Olsenella uli (strain ATCC 49627 / DSM 7084 / CIP 109912 / JCM 12494 / NCIMB 702895 / VPI D76D-27C) (Lactobacillus uli)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Coriobacteriales; Atopobiaceae; Olsenella; Olsenella uli

Average proteome isoelectric point is 5.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1739 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E1QYB0|E1QYB0_OLSUV ABC transporter related protein OS=Olsenella uli (strain ATCC 49627 / DSM 7084 / CIP 109912 / JCM 12494 / NCIMB 702895 / VPI D76D-27C) OX=633147 GN=Olsu_0248 PE=4 SV=1
MM1 pKa = 7.17TRR3 pKa = 11.84RR4 pKa = 11.84GLLAASLALAGGALGLAGCGLLRR27 pKa = 11.84GRR29 pKa = 11.84EE30 pKa = 4.05EE31 pKa = 4.13EE32 pKa = 4.36VPDD35 pKa = 3.55VTISSGEE42 pKa = 3.96DD43 pKa = 2.86ALAYY47 pKa = 10.06ALQALGEE54 pKa = 4.24RR55 pKa = 11.84YY56 pKa = 10.31GEE58 pKa = 4.26GFSQAEE64 pKa = 4.23GTEE67 pKa = 4.13ITSYY71 pKa = 10.58MPDD74 pKa = 3.69DD75 pKa = 3.66TDD77 pKa = 4.11LTYY80 pKa = 10.62EE81 pKa = 4.07LCARR85 pKa = 11.84PDD87 pKa = 3.57SDD89 pKa = 3.43PGKK92 pKa = 10.95VFGCDD97 pKa = 2.75VSTDD101 pKa = 3.58RR102 pKa = 11.84EE103 pKa = 4.35TGRR106 pKa = 11.84LLDD109 pKa = 3.95VLEE112 pKa = 5.29DD113 pKa = 5.36DD114 pKa = 3.74YY115 pKa = 11.75TQYY118 pKa = 11.49LFKK121 pKa = 10.99DD122 pKa = 3.48QMEE125 pKa = 4.85GPFLEE130 pKa = 4.67VVRR133 pKa = 11.84GLSGLAGYY141 pKa = 8.66SVQMAEE147 pKa = 4.68PYY149 pKa = 10.76VGDD152 pKa = 3.82RR153 pKa = 11.84DD154 pKa = 3.68WRR156 pKa = 11.84PDD158 pKa = 3.44EE159 pKa = 4.52LDD161 pKa = 3.72AYY163 pKa = 9.9VNNGFTHH170 pKa = 6.26PRR172 pKa = 11.84VDD174 pKa = 3.55VTLMMPADD182 pKa = 3.75GTEE185 pKa = 4.26GEE187 pKa = 4.4WAGTIHH193 pKa = 6.81EE194 pKa = 4.63CLEE197 pKa = 4.78GIWGLGQRR205 pKa = 11.84MYY207 pKa = 11.15VYY209 pKa = 10.77AGLEE213 pKa = 4.26GYY215 pKa = 10.21DD216 pKa = 3.69PYY218 pKa = 11.33SAQLYY223 pKa = 10.36ILDD226 pKa = 3.75STQNEE231 pKa = 4.12VRR233 pKa = 11.84GRR235 pKa = 11.84DD236 pKa = 3.79PEE238 pKa = 4.34PPSVDD243 pKa = 4.12RR244 pKa = 11.84ILEE247 pKa = 4.18LMWPDD252 pKa = 3.27VVYY255 pKa = 10.8RR256 pKa = 11.84DD257 pKa = 5.25GSEE260 pKa = 3.95TWDD263 pKa = 3.56GTGDD267 pKa = 3.68AGDD270 pKa = 3.98PGYY273 pKa = 11.11VKK275 pKa = 10.42HH276 pKa = 6.61PQITWYY282 pKa = 10.03DD283 pKa = 3.48GHH285 pKa = 7.71PVMPP289 pKa = 5.45

Molecular weight: 31.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E1QXN3|E1QXN3_OLSUV Putative PTS IIA-like nitrogen-regulatory protein PtsN OS=Olsenella uli (strain ATCC 49627 / DSM 7084 / CIP 109912 / JCM 12494 / NCIMB 702895 / VPI D76D-27C) OX=633147 GN=Olsu_0012 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.62RR10 pKa = 11.84KK11 pKa = 9.56RR12 pKa = 11.84AKK14 pKa = 8.76THH16 pKa = 5.23GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.11GGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.16GRR39 pKa = 11.84KK40 pKa = 8.12QLTVV44 pKa = 2.97

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1739

0

1739

592283

31

2342

340.6

36.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.203 ± 0.078

1.53 ± 0.026

6.552 ± 0.046

6.245 ± 0.052

3.359 ± 0.033

9.006 ± 0.064

1.985 ± 0.022

4.572 ± 0.053

3.047 ± 0.049

9.431 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.632 ± 0.027

2.479 ± 0.031

4.262 ± 0.039

2.755 ± 0.032

6.959 ± 0.063

6.292 ± 0.045

5.327 ± 0.048

8.569 ± 0.051

1.066 ± 0.02

2.73 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski