Tamilnaduibacter salinus
Average proteome isoelectric point is 6.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3136 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2A2I0E8|A0A2A2I0E8_9ALTE Serine hydroxymethyltransferase OS=Tamilnaduibacter salinus OX=1484056 GN=glyA PE=3 SV=1
MM1 pKa = 7.66 PRR3 pKa = 11.84 TIPVLACITTLTLLSACGGGSQNLDD28 pKa = 3.26 TGSGGSCGAAFNPAATVRR46 pKa = 11.84 LSLGNFTISDD56 pKa = 4.35 PEE58 pKa = 4.19 PLSEE62 pKa = 4.55 TLLSDD67 pKa = 4.62 LAQGTQPNSSATPAKK82 pKa = 9.75 QDD84 pKa = 3.26 YY85 pKa = 9.48 GNQVHH90 pKa = 6.96 SDD92 pKa = 4.32 LLTQSQPFIGSTSDD106 pKa = 2.85 EE107 pKa = 5.04 DD108 pKa = 4.07 YY109 pKa = 11.18 NQARR113 pKa = 11.84 NPYY116 pKa = 10.2 DD117 pKa = 3.59 LMNKK121 pKa = 9.34 VIASGEE127 pKa = 3.92 VDD129 pKa = 3.28 NFNAGRR135 pKa = 11.84 GYY137 pKa = 10.35 ISDD140 pKa = 5.37 CIDD143 pKa = 3.38 AGNPAEE149 pKa = 4.38 YY150 pKa = 10.61 SNSANNVTIQFNEE163 pKa = 4.07 VQNEE167 pKa = 3.93 NTTEE171 pKa = 3.97 QFRR174 pKa = 11.84 YY175 pKa = 8.41 PQLRR179 pKa = 11.84 WVYY182 pKa = 10.14 TPATTNTVQRR192 pKa = 11.84 VVRR195 pKa = 11.84 YY196 pKa = 9.62 QGGDD200 pKa = 3.31 FLDD203 pKa = 4.34 GLTVGSSFASSTFSTTGYY221 pKa = 9.8 NAPQRR226 pKa = 11.84 VEE228 pKa = 3.81 ASFAADD234 pKa = 3.84 SNDD237 pKa = 3.25 EE238 pKa = 4.58 DD239 pKa = 4.14 SQQSLIIVQRR249 pKa = 11.84 FVDD252 pKa = 4.15 PDD254 pKa = 3.57 QPDD257 pKa = 3.41 DD258 pKa = 5.06 RR259 pKa = 11.84 QDD261 pKa = 2.66 EE262 pKa = 4.34 WLYY265 pKa = 11.52 SDD267 pKa = 3.5 TTGTTFDD274 pKa = 3.64 FAGATDD280 pKa = 3.69 VDD282 pKa = 4.36 CARR285 pKa = 11.84 VLVDD289 pKa = 3.87 YY290 pKa = 10.95 DD291 pKa = 3.47 MQKK294 pKa = 11.07 AYY296 pKa = 11.08 VFTSSDD302 pKa = 3.72 EE303 pKa = 4.49 PSDD306 pKa = 3.77 SPAYY310 pKa = 10.02 CGNRR314 pKa = 11.84 AMDD317 pKa = 4.36 EE318 pKa = 4.15 SDD320 pKa = 3.63 NDD322 pKa = 3.84 YY323 pKa = 8.06 PTQPVSEE330 pKa = 4.37 RR331 pKa = 11.84 KK332 pKa = 7.51 QQ333 pKa = 3.14
Molecular weight: 36.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.696
IPC2_protein 3.846
IPC_protein 3.872
Toseland 3.643
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.808
Rodwell 3.694
Grimsley 3.541
Solomon 3.859
Lehninger 3.821
Nozaki 3.973
DTASelect 4.253
Thurlkill 3.694
EMBOSS 3.821
Sillero 3.986
Patrickios 1.278
IPC_peptide 3.859
IPC2_peptide 3.961
IPC2.peptide.svr19 3.873
Protein with the highest isoelectric point:
>tr|A0A2A2I7A1|A0A2A2I7A1_9ALTE NADH dehydrogenase I subunit E OS=Tamilnaduibacter salinus OX=1484056 GN=CF392_02130 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.42 RR12 pKa = 11.84 KK13 pKa = 9.04 RR14 pKa = 11.84 AHH16 pKa = 6.12 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATANGRR28 pKa = 11.84 KK29 pKa = 9.32 VIARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.91 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3136
0
3136
1009489
31
2419
321.9
35.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.75 ± 0.047
0.89 ± 0.013
6.403 ± 0.041
6.51 ± 0.041
3.58 ± 0.03
7.927 ± 0.039
2.344 ± 0.023
4.829 ± 0.03
2.921 ± 0.036
10.504 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.5 ± 0.017
2.992 ± 0.026
4.89 ± 0.029
4.13 ± 0.028
7.408 ± 0.049
5.912 ± 0.029
5.381 ± 0.03
7.245 ± 0.041
1.409 ± 0.02
2.474 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here