Amino acid dipepetide frequency for Rhodobacteraceae bacterium SB2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.545AlaAla: 13.545 ± 0.156	1.155AlaCys: 1.155 ± 0.038	5.975AlaAsp: 5.975 ± 0.079	6.591AlaGlu: 6.591 ± 0.083	4.47AlaPhe: 4.47 ± 0.081	8.986AlaGly: 8.986 ± 0.101	2.271AlaHis: 2.271 ± 0.047	6.079AlaIle: 6.079 ± 0.083	4.565AlaLys: 4.565 ± 0.071	13.101AlaLeu: 13.101 ± 0.141	3.273AlaMet: 3.273 ± 0.06	3.053AlaAsn: 3.053 ± 0.062	4.618AlaPro: 4.618 ± 0.078	5.352AlaGln: 5.352 ± 0.087	6.138AlaArg: 6.138 ± 0.082	6.103AlaSer: 6.103 ± 0.084	5.005AlaThr: 5.005 ± 0.069	7.092AlaVal: 7.092 ± 0.086	1.301AlaTrp: 1.301 ± 0.037	2.691AlaTyr: 2.691 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	1.207CysAla: 1.207 ± 0.034	0.136CysCys: 0.136 ± 0.013	0.696CysAsp: 0.696 ± 0.022	0.55CysGlu: 0.55 ± 0.028	0.507CysPhe: 0.507 ± 0.022	0.978CysGly: 0.978 ± 0.033	0.278CysHis: 0.278 ± 0.014	0.547CysIle: 0.547 ± 0.025	0.329CysLys: 0.329 ± 0.016	1.068CysLeu: 1.068 ± 0.033	0.222CysMet: 0.222 ± 0.018	0.32CysAsn: 0.32 ± 0.017	0.537CysPro: 0.537 ± 0.025	0.352CysGln: 0.352 ± 0.018	0.503CysArg: 0.503 ± 0.025	0.667CysSer: 0.667 ± 0.027	0.477CysThr: 0.477 ± 0.022	0.725CysVal: 0.725 ± 0.028	0.118CysTrp: 0.118 ± 0.01	0.277CysTyr: 0.277 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	5.899AspAla: 5.899 ± 0.081	0.537AspCys: 0.537 ± 0.023	2.613AspAsp: 2.613 ± 0.093	3.159AspGlu: 3.159 ± 0.063	2.384AspPhe: 2.384 ± 0.051	4.527AspGly: 4.527 ± 0.082	1.351AspHis: 1.351 ± 0.046	3.751AspIle: 3.751 ± 0.058	1.983AspLys: 1.983 ± 0.05	6.506AspLeu: 6.506 ± 0.074	1.616AspMet: 1.616 ± 0.041	1.444AspAsn: 1.444 ± 0.048	3.098AspPro: 3.098 ± 0.054	2.407AspGln: 2.407 ± 0.047	3.269AspArg: 3.269 ± 0.064	2.252AspSer: 2.252 ± 0.05	2.625AspThr: 2.625 ± 0.08	4.002AspVal: 4.002 ± 0.068	1.056AspTrp: 1.056 ± 0.029	1.599AspTyr: 1.599 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	6.963GluAla: 6.963 ± 0.104	0.436GluCys: 0.436 ± 0.019	3.122GluAsp: 3.122 ± 0.057	3.176GluGlu: 3.176 ± 0.057	2.092GluPhe: 2.092 ± 0.04	3.844GluGly: 3.844 ± 0.065	1.236GluHis: 1.236 ± 0.039	4.026GluIle: 4.026 ± 0.062	2.805GluLys: 2.805 ± 0.06	5.16GluLeu: 5.16 ± 0.076	1.748GluMet: 1.748 ± 0.046	2.445GluAsn: 2.445 ± 0.045	2.089GluPro: 2.089 ± 0.049	2.291GluGln: 2.291 ± 0.049	3.432GluArg: 3.432 ± 0.068	2.305GluSer: 2.305 ± 0.044	3.762GluThr: 3.762 ± 0.055	3.67GluVal: 3.67 ± 0.061	0.668GluTrp: 0.668 ± 0.025	1.228GluTyr: 1.228 ± 0.041	0.0GluXaa: 0.0 ± 0.0
Phe	4.243PheAla: 4.243 ± 0.067	0.568PheCys: 0.568 ± 0.025	2.914PheAsp: 2.914 ± 0.055	2.722PheGlu: 2.722 ± 0.061	1.818PhePhe: 1.818 ± 0.05	3.831PheGly: 3.831 ± 0.07	0.81PheHis: 0.81 ± 0.031	2.382PheIle: 2.382 ± 0.054	1.496PheLys: 1.496 ± 0.04	3.975PheLeu: 3.975 ± 0.083	1.052PheMet: 1.052 ± 0.034	1.346PheAsn: 1.346 ± 0.031	1.591PhePro: 1.591 ± 0.037	1.448PheGln: 1.448 ± 0.032	1.83PheArg: 1.83 ± 0.044	2.949PheSer: 2.949 ± 0.057	2.188PheThr: 2.188 ± 0.052	2.725PheVal: 2.725 ± 0.051	0.662PheTrp: 0.662 ± 0.031	1.145PheTyr: 1.145 ± 0.033	0.0PheXaa: 0.0 ± 0.0
Gly	8.644GlyAla: 8.644 ± 0.108	0.963GlyCys: 0.963 ± 0.033	4.054GlyAsp: 4.054 ± 0.101	4.011GlyGlu: 4.011 ± 0.065	3.937GlyPhe: 3.937 ± 0.063	6.375GlyGly: 6.375 ± 0.123	1.86GlyHis: 1.86 ± 0.047	4.564GlyIle: 4.564 ± 0.075	3.385GlyLys: 3.385 ± 0.064	8.82GlyLeu: 8.82 ± 0.106	2.36GlyMet: 2.36 ± 0.046	2.309GlyAsn: 2.309 ± 0.058	3.037GlyPro: 3.037 ± 0.053	3.327GlyGln: 3.327 ± 0.059	4.425GlyArg: 4.425 ± 0.067	4.629GlySer: 4.629 ± 0.081	3.893GlyThr: 3.893 ± 0.086	5.795GlyVal: 5.795 ± 0.079	1.258GlyTrp: 1.258 ± 0.039	2.406GlyTyr: 2.406 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	2.183HisAla: 2.183 ± 0.047	0.281HisCys: 0.281 ± 0.017	1.157HisAsp: 1.157 ± 0.046	1.07HisGlu: 1.07 ± 0.031	0.948HisPhe: 0.948 ± 0.034	1.729HisGly: 1.729 ± 0.047	0.603HisHis: 0.603 ± 0.029	1.327HisIle: 1.327 ± 0.037	0.786HisLys: 0.786 ± 0.029	2.249HisLeu: 2.249 ± 0.049	0.571HisMet: 0.571 ± 0.025	0.62HisAsn: 0.62 ± 0.023	1.371HisPro: 1.371 ± 0.037	0.796HisGln: 0.796 ± 0.025	1.123HisArg: 1.123 ± 0.04	1.252HisSer: 1.252 ± 0.038	0.807HisThr: 0.807 ± 0.031	1.395HisVal: 1.395 ± 0.035	0.334HisTrp: 0.334 ± 0.018	0.594HisTyr: 0.594 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	7.17IleAla: 7.17 ± 0.083	0.882IleCys: 0.882 ± 0.028	3.746IleAsp: 3.746 ± 0.06	4.108IleGlu: 4.108 ± 0.064	2.323IlePhe: 2.323 ± 0.052	5.317IleGly: 5.317 ± 0.071	1.011IleHis: 1.011 ± 0.029	3.312IleIle: 3.312 ± 0.056	2.39IleLys: 2.39 ± 0.047	5.874IleLeu: 5.874 ± 0.076	1.334IleMet: 1.334 ± 0.042	1.984IleAsn: 1.984 ± 0.041	2.498IlePro: 2.498 ± 0.053	1.698IleGln: 1.698 ± 0.043	2.922IleArg: 2.922 ± 0.055	4.294IleSer: 4.294 ± 0.072	3.408IleThr: 3.408 ± 0.062	3.705IleVal: 3.705 ± 0.057	0.877IleTrp: 0.877 ± 0.033	1.463IleTyr: 1.463 ± 0.042	0.0IleXaa: 0.0 ± 0.0
Lys	4.702LysAla: 4.702 ± 0.079	0.286LysCys: 0.286 ± 0.018	2.267LysAsp: 2.267 ± 0.053	2.084LysGlu: 2.084 ± 0.051	1.45LysPhe: 1.45 ± 0.035	2.901LysGly: 2.901 ± 0.056	0.789LysHis: 0.789 ± 0.024	2.822LysIle: 2.822 ± 0.063	1.929LysLys: 1.929 ± 0.048	4.081LysLeu: 4.081 ± 0.073	1.225LysMet: 1.225 ± 0.036	1.578LysAsn: 1.578 ± 0.04	2.144LysPro: 2.144 ± 0.049	1.493LysGln: 1.493 ± 0.037	2.567LysArg: 2.567 ± 0.06	2.775LysSer: 2.775 ± 0.057	2.877LysThr: 2.877 ± 0.054	2.43LysVal: 2.43 ± 0.05	0.495LysTrp: 0.495 ± 0.019	0.874LysTyr: 0.874 ± 0.034	0.0LysXaa: 0.0 ± 0.0
Leu	11.393LeuAla: 11.393 ± 0.113	1.227LeuCys: 1.227 ± 0.035	5.584LeuAsp: 5.584 ± 0.082	5.752LeuGlu: 5.752 ± 0.074	3.944LeuPhe: 3.944 ± 0.081	8.14LeuGly: 8.14 ± 0.099	2.012LeuHis: 2.012 ± 0.051	6.179LeuIle: 6.179 ± 0.08	4.583LeuLys: 4.583 ± 0.074	9.437LeuLeu: 9.437 ± 0.141	2.749LeuMet: 2.749 ± 0.058	3.721LeuAsn: 3.721 ± 0.058	5.141LeuPro: 5.141 ± 0.077	3.818LeuGln: 3.818 ± 0.077	6.137LeuArg: 6.137 ± 0.104	8.037LeuSer: 8.037 ± 0.111	5.489LeuThr: 5.489 ± 0.084	6.282LeuVal: 6.282 ± 0.084	1.287LeuTrp: 1.287 ± 0.046	2.186LeuTyr: 2.186 ± 0.051	0.0LeuXaa: 0.0 ± 0.0
Met	3.236MetAla: 3.236 ± 0.061	0.234MetCys: 0.234 ± 0.014	1.344MetAsp: 1.344 ± 0.032	1.136MetGlu: 1.136 ± 0.033	0.908MetPhe: 0.908 ± 0.031	2.282MetGly: 2.282 ± 0.05	0.554MetHis: 0.554 ± 0.025	1.688MetIle: 1.688 ± 0.044	1.183MetLys: 1.183 ± 0.034	2.627MetLeu: 2.627 ± 0.055	0.833MetMet: 0.833 ± 0.031	0.979MetAsn: 0.979 ± 0.036	1.477MetPro: 1.477 ± 0.037	1.286MetGln: 1.286 ± 0.039	1.878MetArg: 1.878 ± 0.052	1.827MetSer: 1.827 ± 0.047	1.709MetThr: 1.709 ± 0.04	1.855MetVal: 1.855 ± 0.044	0.248MetTrp: 0.248 ± 0.016	0.37MetTyr: 0.37 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	3.721AsnAla: 3.721 ± 0.061	0.405AsnCys: 0.405 ± 0.02	1.714AsnAsp: 1.714 ± 0.066	1.516AsnGlu: 1.516 ± 0.038	1.415AsnPhe: 1.415 ± 0.037	2.759AsnGly: 2.759 ± 0.055	0.663AsnHis: 0.663 ± 0.028	2.235AsnIle: 2.235 ± 0.051	1.186AsnLys: 1.186 ± 0.037	3.479AsnLeu: 3.479 ± 0.059	0.916AsnMet: 0.916 ± 0.031	1.068AsnAsn: 1.068 ± 0.031	2.076AsnPro: 2.076 ± 0.043	1.139AsnGln: 1.139 ± 0.037	1.756AsnArg: 1.756 ± 0.041	1.783AsnSer: 1.783 ± 0.044	1.696AsnThr: 1.696 ± 0.041	2.167AsnVal: 2.167 ± 0.043	0.534AsnTrp: 0.534 ± 0.022	0.906AsnTyr: 0.906 ± 0.029	0.0AsnXaa: 0.0 ± 0.0
Pro	4.503ProAla: 4.503 ± 0.071	0.374ProCys: 0.374 ± 0.019	3.101ProAsp: 3.101 ± 0.057	3.473ProGlu: 3.473 ± 0.057	1.977ProPhe: 1.977 ± 0.043	3.259ProGly: 3.259 ± 0.055	1.008ProHis: 1.008 ± 0.031	2.693ProIle: 2.693 ± 0.055	2.301ProLys: 2.301 ± 0.05	4.539ProLeu: 4.539 ± 0.069	1.216ProMet: 1.216 ± 0.032	1.726ProAsn: 1.726 ± 0.041	1.719ProPro: 1.719 ± 0.048	1.722ProGln: 1.722 ± 0.043	2.055ProArg: 2.055 ± 0.049	2.829ProSer: 2.829 ± 0.052	2.139ProThr: 2.139 ± 0.041	3.354ProVal: 3.354 ± 0.062	0.588ProTrp: 0.588 ± 0.023	1.205ProTyr: 1.205 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	4.658GlnAla: 4.658 ± 0.087	0.26GlnCys: 0.26 ± 0.016	2.156GlnAsp: 2.156 ± 0.052	2.042GlnGlu: 2.042 ± 0.049	1.443GlnPhe: 1.443 ± 0.031	2.815GlnGly: 2.815 ± 0.05	0.815GlnHis: 0.815 ± 0.03	2.663GlnIle: 2.663 ± 0.053	1.912GlnLys: 1.912 ± 0.048	3.418GlnLeu: 3.418 ± 0.066	1.209GlnMet: 1.209 ± 0.035	1.681GlnAsn: 1.681 ± 0.044	1.701GlnPro: 1.701 ± 0.038	1.476GlnGln: 1.476 ± 0.046	2.523GlnArg: 2.523 ± 0.05	2.543GlnSer: 2.543 ± 0.046	2.284GlnThr: 2.284 ± 0.047	2.394GlnVal: 2.394 ± 0.05	0.441GlnTrp: 0.441 ± 0.02	0.78GlnTyr: 0.78 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	6.063ArgAla: 6.063 ± 0.086	0.538ArgCys: 0.538 ± 0.023	3.1ArgAsp: 3.1 ± 0.059	2.824ArgGlu: 2.824 ± 0.056	2.495ArgPhe: 2.495 ± 0.05	3.659ArgGly: 3.659 ± 0.064	1.304ArgHis: 1.304 ± 0.036	3.241ArgIle: 3.241 ± 0.054	2.455ArgLys: 2.455 ± 0.054	6.195ArgLeu: 6.195 ± 0.102	1.622ArgMet: 1.622 ± 0.041	1.794ArgAsn: 1.794 ± 0.042	2.548ArgPro: 2.548 ± 0.047	2.291ArgGln: 2.291 ± 0.047	3.517ArgArg: 3.517 ± 0.07	3.335ArgSer: 3.335 ± 0.064	1.991ArgThr: 1.991 ± 0.046	3.793ArgVal: 3.793 ± 0.065	0.734ArgTrp: 0.734 ± 0.027	1.444ArgTyr: 1.444 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	6.767SerAla: 6.767 ± 0.078	0.643SerCys: 0.643 ± 0.027	3.85SerAsp: 3.85 ± 0.067	3.608SerGlu: 3.608 ± 0.068	2.85SerPhe: 2.85 ± 0.054	5.903SerGly: 5.903 ± 0.107	1.256SerHis: 1.256 ± 0.037	3.303SerIle: 3.303 ± 0.053	2.51SerLys: 2.51 ± 0.054	6.155SerLeu: 6.155 ± 0.086	1.5SerMet: 1.5 ± 0.038	1.963SerAsn: 1.963 ± 0.047	2.481SerPro: 2.481 ± 0.053	2.328SerGln: 2.328 ± 0.048	2.832SerArg: 2.832 ± 0.054	3.615SerSer: 3.615 ± 0.088	2.915SerThr: 2.915 ± 0.059	4.187SerVal: 4.187 ± 0.063	0.727SerTrp: 0.727 ± 0.026	1.643SerTyr: 1.643 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	5.561ThrAla: 5.561 ± 0.083	0.483ThrCys: 0.483 ± 0.021	2.717ThrAsp: 2.717 ± 0.059	2.573ThrGlu: 2.573 ± 0.058	1.967ThrPhe: 1.967 ± 0.052	4.899ThrGly: 4.899 ± 0.073	1.163ThrHis: 1.163 ± 0.03	2.981ThrIle: 2.981 ± 0.071	1.918ThrLys: 1.918 ± 0.043	5.607ThrLeu: 5.607 ± 0.084	1.205ThrMet: 1.205 ± 0.033	1.588ThrAsn: 1.588 ± 0.048	3.054ThrPro: 3.054 ± 0.054	2.014ThrGln: 2.014 ± 0.042	2.743ThrArg: 2.743 ± 0.054	3.133ThrSer: 3.133 ± 0.066	2.627ThrThr: 2.627 ± 0.058	3.466ThrVal: 3.466 ± 0.068	0.603ThrTrp: 0.603 ± 0.026	1.288ThrTyr: 1.288 ± 0.041	0.0ThrXaa: 0.0 ± 0.0
Val	7.019ValAla: 7.019 ± 0.081	0.631ValCys: 0.631 ± 0.026	3.738ValAsp: 3.738 ± 0.061	4.071ValGlu: 4.071 ± 0.064	3.078ValPhe: 3.078 ± 0.063	4.634ValGly: 4.634 ± 0.081	1.255ValHis: 1.255 ± 0.034	4.542ValIle: 4.542 ± 0.067	2.664ValLys: 2.664 ± 0.055	6.929ValLeu: 6.929 ± 0.098	2.022ValMet: 2.022 ± 0.047	2.207ValAsn: 2.207 ± 0.052	2.85ValPro: 2.85 ± 0.056	2.234ValGln: 2.234 ± 0.046	2.993ValArg: 2.993 ± 0.057	4.365ValSer: 4.365 ± 0.076	3.888ValThr: 3.888 ± 0.069	4.684ValVal: 4.684 ± 0.075	0.795ValTrp: 0.795 ± 0.032	1.496ValTyr: 1.496 ± 0.04	0.0ValXaa: 0.0 ± 0.0
Trp	1.328TrpAla: 1.328 ± 0.039	0.139TrpCys: 0.139 ± 0.011	0.658TrpAsp: 0.658 ± 0.028	0.6TrpGlu: 0.6 ± 0.025	0.583TrpPhe: 0.583 ± 0.023	1.009TrpGly: 1.009 ± 0.03	0.347TrpHis: 0.347 ± 0.017	0.747TrpIle: 0.747 ± 0.026	0.517TrpLys: 0.517 ± 0.02	1.421TrpLeu: 1.421 ± 0.043	0.41TrpMet: 0.41 ± 0.021	0.503TrpAsn: 0.503 ± 0.024	0.62TrpPro: 0.62 ± 0.025	0.741TrpGln: 0.741 ± 0.027	0.912TrpArg: 0.912 ± 0.032	0.841TrpSer: 0.841 ± 0.031	0.603TrpThr: 0.603 ± 0.026	0.858TrpVal: 0.858 ± 0.03	0.23TrpTrp: 0.23 ± 0.016	0.312TrpTyr: 0.312 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.558TyrAla: 2.558 ± 0.05	0.316TyrCys: 0.316 ± 0.018	1.574TyrAsp: 1.574 ± 0.046	1.43TyrGlu: 1.43 ± 0.038	1.109TyrPhe: 1.109 ± 0.036	2.206TyrGly: 2.206 ± 0.049	0.609TyrHis: 0.609 ± 0.025	1.234TyrIle: 1.234 ± 0.032	0.872TyrLys: 0.872 ± 0.028	2.458TyrLeu: 2.458 ± 0.043	0.57TyrMet: 0.57 ± 0.023	0.803TyrAsn: 0.803 ± 0.03	1.13TyrPro: 1.13 ± 0.032	1.0TyrGln: 1.0 ± 0.033	1.388TyrArg: 1.388 ± 0.038	1.502TyrSer: 1.502 ± 0.034	1.208TyrThr: 1.208 ± 0.039	1.518TyrVal: 1.518 ± 0.04	0.423TyrTrp: 0.423 ± 0.02	0.691TyrTyr: 0.691 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3489 proteins (1066040 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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