Alcaligenaceae bacterium

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Alcaligenaceae; unclassified Alcaligenaceae

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5394 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q4ANJ2|A0A4Q4ANJ2_9BURK Extracellular solute-binding protein (Fragment) OS=Alcaligenaceae bacterium OX=2021368 GN=EON54_12825 PE=4 SV=1
MM1 pKa = 7.57HH2 pKa = 7.12SLARR6 pKa = 11.84KK7 pKa = 9.16KK8 pKa = 10.93SSITEE13 pKa = 4.05LTSEE17 pKa = 4.5EE18 pKa = 3.45ISYY21 pKa = 10.77ISGGDD26 pKa = 3.49DD27 pKa = 3.41GGACGDD33 pKa = 4.33SGSCGAGDD41 pKa = 3.85SGSCGGDD48 pKa = 2.72GGACDD53 pKa = 3.94TGATASDD60 pKa = 3.72TSAPPSGGLTGSVIGAIGAAIGLAGAATPVGAVAAAMAALGAAISAMSNPAMGGAGTDD118 pKa = 2.94SGSNVTGNAMGDD130 pKa = 3.77LPGGPP135 pKa = 4.11

Molecular weight: 12.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q4AZ68|A0A4Q4AZ68_9BURK Phage tail protein OS=Alcaligenaceae bacterium OX=2021368 GN=EON54_07410 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.43RR14 pKa = 11.84THH16 pKa = 5.96GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 9.69SRR25 pKa = 11.84SGRR28 pKa = 11.84AVLSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5394

0

5394

1188387

19

1867

220.3

24.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.198 ± 0.04

0.994 ± 0.012

5.405 ± 0.03

5.468 ± 0.033

3.708 ± 0.024

7.611 ± 0.035

2.235 ± 0.019

4.879 ± 0.028

3.913 ± 0.032

10.133 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.418 ± 0.017

3.09 ± 0.024

4.886 ± 0.024

3.861 ± 0.023

6.801 ± 0.033

6.418 ± 0.026

5.496 ± 0.034

7.479 ± 0.032

1.471 ± 0.018

2.537 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski