Hibiscus golden mosaic virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6FVQ7|A0A4D6FVQ7_9GEMI Movement protein BC1 OS=Hibiscus golden mosaic virus OX=2569569 GN=MP PE=3 SV=1
MM1 pKa = 7.27PRR3 pKa = 11.84KK4 pKa = 10.15GSFNLYY10 pKa = 10.26AKK12 pKa = 10.48NFFLTYY18 pKa = 9.02PKK20 pKa = 10.59CSLTKK25 pKa = 10.27EE26 pKa = 4.2QVLSQLLGLEE36 pKa = 4.04TAVNKK41 pKa = 10.4KK42 pKa = 9.9FIKK45 pKa = 9.69ICRR48 pKa = 11.84EE49 pKa = 3.52LHH51 pKa = 6.25EE52 pKa = 4.95NGEE55 pKa = 4.17PHH57 pKa = 6.34IHH59 pKa = 6.38VLLQFEE65 pKa = 5.26GKK67 pKa = 9.84LQTTNKK73 pKa = 9.98RR74 pKa = 11.84FFDD77 pKa = 3.96LVSPSSSAQFHH88 pKa = 6.38GNYY91 pKa = 9.59QGAKK95 pKa = 9.25SSSDD99 pKa = 3.1VKK101 pKa = 11.22SYY103 pKa = 11.05VDD105 pKa = 3.3KK106 pKa = 11.56DD107 pKa = 3.52GDD109 pKa = 3.74VLEE112 pKa = 4.6WGEE115 pKa = 3.86FQIDD119 pKa = 3.32SRR121 pKa = 11.84SARR124 pKa = 11.84GGQQTVNEE132 pKa = 4.74SYY134 pKa = 11.49AKK136 pKa = 10.22ALNSGSVTEE145 pKa = 4.22ALRR148 pKa = 11.84VLKK151 pKa = 10.46EE152 pKa = 3.95EE153 pKa = 3.87QPRR156 pKa = 11.84DD157 pKa = 3.62YY158 pKa = 10.84FSNFHH163 pKa = 6.46NLLANAEE170 pKa = 4.26RR171 pKa = 11.84IFAKK175 pKa = 10.54VPEE178 pKa = 4.14PWVPRR183 pKa = 11.84FHH185 pKa = 6.94LSSFTEE191 pKa = 4.42VPVEE195 pKa = 3.82MQEE198 pKa = 4.06WVDD201 pKa = 3.65SYY203 pKa = 11.29FGRR206 pKa = 11.84GAAARR211 pKa = 11.84PEE213 pKa = 4.15RR214 pKa = 11.84PLSIIIEE221 pKa = 4.46GSSRR225 pKa = 11.84TGKK228 pKa = 8.57TMWARR233 pKa = 11.84ALGVHH238 pKa = 6.56NYY240 pKa = 10.5LSGHH244 pKa = 6.87LDD246 pKa = 3.28FNSRR250 pKa = 11.84VFSNDD255 pKa = 2.3VEE257 pKa = 4.65YY258 pKa = 11.41NVIDD262 pKa = 4.32DD263 pKa = 4.14VAPHH267 pKa = 5.29YY268 pKa = 10.97LKK270 pKa = 10.78LKK272 pKa = 9.12HH273 pKa = 5.7WKK275 pKa = 9.57EE276 pKa = 4.04LIGAQRR282 pKa = 11.84DD283 pKa = 3.53WQSNCKK289 pKa = 9.41YY290 pKa = 10.21GKK292 pKa = 9.18PVQIKK297 pKa = 10.19GGIPSIVLCNPGEE310 pKa = 4.16GASYY314 pKa = 11.22KK315 pKa = 10.86DD316 pKa = 3.87FLEE319 pKa = 4.74KK320 pKa = 10.07PDD322 pKa = 3.85NASLKK327 pKa = 10.57SWTLHH332 pKa = 4.43NAKK335 pKa = 10.29FIFLDD340 pKa = 3.5SPLYY344 pKa = 9.43QTAQEE349 pKa = 4.22EE350 pKa = 5.22GSS352 pKa = 3.51

Molecular weight: 39.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6FYE0|A0A4D6FYE0_9GEMI AC4 protein OS=Hibiscus golden mosaic virus OX=2569569 GN=AC4 PE=3 SV=1
MM1 pKa = 7.98PKK3 pKa = 9.65RR4 pKa = 11.84DD5 pKa = 3.82APWRR9 pKa = 11.84LMAGTSKK16 pKa = 10.62VSRR19 pKa = 11.84SSNSPRR25 pKa = 11.84VGPKK29 pKa = 9.75VNKK32 pKa = 7.87ATEE35 pKa = 4.11WVHH38 pKa = 7.42RR39 pKa = 11.84PMYY42 pKa = 9.6RR43 pKa = 11.84KK44 pKa = 9.19PRR46 pKa = 11.84IYY48 pKa = 10.07RR49 pKa = 11.84AYY51 pKa = 8.6RR52 pKa = 11.84TPDD55 pKa = 3.07VPRR58 pKa = 11.84GCEE61 pKa = 4.34GPCKK65 pKa = 9.32VQSYY69 pKa = 6.94EE70 pKa = 3.74QRR72 pKa = 11.84HH73 pKa = 6.34DD74 pKa = 3.14ISHH77 pKa = 6.63TGKK80 pKa = 10.46VMCISDD86 pKa = 3.56VTRR89 pKa = 11.84GNGITHH95 pKa = 6.78RR96 pKa = 11.84VGKK99 pKa = 9.44RR100 pKa = 11.84FCVKK104 pKa = 9.92SVYY107 pKa = 10.16ILGKK111 pKa = 9.37IWMDD115 pKa = 3.39EE116 pKa = 4.0NIKK119 pKa = 10.61LKK121 pKa = 10.73NHH123 pKa = 5.95TNSVMFWLVRR133 pKa = 11.84DD134 pKa = 3.7RR135 pKa = 11.84RR136 pKa = 11.84PYY138 pKa = 8.05GTPMDD143 pKa = 4.6FGQVFNMFDD152 pKa = 3.99NEE154 pKa = 4.11PSTATVKK161 pKa = 10.85NDD163 pKa = 2.82LRR165 pKa = 11.84DD166 pKa = 3.54RR167 pKa = 11.84FQVMHH172 pKa = 6.9KK173 pKa = 10.21FYY175 pKa = 11.22GKK177 pKa = 8.61VTGGQYY183 pKa = 11.07ASNEE187 pKa = 3.69QAIVKK192 pKa = 9.05RR193 pKa = 11.84FWKK196 pKa = 10.22VNNHH200 pKa = 4.48VVYY203 pKa = 10.67NHH205 pKa = 5.8QEE207 pKa = 3.47AGKK210 pKa = 10.41YY211 pKa = 8.39EE212 pKa = 4.09NHH214 pKa = 6.52TEE216 pKa = 3.99NALLLYY222 pKa = 7.29MACTHH227 pKa = 7.07ASNPVYY233 pKa = 9.86ATLKK237 pKa = 9.47IRR239 pKa = 11.84IYY241 pKa = 10.59FYY243 pKa = 11.38DD244 pKa = 4.2SITNN248 pKa = 3.6

Molecular weight: 28.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1509

97

352

215.6

24.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.036 ± 0.627

1.789 ± 0.226

4.97 ± 0.413

4.904 ± 0.634

4.44 ± 0.385

5.434 ± 0.551

3.91 ± 0.324

5.302 ± 0.6

5.964 ± 0.572

7.157 ± 0.691

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.187 ± 0.501

5.302 ± 0.498

4.97 ± 0.584

3.645 ± 0.594

7.687 ± 0.878

9.742 ± 1.166

5.103 ± 0.599

6.627 ± 0.531

1.59 ± 0.144

4.241 ± 0.694
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski