Dorea longicatena CAG:42

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Dorea; environmental samples

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2196 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7FS58|R7FS58_9FIRM Transcriptional regulator MarR family OS=Dorea longicatena CAG:42 OX=1263074 GN=BN651_01373 PE=4 SV=1
MM1 pKa = 7.35GMYY4 pKa = 9.98DD5 pKa = 3.99EE6 pKa = 5.66KK7 pKa = 11.55VVLCGANSYY16 pKa = 8.09EE17 pKa = 4.0EE18 pKa = 4.81KK19 pKa = 10.95YY20 pKa = 10.77YY21 pKa = 10.91LNPDD25 pKa = 4.09FEE27 pKa = 4.39QLPDD31 pKa = 3.65DD32 pKa = 4.45VKK34 pKa = 11.48NEE36 pKa = 3.94LKK38 pKa = 10.49IMCVLYY44 pKa = 10.47VHH46 pKa = 7.18DD47 pKa = 4.39VGGILTLVFEE57 pKa = 5.17EE58 pKa = 4.08NGEE61 pKa = 4.14LCFEE65 pKa = 4.31VTSDD69 pKa = 4.99DD70 pKa = 4.2FDD72 pKa = 5.49PMFDD76 pKa = 5.62DD77 pKa = 5.13IGSQLKK83 pKa = 10.04IKK85 pKa = 10.16QLQRR89 pKa = 11.84DD90 pKa = 3.96KK91 pKa = 11.42QDD93 pKa = 3.8LLQALQLYY101 pKa = 9.23YY102 pKa = 10.85KK103 pKa = 10.53VFFLGEE109 pKa = 4.23EE110 pKa = 4.26INSEE114 pKa = 4.15EE115 pKa = 4.05

Molecular weight: 13.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7FWD2|R7FWD2_9FIRM Putative iron-sulfur cluster-binding protein OS=Dorea longicatena CAG:42 OX=1263074 GN=BN651_00201 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.79KK9 pKa = 7.6RR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.2VHH16 pKa = 5.65GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.71VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2196

0

2196

679263

31

1513

309.3

34.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.405 ± 0.051

1.459 ± 0.021

5.67 ± 0.05

7.918 ± 0.071

3.825 ± 0.042

7.287 ± 0.046

1.839 ± 0.024

7.634 ± 0.052

7.537 ± 0.049

8.641 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.364 ± 0.026

4.133 ± 0.033

3.207 ± 0.029

3.107 ± 0.03

4.165 ± 0.038

5.304 ± 0.033

5.399 ± 0.031

7.215 ± 0.044

0.813 ± 0.017

4.077 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski