Amino acid dipepetide frequency for bacterium HR15

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.885AlaAla: 7.885 ± 0.113	1.13AlaCys: 1.13 ± 0.039	4.236AlaAsp: 4.236 ± 0.08	6.137AlaGlu: 6.137 ± 0.125	3.853AlaPhe: 3.853 ± 0.074	6.818AlaGly: 6.818 ± 0.098	2.335AlaHis: 2.335 ± 0.061	5.628AlaIle: 5.628 ± 0.079	2.36AlaLys: 2.36 ± 0.055	11.909AlaLeu: 11.909 ± 0.165	2.207AlaMet: 2.207 ± 0.055	2.557AlaAsn: 2.557 ± 0.06	4.731AlaPro: 4.731 ± 0.079	5.023AlaGln: 5.023 ± 0.088	7.58AlaArg: 7.58 ± 0.109	4.509AlaSer: 4.509 ± 0.076	4.859AlaThr: 4.859 ± 0.087	6.618AlaVal: 6.618 ± 0.088	1.521AlaTrp: 1.521 ± 0.046	2.911AlaTyr: 2.911 ± 0.057	0.0AlaXaa: 0.0 ± 0.0
Cys	0.967CysAla: 0.967 ± 0.032	0.162CysCys: 0.162 ± 0.016	0.48CysAsp: 0.48 ± 0.026	0.661CysGlu: 0.661 ± 0.027	0.385CysPhe: 0.385 ± 0.024	0.892CysGly: 0.892 ± 0.037	0.306CysHis: 0.306 ± 0.023	0.529CysIle: 0.529 ± 0.029	0.224CysLys: 0.224 ± 0.016	1.124CysLeu: 1.124 ± 0.037	0.272CysMet: 0.272 ± 0.017	0.257CysAsn: 0.257 ± 0.016	0.795CysPro: 0.795 ± 0.034	0.411CysGln: 0.411 ± 0.024	0.736CysArg: 0.736 ± 0.03	0.497CysSer: 0.497 ± 0.026	0.576CysThr: 0.576 ± 0.061	0.749CysVal: 0.749 ± 0.03	0.231CysTrp: 0.231 ± 0.017	0.345CysTyr: 0.345 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	4.931AspAla: 4.931 ± 0.083	0.412AspCys: 0.412 ± 0.025	2.053AspAsp: 2.053 ± 0.052	3.255AspGlu: 3.255 ± 0.066	1.71AspPhe: 1.71 ± 0.047	3.996AspGly: 3.996 ± 0.11	0.857AspHis: 0.857 ± 0.031	2.33AspIle: 2.33 ± 0.058	1.104AspLys: 1.104 ± 0.044	4.913AspLeu: 4.913 ± 0.089	0.915AspMet: 0.915 ± 0.032	1.083AspAsn: 1.083 ± 0.04	3.504AspPro: 3.504 ± 0.074	1.353AspGln: 1.353 ± 0.038	2.783AspArg: 2.783 ± 0.058	2.044AspSer: 2.044 ± 0.048	2.438AspThr: 2.438 ± 0.059	2.78AspVal: 2.78 ± 0.057	1.092AspTrp: 1.092 ± 0.041	1.766AspTyr: 1.766 ± 0.043	0.0AspXaa: 0.0 ± 0.0
Glu	6.793GluAla: 6.793 ± 0.117	0.597GluCys: 0.597 ± 0.028	2.202GluAsp: 2.202 ± 0.059	4.362GluGlu: 4.362 ± 0.102	1.949GluPhe: 1.949 ± 0.047	4.794GluGly: 4.794 ± 0.083	1.53GluHis: 1.53 ± 0.042	3.543GluIle: 3.543 ± 0.071	2.02GluLys: 2.02 ± 0.061	6.434GluLeu: 6.434 ± 0.095	1.664GluMet: 1.664 ± 0.043	1.53GluAsn: 1.53 ± 0.04	3.812GluPro: 3.812 ± 0.066	4.175GluGln: 4.175 ± 0.104	6.462GluArg: 6.462 ± 0.117	2.828GluSer: 2.828 ± 0.059	3.206GluThr: 3.206 ± 0.063	4.963GluVal: 4.963 ± 0.084	1.39GluTrp: 1.39 ± 0.046	1.792GluTyr: 1.792 ± 0.049	0.0GluXaa: 0.0 ± 0.0
Phe	3.443PheAla: 3.443 ± 0.062	0.443PheCys: 0.443 ± 0.025	1.76PheAsp: 1.76 ± 0.043	2.42PheGlu: 2.42 ± 0.06	1.272PhePhe: 1.272 ± 0.045	3.243PheGly: 3.243 ± 0.068	0.793PheHis: 0.793 ± 0.034	1.657PheIle: 1.657 ± 0.048	0.827PheLys: 0.827 ± 0.028	3.409PheLeu: 3.409 ± 0.068	0.603PheMet: 0.603 ± 0.027	1.097PheAsn: 1.097 ± 0.041	1.842PhePro: 1.842 ± 0.044	1.296PheGln: 1.296 ± 0.043	2.592PheArg: 2.592 ± 0.051	1.926PheSer: 1.926 ± 0.059	2.121PheThr: 2.121 ± 0.053	2.523PheVal: 2.523 ± 0.053	0.748PheTrp: 0.748 ± 0.03	1.394PheTyr: 1.394 ± 0.038	0.0PheXaa: 0.0 ± 0.0
Gly	6.853GlyAla: 6.853 ± 0.108	1.125GlyCys: 1.125 ± 0.045	3.778GlyAsp: 3.778 ± 0.071	5.07GlyGlu: 5.07 ± 0.082	3.034GlyPhe: 3.034 ± 0.063	6.332GlyGly: 6.332 ± 0.137	1.534GlyHis: 1.534 ± 0.047	4.517GlyIle: 4.517 ± 0.08	2.962GlyLys: 2.962 ± 0.057	7.375GlyLeu: 7.375 ± 0.1	1.962GlyMet: 1.962 ± 0.046	2.629GlyAsn: 2.629 ± 0.08	1.761GlyPro: 1.761 ± 0.039	2.947GlyGln: 2.947 ± 0.062	5.275GlyArg: 5.275 ± 0.082	4.334GlySer: 4.334 ± 0.099	4.574GlyThr: 4.574 ± 0.093	6.288GlyVal: 6.288 ± 0.09	1.803GlyTrp: 1.803 ± 0.048	3.004GlyTyr: 3.004 ± 0.061	0.0GlyXaa: 0.0 ± 0.0
His	2.443HisAla: 2.443 ± 0.052	0.26HisCys: 0.26 ± 0.017	0.941HisAsp: 0.941 ± 0.032	1.406HisGlu: 1.406 ± 0.049	0.888HisPhe: 0.888 ± 0.03	1.619HisGly: 1.619 ± 0.044	0.694HisHis: 0.694 ± 0.028	1.144HisIle: 1.144 ± 0.038	0.51HisLys: 0.51 ± 0.025	2.81HisLeu: 2.81 ± 0.067	0.35HisMet: 0.35 ± 0.017	0.531HisAsn: 0.531 ± 0.025	1.857HisPro: 1.857 ± 0.046	0.989HisGln: 0.989 ± 0.037	1.72HisArg: 1.72 ± 0.048	1.036HisSer: 1.036 ± 0.038	1.249HisThr: 1.249 ± 0.043	1.181HisVal: 1.181 ± 0.037	0.467HisTrp: 0.467 ± 0.022	0.828HisTyr: 0.828 ± 0.034	0.0HisXaa: 0.0 ± 0.0
Ile	5.934IleAla: 5.934 ± 0.09	0.495IleCys: 0.495 ± 0.021	2.902IleAsp: 2.902 ± 0.053	4.218IleGlu: 4.218 ± 0.074	1.43IlePhe: 1.43 ± 0.049	4.654IleGly: 4.654 ± 0.078	1.044IleHis: 1.044 ± 0.034	2.277IleIle: 2.277 ± 0.055	1.285IleLys: 1.285 ± 0.048	5.061IleLeu: 5.061 ± 0.082	0.683IleMet: 0.683 ± 0.03	1.352IleAsn: 1.352 ± 0.036	3.084IlePro: 3.084 ± 0.063	2.012IleGln: 2.012 ± 0.048	4.065IleArg: 4.065 ± 0.073	2.242IleSer: 2.242 ± 0.053	2.914IleThr: 2.914 ± 0.058	4.062IleVal: 4.062 ± 0.069	0.748IleTrp: 0.748 ± 0.031	1.552IleTyr: 1.552 ± 0.044	0.0IleXaa: 0.0 ± 0.0
Lys	2.678LysAla: 2.678 ± 0.067	0.206LysCys: 0.206 ± 0.015	0.933LysAsp: 0.933 ± 0.038	1.844LysGlu: 1.844 ± 0.057	0.674LysPhe: 0.674 ± 0.027	2.08LysGly: 2.08 ± 0.049	0.599LysHis: 0.599 ± 0.028	1.34LysIle: 1.34 ± 0.043	0.901LysLys: 0.901 ± 0.044	2.541LysLeu: 2.541 ± 0.065	0.654LysMet: 0.654 ± 0.03	0.655LysAsn: 0.655 ± 0.026	2.072LysPro: 2.072 ± 0.059	1.47LysGln: 1.47 ± 0.046	2.527LysArg: 2.527 ± 0.062	1.198LysSer: 1.198 ± 0.034	1.374LysThr: 1.374 ± 0.04	2.158LysVal: 2.158 ± 0.053	0.444LysTrp: 0.444 ± 0.022	0.688LysTyr: 0.688 ± 0.031	0.0LysXaa: 0.0 ± 0.0
Leu	10.002LeuAla: 10.002 ± 0.151	1.277LeuCys: 1.277 ± 0.036	4.489LeuAsp: 4.489 ± 0.076	6.644LeuGlu: 6.644 ± 0.104	4.085LeuPhe: 4.085 ± 0.083	7.715LeuGly: 7.715 ± 0.11	2.697LeuHis: 2.697 ± 0.063	6.004LeuIle: 6.004 ± 0.087	3.404LeuLys: 3.404 ± 0.076	12.973LeuLeu: 12.973 ± 0.169	2.878LeuMet: 2.878 ± 0.061	3.348LeuAsn: 3.348 ± 0.073	6.907LeuPro: 6.907 ± 0.102	5.87LeuGln: 5.87 ± 0.099	8.856LeuArg: 8.856 ± 0.118	5.905LeuSer: 5.905 ± 0.091	6.279LeuThr: 6.279 ± 0.095	7.231LeuVal: 7.231 ± 0.107	2.088LeuTrp: 2.088 ± 0.062	3.419LeuTyr: 3.419 ± 0.067	0.0LeuXaa: 0.0 ± 0.0
Met	2.04MetAla: 2.04 ± 0.052	0.182MetCys: 0.182 ± 0.016	0.83MetAsp: 0.83 ± 0.03	1.55MetGlu: 1.55 ± 0.044	0.581MetPhe: 0.581 ± 0.026	1.904MetGly: 1.904 ± 0.051	0.487MetHis: 0.487 ± 0.022	0.889MetIle: 0.889 ± 0.034	0.664MetLys: 0.664 ± 0.027	2.597MetLeu: 2.597 ± 0.058	0.591MetMet: 0.591 ± 0.026	0.63MetAsn: 0.63 ± 0.025	1.516MetPro: 1.516 ± 0.048	1.015MetGln: 1.015 ± 0.036	2.304MetArg: 2.304 ± 0.051	1.104MetSer: 1.104 ± 0.033	1.019MetThr: 1.019 ± 0.032	1.638MetVal: 1.638 ± 0.043	0.275MetTrp: 0.275 ± 0.02	0.414MetTyr: 0.414 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	3.025AsnAla: 3.025 ± 0.053	0.321AsnCys: 0.321 ± 0.025	1.192AsnAsp: 1.192 ± 0.039	1.468AsnGlu: 1.468 ± 0.039	0.98AsnPhe: 0.98 ± 0.04	2.71AsnGly: 2.71 ± 0.076	0.525AsnHis: 0.525 ± 0.025	1.267AsnIle: 1.267 ± 0.042	0.637AsnLys: 0.637 ± 0.029	3.328AsnLeu: 3.328 ± 0.061	0.457AsnMet: 0.457 ± 0.021	0.937AsnAsn: 0.937 ± 0.05	2.541AsnPro: 2.541 ± 0.06	1.024AsnGln: 1.024 ± 0.035	2.191AsnArg: 2.191 ± 0.045	1.222AsnSer: 1.222 ± 0.042	1.578AsnThr: 1.578 ± 0.052	1.421AsnVal: 1.421 ± 0.041	0.544AsnTrp: 0.544 ± 0.031	0.877AsnTyr: 0.877 ± 0.034	0.0AsnXaa: 0.0 ± 0.0
Pro	5.266ProAla: 5.266 ± 0.091	0.558ProCys: 0.558 ± 0.031	3.941ProAsp: 3.941 ± 0.077	4.192ProGlu: 4.192 ± 0.08	2.29ProPhe: 2.29 ± 0.054	3.635ProGly: 3.635 ± 0.066	1.474ProHis: 1.474 ± 0.038	2.958ProIle: 2.958 ± 0.059	1.473ProLys: 1.473 ± 0.041	6.011ProLeu: 6.011 ± 0.095	1.351ProMet: 1.351 ± 0.034	2.128ProAsn: 2.128 ± 0.054	4.187ProPro: 4.187 ± 0.072	2.069ProGln: 2.069 ± 0.049	3.521ProArg: 3.521 ± 0.068	3.247ProSer: 3.247 ± 0.06	3.291ProThr: 3.291 ± 0.045	4.062ProVal: 4.062 ± 0.068	0.625ProTrp: 0.625 ± 0.03	1.905ProTyr: 1.905 ± 0.049	0.0ProXaa: 0.0 ± 0.0
Gln	4.75GlnAla: 4.75 ± 0.095	0.305GlnCys: 0.305 ± 0.02	1.313GlnAsp: 1.313 ± 0.037	2.855GlnGlu: 2.855 ± 0.065	1.399GlnPhe: 1.399 ± 0.044	3.261GlnGly: 3.261 ± 0.06	1.07GlnHis: 1.07 ± 0.042	2.671GlnIle: 2.671 ± 0.059	1.253GlnLys: 1.253 ± 0.045	4.968GlnLeu: 4.968 ± 0.107	1.215GlnMet: 1.215 ± 0.033	1.071GlnAsn: 1.071 ± 0.034	2.906GlnPro: 2.906 ± 0.055	3.117GlnGln: 3.117 ± 0.091	4.024GlnArg: 4.024 ± 0.091	2.048GlnSer: 2.048 ± 0.048	2.362GlnThr: 2.362 ± 0.048	3.823GlnVal: 3.823 ± 0.072	0.762GlnTrp: 0.762 ± 0.031	1.079GlnTyr: 1.079 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	7.531ArgAla: 7.531 ± 0.108	0.802ArgCys: 0.802 ± 0.033	3.163ArgAsp: 3.163 ± 0.061	5.81ArgGlu: 5.81 ± 0.085	3.133ArgPhe: 3.133 ± 0.061	4.875ArgGly: 4.875 ± 0.077	1.864ArgHis: 1.864 ± 0.053	4.24ArgIle: 4.24 ± 0.063	2.197ArgLys: 2.197 ± 0.058	9.339ArgLeu: 9.339 ± 0.119	1.94ArgMet: 1.94 ± 0.047	1.972ArgAsn: 1.972 ± 0.05	3.413ArgPro: 3.413 ± 0.066	3.703ArgGln: 3.703 ± 0.074	5.807ArgArg: 5.807 ± 0.098	3.218ArgSer: 3.218 ± 0.057	4.042ArgThr: 4.042 ± 0.072	6.133ArgVal: 6.133 ± 0.097	2.133ArgTrp: 2.133 ± 0.059	2.953ArgTyr: 2.953 ± 0.061	0.0ArgXaa: 0.0 ± 0.0
Ser	4.494SerAla: 4.494 ± 0.08	0.469SerCys: 0.469 ± 0.022	2.645SerAsp: 2.645 ± 0.066	3.265SerGlu: 3.265 ± 0.068	1.669SerPhe: 1.669 ± 0.049	4.477SerGly: 4.477 ± 0.097	1.04SerHis: 1.04 ± 0.041	2.256SerIle: 2.256 ± 0.049	1.137SerLys: 1.137 ± 0.037	5.484SerLeu: 5.484 ± 0.088	0.966SerMet: 0.966 ± 0.034	1.393SerAsn: 1.393 ± 0.052	3.268SerPro: 3.268 ± 0.072	1.778SerGln: 1.778 ± 0.042	3.24SerArg: 3.24 ± 0.06	2.538SerSer: 2.538 ± 0.064	2.515SerThr: 2.515 ± 0.065	3.462SerVal: 3.462 ± 0.076	0.896SerTrp: 0.896 ± 0.036	1.354SerTyr: 1.354 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	4.566ThrAla: 4.566 ± 0.074	0.485ThrCys: 0.485 ± 0.027	2.45ThrAsp: 2.45 ± 0.055	2.915ThrGlu: 2.915 ± 0.074	1.774ThrPhe: 1.774 ± 0.052	4.835ThrGly: 4.835 ± 0.085	1.188ThrHis: 1.188 ± 0.033	2.748ThrIle: 2.748 ± 0.068	0.952ThrLys: 0.952 ± 0.037	7.085ThrLeu: 7.085 ± 0.097	0.974ThrMet: 0.974 ± 0.031	1.399ThrAsn: 1.399 ± 0.044	3.963ThrPro: 3.963 ± 0.067	1.872ThrGln: 1.872 ± 0.049	3.775ThrArg: 3.775 ± 0.062	2.204ThrSer: 2.204 ± 0.052	2.766ThrThr: 2.766 ± 0.075	4.003ThrVal: 4.003 ± 0.082	0.687ThrTrp: 0.687 ± 0.03	1.538ThrTyr: 1.538 ± 0.047	0.0ThrXaa: 0.0 ± 0.0
Val	6.797ValAla: 6.797 ± 0.109	0.795ValCys: 0.795 ± 0.03	3.382ValAsp: 3.382 ± 0.071	4.79ValGlu: 4.79 ± 0.082	2.483ValPhe: 2.483 ± 0.056	5.488ValGly: 5.488 ± 0.088	1.598ValHis: 1.598 ± 0.05	3.737ValIle: 3.737 ± 0.066	1.956ValLys: 1.956 ± 0.057	8.163ValLeu: 8.163 ± 0.1	1.574ValMet: 1.574 ± 0.041	2.113ValAsn: 2.113 ± 0.053	3.987ValPro: 3.987 ± 0.068	3.575ValGln: 3.575 ± 0.065	6.147ValArg: 6.147 ± 0.092	3.835ValSer: 3.835 ± 0.085	2.393ValThr: 2.393 ± 0.067	5.924ValVal: 5.924 ± 0.103	1.261ValTrp: 1.261 ± 0.041	2.298ValTyr: 2.298 ± 0.062	0.0ValXaa: 0.0 ± 0.0
Trp	1.395TrpAla: 1.395 ± 0.038	0.215TrpCys: 0.215 ± 0.015	0.807TrpAsp: 0.807 ± 0.035	1.047TrpGlu: 1.047 ± 0.037	0.654TrpPhe: 0.654 ± 0.031	1.311TrpGly: 1.311 ± 0.038	0.513TrpHis: 0.513 ± 0.025	0.896TrpIle: 0.896 ± 0.036	0.458TrpLys: 0.458 ± 0.023	2.862TrpLeu: 2.862 ± 0.076	0.499TrpMet: 0.499 ± 0.021	0.59TrpAsn: 0.59 ± 0.029	0.224TrpPro: 0.224 ± 0.017	1.209TrpGln: 1.209 ± 0.039	1.799TrpArg: 1.799 ± 0.052	1.05TrpSer: 1.05 ± 0.042	0.901TrpThr: 0.901 ± 0.039	1.381TrpVal: 1.381 ± 0.041	0.451TrpTrp: 0.451 ± 0.024	0.533TrpTyr: 0.533 ± 0.03	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.013TyrAla: 3.013 ± 0.062	0.361TyrCys: 0.361 ± 0.021	1.823TyrAsp: 1.823 ± 0.055	2.137TyrGlu: 2.137 ± 0.048	1.165TyrPhe: 1.165 ± 0.039	2.518TyrGly: 2.518 ± 0.071	0.775TyrHis: 0.775 ± 0.03	1.382TyrIle: 1.382 ± 0.04	0.714TyrLys: 0.714 ± 0.031	3.686TyrLeu: 3.686 ± 0.07	0.474TyrMet: 0.474 ± 0.02	0.972TyrAsn: 0.972 ± 0.033	1.814TyrPro: 1.814 ± 0.051	1.345TyrGln: 1.345 ± 0.037	2.863TyrArg: 2.863 ± 0.063	1.361TyrSer: 1.361 ± 0.047	1.725TyrThr: 1.725 ± 0.044	1.892TyrVal: 1.892 ± 0.05	0.628TyrTrp: 0.628 ± 0.029	1.173TyrTyr: 1.173 ± 0.044	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2671 proteins (916987 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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