Aquiflexum balticum DSM 16537

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cyclobacteriaceae; Aquiflexum; Aquiflexum balticum

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4889 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W2H4H1|A0A1W2H4H1_9BACT Isocitrate dehydrogenase (NADP(+)) OS=Aquiflexum balticum DSM 16537 OX=758820 GN=SAMN00777080_2398 PE=3 SV=1
MM1 pKa = 7.28LALVAFMVACSQFEE15 pKa = 4.14AQGPDD20 pKa = 3.58DD21 pKa = 4.42VNLEE25 pKa = 4.09NAEE28 pKa = 4.11EE29 pKa = 3.93LASAFSMDD37 pKa = 3.78PYY39 pKa = 11.62GLGLEE44 pKa = 4.35NARR47 pKa = 11.84VPLEE51 pKa = 4.29GKK53 pKa = 8.56GTASEE58 pKa = 4.16PVSDD62 pKa = 5.0GGITPYY68 pKa = 10.38IITGTSGGGNRR79 pKa = 11.84TCDD82 pKa = 3.59EE83 pKa = 3.96VATAYY88 pKa = 10.61GIGSFEE94 pKa = 4.63NSFEE98 pKa = 4.32QINTPFTNNTATFGLLTATTDD119 pKa = 3.39GTFVSWSIEE128 pKa = 4.01VPEE131 pKa = 5.35GYY133 pKa = 9.51CVKK136 pKa = 10.64YY137 pKa = 10.18VAAVVKK143 pKa = 10.42GGNDD147 pKa = 3.22ANVYY151 pKa = 9.94FYY153 pKa = 10.86EE154 pKa = 5.91GDD156 pKa = 3.39VSGDD160 pKa = 3.29SGLASPGAGGSGGAASLSNLRR181 pKa = 11.84FCYY184 pKa = 9.85TLEE187 pKa = 4.36EE188 pKa = 4.73KK189 pKa = 10.43PDD191 pKa = 3.66APIVEE196 pKa = 4.37GDD198 pKa = 3.71EE199 pKa = 4.23EE200 pKa = 5.5CFVEE204 pKa = 5.25DD205 pKa = 3.6LVLRR209 pKa = 11.84ATLADD214 pKa = 3.37GVTIPEE220 pKa = 5.14GYY222 pKa = 8.07TLQWYY227 pKa = 9.83KK228 pKa = 10.77KK229 pKa = 9.55VNGEE233 pKa = 4.02YY234 pKa = 9.96VALGEE239 pKa = 4.3GEE241 pKa = 4.52EE242 pKa = 4.72PILDD246 pKa = 3.71EE247 pKa = 4.75VGSIDD252 pKa = 3.88YY253 pKa = 10.5YY254 pKa = 10.72AAFVNGCSSEE264 pKa = 4.01MSNAATLTINPLPGPPTGGDD284 pKa = 3.15QDD286 pKa = 5.09EE287 pKa = 4.59EE288 pKa = 4.57VCDD291 pKa = 4.9FSKK294 pKa = 10.05LTATVKK300 pKa = 10.46DD301 pKa = 3.89VPDD304 pKa = 3.94GVSIVWYY311 pKa = 9.27DD312 pKa = 3.27APTGGNVVTDD322 pKa = 4.15PSLVYY327 pKa = 10.42DD328 pKa = 4.0PSGDD332 pKa = 3.63EE333 pKa = 4.05VQTATFYY340 pKa = 11.62AEE342 pKa = 4.06AVNDD346 pKa = 3.51EE347 pKa = 4.65TEE349 pKa = 4.47CVSATRR355 pKa = 11.84TMVTLTLRR363 pKa = 11.84PCTRR367 pKa = 11.84EE368 pKa = 4.03CYY370 pKa = 10.15DD371 pKa = 4.37DD372 pKa = 4.48EE373 pKa = 4.78SATGRR378 pKa = 11.84GNAFTNRR385 pKa = 11.84RR386 pKa = 11.84PATWFQWNTRR396 pKa = 11.84GEE398 pKa = 4.8LITGVDD404 pKa = 3.68LVYY407 pKa = 10.88GRR409 pKa = 11.84QLEE412 pKa = 4.44KK413 pKa = 10.8AGSVTISGPDD423 pKa = 3.04GGFRR427 pKa = 11.84TITVTLDD434 pKa = 2.98PGFRR438 pKa = 11.84FAQDD442 pKa = 2.82ADD444 pKa = 3.84GNIVSGALKK453 pKa = 10.55VFASNTQFTQFSGFSGALQSNTATIRR479 pKa = 11.84VPDD482 pKa = 3.3TGFYY486 pKa = 10.05FVHH489 pKa = 7.02GDD491 pKa = 3.25IEE493 pKa = 4.54RR494 pKa = 11.84KK495 pKa = 8.89IDD497 pKa = 4.38CPIADD502 pKa = 3.47

Molecular weight: 53.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W2H4E2|A0A1W2H4E2_9BACT Uncharacterized protein OS=Aquiflexum balticum DSM 16537 OX=758820 GN=SAMN00777080_2427 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.78HH16 pKa = 3.85GFRR19 pKa = 11.84LRR21 pKa = 11.84MSTANGRR28 pKa = 11.84RR29 pKa = 11.84VLKK32 pKa = 10.41SRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.19GRR39 pKa = 11.84HH40 pKa = 5.41KK41 pKa = 10.43LTVSSEE47 pKa = 4.01KK48 pKa = 9.88TLKK51 pKa = 10.51KK52 pKa = 10.72

Molecular weight: 6.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4889

0

4889

1724006

29

6369

352.6

39.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.171 ± 0.038

0.686 ± 0.011

5.405 ± 0.029

6.907 ± 0.035

5.578 ± 0.028

7.045 ± 0.04

1.824 ± 0.016

7.69 ± 0.031

7.046 ± 0.044

9.67 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.436 ± 0.019

5.504 ± 0.036

3.938 ± 0.019

3.513 ± 0.019

3.913 ± 0.024

6.583 ± 0.026

5.024 ± 0.037

6.053 ± 0.028

1.288 ± 0.013

3.726 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski