 pKa = 3.52 SSMIFKK115 pKa = 10.3 CYY117 pKa = 10.02 RR118 pKa = 11.84 YY119 pKa = 9.71 IRR121 pKa = 11.84 NKK123 pKa = 10.29 RR124 pKa = 11.84 MEE126 pKa = 5.19 DD127 pKa = 3.11 IEE129 pKa = 4.24 NLSTEE134 pKa = 4.36 EE135 pKa = 4.17 FNDD138 pKa = 3.75 SNFNGWPKK146 pKa = 9.85 TMWYY150 pKa = 9.25 ICRR153 pKa = 11.84 NCFDD157 pKa = 3.74 NGIVINNNSNDD168 pKa = 3.38 YY169 pKa = 11.15 YY170 pKa = 8.68 YY171 pKa = 10.78 TCDD174 pKa = 3.9 FSDD177 pKa = 3.81 NPTLLHH183 pKa = 5.17 YY184 pKa = 9.59 TDD186 pKa = 3.71 YY187 pKa = 10.99 FYY189 pKa = 11.47 YY190 pKa = 10.21 ILNNDD195 pKa = 3.66 YY196 pKa = 9.93 WCYY199 pKa = 9.85 DD200 pKa = 3.53 CKK202 pKa = 10.4 YY203 pKa = 8.8 TPLFTLTTLFCLEE216 pKa = 4.88 SINDD220 pKa = 3.74 SEE222 pKa = 4.42
Molecular weight: 27.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.96
IPC2_protein 5.207
IPC_protein 5.105
Toseland 4.991
ProMoST 5.13
Dawson 5.092
Bjellqvist 5.258
Wikipedia 4.965
Rodwell 4.991
Grimsley 4.914
Solomon 5.08
Lehninger 5.041
Nozaki 5.207
DTASelect 5.372
Thurlkill 5.003
EMBOSS 4.991
Sillero 5.27
Patrickios 1.469
IPC_peptide 5.092
IPC2_peptide 5.258
IPC2.peptide.svr19 5.186
Protein with the highest isoelectric point:
>tr|B2ZA88|B2ZA88_9VIRU Nonstructural protein OS=Bombyx mori densovirus Zhenjiang OX=530042 PE=4 SV=1
MM1 pKa = 7.46 EE2 pKa = 5.22 SKK4 pKa = 11.13 SNFRR8 pKa = 11.84 ILSNEE13 pKa = 4.18 IISKK17 pKa = 9.68 PSSLISNISSPSTSLIPRR35 pKa = 11.84 SLQKK39 pKa = 9.98 SAKK42 pKa = 9.2 RR43 pKa = 11.84 LTISSLEE50 pKa = 4.18 SGNGKK55 pKa = 7.88 QQMKK59 pKa = 10.59 SKK61 pKa = 10.17 LAQVQRR67 pKa = 11.84 ALRR70 pKa = 11.84 FCRR73 pKa = 11.84 DD74 pKa = 3.45 YY75 pKa = 11.6 NIVKK79 pKa = 9.91 YY80 pKa = 9.12 YY81 pKa = 10.43 QFMDD85 pKa = 3.96 IPPEE89 pKa = 3.9 EE90 pKa = 3.99 YY91 pKa = 10.06 RR92 pKa = 11.84 EE93 pKa = 4.09 LRR95 pKa = 11.84 THH97 pKa = 6.91 CSKK100 pKa = 11.15 DD101 pKa = 3.61 VLEE104 pKa = 4.48 TAADD108 pKa = 3.38 INYY111 pKa = 9.33 QEE113 pKa = 4.37 YY114 pKa = 10.55 YY115 pKa = 10.04 YY116 pKa = 10.98 EE117 pKa = 4.15 EE118 pKa = 4.36 VNNRR122 pKa = 11.84 LQVFLSYY129 pKa = 11.65 GDD131 pKa = 4.1 GSWGEE136 pKa = 4.08 EE137 pKa = 3.85 EE138 pKa = 4.68 EE139 pKa = 4.48 EE140 pKa = 3.62 IVMLFKK146 pKa = 11.02 HH147 pKa = 5.96 NNVDD151 pKa = 2.84 ISKK154 pKa = 9.91 FCEE157 pKa = 3.51 ALYY160 pKa = 10.81 SIFTMSDD167 pKa = 2.96 YY168 pKa = 11.07 RR169 pKa = 11.84 KK170 pKa = 10.24 NCIFLNGNIKK180 pKa = 9.09 TGKK183 pKa = 7.22 TLMMRR188 pKa = 11.84 LITGNFKK195 pKa = 10.87 CVGKK199 pKa = 10.72 GCNNGVNSEE208 pKa = 4.29 FYY210 pKa = 8.22 YY211 pKa = 10.72 QKK213 pKa = 10.81 FIGRR217 pKa = 11.84 SICVLEE223 pKa = 4.66 EE224 pKa = 4.26 PFLDD228 pKa = 4.18 PQTLEE233 pKa = 3.98 DD234 pKa = 4.2 FKK236 pKa = 11.43 SIFGGEE242 pKa = 4.25 GFDD245 pKa = 4.68 ISCKK249 pKa = 9.13 FHH251 pKa = 7.23 NPQTLNRR258 pKa = 11.84 TPFIITSNFSLLSRR272 pKa = 11.84 GHH274 pKa = 6.59 APSISEE280 pKa = 3.89 MAIKK284 pKa = 10.53 DD285 pKa = 3.24 RR286 pKa = 11.84 FYY288 pKa = 10.79 IFNFNNSYY296 pKa = 9.09 TPKK299 pKa = 10.55 CKK301 pKa = 10.33 LSPKK305 pKa = 10.21 NFVNVYY311 pKa = 9.23 NKK313 pKa = 10.75 YY314 pKa = 10.39 YY315 pKa = 10.78 GG316 pKa = 3.37
Molecular weight: 36.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.554
IPC2_protein 7.819
IPC_protein 7.658
Toseland 7.249
ProMoST 8.214
Dawson 8.375
Bjellqvist 8.697
Wikipedia 8.317
Rodwell 8.404
Grimsley 7.22
Solomon 8.463
Lehninger 8.463
Nozaki 8.858
DTASelect 8.434
Thurlkill 8.492
EMBOSS 8.58
Sillero 8.814
Patrickios 3.986
IPC_peptide 8.448
IPC2_peptide 7.614
IPC2.peptide.svr19 7.876
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3438
126
1160
573.0
65.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.363 ± 0.566
1.687 ± 0.334
6.137 ± 0.65
5.526 ± 0.455
5.061 ± 0.45
5.177 ± 0.985
1.483 ± 0.373
8.581 ± 0.629
6.457 ± 1.241
8.377 ± 0.617
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.571 ± 0.23
8.173 ± 0.643
4.101 ± 0.247
3.229 ± 0.723
4.654 ± 0.776
7.533 ± 0.916
6.457 ± 0.499
4.945 ± 0.341
1.105 ± 0.306
5.381 ± 0.591
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here