Beihai hermit crab virus 3

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Jingchuvirales; Chuviridae; Mivirus; Hermit mivirus

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KMV0|A0A1L3KMV0_9VIRU Large structural protein OS=Beihai hermit crab virus 3 OX=1922390 PE=4 SV=1
MM1 pKa = 7.46SLALLEE7 pKa = 4.62RR8 pKa = 11.84IARR11 pKa = 11.84LEE13 pKa = 4.15AEE15 pKa = 4.39VSRR18 pKa = 11.84LKK20 pKa = 11.0LQLDD24 pKa = 3.65KK25 pKa = 10.64MRR27 pKa = 11.84KK28 pKa = 7.43SEE30 pKa = 4.33RR31 pKa = 11.84EE32 pKa = 4.04STHH35 pKa = 5.36HH36 pKa = 6.18LRR38 pKa = 11.84RR39 pKa = 11.84STRR42 pKa = 11.84QVDD45 pKa = 3.56RR46 pKa = 11.84DD47 pKa = 3.81LLTLAKK53 pKa = 10.33QEE55 pKa = 4.24TLSEE59 pKa = 4.43GGSGGRR65 pKa = 11.84GQILNVYY72 pKa = 9.17EE73 pKa = 4.92DD74 pKa = 5.16LPTSHH79 pKa = 7.83PKK81 pKa = 9.25PQSSFGPGEE90 pKa = 4.19GFSPPKK96 pKa = 10.82GEE98 pKa = 4.03IQPEE102 pKa = 4.11PSCPDD107 pKa = 3.5EE108 pKa = 4.47EE109 pKa = 4.49PKK111 pKa = 10.7EE112 pKa = 4.3SPEE115 pKa = 4.06LVGVTAPPTVDD126 pKa = 2.83IAPLPEE132 pKa = 4.69PFDD135 pKa = 3.77PVGDD139 pKa = 3.9SFAALLAKK147 pKa = 10.38FNN149 pKa = 4.18

Molecular weight: 16.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KMP0|A0A1L3KMP0_9VIRU Putative glycoprotein OS=Beihai hermit crab virus 3 OX=1922390 PE=4 SV=1
MM1 pKa = 7.38SRR3 pKa = 11.84YY4 pKa = 9.14FRR6 pKa = 11.84ANHH9 pKa = 5.29QAQKK13 pKa = 10.62ASNVAVAAGATGVVGTAFGWAQHH36 pKa = 5.43QADD39 pKa = 3.95AVSLKK44 pKa = 10.81GSLSHH49 pKa = 7.7LAQDD53 pKa = 3.81YY54 pKa = 8.48MDD56 pKa = 3.88SVRR59 pKa = 11.84PEE61 pKa = 3.41TDD63 pKa = 3.39FRR65 pKa = 11.84ILQALAIHH73 pKa = 6.07MAEE76 pKa = 4.06LWVKK80 pKa = 10.35SKK82 pKa = 10.64EE83 pKa = 4.16FEE85 pKa = 4.39SPHH88 pKa = 7.84LDD90 pKa = 3.45AEE92 pKa = 4.25NLAYY96 pKa = 10.6VHH98 pKa = 6.89LAATLFCRR106 pKa = 11.84WSAPGAVSLADD117 pKa = 3.37YY118 pKa = 10.8HH119 pKa = 7.03RR120 pKa = 11.84LLSMAGGALTPAQKK134 pKa = 9.77ATIQARR140 pKa = 11.84IATAMNASHH149 pKa = 7.66DD150 pKa = 4.17GNGAWVAATAVTKK163 pKa = 10.64GKK165 pKa = 10.21YY166 pKa = 9.37RR167 pKa = 11.84DD168 pKa = 3.38VGGQGLHH175 pKa = 6.22FAANTMPHH183 pKa = 6.98RR184 pKa = 11.84IHH186 pKa = 6.95RR187 pKa = 11.84HH188 pKa = 4.26LLEE191 pKa = 4.48IAAGTAANPPAVVQANDD208 pKa = 3.33IDD210 pKa = 3.65QHH212 pKa = 7.65LIADD216 pKa = 3.64NTYY219 pKa = 10.91SVNAQLFYY227 pKa = 10.48HH228 pKa = 5.41QCVHH232 pKa = 6.89NIATAGAKK240 pKa = 7.59QRR242 pKa = 11.84KK243 pKa = 8.08MYY245 pKa = 10.72ACALMLFVSSFVRR258 pKa = 11.84TGAPTNQWVRR268 pKa = 11.84KK269 pKa = 8.26RR270 pKa = 11.84AAGLEE275 pKa = 4.07EE276 pKa = 4.37GMGLATGEE284 pKa = 4.21IEE286 pKa = 3.78IHH288 pKa = 5.68SAAFEE293 pKa = 4.29TFRR296 pKa = 11.84SLVGIKK302 pKa = 10.3DD303 pKa = 3.31VTQAQTHH310 pKa = 5.58SALSLFATHH319 pKa = 7.42ADD321 pKa = 3.5TLGVPRR327 pKa = 11.84IRR329 pKa = 11.84WMVEE333 pKa = 3.11QAAYY337 pKa = 9.86TNMTIPVTLAQVLLQYY353 pKa = 11.06DD354 pKa = 4.4YY355 pKa = 11.19IPYY358 pKa = 10.52GLLFRR363 pKa = 11.84DD364 pKa = 3.84VTQEE368 pKa = 3.46LAALVKK374 pKa = 9.95ATVAAYY380 pKa = 9.05LNPYY384 pKa = 9.49IGLHH388 pKa = 4.66SVKK391 pKa = 10.3FPSTKK396 pKa = 9.98FATIATATVEE406 pKa = 3.97ICRR409 pKa = 11.84EE410 pKa = 3.59IGMTTFDD417 pKa = 3.95RR418 pKa = 11.84YY419 pKa = 10.42KK420 pKa = 10.7GVYY423 pKa = 9.34SGCDD427 pKa = 3.1QNKK430 pKa = 9.53KK431 pKa = 10.69DD432 pKa = 4.15NIVAAFRR439 pKa = 11.84SIRR442 pKa = 11.84QDD444 pKa = 2.82SSASNLVAEE453 pKa = 4.69AMSAFMSGTVPTITVRR469 pKa = 11.84RR470 pKa = 11.84GVMNVDD476 pKa = 3.54YY477 pKa = 9.34STAAAAVALPANWDD491 pKa = 3.65ANFKK495 pKa = 10.12NLCTNHH501 pKa = 6.67RR502 pKa = 11.84VPAGGIGTFEE512 pKa = 3.33IRR514 pKa = 11.84ARR516 pKa = 11.84DD517 pKa = 3.72LYY519 pKa = 11.5AFLKK523 pKa = 8.72TAEE526 pKa = 4.21RR527 pKa = 11.84ANPNTNAAILNYY539 pKa = 8.55YY540 pKa = 8.51TVLEE544 pKa = 4.36TCGVDD549 pKa = 3.99LQTPPLDD556 pKa = 3.51NAFSVASFRR565 pKa = 11.84RR566 pKa = 11.84ADD568 pKa = 3.72PAVGTPLHH576 pKa = 6.98AYY578 pKa = 7.59MAANGDD584 pKa = 3.63RR585 pKa = 11.84AGFEE589 pKa = 4.09

Molecular weight: 63.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3805

149

2328

951.3

106.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.464 ± 2.694

1.656 ± 0.211

4.941 ± 0.245

4.993 ± 0.718

4.047 ± 0.059

6.045 ± 0.394

3.627 ± 0.558

5.861 ± 0.758

3.653 ± 0.534

11.353 ± 1.245

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.628 ± 0.273

3.89 ± 0.349

5.151 ± 0.552

4.678 ± 0.258

5.782 ± 0.458

7.227 ± 1.548

6.15 ± 0.48

6.018 ± 0.365

1.945 ± 0.369

2.891 ± 0.388
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski